LOCUS       NP_032885                707 aa            linear   ROD 27-JUL-2024
DEFINITION  protein kinase C theta type isoform 1 [Mus musculus].
ACCESSION   NP_032885
VERSION     NP_032885.1
DBSOURCE    REFSEQ: accession NM_008859.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 707)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 707)
  AUTHORS   Gaspar,R.C., Lyu,K., Hubbard,B.T., Leitner,B.P., Luukkonen,P.K.,
            Hirabara,S.M., Sakuma,I., Nasiri,A., Zhang,D., Kahn,M., Cline,G.W.,
            Pauli,J.R., Perry,R.J., Petersen,K.F. and Shulman,G.I.
  TITLE     Distinct subcellular localisation of intramyocellular lipids and
            reduced PKCepsilon/PKCtheta; activity preserve muscle insulin
            sensitivity in exercise-trained mice
  JOURNAL   Diabetologia 66 (3), 567-578 (2023)
   PUBMED   36456864
  REMARK    GeneRIF: Distinct subcellular localisation of intramyocellular
            lipids and reduced PKCepsilon/PKCtheta; activity preserve muscle
            insulin sensitivity in exercise-trained mice.
REFERENCE   3  (residues 1 to 707)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   4  (residues 1 to 707)
  AUTHORS   Park,J., Lee,S.Y., Jeon,Y., Kim,K.M., Lee,J.K., Ko,J., Park,E.J.,
            Yoon,J.S., Kang,B.E., Ryu,D., Lee,H., Shin,S.J., Go,H. and Lee,C.W.
  TITLE     The Pellino1-PKCtheta; Signaling Axis Is an Essential Target for
            Improving Antitumor CD8+ T-lymphocyte Function
  JOURNAL   Cancer Immunol Res 10 (3), 327-342 (2022)
   PUBMED   35058288
REFERENCE   5  (residues 1 to 707)
  AUTHORS   He,Y., Yang,Z., Zhao,C.S., Xiao,Z., Gong,Y., Li,Y.Y., Chen,Y.,
            Du,Y., Feng,D., Altman,A. and Li,Y.
  TITLE     T-cell receptor (TCR) signaling promotes the assembly of
            RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via
            PKC-theta;-mediated phosphorylation of RanGAP1
  JOURNAL   Elife 10, e67123 (2021)
   PUBMED   34110283
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 707)
  AUTHORS   Zappelli,F., Willems,D., Osada,S., Ohno,S., Wetsel,W.C.,
            Molinaro,M., Cossu,G. and Bouche,M.
  TITLE     The inhibition of differentiation caused by TGFbeta in fetal
            myoblasts is dependent upon selective expression of PKCtheta: a
            possible molecular basis for myoblast diversification during limb
            histogenesis
  JOURNAL   Dev Biol 180 (1), 156-164 (1996)
   PUBMED   8948582
REFERENCE   7  (residues 1 to 707)
  AUTHORS   Bareggi,R., Grill,V., Zweyer,M., Narducci,P. and Martelli,A.M.
  TITLE     Distribution of the extended family of protein kinase C isoenzymes
            in fetal organs of mice: an immunohistochemical study
  JOURNAL   Cell Tissue Res 280 (3), 617-625 (1995)
   PUBMED   7606770
REFERENCE   8  (residues 1 to 707)
  AUTHORS   Hilgenberg,L. and Miles,K.
  TITLE     Developmental regulation of a protein kinase C isoform localized in
            the neuromuscular junction
  JOURNAL   J Cell Sci 108 (Pt 1), 51-61 (1995)
   PUBMED   7738116
REFERENCE   9  (residues 1 to 707)
  AUTHORS   Mischak,H., Goodnight,J., Henderson,D.W., Osada,S., Ohno,S. and
            Mushinski,J.F.
  TITLE     Unique expression pattern of protein kinase C-theta: high mRNA
            levels in normal mouse testes and in T-lymphocytic cells and
            neoplasms
  JOURNAL   FEBS Lett 326 (1-3), 51-55 (1993)
   PUBMED   8325388
REFERENCE   10 (residues 1 to 707)
  AUTHORS   Osada,S., Mizuno,K., Saido,T.C., Suzuki,K., Kuroki,T. and Ohno,S.
  TITLE     A new member of the protein kinase C family, nPKC theta,
            predominantly expressed in skeletal muscle
  JOURNAL   Mol Cell Biol 12 (9), 3930-3938 (1992)
   PUBMED   1508194
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928715.11 and AL929020.10.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D11091.1, SRR13861890.193763.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 8.42 cM"
     Protein         1..707
                     /product="protein kinase C theta type isoform 1"
                     /EC_number="2.7.11.13"
                     /note="protein kinase C theta type; nPKC-theta; PKC theta"
                     /calculated_mol_wt=81442
     Site            90
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by LCK.
                     /evidence=ECO:0000250|UniProtKB:Q04759; propagated from
                     UniProtKB/Swiss-Prot (Q02111.1)"
     Region          153..213
                     /region_name="C1_nPKC_theta-like_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in novel protein kinase C (nPKC) theta,
                     delta, and similar proteins; cd20834"
                     /db_xref="CDD:410384"
     Site            order(165..171,179..183,186)
                     /site_type="other"
                     /note="putative DAG/PE binding site [chemical binding]"
                     /db_xref="CDD:410384"
     Site            219
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:Q04759; propagated from
                     UniProtKB/Swiss-Prot (Q02111.1)"
     Region          232..281
                     /region_name="C1_nPKC_theta-like_rpt2"
                     /note="second protein kinase C conserved region 1 (C1
                     domain) found in novel protein kinase C (nPKC) theta,
                     delta, and similar proteins; cd20837"
                     /db_xref="CDD:410387"
     Site            order(237..243,251..255,258)
                     /site_type="other"
                     /note="DAG/PE binding site [chemical binding]"
                     /db_xref="CDD:410387"
     Region          327..365
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q02111.1)"
     Site            348
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q04759; propagated from
                     UniProtKB/Swiss-Prot (Q02111.1)"
     Region          374..704
                     /region_name="STKc_nPKC_theta"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Novel Protein Kinase C theta; cd05619"
                     /db_xref="CDD:270770"
     Site            order(386..390,394,407,409,442,458..459,461,465,467,504,
                     506,508..509,511,521..522,525,539..544,571,577,580)
                     /site_type="active"
                     /db_xref="CDD:270770"
     Site            order(386..390,394,407,409,442,459..461,465,504,506,
                     508..509,511,521..522)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270770"
     Site            order(390,465,467,504,506,508,525,539..544,571,577,580)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270770"
     Site            521..544
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270770"
     Site            538
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by PDPK1.
                     /evidence=ECO:0000250|UniProtKB:Q04759; propagated from
                     UniProtKB/Swiss-Prot (Q02111.1)"
     Site            676
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:Q04759, ECO:0000255;
                     propagated from UniProtKB/Swiss-Prot (Q02111.1)"
     Site            676
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270770"
     Site            685
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q04759; propagated from
                     UniProtKB/Swiss-Prot (Q02111.1)"
     Site            691..696
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270770"
     Site            695
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:Q04759, ECO:0000255;
                     propagated from UniProtKB/Swiss-Prot (Q02111.1)"
     CDS             1..707
                     /gene="Prkcq"
                     /gene_synonym="A130035A12Rik; PKC-0; PKC-theta; Pkcq;
                     PKCtheta"
                     /coded_by="NM_008859.3:360..2483"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS15682.1"
                     /db_xref="GeneID:18761"
                     /db_xref="MGI:MGI:97601"
ORIGIN      
        1 mspflrigls nfdcgtcqac qgeavnpyca vlvkeyvese ngqmyiqkkp tmyppwdstf
       61 dahinkgrvm qiivkgknvd lisettvely slaercrknn grteiwlelk pqgrmlmnar
      121 yflemsdtkd msefenegff alhqrrgaik qakvhhvkch eftatffpqp tfcsvchefv
      181 wglnkqgyqc rqcnaaihkk cidkviakct gsainsretm fhkerfkidm phrfkvynyk
      241 sptfcehcgt llwglarqgl kcdacgmnvh hrcqtkvanl cginqklmae alamiestqq
      301 arslrdsehi fregpveigl pcstknetrp pcvptpgkre pqgiswdspl dgsnksagpp
      361 epevsmrrts lqlklkiddf ilhkmlgkgs fgkvflaefk rtnqffaika lkkdvvlmdd
      421 dvectmvekr vlslawehpf lthmfctfqt kenlffvmey lnggdlmyhi qschkfdlsr
      481 atfyaaevil glqflhskgi vyrdlkldni lldrdghiki adfgmckenm lgdaktntfc
      541 gtpdyiapei llgqkynhsv dwwsfgvlvy emligqspfh gqdeeelfhs irmdnpfypr
      601 wlereakdll vklfvrepek rlgvrgdirq hplfreinwe elerkeidpp frpkvkspyd
      661 csnfdkefls ekprlsfadr alinsmdqnm fsnfsfinpg metlics
//
