LOCUS       NM_178143               8201 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus protein kinase, AMP-activated, alpha 2 catalytic
            subunit (Prkaa2), transcript variant 1, mRNA.
ACCESSION   NM_178143 XM_131633
VERSION     NM_178143.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Hossain,M.N., Gao,Y., Li,X., Zhao,L., Liu,X., Marie de Avila,J.,
            Zhu,M.J. and Du,M.
  TITLE     Single-cell RNA transcriptomics in mice reveals embryonic origin of
            fibrosis due to maternal obesity
  JOURNAL   EBioMedicine 109, 105421 (2024)
   PUBMED   39476533
  REMARK    Erratum:[EBioMedicine. 2025 Jan 27;112:105573. doi:
            10.1016/j.ebiom.2025.105573.. PMID: 39874704]
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Li,M., Wang,Y., Wei,X., Cai,W.F., Wu,J., Zhu,M., Wang,Y., Liu,Y.H.,
            Xiong,J., Qu,Q., Chen,Y., Tian,X., Yao,L., Xie,R., Li,X., Chen,S.,
            Huang,X., Zhang,C., Xie,C., Wu,Y., Xu,Z., Zhang,B., Jiang,B.,
            Wang,Z.C., Li,Q., Li,G., Lin,S.Y., Yu,L., Piao,H.L., Deng,X.,
            Han,J., Zhang,C.S. and Lin,S.C.
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to
            glutamine
  JOURNAL   Cell Res 34 (10), 683-706 (2024)
   PUBMED   38898113
REFERENCE   3  (bases 1 to 8201)
  AUTHORS   Ma,H., Jia,H., Zou,W., Ji,F., Wang,W., Zhao,J., Yuan,C. and Jiao,J.
  TITLE     Gasdermin D Mediated Mitochondrial Metabolism Orchestrate
            Neurogenesis Through LDHA During Embryonic Development
  JOURNAL   Adv Sci (Weinh) 11 (35), e2402285 (2024)
   PUBMED   39033542
REFERENCE   4  (bases 1 to 8201)
  AUTHORS   Li,Y., Munoz-Mayorga,D., Nie,Y., Kang,N., Tao,Y., Lagerwall,J.,
            Pernaci,C., Curtin,G., Coufal,N.G., Mertens,J., Shi,L. and Chen,X.
  TITLE     Microglial lipid droplet accumulation in tauopathy brain is
            regulated by neuronal AMPK
  JOURNAL   Cell Metab 36 (6), 1351-1370 (2024)
   PUBMED   38657612
REFERENCE   5  (bases 1 to 8201)
  AUTHORS   Hu,L.L., Liao,M.H., Liu,Y.X., Xing,C.H., Nong,L.L., Yang,F.L. and
            Sun,S.C.
  TITLE     Loss of AMPK activity induces organelle dysfunction and oxidative
            stress during oocyte aging
  JOURNAL   Biol Direct 19 (1), 29 (2024)
   PUBMED   38654312
  REMARK    GeneRIF: Loss of AMPK activity induces organelle dysfunction and
            oxidative stress during oocyte aging.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8201)
  AUTHORS   Viollet,B., Andreelli,F., Jorgensen,S.B., Perrin,C., Geloen,A.,
            Flamez,D., Mu,J., Lenzner,C., Baud,O., Bennoun,M., Gomas,E.,
            Nicolas,G., Wojtaszewski,J.F., Kahn,A., Carling,D., Schuit,F.C.,
            Birnbaum,M.J., Richter,E.A., Burcelin,R. and Vaulont,S.
  TITLE     The AMP-activated protein kinase alpha2 catalytic subunit controls
            whole-body insulin sensitivity
  JOURNAL   J Clin Invest 111 (1), 91-98 (2003)
   PUBMED   12511592
  REMARK    GeneRIF: extramuscular AMPKalpha2 catalytic subunit has a role in
            whole-body insulin action in vivo, probably through modulation of
            sympathetic nervous activity
REFERENCE   7  (bases 1 to 8201)
  AUTHORS   Zong,H., Ren,J.M., Young,L.H., Pypaert,M., Mu,J., Birnbaum,M.J. and
            Shulman,G.I.
  TITLE     AMP kinase is required for mitochondrial biogenesis in skeletal
            muscle in response to chronic energy deprivation
  JOURNAL   Proc Natl Acad Sci U S A 99 (25), 15983-15987 (2002)
   PUBMED   12444247
  REMARK    GeneRIF: By sensing the energy status of the muscle cell, AMPK is a
            critical regulator involved in initiating mitochondrial biogenesis.
REFERENCE   8  (bases 1 to 8201)
  AUTHORS   Tannour-Louet,M., Porteu,A., Vaulont,S., Kahn,A. and
            Vasseur-Cognet,M.
  TITLE     A tamoxifen-inducible chimeric Cre recombinase specifically
            effective in the fetal and adult mouse liver
  JOURNAL   Hepatology 35 (5), 1072-1081 (2002)
   PUBMED   11981757
REFERENCE   9  (bases 1 to 8201)
  AUTHORS   Turnley,A.M., Stapleton,D., Mann,R.J., Witters,L.A., Kemp,B.E. and
            Bartlett,P.F.
  TITLE     Cellular distribution and developmental expression of AMP-activated
            protein kinase isoforms in mouse central nervous system
  JOURNAL   J Neurochem 72 (4), 1707-1716 (1999)
   PUBMED   10098881
REFERENCE   10 (bases 1 to 8201)
  AUTHORS   Salt,I., Celler,J.W., Hawley,S.A., Prescott,A., Woods,A.,
            Carling,D. and Hardie,D.G.
  TITLE     AMP-activated protein kinase: greater AMP dependence, and
            preferential nuclear localization, of complexes containing the
            alpha2 isoform
  JOURNAL   Biochem J 334 (Pt 1) (Pt 1), 177-187 (1998)
   PUBMED   9693118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627307.18, BC138565.1 and AL929466.8.
            
            On Apr 24, 2009 this sequence version replaced NM_178143.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.6528.1, AK134573.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AL627307.18        20853-20939         c
            88-2079             BC138565.1         97-2088
            2080-8201           AL929466.8         55048-61169         c
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 49.05 cM"
     gene            1..8201
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /note="protein kinase, AMP-activated, alpha 2 catalytic
                     subunit"
                     /db_xref="GeneID:108079"
                     /db_xref="MGI:MGI:1336173"
     exon            1..194
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1759
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.31"
                     /note="isoform 1 is encoded by transcript variant 1;
                     5'-AMP-activated protein kinase catalytic subunit alpha-2;
                     AMPK subunit alpha-2; ACACA kinase; HMGCR kinase;
                     acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA
                     reductase kinase"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase catalytic
                     subunit alpha-2 isoform 1"
                     /protein_id="NP_835279.2"
                     /db_xref="CCDS:CCDS18416.1"
                     /db_xref="GeneID:108079"
                     /db_xref="MGI:MGI:1336173"
                     /translation="MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAV
                     KILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELF
                     DYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAQMNAKIADFGL
                     SNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEH
                     VPTLFKKIRGGVFYIPDYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYL
                     FPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIM
                     NQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKP
                     KSLAVKKAKWHLGIRSQSKACDIMAEVYRAMKQLGFEWKVVNAYHLRVRRKNPVTGNY
                     VKMSLQLYLVDSRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRARSSFDSSTAE
                     NHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR"
     exon            195..336
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            337..430
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            431..575
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            576..663
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            664..888
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1393
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1520
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..8201
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7958..7963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /note="hexamer: AATAAA"
     polyA_site      7977
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /note="major polyA site"
     regulatory      8008..8013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
                     /note="hexamer: AATACA"
     polyA_site      8038
                     /gene="Prkaa2"
                     /gene_synonym="2310008I11Rik; A830082D05; AMPKalpha2"
ORIGIN      
        1 gggtaggcgg cggcggcggc ggtggcggcg gtagcggcgg cggcagcggt agcggcggcg
       61 gcgctcagag cccgcggcag ggggagcccg tgcgccgaac atggctgaga agcagaagca
      121 cgacgggcgg gtgaagatcg gacactacgt cctgggggac accctgggcg tcggcacctt
      181 cggcaaagtg aagattggag aacaccaatt gacaggccat aaagtggcag ttaagatctt
      241 aaatagacag aagattcgca gtttagatgt tgttggaaaa ataaaacgag aaattcaaaa
      301 tcttaaactc tttcgtcatc ctcatattat caaactctac caggtgatca gcactccgac
      361 agactttttt atggtaatgg aatatgtgtc tggaggtgaa ttgttcgact acatctgcaa
      421 acatgggcgg gttgaagagg tggaagcgcg ccggctcttc cagcagatcc tgtctgccgt
      481 ggattactgt cacaggcata tggttgtcca tagggacctg aagccagaga atgtgctgct
      541 ggatgcccag atgaacgcta agatagctga ctttggacta tctaatatga tgtcagatgg
      601 tgaatttcta cgaactagct gtggatcgcc aaattatgca gcacctgagg tcatctcagg
      661 aaggctgtat gcaggtcccg aggtcgatat ctggagctgt ggtgtcatcc tgtatgccct
      721 tctctgtggc accctccctt tcgatgatga gcacgtgcct acgctcttca agaagatccg
      781 agggggtgtg ttttacatcc cagactatct caaccgttct gtcgccactc tgctgatgca
      841 catgctccag gtggaccccc tgaagcgagc gactatcaaa gacatacgag aacatgaatg
      901 gtttaaacag gatttgccca gctacctatt tcctgaagac ccctcctacg atgcgaatgt
      961 cattgacgat gaggctgtga aggaagtctg tgagaaattc gagtgtacag agtcagaagt
     1021 gatgaatagt ctgtatagtg gtgaccctca agaccagctt gcagtggctt atcatcttat
     1081 cattgacaat cggagaataa tgaaccaagc cagtgagttc tacctcgcct ctagtcctcc
     1141 atcaggttct tttatggatg acagcgccat gcatattcct ccaggcttga aaccacatcc
     1201 agaaaggatg ccgcctctca tcgcagacag ccccaaagca cgctgtccac tggatgcact
     1261 caacacaacg aagcccaagt ctctagctgt gaaaaaagcc aagtggcatc ttggaatccg
     1321 aagccagagc aaagcgtgtg acattatggc tgaagtgtac cgagctatga agcagctggg
     1381 ttttgaatgg aaggtagtga atgcatacca tcttcgagta agaagaaaaa acccagtgac
     1441 tggcaactat gtgaaaatga gcttacagct ttacctggta gacagtcgga gctatcttct
     1501 ggacttcaaa agcatcgatg atgaggtggt ggagcagagg tctggttctt caacacccca
     1561 gcgctcctgt tctgctgcgg gcctccacag agcacggtca agttttgatt ccagcacagc
     1621 tgagaaccac tccctttctg gctctctcac tggctctttg actggcagca ctttgtcctc
     1681 ggcatccccg cgcctgggca gtcacaccat ggattttttt gaaatgtgcg ccagtcttat
     1741 cactgcttta gctcgttgat tatccacccc tggtctctgt ctttctgtca cccccctgtg
     1801 aaatcacata cactctttaa attattatct cactcggtat tacgtgctct gcaatagaag
     1861 ttggtgtgaa cattcccagg tgacatgcag tgttattgaa atcaccgaaa acacagaaat
     1921 ccagcattct gtttactcgt agaactctgt aactctcctg tgcctgtgac aagtttgcat
     1981 gggtagtact gggtgagtgt ggtggcgctt gctaacttac acctgtgaat tcgctgtaga
     2041 tggcaagcac acctcactga tgaacgaacc cgctgaggta ggggtggttc aatgggcctc
     2101 cttccttcat gtttcctgta cgtaaatccg gtttacctcc taaacttcag cagctgttag
     2161 gctgcctggg cactcttgca caagaagatt aagatataga ataactgtta gggaaatatt
     2221 atttcaatgt agaaatctga aaatcctgtc cccctaaata ttagaaacca aagtctttta
     2281 aattatttct gcaaatactg cctagtatta gccattaaga ctgtgtttct gataacagga
     2341 gcctaatctc ccagcttcct gacagttgtg caaggcgcct agttaaatca caccaccacc
     2401 aagcaaaaca aaaccatgca aaattagtac acagttcaat gagacaagca aaataccaac
     2461 aacagtctct taaaagaaaa ttctactcta tgatcttgtt tgtgtttatc agactggggt
     2521 agctgggagg agggccggca gtcatggccc tctggcatgt ttgctaattc ccttaacatt
     2581 tgttatcgat gccacctcct ccctccgtct tcctcaggag cctctgtctt gctcaatgct
     2641 tttccctttc attaatcctt tagctaaaaa ccttgctttg gccatcatgt ctttgtatac
     2701 ttccaaagct tgatctctgt gaccttcact gttgaacctg attggacagg gaagccttaa
     2761 atatttaaaa gtatattctc ctggaatacg tatatgtgtt gtttacatat atatacatat
     2821 gtattgacac atcagtgctt ttaatcaaaa aaaaaccaaa catttttgtg caagtttttt
     2881 ttttaattta agacaaaaaa gcactatatt gcaagataga ttaagttaat tgattatatg
     2941 ttgttttggt aaaagtattt taaattatgt gtaatttatc taataattat ttattcatct
     3001 attgtattta tgttgtaatt ttaaaaatca ttgatctgat tcaccctaga aaatattaag
     3061 ctcttgaaaa aaaaaaaaag caacattgcc ttgacaggtt tctcttcacc aaaaattgta
     3121 tattttctat ttaccaagaa gcaaatgaga ctgttagacc aggtttctca tgagattcca
     3181 gaagacaagt ttgtctgtct ttaaatagct ttttgaatca ggaatttgca tcatatcaca
     3241 tcagataact ttgtagttct tttaaaaaga tggtcaatat ttatcactcc aacttctcaa
     3301 tggtaaaata aaagaatctt tgcccactga ttgagaagga gagtgtataa tttaaggtac
     3361 cttaagttta atctttgaaa gattgccatc ttgtggcaat agatggtaat tttagtagtt
     3421 attttaatac caatacttag tcctgcctgg gttatctaat gtgttttatg aatgtggcat
     3481 agttctgact tctccaaatg tgtctgtatc cttgtttgtg gggtggggga gagatcacct
     3541 tatgatgttt gcctaatcat ggcatggaag gccttgaagt aaggaactga atagattctt
     3601 tacctctaat aacagcatcc acaacagtga tgaaggcagg aaggaggcag aatgctttta
     3661 gtctagtgaa tttaaaccaa aattacttta tatgaatgat tatgatgcta ttcaagagct
     3721 aaaagttatt tactattgtc ttaattcatt ccagtattat gaatacccag gttgacagtt
     3781 acctgctgaa tttcaggaga actgaaggtc attttgtttc ttttaacatt ttaagacata
     3841 attatttaat gaatctagat gatagagtaa gttggttttc agagagaatt cagtgactca
     3901 tctcgaaaag cagaggcagg aattcagtca cagcataggg atttgttgct cttggaaaca
     3961 gagaaggaaa gaattctttg caaatttaca gttcctacat ttcagtcaat cctatgggta
     4021 tggtaggtac tgagtttaga gcagcctatc ctcttggatc gtgcggagaa taactattaa
     4081 gttatagatg tggtatgtct tggaaacgga gtccatctgt ttccaaaata gtatttccta
     4141 aaacaaagaa atcccacaaa ataattttga aatacattgt gttctgctct gcaccttgca
     4201 gaggacagtg actattaact cgaaagactt gcagactttg agaaaaaaac aaaacaaaaa
     4261 gaccccacat aactgcaaaa accctacttt gcccaaacta gtttactctt ttaaaacgtt
     4321 tgctgagtct ggttgcttac ctgtaatacg actggggagt atgaggcagg aagatacgtt
     4381 ggggcctaag aactagatag atagcaagac cttgtttcaa attgaaacaa taaaggaact
     4441 gtagcatccc ataagaacat aggagaaacg attgtctctg agcgccacgg tgtggtcgtt
     4501 agtgacagtg agtaacacct cttccccaga gcctgatgct aattcagatc tgctccattg
     4561 accatgcaca tagggtcatc ctgagtctgg gcttgtacca catgctggtt ttcctaatca
     4621 tatgatgctt tttaggtaga acatctattc tttttttccc ttagagggtg actgaaaagt
     4681 cctagacaag gttacttatg gaaaaccaga agagtaagag agcagtggct ttctgttgtg
     4741 gcttcatgga tagcaagcca gttttattca tttttcaatg gccactgaac gttttctaag
     4801 tccttttatt taggaaacca aaattgaagc tatctcaaag taaatctaca gggagagaga
     4861 aaaccaaacc attcacaagt tagggttctg ttttgatcaa gaactcttgt aaaaggccct
     4921 tgcaacatct ggttatctgc agagagaagc taggagccta agctaagcag aaacattaag
     4981 accctctgtg ttagggaatt gggtatatgt agcacagtaa ttctctactt tatgcaagta
     5041 agtattcaag aatatttact taattctcaa tctactcttt tcttagctag tttgtactca
     5101 gggaacacaa atttaataca aatgaaatga aacacaattt ggaaaataat cgtaaacaac
     5161 cgaatcagat ttttatactg cttcatctgt agtctctgct aacatgcaag catgcttagt
     5221 taatgtgggc cccacactga tgttcataag acttaacagc ctggaacata cctaacacag
     5281 ataccacagc tctgcctggt cactcccagg gtgaagtctc atacctaaat gtgagactgc
     5341 ccgtagactt gtctgctttg tgtttggttc agtctggcaa cttgtccttc agttctctgt
     5401 ggcttgttaa gtagggctta tggcttagta gttcttggca actctgttga aaactctgga
     5461 tagtaagtgt gatctaggac ttggcctcaa tttaaaaccc taaaatgaaa gggatggaca
     5521 cagagctatc gtccctctga gctctgaagt catgccttgt ttactacttc agagcgtcag
     5581 tgtgagtgtt cacattttat gtatttgttt gctagtatga gtgttttttc cttagtccga
     5641 ggcatttcaa aggtacacta accaaaagta cttggactac tggaatgcaa aactgaaaac
     5701 atggtgtgga tttagcattc attagaagat gactgctagg tagcagtggt ggggcccatg
     5761 catttccagg tctgctagtg ctccctcgca gtacccagtc cagtaagcag gctcactgca
     5821 gttgatcatc ttctgggctg aacagaaaga gtttgtgaaa catctgagag tcaagtaata
     5881 aaaataaaca ctggtgtgtt gtttgtaagt cctggaatga catatagtag ttataagtga
     5941 atgtagttag tctaattata aaagctagtt gattgcacta actagatgta attttgtgaa
     6001 atatttatga ttatattgat tttattataa gtatttataa gttattgagg gtgcttaaaa
     6061 tccagaaaaa tctctaggac atattttttt ttctgaaatt gtatttatat gatttgggtg
     6121 tagggagttt tatactggtg actgagccca ggcttcccgt gtaagaagca agggcttaac
     6181 ttcaacctca atcccctggc cctctacatg attctgaaag tccaggcgct ctttgtcgag
     6241 gaaaattagc cccagcccga tctaaatgat cacagtttgt gactgctagc tgaactaggc
     6301 ttatctaaat ttcattttgg ccatttttaa ttttgtgcat aaatgagatg acactgtttt
     6361 tatacagtgt gtctctctga gctacctaat cactattggc tctgatagaa gttcaccaag
     6421 cctttttaaa cttggaaacg taacttaagt taatgagctt taagtcatgc atatatgtat
     6481 gtgtatatat acatatggat tatgtaaaca agttagcagc ggcgtcttta tcagcggcgc
     6541 ctttccttga atatttatga tgcggttgat gctactgaac agtaataccg tgaggaacag
     6601 gccccagtct agactctaga ctggatggag ttttgattaa gcttcttcag ccataacctg
     6661 ttcagaatca ggtccaatcc agacgttatc agactgcagg cctgtatttt gtgagccaac
     6721 attaatgttt actgccagcc ttgcatagca gtgccggttg cgactgcatg tgacttttag
     6781 taactgtagt ttatgtctaa aagcagttca agttttatag aggtaattca tgattatttg
     6841 aacaagcagg gtgggtaatt caaatatatt taaagtgcaa ttaaggaaat tctagggtat
     6901 aatattcttt ccagttttaa aaaaaatagt taaggatttt tagaatgtcc tagaatgaaa
     6961 aaaatttttt ttggacagac tttttaaaaa acaaggttta tacagatttt tttttttaat
     7021 tcaaggtttg ctactttgtg tttatttaaa tttgcaaaga gaaaccgaag cctcctagga
     7081 aagagttgca ttgttatttt tctcctgcta gtctgaatgt gcattttcac agctaaaagt
     7141 cacttgtaaa taaggtttgt taccacattc gaagcccatg ttttctaaat ggagaacatt
     7201 ttcccagaat caaagagaca tagacccact gtctacacaa agaaagaaag cgtacctggt
     7261 gccttctcaa ataattgtag agtcaaagtg aacgtgttcc aagtctgccc caccccaccc
     7321 cctctcctgt tcctcgggtt ctctgtgacc tacctgtccc tcccacagac ggtcacagga
     7381 tttgcttctg ccctttcagt tctacttgac caaacctcac agcatcacca gtgacgttct
     7441 tcactgaata cattttctct ttcacttatt tcacttttta aagccacctg ggaaaatatt
     7501 tggctgcctt cttatgcttt taaaatgaat gcaattatta gcagtgttta tatttgaaag
     7561 tttataaagg aaagaaattc agagctaagg actgactctt aattggatct aattccctgt
     7621 gtgttacgat agcagcacaa gttatgtaac cttgtgtagt tttctcttgt acaaaatgtg
     7681 gatgataacc tacctcacag atgtgtgtga gaagccgttt caaagcccct aagtaggggt
     7741 ttatagtccc atcctcagtg tcaatcctcc cttttttact agacaccttt gagaatggta
     7801 ggatgagtat cttgttttta gatgtcatat gtaataaggg catagtgctg agaatgttta
     7861 agacacagtt tcacaaatca tagtgttctt actgtaagac acttaaaaat ttcttgaaaa
     7921 cctcaaatgt ttaaatatcg aatctgtctt ttgtttcaat aaatattgtt attcaaagtg
     7981 ttgtctgaag ctgccttaat gtaccaaaat acaactgtca aaactgttca caactatatt
     8041 catggttgta ctgttttgag tgttcatttt gaagttgcta tgaactatga ccttgaagga
     8101 tagtattttg atattatttt ggtaggaata gttgtggttt tttttttcat catgtttaac
     8161 agtgactttt gtgtgtaaaa aataaaactg aaacttggca g
//
