LOCUS       NM_008856               3546 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus protein kinase C, eta (Prkch), transcript variant 1,
            mRNA.
ACCESSION   NM_008856
VERSION     NM_008856.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Colgan,L.A., Parra-Bueno,P., Holman,H.L., Tu,X., Jain,A.,
            Calubag,M.F., Misler,J.A., Gary,C., Oz,G., Suponitsky-Kroyter,I.,
            Okaz,E. and Yasuda,R.
  TITLE     Dual Regulation of Spine-Specific and Synapse-to-Nucleus Signaling
            by PKCdelta during Plasticity
  JOURNAL   J Neurosci 43 (30), 5432-5447 (2023)
   PUBMED   37277178
REFERENCE   3  (bases 1 to 3546)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   4  (bases 1 to 3546)
  AUTHORS   Shemirani,R., Lin,G., Abduweli Uyghurturk,D., Le,M. and Nakano,Y.
  TITLE     An miRNA derived from amelogenin exon4 regulates expression of
            transcription factor Runx2 by directly targeting upstream
            activators Nfia and Prkch
  JOURNAL   J Biol Chem 298 (5), 101807 (2022)
   PUBMED   35271849
  REMARK    GeneRIF: An miRNA derived from amelogenin exon4 regulates
            expression of transcription factor Runx2 by directly targeting
            upstream activators Nfia and Prkch.
REFERENCE   5  (bases 1 to 3546)
  AUTHORS   Liu,H.Y., Pedros,C., Kong,K.F., Canonigo-Balancio,A.J., Xue,W. and
            Altman,A.
  TITLE     Leveraging the Treg-intrinsic CTLA4-PKCeta signaling pathway for
            cancer immunotherapy
  JOURNAL   J Immunother Cancer 9 (9) (2021)
   PUBMED   34588224
REFERENCE   6  (bases 1 to 3546)
  AUTHORS   Zappelli,F., Willems,D., Osada,S., Ohno,S., Wetsel,W.C.,
            Molinaro,M., Cossu,G. and Bouche,M.
  TITLE     The inhibition of differentiation caused by TGFbeta in fetal
            myoblasts is dependent upon selective expression of PKCtheta: a
            possible molecular basis for myoblast diversification during limb
            histogenesis
  JOURNAL   Dev Biol 180 (1), 156-164 (1996)
   PUBMED   8948582
REFERENCE   7  (bases 1 to 3546)
  AUTHORS   Frevert,E.U. and Kahn,B.B.
  TITLE     Protein kinase C isoforms epsilon, eta, delta and zeta in murine
            adipocytes: expression, subcellular localization and
            tissue-specific regulation in insulin-resistant states
  JOURNAL   Biochem J 316 (Pt 3) (Pt 3), 865-871 (1996)
   PUBMED   8670164
REFERENCE   8  (bases 1 to 3546)
  AUTHORS   Sorensen,A.B., Duch,M., Amtoft,H.W., Jorgensen,P. and Pedersen,F.S.
  TITLE     Sequence tags of provirus integration sites in DNAs of tumors
            induced by the murine retrovirus SL3-3
  JOURNAL   J Virol 70 (6), 4063-4070 (1996)
   PUBMED   8648744
REFERENCE   9  (bases 1 to 3546)
  AUTHORS   Bareggi,R., Grill,V., Zweyer,M., Narducci,P. and Martelli,A.M.
  TITLE     Distribution of the extended family of protein kinase C isoenzymes
            in fetal organs of mice: an immunohistochemical study
  JOURNAL   Cell Tissue Res 280 (3), 617-625 (1995)
   PUBMED   7606770
REFERENCE   10 (bases 1 to 3546)
  AUTHORS   Osada,S., Mizuno,K., Saido,T.C., Akita,Y., Suzuki,K., Kuroki,T. and
            Ohno,S.
  TITLE     A phorbol ester receptor/protein kinase, nPKC eta, a new member of
            the protein kinase C family predominantly expressed in lung and
            skin
  JOURNAL   J Biol Chem 265 (36), 22434-22440 (1990)
   PUBMED   2266135
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124179.4, AK145817.1,
            AK164044.1 and BF462787.1.
            
            On Sep 18, 2015 this sequence version replaced NM_008856.3.
            
            Summary: Protein kinase C (PKC) is a family of serine- and
            threonine-specific protein kinases that can be activated by calcium
            and the second messenger diacylglycerol. PKC family members
            phosphorylate a wide variety of protein targets and are known to be
            involved in diverse cellular signaling pathways. PKC family members
            also serve as major receptors for phorbol esters, a class of tumor
            promoters. Each member of the PKC family has a specific expression
            profile and is believed to play a distinct role in cells. The
            protein encoded by this gene is one of the PKC family members. It
            is a calcium-independent and phospholipids-dependent protein
            kinase. It is predominantly expressed in epithelial tissues and has
            been shown to reside specifically in the cell nucleus. This protein
            kinase can regulate keratinocyte differentiation by activating the
            MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that
            targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also
            found to mediate the transcription activation of the
            transglutaminase 1 (TGM1) gene. Mutations in the human gene are
            associated with susceptibility to cerebral infarction. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031121.1, AK164044.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC124179.4         135359-135359
            2-365               AK145817.1         3-366
            366-3537            AK164044.1         320-3491
            3538-3546           BF462787.1         16-24               c
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 30.81 cM"
     gene            1..3546
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /note="protein kinase C, eta"
                     /db_xref="GeneID:18755"
                     /db_xref="MGI:MGI:97600"
     exon            1..730
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     CDS             368..2419
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /EC_number="2.7.11.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase C eta type; PKC-L; nPKC-eta"
                     /codon_start=1
                     /product="protein kinase C eta type isoform 1"
                     /protein_id="NP_032882.2"
                     /db_xref="CCDS:CCDS25976.1"
                     /db_xref="GeneID:18755"
                     /db_xref="MGI:MGI:97600"
                     /translation="MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPY
                     LTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCT
                     LQFQELLRTAGTSDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQR
                     AMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLI
                     VTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQC
                     KICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKE
                     GSKEGNGIGVNSSSRFGIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVIL
                     QDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRR
                     FDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNG
                     VTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAI
                     LNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWAQLNH
                     RQLEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQ
                     L"
     exon            731..794
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            795..945
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            946..980
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1069
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1199
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1327
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1471
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1645
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1800
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1939
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2128
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2272
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..3546
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3518..3523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /note="hexamer: AATAAA"
     polyA_site      3546
                     /gene="Prkch"
                     /gene_synonym="Pkch"
                     /note="major polyA site"
ORIGIN      
        1 agagacgcca aagctggagc tagccggacg ggtcgctcct gctctccggg gagactatct
       61 ggccggcccc ggagaagggg cgagccccgc gcgctccaga aggacgatcg ggagcccagc
      121 agcccgccga gggctcggag cccggctccc ctcctttcca cctcaggaga aagggcggga
      181 gggaaggagg ggaggggaag atccctccga ggaggcggaa tggccggtcg cagggggctc
      241 gggcgctgct tcttcccaga gctgcctcca cgcccgtggc agcggagagg actgccctga
      301 gcccgggctc cgggttcccc agtgccagcc agcgcggccc cctcggggct ccggcagcag
      361 cgccggcatg tcgtccggca cgatgaagtt caatggctat ctgagggtcc gcatcggaga
      421 ggctgtaggg ctgcagccca cccgctggtc cctgcggcac tcgctcttca aaaagggcca
      481 ccagctgctg gacccctacc tgacggtgag cgtagaccag gtacgcgtgg gccagaccag
      541 cacaaagcag aagaccaaca aacccaccta caacgaggag ttctgcgcca atgtcaccga
      601 cggcggccac ctggagctag ccgtcttcca cgagacgccc ctgggttatg accactttgt
      661 ggccaactgc acgctgcagt tccaggagct gttgcgcacg gctggtacct cggacacctt
      721 cgagggctgg gtggatctgg agcctgaggg gaaagtgttt gtggtaataa ccctaacagg
      781 gagtttcact gaagccactc tccagagaga ccgcatcttc aagcatttta ccaggaagcg
      841 ccaaagggct atgcgaagac gagtccatca agtgaacgga cataagttca tggccacgta
      901 cctgaggcag cccacctact gctctcattg ccgagagttc atctggggag tatttgggaa
      961 acagggttat caatgccaag tgtgcacctg cgtcgtccat aaacgctgcc atcatctaat
     1021 tgttacagcc tgcacttgcc aaaacaatat taacaaagtg gatgccaaga ttgcagaaca
     1081 gcggtttggc atcaacatcc cacacaagtt caacgttcac aattacaagg tgcccacgtt
     1141 ctgtgaccac tgtggctccc tgctctgggg gataatgcga caaggacttc agtgtaaaat
     1201 atgtaagatg aatgtacata ttcggtgtca ggcgaacgtg gccccaaact gcggggtgaa
     1261 tgccgtggag cttgccaaga ccctggcagg gatgggtctc caacccggaa atatttctcc
     1321 aacctcgaaa ctcatttcca ggtcgacact aagacggcag ggaaaggagg gctccaaaga
     1381 aggaaatggg atcggtgtta actcttccag cagattcggc atcgacaact ttgagttcat
     1441 ccgggtgttg gggaagggga gcttcgggaa ggtgatgctt gccaggataa aggagacagg
     1501 agaactgtac gccgtgaagg tgctgaagaa ggacgtgatt ctgcaggatg atgatgtaga
     1561 gtgcaccatg actgagaaga ggatcctgtc cttggcccgc aaccacccct tcctcaccca
     1621 gctcttctgc tgctttcaga ctccagaccg tctgttcttt gtcatggagt ttgtgaacgg
     1681 aggcgacctg atgttccaca tccaaaagtc ccgtcgtttc gatgaagccc gtgctcgttt
     1741 ctacgccgcg gagatcattt ctgcactcat gttcctacat gagaaaggta tcatctatag
     1801 agacttgaaa ctggacaatg tgctattgga ccacgaaggt cactgtaaac tggccgactt
     1861 tggaatgtgc aaggagggga tttgtaatgg ggtcaccaca gccaccttct gcggtacacc
     1921 cgactacatt gccccggaga tccttcagga gatgctgtat ggacctgcag tagactggtg
     1981 ggccatgggt gtgttgcttt atgagatgct gtgcggacat gcgccctttg aggctgaaaa
     2041 tgaagatgac ctttttgagg ccatactgaa tgatgaagtc gtctacccca cctggctcca
     2101 cgaagatgcc acagggatcc tcaagtcttt catgaccaag aaccccacca tgcgcttggg
     2161 cagcctgact cagggaggag agcatgagat cctgagacac cctttcttta aggaaatcga
     2221 ctgggcccag ttgaaccatc gccagttaga gccgcctttc cgacctagaa tcaaatcccg
     2281 agaagatgtc agcaattttg acccagactt tataaaagaa gagcccgtct taactccgat
     2341 tgatgaggga catcttccta tgattaacca ggatgagttt agaaactttt cctatgtgtc
     2401 accggaattg caactgtagc cttatgggga gtcagaacca aaggggaagg tggatttctc
     2461 caggaatttc ttctgtggga attccctagc atccgcctta gaacaccagc ttcaccttca
     2521 tggagcaaaa tgttcacaac tctgcaaagg gcggagcctc aagagagctg ctctctcaag
     2581 tccccaggag ccatggtaca tttcagtaat cgatactgag ataagatgtt acaaagatgt
     2641 gcactgctca acccatgagt ctccagggct ctttcaggct tggaggaaag gacagaagcc
     2701 aaaggaagag atgctgggaa ggatacagga gatcctaggg attataagcc aaacaggctt
     2761 cctttttttt tttaacttca agagacaaat ctactttctg tggagcactc tacactcttc
     2821 tttaagccaa tatttcagtc tacaaaagat taaatgttga tgaagaagtc tctctaggga
     2881 aaagaacggc attctaagta gaaggatgcg ctttgaagaa actgcagatg ggcgtccttc
     2941 aagctcccaa cctaaaccca gtgttgagag cggaggttgg acaccacgac aaccgtcagt
     3001 gtcttgagtc tcttcctctt cctttcagta actagatatt tattgagtgt caaataaaaa
     3061 ggtgccataa tcttcagtag cgtacacagt agagtcatga cgcttttaca catcctgggg
     3121 tccacccaat ggtgttcacg ttttgggaaa gtcctctcca cattgcatag gtctgtcttc
     3181 tttgtctcct gttcatagaa gggccaccac cacagttgtg cggtcagcag gcaggcttct
     3241 gttgcatgcc acccgaggac tggtggaagc gaatcacccg cacatacttc atctcgggct
     3301 gactcgttgg tgaagaactc tgcactgttc ccatccatga acacgtgtgt ggcggatcct
     3361 ccagtcagtc tatttaagtt atgttttcag agacaaagaa ggttgtttga tatacacatt
     3421 aataattaac aatgggcctt gcaaacaagg ctgacgacaa aggtgtacct tttcgtgtgt
     3481 aaccatatat acagttgaaa agttttatct ggctgaaaat aaaggcattt ttgtagcaaa
     3541 aggaaa
//
