LOCUS       NM_145401               3340 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus protein kinase, AMP-activated, gamma 2 non-catalytic
            subunit (Prkag2), transcript variant 1, mRNA.
ACCESSION   NM_145401
VERSION     NM_145401.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3340)
  AUTHORS   Martinez-Sanchez,A., Nguyen-Tu,M.S., Cebola,I., Yavari,A.,
            Marchetti,P., Piemonti,L., de Koning,E., Shapiro,A.M.J.,
            Johnson,P., Sakamoto,K., Smith,D.M., Leclerc,I., Ashrafian,H.,
            Ferrer,J. and Rutter,G.A.
  TITLE     MiR-184 expression is regulated by AMPK in pancreatic islets
  JOURNAL   FASEB J 32 (5), 2587-2600 (2018)
   PUBMED   29269398
REFERENCE   4  (bases 1 to 3340)
  AUTHORS   Yavari,A., Bellahcene,M., Bucchi,A., Sirenko,S., Pinter,K.,
            Herring,N., Jung,J.J., Tarasov,K.V., Sharpe,E.J., Wolfien,M.,
            Czibik,G., Steeples,V., Ghaffari,S., Nguyen,C., Stockenhuber,A.,
            Clair,J.R.S., Rimmbach,C., Okamoto,Y., Yang,D., Wang,M.,
            Ziman,B.D., Moen,J.M., Riordon,D.R., Ramirez,C., Paina,M., Lee,J.,
            Zhang,J., Ahmet,I., Matt,M.G., Tarasova,Y.S., Baban,D., Sahgal,N.,
            Lockstone,H., Puliyadi,R., de Bono,J., Siggs,O.M., Gomes,J.,
            Muskett,H., Maguire,M.L., Beglov,Y., Kelly,M., Dos Santos,P.P.N.,
            Bright,N.J., Woods,A., Gehmlich,K., Isackson,H., Douglas,G.,
            Ferguson,D.J.P., Schneider,J.E., Tinker,A., Wolkenhauer,O.,
            Channon,K.M., Cornall,R.J., Sternick,E.B., Paterson,D.J.,
            Redwood,C.S., Carling,D., Proenza,C., David,R., Baruscotti,M.,
            DiFrancesco,D., Lakatta,E.G., Watkins,H. and Ashrafian,H.
  TITLE     Mammalian gamma2 AMPK regulates intrinsic heart rate
  JOURNAL   Nat Commun 8 (1), 1258 (2017)
   PUBMED   29097735
  REMARK    GeneRIF: gamma2 AMPK activation downregulates fundamental
            sinoatrial cell pacemaker mechanisms to lower heart rate, including
            sarcolemmal hyperpolarization-activated current (I f) and ryanodine
            receptor-derived diastolic local subsarcolemmal Ca(2+) release. In
            contrast, loss of gamma2 AMPK induces a reciprocal phenotype of
            increased heart rate, and prevents the adaptive intrinsic
            bradycardia of endurance training.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3340)
  AUTHORS   Cao,Y., Bojjireddy,N., Kim,M., Li,T., Zhai,P., Nagarajan,N.,
            Sadoshima,J., Palmiter,R.D. and Tian,R.
  TITLE     Activation of gamma2-AMPK Suppresses Ribosome Biogenesis and
            Protects Against Myocardial Ischemia/Reperfusion Injury
  JOURNAL   Circ Res 121 (10), 1182-1191 (2017)
   PUBMED   28835357
  REMARK    GeneRIF: Increased AMPK-gamma2 activation is required to protect
            against myocardial ischemia/reperfusion injury.
REFERENCE   6  (bases 1 to 3340)
  AUTHORS   Banerjee,S.K., Ramani,R., Saba,S., Rager,J., Tian,R., Mathier,M.A.
            and Ahmad,F.
  TITLE     A PRKAG2 mutation causes biphasic changes in myocardial AMPK
            activity and does not protect against ischemia
  JOURNAL   Biochem Biophys Res Commun 360 (2), 381-387 (2007)
   PUBMED   17597581
  REMARK    GeneRIF: Despite high glycogen content, the TGT400N heart is not
            protected against ischemia-reperfusion injury.
REFERENCE   7  (bases 1 to 3340)
  AUTHORS   Al-Regaiey,K.A., Masternak,M.M., Bonkowski,M.S., Panici,J.A.,
            Kopchick,J.J. and Bartke,A.
  TITLE     Effects of caloric restriction and growth hormone resistance on
            insulin-related intermediates in the skeletal muscle
  JOURNAL   J Gerontol A Biol Sci Med Sci 62 (1), 18-26 (2007)
   PUBMED   17301033
  REMARK    GeneRIF: CR resulted in incerasd AMPK phosphorylation and, hence,
            activation. GHR knockout animals also exhibited invrease p-AMPK
            levels. P-AMPK levels were not altered by disruption of the GHR
            gene.
REFERENCE   8  (bases 1 to 3340)
  AUTHORS   Steinberg,G.R., Watt,M.J., Fam,B.C., Proietto,J., Andrikopoulos,S.,
            Allen,A.M., Febbraio,M.A. and Kemp,B.E.
  TITLE     Ciliary neurotrophic factor suppresses hypothalamic AMP-kinase
            signaling in leptin-resistant obese mice
  JOURNAL   Endocrinology 147 (8), 3906-3914 (2006)
   PUBMED   16675525
  REMARK    GeneRIF: CNTF(Ax15) bypasses diet-induced leptin resistance to
            reduce hypothalamic AMPK activity
REFERENCE   9  (bases 1 to 3340)
  AUTHORS   Davies,J.K., Wells,D.J., Liu,K., Whitrow,H.R., Daniel,T.D.,
            Grignani,R., Lygate,C.A., Schneider,J.E., Noel,G., Watkins,H. and
            Carling,D.
  TITLE     Characterization of the role of gamma2 R531G mutation in
            AMP-activated protein kinase in cardiac hypertrophy and
            Wolff-Parkinson-White syndrome
  JOURNAL   Am J Physiol Heart Circ Physiol 290 (5), H1942-H1951 (2006)
   PUBMED   16339829
REFERENCE   10 (bases 1 to 3340)
  AUTHORS   Turnley,A.M., Stapleton,D., Mann,R.J., Witters,L.A., Kemp,B.E. and
            Bartlett,P.F.
  TITLE     Cellular distribution and developmental expression of AMP-activated
            protein kinase isoforms in mouse central nervous system
  JOURNAL   J Neurochem 72 (4), 1707-1716 (1999)
   PUBMED   10098881
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116151.10 and AC125270.7.
            
            On Jan 1, 2010 this sequence version replaced NM_145401.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154296.1, BC015283.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-632               AC116151.10        129615-130246       c
            633-704             AC116151.10        58069-58140         c
            705-984             AC116151.10        51396-51675         c
            985-1199            AC125270.7         142343-142557       c
            1200-1269           AC125270.7         103242-103311       c
            1270-1373           AC125270.7         84001-84104         c
            1374-1455           AC125270.7         75603-75684         c
            1456-1514           AC125270.7         73624-73682         c
            1515-1560           AC125270.7         71908-71953         c
            1561-1615           AC125270.7         70080-70134         c
            1616-1742           AC125270.7         68803-68929         c
            1743-1908           AC125270.7         66413-66578         c
            1909-1946           AC125270.7         65974-66011         c
            1947-2093           AC125270.7         64050-64196         c
            2094-2187           AC125270.7         60998-61091         c
            2188-3340           AC125270.7         57654-58806         c
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 11.93 cM"
     gene            1..3340
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="protein kinase, AMP-activated, gamma 2
                     non-catalytic subunit"
                     /db_xref="GeneID:108099"
                     /db_xref="MGI:MGI:1336153"
     exon            1..632
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     CDS             519..2219
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     5'-AMP-activated protein kinase subunit gamma-2; AMPK
                     gamma-2 chain; AMPK subunit gamma-2"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit gamma-2
                     isoform 1"
                     /protein_id="NP_663376.2"
                     /db_xref="CCDS:CCDS19130.1"
                     /db_xref="GeneID:108099"
                     /db_xref="MGI:MGI:1336153"
                     /translation="MGSAAMDTKKKKEVSSPGGSSGKKNPSLKRRSLRVHIPDLSSFA
                     MPLLDGDVENSEKHSSRKVDSPFSSGSPSRGLFSRGPQPRPSSPVSAPVRPKTSPGSP
                     KTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPSTSPGGIRFFSRSRKTSSVS
                     SSPSTPTQVTKQHPFPLESYKQEPERPESRIYASSSPPDTGQRFCLAFQSPARPPLAS
                     PTYHAPLRTAVLAAAPGPAEAGMLEKLEFQEEEDSESGVYMRFMRSHKCYDIVPTSSK
                     LVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQ
                     IYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNA
                     LYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFV
                     ERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKC
                     SKLETLETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
                     "
     exon            633..704
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            705..984
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1199
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1269
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1373
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1455
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1514
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1560
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1615
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1742
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1908
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..1946
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2093
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2187
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..3340
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2464..2469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="hexamer: ATTAAA"
     polyA_site      2487
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="major polyA site"
     regulatory      3304..3309
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="hexamer: AATAAA"
     polyA_site      3321
                     /gene="Prkag2"
                     /gene_synonym="2410051C13Rik; AAKG; AAKG2; H91620p; WPWS"
                     /note="major polyA site"
ORIGIN      
        1 ccctgtcgct ttattgtgcg gcccagggtt atgcatttct ctaccaagtg tctctggcca
       61 ccgtttccat cgaggcacag acacgagaag gctgttttcc aaattgctgt tgttaattgc
      121 acctgcctgg ccgactacct ctgggaattt gtgggaggag ccgagaggtg gaaaatgttg
      181 cttcgctttg cagaaggagg aaaactttat cacccagcac aagacttctg ccacgcgtaa
      241 ccaaaagtag cacgggccga gactctgcac ccaatgattc cgtaggagaa agaggacgct
      301 ccaacccggc attccgcgga ctgccagccc ctcgggtgga gccccgcgtg ttacctgcgc
      361 cacccgccct ccccgcggaa gtccccacga ggtgttttca gggtgggagg acagtcataa
      421 aaagcgcgtg gctccagtgt aacggcagca gtcctgggag gcagcgaggg gaccgaaccc
      481 cggggacgag gcaccggaat taacttctag ctaaagttat gggaagcgca gccatggaca
      541 ccaagaagaa gaaagaggtt tccagcccgg gcgggagcag tggcaaaaag aaccctagcc
      601 tgaagaggcg gtcactgcga gtgcacattc cggacctgag ttcctttgcc atgccgctcc
      661 tggatggaga tgtggagaat tcagaaaagc attcatctcg gaaggtggac agtcccttca
      721 gctcaggcag cccctccaga ggactcttct ccagaggccc ccagccccgg ccctccagcc
      781 ctgtgtctgc tcccgtgagg cccaagacga gccctggctc tcccaaaact gtgttcccgt
      841 tctcctacca ggagtcccct ccacgctctc cacgccggat gagcttcagt gggatcttcc
      901 gctcctcatc caaagagtct tcgcccaact ccaacccgtc tacctctcct gggggcatcc
      961 ggttcttctc acggtccaga aaaacctcca gcgtctcctc ttctccgtca acacccaccc
     1021 aagtgaccaa gcagcaccca tttcccctgg agtcctacaa gcaggagcct gaacgcccag
     1081 agagccgcat ctatgcttcc tcgtcccctc cagacactgg gcagcgcttc tgcctggcct
     1141 ttcagagccc agcgcgacct ccgctggcat ccccgacata ccatgctcct ttgagaactg
     1201 cggtgctggc ggcggccccg ggacccgcgg aagccggcat gctggagaag ctggagttcc
     1261 aggaggaaga agactcagaa agtggcgttt atatgcgatt catgaggtca cacaagtgtt
     1321 atgacatcgt tccaaccagt tcaaagcttg ttgtcttcga cactacgttg caagtcaaaa
     1381 aggccttctt tgccttggta gccaacggag tccgtgcagc gccgctgtgg gaaagtaaaa
     1441 agcagagctt tgtaggaatg ctcacgatta cagatttcat aaatatccta cacagatact
     1501 ataaatcacc catggtacag atttatgaat tggaagaaca taagattgaa acgtggaggg
     1561 agttgtactt acaagaaacc ttcaagcctt tggtgaacat ctctccagat gcgagcctct
     1621 tcgatgctgt atactcgttg atcaaaaata aaatccacag attgccagtt attgacccta
     1681 tcagtgggaa cgcactttat atacttaccc acaaaagaat cctcaagttc ctccagcttt
     1741 ttatgtctga catgccaaag cctgccttca tgaagcagaa cctggatgag cttggaatcg
     1801 gaacgtacca caatattgcc ttcattcacc cagacactcc catcatcaaa gccttgaaca
     1861 tcttcgtgga gagacggata tccgcattgc ctgtggtgga tgagtcagga aaagttgtag
     1921 atatttattc caagtttgat gtaattaatc ttgctgctga gaaaacgtac aataacttgg
     1981 acatcacagt gacccaggcc ctgcagcacc gctcgcagta ttttgagggt gtggtgaagt
     2041 gcagtaagct ggaaacactg gagaccatcg tggacaggat agtgagagcc gaggtccatc
     2101 ggctggttgt agtgaatgaa gcagatagca ttgtgggtat tatctccctg tcagacatcc
     2161 tgcaagccct gatcctcaca ccagcaggtg ccaaacagaa ggagacagag actgaatgac
     2221 cgccgtgaat gtagacgccc gcgcaggaga acttgaacaa agtctcgggg tcacggtttg
     2281 cctcatgaac actggctgca atagaagaga gtaaaatggc taagaatgta taccagggtt
     2341 tagcgatggg tatttttcca gtgatttgta agtataggaa aagattttat gtgcttgaag
     2401 attcaggctt gcgttcaaag actgccctca gacctgtctg aaggattttc aatgctgtat
     2461 gtcattaaag tgcactgtgt cctgaaatag tactactttt catttcaaag aagtcactgg
     2521 gatggaacag gtgacagaaa gtgagtgaac ggcacgtcca gatcacggtg ccttatgtcc
     2581 aaacgcaata tgtcaccgcc gctgccgcac gccccaaagt gctgctgaag agacacccag
     2641 cccaagtgtg agcatcttca gaccttttac ccagtctctt ccttttcact taattggttg
     2701 aaaagtttta atttgagtac aggcaactga aaatgtgatg tgatgcctct aagcgctctg
     2761 catccgtcct gttctggctg ccgtgttagc gaggccggct tggcaacagc gctttattta
     2821 tttttactct catgcaggtt tcaacctaca tcctttggtg agtagacgtc accataccta
     2881 cgaataaaac cttgattgtt ctctcaagtg gaaaatctct ggagatttta gtttggatct
     2941 ggaaaggcta tgccttccaa acagccactg tagggtcttc taaacgttta tgaaaacagg
     3001 ctgtggtcgc agggaagaag taacggaaga cgtctttttt tttattgtgt gttgaaatgt
     3061 aaactcatga tgtttgtgtg accgctgaca agacgcttgt aacttttatt gtgacacata
     3121 aaacagtatt gtcatgcagt tgaagggaaa ctctttcccc tcccacaccc cacaaccccc
     3181 acagccccca gtgtcagtgc tctgaaaatg ttgacactat atatctgtgt gtgtatatat
     3241 gagcagagtt tgggggtttg ttttggggtt ttgtttcgtt ttgtttttca gattgaaaat
     3301 taaaataaat cctgtctacc agtttgctaa agtttttatg
//
