LOCUS       NP_032950                402 aa            linear   ROD 08-JUN-2024
DEFINITION  cAMP-dependent protein kinase type II-alpha regulatory subunit [Mus
            musculus].
ACCESSION   NP_032950
VERSION     NP_032950.1
DBSOURCE    REFSEQ: accession NM_008924.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 402)
  AUTHORS   Wei,L., Zhang,R., Zhang,J., Li,J., Kong,D., Wang,Q., Fang,J. and
            Wang,L.
  TITLE     PRKAR2A deficiency protects mice from experimental colitis by
            increasing IFN-stimulated gene expression and modulating the
            intestinal microbiota
  JOURNAL   Mucosal Immunol 14 (6), 1282-1294 (2021)
   PUBMED   34349238
  REMARK    GeneRIF: PRKAR2A deficiency protects mice from experimental colitis
            by increasing IFN-stimulated gene expression and modulating the
            intestinal microbiota.
REFERENCE   2  (residues 1 to 402)
  AUTHORS   London,E., Wester,J.C., Bloyd,M., Bettencourt,S., McBain,C.J. and
            Stratakis,C.A.
  TITLE     Loss of habenular Prkar2a reduces hedonic eating and increases
            exercise motivation
  JOURNAL   JCI Insight 5 (23), 141670 (2020)
   PUBMED   33141766
  REMARK    GeneRIF: Loss of habenular Prkar2a reduces hedonic eating and
            increases exercise motivation.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 402)
  AUTHORS   Isensee,J., Kaufholz,M., Knape,M.J., Hasenauer,J., Hammerich,H.,
            Gonczarowska-Jorge,H., Zahedi,R.P., Schwede,F., Herberg,F.W. and
            Hucho,T.
  TITLE     PKA-RII subunit phosphorylation precedes activation by cAMP and
            regulates activity termination
  JOURNAL   J Cell Biol 217 (6), 2167-2184 (2018)
   PUBMED   29615473
  REMARK    GeneRIF: RII phosphorylation precedes cAMP binding and controls the
            inactivation by modulating the reassociation involving the
            coordinated action of phosphodiesterases and phosphatases.
REFERENCE   4  (residues 1 to 402)
  AUTHORS   Kong,D., Shen,Y., Liu,G., Zuo,S., Ji,Y., Lu,A., Nakamura,M.,
            Lazarus,M., Stratakis,C.A., Breyer,R.M. and Yu,Y.
  TITLE     PKA regulatory IIalpha subunit is essential for PGD2-mediated
            resolution of inflammation
  JOURNAL   J Exp Med 213 (10), 2209-2226 (2016)
   PUBMED   27621415
  REMARK    GeneRIF: PGD2-DP1 axis-induced M2 polarization facilitates
            resolution of inflammation through the PRKAR2A-mediated suppression
            of JAK2/STAT1 signaling.
REFERENCE   5  (residues 1 to 402)
  AUTHORS   Zhou,T., Li,J., Zhao,P., Liu,H., Jia,D., Jia,H., He,L., Cang,Y.,
            Boast,S., Chen,Y.H., Thibault,H., Scherrer-Crosbie,M., Goff,S.P.
            and Li,B.
  TITLE     Palmitoyl acyltransferase Aph2 in cardiac function and the
            development of cardiomyopathy
  JOURNAL   Proc Natl Acad Sci U S A 112 (51), 15666-15671 (2015)
   PUBMED   26644582
REFERENCE   6  (residues 1 to 402)
  AUTHORS   Imaizumi-Scherrer,T., Faust,D.M., Benichou,J.C., Hellio,R. and
            Weiss,M.C.
  TITLE     Accumulation in fetal muscle and localization to the neuromuscular
            junction of cAMP-dependent protein kinase A regulatory and
            catalytic subunits RI alpha and C alpha
  JOURNAL   J Cell Biol 134 (5), 1241-1254 (1996)
   PUBMED   8794865
REFERENCE   7  (residues 1 to 402)
  AUTHORS   Greene,R.M., Lloyd,M.R., Uberti,M., Nugent,P. and Pisano,M.M.
  TITLE     Patterns of cyclic AMP-dependent protein kinase gene expression
            during ontogeny of the murine palate
  JOURNAL   J Cell Physiol 163 (3), 431-440 (1995)
   PUBMED   7775586
REFERENCE   8  (residues 1 to 402)
  AUTHORS   Cadd,G. and McKnight,G.S.
  TITLE     Distinct patterns of cAMP-dependent protein kinase gene expression
            in mouse brain
  JOURNAL   Neuron 3 (1), 71-79 (1989)
   PUBMED   2619996
REFERENCE   9  (residues 1 to 402)
  AUTHORS   Oyen,O., Myklebust,F., Scott,J.D., Hansson,V. and Jahnsen,T.
  TITLE     Human testis cDNA for the regulatory subunit RII alpha of
            cAMP-dependent protein kinase encodes an alternate amino-terminal
            region
  JOURNAL   FEBS Lett 246 (1-2), 57-64 (1989)
   PUBMED   2540040
REFERENCE   10 (residues 1 to 402)
  AUTHORS   Scott,J.D., Glaccum,M.B., Zoller,M.J., Uhler,M.D., Helfman,D.M.,
            McKnight,G.S. and Krebs,E.G.
  TITLE     The molecular cloning of a type II regulatory subunit of the
            cAMP-dependent protein kinase from rat skeletal muscle and mouse
            brain
  JOURNAL   Proc Natl Acad Sci U S A 84 (15), 5192-5196 (1987)
   PUBMED   3037538
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT571271.12 and AC173341.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF533977.1, BC080276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.63 cM"
     Protein         1..402
                     /product="cAMP-dependent protein kinase type II-alpha
                     regulatory subunit"
                     /EC_number="2.7.11.1"
                     /calculated_mol_wt=45458
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine.
                     /evidence=ECO:0000250|UniProtKB:P13861, ECO:0000305;
                     propagated from UniProtKB/Swiss-Prot (P12367.2)"
     Region          4..44
                     /region_name="DD_RIIalpha_PKA"
                     /note="dimerization/docking (D/D) domain of the Type II
                     alpha Regulatory subunit of cAMP-dependent protein kinase;
                     cd12103"
                     /db_xref="CDD:438524"
     Site            order(4..8,10,13..14,17..18,21..22,25,28..31,33..34,
                     37..38,40..41)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:438524"
     Site            order(6,10..11,14..15,18..19,22..23)
                     /site_type="other"
                     /note="AKAP interaction site [polypeptide binding]"
                     /db_xref="CDD:438524"
     Site            48
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P13861; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Region          62..84
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P12367.2)"
     Site            75
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P13861; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Site            77
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P13861; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Site            97
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Region          137..249
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(205..206,215..217)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            213
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by PDPK1.
                     /evidence=ECO:0000250|UniProtKB:P00515; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Site            order(237..239,243..245)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          259..380
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(335..336,345..347)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            348
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P13861; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     Site            order(367..369,373..375)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Site            393
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (P12367.2)"
     CDS             1..402
                     /gene="Prkar2a"
                     /gene_synonym="1110061A24Rik; RII(alpha)"
                     /coded_by="NM_008924.3:315..1523"
                     /db_xref="CCDS:CCDS23536.1"
                     /db_xref="GeneID:19087"
                     /db_xref="MGI:MGI:108025"
ORIGIN      
        1 mshiqipagl tellqgytve vlrqqppdlv dfaveyftrl rearrqesdt fivspttfht
       61 qessavpvie edgesdsdse dadlevpvps kftrrvsvca etfnpdeeee dndprvvhpk
      121 tdeqrcrlqe ackdillfkn ldqeqlsqvl damfekivkt dehvidqgdd gdnfyvierg
      181 tydilvtkdn qtrsvgqydn rgsfgelalm yntpraatii atsegslwgl drvtfrriiv
      241 knnakkrkmf esfiesvplf kslemsermk ivdvigekiy kdgeriiaqg ekadsfyiie
      301 sgevsilirs ktksnknggn qeveiahchk gqyfgelalv tnkpraasay avgdvkclvm
      361 dvqaferllg pcmdimkrni shyeeqlvkm fgsnldlmdp gq
//
