LOCUS       NM_153744               2835 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic
            subunit (Prkag3), mRNA.
ACCESSION   NM_153744
VERSION     NM_153744.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Kido,K., Eskesen,N.O., Henriksen,N.S., Onslev,J., Kristensen,J.M.,
            Larsen,M.R., Hingst,J.R., Knudsen,J.R., Birk,J.B., Andersen,N.R.,
            Jensen,T.E., Pehmoller,C., Wojtaszewski,J.F.P. and Kjobsted,R.
  TITLE     AMPKgamma3 Controls Muscle Glucose Uptake in Recovery From Exercise
            to Recapture Energy Stores
  JOURNAL   Diabetes 72 (10), 1397-1408 (2023)
   PUBMED   37506328
  REMARK    GeneRIF: AMPKgamma3 Controls Muscle Glucose Uptake in Recovery From
            Exercise to Recapture Energy Stores.
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Aoi,W., Inoue,R., Mizushima,K., Honda,A., Bjornholm,M., Takagi,T.
            and Naito,Y.
  TITLE     Exercise-acclimated microbiota improves skeletal muscle metabolism
            via circulating bile acid deconjugation
  JOURNAL   iScience 26 (3), 106251 (2023)
   PUBMED   36915683
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2835)
  AUTHORS   Rhein,P., Desjardins,E.M., Rong,P., Ahwazi,D., Bonhoure,N.,
            Stolte,J., Santos,M.D., Ovens,A.J., Ehrlich,A.M., Sanchez
            Garcia,J.L., Ouyang,Q., Yabut,J.M., Kjolby,M., Membrez,M.,
            Jessen,N., Oakhill,J.S., Treebak,J.T., Maire,P., Scott,J.W.,
            Sanders,M.J., Descombes,P., Chen,S., Steinberg,G.R. and Sakamoto,K.
  TITLE     Compound- and fiber type-selective requirement of AMPKgamma3 for
            insulin-independent glucose uptake in skeletal muscle
  JOURNAL   Mol Metab 51, 101228 (2021)
   PUBMED   33798773
  REMARK    GeneRIF: Compound- and fiber type-selective requirement of
            AMPKgamma3 for insulin-independent glucose uptake in skeletal
            muscle.
REFERENCE   4  (bases 1 to 2835)
  AUTHORS   Cokorinos,E.C., Delmore,J., Reyes,A.R., Albuquerque,B.,
            Kjobsted,R., Jorgensen,N.O., Tran,J.L., Jatkar,A., Cialdea,K.,
            Esquejo,R.M., Meissen,J., Calabrese,M.F., Cordes,J., Moccia,R.,
            Tess,D., Salatto,C.T., Coskran,T.M., Opsahl,A.C., Flynn,D.,
            Blatnik,M., Li,W., Kindt,E., Foretz,M., Viollet,B., Ward,J.,
            Kurumbail,R.G., Kalgutkar,A.S., Wojtaszewski,J.F.P., Cameron,K.O.
            and Miller,R.A.
  TITLE     Activation of Skeletal Muscle AMPK Promotes Glucose Disposal and
            Glucose Lowering in Non-human Primates and Mice
  JOURNAL   Cell Metab 25 (5), 1147-1159 (2017)
   PUBMED   28467931
REFERENCE   5  (bases 1 to 2835)
  AUTHORS   Kuninger,D., Kuzmickas,R., Peng,B., Pintar,J.E. and Rotwein,P.
  TITLE     Gene discovery by microarray: identification of novel genes induced
            during growth factor-mediated muscle cell survival and
            differentiation
  JOURNAL   Genomics 84 (5), 876-889 (2004)
   PUBMED   15475267
REFERENCE   6  (bases 1 to 2835)
  AUTHORS   Barnes,B.R., Marklund,S., Steiler,T.L., Walter,M., Hjalm,G.,
            Amarger,V., Mahlapuu,M., Leng,Y., Johansson,C., Galuska,D.,
            Lindgren,K., Abrink,M., Stapleton,D., Zierath,J.R. and Andersson,L.
  TITLE     The 5'-AMP-activated protein kinase gamma3 isoform has a key role
            in carbohydrate and lipid metabolism in glycolytic skeletal muscle
  JOURNAL   J Biol Chem 279 (37), 38441-38447 (2004)
   PUBMED   15247217
REFERENCE   7  (bases 1 to 2835)
  AUTHORS   Mahlapuu,M., Johansson,C., Lindgren,K., Hjalm,G., Barnes,B.R.,
            Krook,A., Zierath,J.R., Andersson,L. and Marklund,S.
  TITLE     Expression profiling of the gamma-subunit isoforms of AMP-activated
            protein kinase suggests a major role for gamma3 in white skeletal
            muscle
  JOURNAL   Am J Physiol Endocrinol Metab 286 (2), E194-E200 (2004)
   PUBMED   14559719
  REMARK    GeneRIF: the observation that the AMPKgamma3 isoform is expressed
            primarily in white skeletal muscle, in which it is the predominant
            gamma-isoform, strongly suggests that gamma3 has a key role in this
            tissue
REFERENCE   8  (bases 1 to 2835)
  AUTHORS   Yu,H., Fujii,N., Hirshman,M.F., Pomerleau,J.M. and Goodyear,L.J.
  TITLE     Cloning and characterization of mouse 5'-AMP-activated protein
            kinase gamma3 subunit
  JOURNAL   Am J Physiol Cell Physiol 286 (2), C283-C292 (2004)
   PUBMED   14512293
  REMARK    GeneRIF: Gamma3 subunit of AMPK is highly expressed in fast-twitch
            glycolytic skeletal muscle, and wild-type gamma3 functions in the
            regulation of alpha1 catalytic activity, but it is not associated
            with changes in muscle glycogen concentrations
REFERENCE   9  (bases 1 to 2835)
  AUTHORS   Amarger,V., Erlandsson,R., Pielberg,G., Jeon,J.T. and Andersson,L.
  TITLE     Comparative sequence analysis of the PRKAG3 region between human
            and pig: evolution of repetitive sequences and potential new exons
  JOURNAL   Cytogenet Genome Res 102 (1-4), 163-172 (2003)
   PUBMED   14970697
REFERENCE   10 (bases 1 to 2835)
  AUTHORS   Milan,D., Jeon,J.T., Looft,C., Amarger,V., Robic,A., Thelander,M.,
            Rogel-Gaillard,C., Paul,S., Iannuccelli,N., Rask,L., Ronne,H.,
            Lundstrom,K., Reinsch,N., Gellin,J., Kalm,E., Roy,P.L., Chardon,P.
            and Andersson,L.
  TITLE     A mutation in PRKAG3 associated with excess glycogen content in pig
            skeletal muscle
  JOURNAL   Science 288 (5469), 1248-1251 (2000)
   PUBMED   10818001
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK036585.1, AC152409.8 and BX637099.1.
            
            [WARNING] On Jul 30, 2024 this sequence was replaced by
            NM_153744.4.
            
            On Jun 2, 2009 this sequence version replaced NM_153744.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036585.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate          :: PMID: 14512293, 14559719, 15247217
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2255              AK036585.1         2-2256
            2256-2549           AC152409.8         70389-70682
            2550-2835           BX637099.1         1-286               c
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.54 cM"
     gene            1..2835
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /note="protein kinase, AMP-activated, gamma 3
                     non-catalytic subunit"
                     /db_xref="GeneID:241113"
                     /db_xref="MGI:MGI:1891343"
     exon            1..138
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..1575
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /note="AMP-activated protein kinase gamma subunit; AMPK
                     gamma3; AMPK subunit gamma-3; protein kinase,
                     AMP-activated, gamma 3 non-catatlytic subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit gamma-3"
                     /protein_id="NP_714966.1"
                     /db_xref="CCDS:CCDS15055.1"
                     /db_xref="GeneID:241113"
                     /db_xref="MGI:MGI:1891343"
                     /translation="MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATS
                     SERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAV
                     PLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPG
                     PSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFA
                     MVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIY
                     LQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIF
                     GALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQV
                     VGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIARE
                     QVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA"
     exon            139..178
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            179..331
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            332..738
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            739..820
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            821..879
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            880..925
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            926..980
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1107
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1273
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1311
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1458
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1693
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..2832
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2514
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /note="major polyA site"
     regulatory      2814..2819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /note="hexamer: AAGAAA"
     polyA_site      2832
                     /gene="Prkag3"
                     /gene_synonym="Amkg3; Ampkg3; AMPKg3L; AMPKg3S"
                     /note="major polyA site"
ORIGIN      
        1 agagagaacc cgatctctca agggcctgcc gccagggacc agcccagaat agtgccaccc
       61 tgccgccgcc tgtcgcgcac ttgctaacgg actagagctg gccgcatgga gcccgagctg
      121 gagcacacat tgcctgggac cctgacctgg agccacagtg ggggtccaga gagtcaagag
      181 atggacttct tagaacaagg agaaaactca tggccctcac cagctgtggc caccagctca
      241 gaaagaacct gtgccatacg gggagtcaag gcttccagat ggacgagaca ggaggccgta
      301 gaggaagcag aaccaccagg tttgggagaa ggtgcccagt ccagaccagc tgctgagtcc
      361 accaggcagg aggccacatt cccgaaggcc acacccttgg ctcaagctgt tcccttggct
      421 gaagcggaga cctcccccac agggtgggac ctgctcttgc ccgactgtgc agcctcagca
      481 gggggctcca gcacaggtga cctggagctg accatagagt tcccagcccc agaggcctgg
      541 gactgtgagc tggaaggcct ggggaaggac aggcctcgtc ctggtccatc cccacaggcc
      601 ccacttctcg gcctgagttg ggatgacgaa cttcagaagc ccggagccca ggtctacatg
      661 cacttcatgc aggaacacac ctgttatgat gccatggcta ccagctccaa attggtcatc
      721 tttgacacca cgttggagat aaagaaggct ttctttgcca tggtggccaa cggtgtgagg
      781 gcagctcctc tgtgggacag caagaagcag agctttgtgg gtatgctcac catcactgac
      841 tttatcctgg tgctgcaccg gtactacaga tcccccctgg tccagatcta tgagattgaa
      901 gaacataaga ttgagacctg gagggagatc tacctacaag gctgcttcaa gcctctagtc
      961 tccatctctc ccaatgacag cctgtttgaa gctgtctatg ccctcatcaa gaaccgaatc
     1021 caccgcctgc cggtcctgga cccggtctct ggcactgtgc tctacatact cacacacaag
     1081 cggctactca agttcctgca tatatttggt gccctgttgc cccggccctc cttcctctgc
     1141 cgcactatcc aagacttggg catcggcaca ttccgagatt tggctgtagt tctggaaaca
     1201 gctcctgtcc tgactgcgct ggacatcttt gtggaccgac gtgtgtctgc actgcctgtg
     1261 gtcaatgaat ctggtcaggt cgtgggcctc tactcccgct ttgatgtcat tcacctggct
     1321 gcccagcaaa cctacaacca cctagacatg agtgtgggag aagctctgag acagaggaca
     1381 ctgtgtctgg agggagttct ctcctgccag ccccacgaga gcctaggtga agtcattgac
     1441 aggatcgcac gggaacaggt gcataggctg gtgttggtgg atgagaccca gcatcttctg
     1501 ggcgtggtct ccctctctga catacttcaa gcactggtac tcagccctgc tggcatcgat
     1561 gccctcagcg cctgagacct gagtcctcac acccaagcca tcttcttcac ctgaaaacca
     1621 acgaaaggat cttggaactt gaaggggaat tggccttcat cttctctcac ccccatctgc
     1681 tggctctgct ctggctctcc agccttccct gattgtcctt gctacctata ctctcagaag
     1741 cctttgggca tgcccagtgc accaggacga tgaaagaaca acagagtcaa gtctgcaagt
     1801 ccctgaacca gagatcctgc agtcagtcgt ggatcatctg tgtgtcctgt ccctgcttac
     1861 tcttttccct gctcctggat cagggctgag ctcagtgggg cagtgagcct gcggcctatg
     1921 gatttagata ggggcctgaa atgcttggtt tctggaccag gtacacttgt gttccccgct
     1981 cccgggagcc ctggctgttc tccccctgtg ggagccacca ttgcatcacc atcatccacc
     2041 agcttactct cctggtgctt ccaagatgct tgatgccaac actgaacagc ccttgatcct
     2101 agagctgtct gctggagtgg gggtgcctga tactgttgca tgggaggatg tgaggggagg
     2161 ctccttagct ctcaccagtg gacattgttc catcccctca gtctcttccc atcagacaga
     2221 gggtcttata ttcagatcct taagaatgaa caatcaagaa aagaaagaga gaggagagag
     2281 agagagagag agaagagaga gagagaaagg aaggaaggaa ggaaggaagg aaggaagaaa
     2341 gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga aagaaaggag
     2401 caagccccaa tcttggatgc tataggcttg ctcaactgct acagtcattg agatgaactt
     2461 gtgccaaact ccttctgtct cctgaattca aagacttgga gggaaaagct gcaagtggcc
     2521 tttctttttt gtgcatagaa tgttattccg ccaacaactt tttttaatct ttgatttgta
     2581 acaaaatgat aaggagagtg cttattaatt aattactcac acaggaggtg aaacccagtc
     2641 tacaaatggg agtctgcagg aaacaccact gatcctgtaa cattggatgt aatttctacc
     2701 atgaccccag ttcaccaggt agaaagaaat taatctcagt caattctggg tctcttcatc
     2761 tgcctttacc ctaccctgtg atgacagggg ccctcagatc cagtggtcca atgaagaaag
     2821 agtggattca taaaa
//
