LOCUS       NM_001001184            3835 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus primase and polymerase (DNA-directed) (Primpol),
            transcript variant 1, mRNA.
ACCESSION   NM_001001184
VERSION     NM_001001184.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Torregrosa-Munumer,R., Forslund,J.M.E., Goffart,S., Pfeiffer,A.,
            Stojkovic,G., Carvalho,G., Al-Furoukh,N., Blanco,L., Wanrooij,S.
            and Pohjoismaki,J.L.O.
  TITLE     PrimPol is required for replication reinitiation after mtDNA damage
  JOURNAL   Proc Natl Acad Sci U S A 114 (43), 11398-11403 (2017)
   PUBMED   29073063
  REMARK    GeneRIF: PrimPol can reinitiate stalled mtDNA replication and can
            prime mtDNA replication from nonconventional origins.
REFERENCE   3  (bases 1 to 3835)
  AUTHORS   Pilzecker,B., Buoninfante,O.A., Pritchard,C., Blomberg,O.S.,
            Huijbers,I.J., van den Berk,P.C. and Jacobs,H.
  TITLE     PrimPol prevents APOBEC/AID family mediated DNA mutagenesis
  JOURNAL   Nucleic Acids Res 44 (10), 4734-4744 (2016)
   PUBMED   26926109
REFERENCE   4  (bases 1 to 3835)
  AUTHORS   Probert,F., Rice,P., Scudamore,C.L., Wells,S., Williams,R.,
            Hough,T.A. and Cox,I.J.
  TITLE     (1)H NMR metabolic profiling of plasma reveals additional
            phenotypes in knockout mouse models
  JOURNAL   J Proteome Res 14 (5), 2036-2045 (2015)
   PUBMED   25849460
REFERENCE   5  (bases 1 to 3835)
  AUTHORS   Bianchi,J., Rudd,S.G., Jozwiakowski,S.K., Bailey,L.J., Soura,V.,
            Taylor,E., Stevanovic,I., Green,A.J., Stracker,T.H., Lindsay,H.D.
            and Doherty,A.J.
  TITLE     PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA
            replication
  JOURNAL   Mol Cell 52 (4), 566-573 (2013)
   PUBMED   24267451
REFERENCE   6  (bases 1 to 3835)
  AUTHORS   Garcia-Gomez,S., Reyes,A., Martinez-Jimenez,M.I., Chocron,E.S.,
            Mouron,S., Terrados,G., Powell,C., Salido,E., Mendez,J., Holt,I.J.
            and Blanco,L.
  TITLE     PrimPol, an archaic primase/polymerase operating in human cells
  JOURNAL   Mol Cell 52 (4), 541-553 (2013)
   PUBMED   24207056
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK030772.1, BC065112.1 and AC119267.8.
            
            On Oct 26, 2017 this sequence version replaced NM_001001184.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065112.1, SRR7345562.5013298.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate          :: translation inferred from conservation
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AK030772.1         2-28
            28-3824             BC065112.1         1-3797
            3825-3835           AC119267.8         146536-146546
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 26.39 cM"
     gene            1..3835
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /note="primase and polymerase (DNA-directed)"
                     /db_xref="GeneID:408022"
                     /db_xref="MGI:MGI:3603756"
     exon            1..94
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            95..171
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            172..357
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..1791
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /EC_number="2.7.7.7"
                     /EC_number="2.7.7.102"
                     /note="coiled-coil domain-containing protein 111"
                     /codon_start=1
                     /product="DNA-directed primase/polymerase protein"
                     /protein_id="NP_001001184.1"
                     /db_xref="CCDS:CCDS40334.1"
                     /db_xref="GeneID:408022"
                     /db_xref="MGI:MGI:3603756"
                     /translation="MLRKWEARVKQIEERASHYERKPLSSVYRPRLAKPEEPSSIWKL
                     FHRQNQAFNFVKSCKESVHVFALECKRGNGQRIYLVTSYAQLWFYYKTRKTLLHCYEV
                     IPENAVCKLYFDLEFNKLANPGADGKMMVALLIQHVCKALEEFYNVQCSAEDVFNLDS
                     STEEKFSRHLIFQLHNVAFKDNRHAGNFVRKILQPALHLIAEDDEAKVPEAVGQDASG
                     FSVTPLKQEISEAREKVGLPKQCDPDLSFLVVKNHMGEKCLFVDLGVYTKNRNFRLYQ
                     SSKIGKCVSLEVAEDNRFIPKQSKDISEENQYFLSSLVSNVRFSDTLRVLTCHPSQTK
                     RKRAECFNSTGTSVESIEGFQGSPYPEVDQFVLSLVNKHDIKGGIRRWNYFFPEELLV
                     YDICKYRWCENIGRAHKSNNIMILVDLKNEVWYQKCHDPVCKAQNFKSTCSPLPTEVS
                     LLFLLKDEDFTSGETDDTSTSLTKDSQTPPSCNLSAGGLSAAAWDDEDDALFLEATED
                     AEFADAADKSLGSMDDIPDELIIEALQNS"
     exon            358..455
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            456..585
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            586..733
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            734..967
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1130
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1219
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1309
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1418
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1501
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1548
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..2696
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2095..2100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /note="hexamer: ATTAAA"
     polyA_site      2118
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /note="major polyA site"
     exon            2697..3835
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3238..3243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
                     /note="hexamer: AATACA"
     polyA_site      3257
                     /gene="Primpol"
                     /gene_synonym="Ccdc111"
ORIGIN      
        1 tccggctccg ccccctcgcg cgggtttcaa gctctggttc ccgccatctc tggctccgcc
       61 agctgccgcc tccggccgac ttgcgccact tgagggaata tgttatttgc taatgagatt
      121 acgtcggttc aaagaattct gctaaaattg aagacaaagg cattaatata ggaagcaatg
      181 ttgaggaaat gggaggcaag ggtgaagcag attgaagagc gggcatccca ctatgagagg
      241 aagccattgt cttccgtgta cagaccaaga ctggccaagc cagaagaacc atcctccatt
      301 tggaagctat ttcaccgtca gaatcaagct tttaattttg ttaaaagctg taaagagagc
      361 gttcacgtat ttgctttgga gtgcaaaagg ggaaatggac aacgtattta ccttgtaacg
      421 tcctatgccc aactttggtt ttactataag acccggaaaa ccctcttgca ctgctatgaa
      481 gttattcctg agaatgctgt gtgcaagctt tattttgatt tagaatttaa caaattggcc
      541 aacccaggag ctgatgggaa gatgatggtt gcattgctca tacagcatgt ttgtaaagca
      601 cttgaggagt tttacaatgt tcagtgctca gccgaagatg ttttcaacct ggattccagc
      661 actgaagaga aatttagccg acacctaata tttcagctcc acaatgtggc atttaaagat
      721 aacaggcatg ctggtaattt tgtgagaaaa attttgcaac ctgctcttca cttgattgcc
      781 gaggatgatg aagctaaggt cccagaggcg gtgggccagg acgcctccgg tttttctgtt
      841 acaccattaa aacaagagat ttcagaggcc cgggagaagg tggggttgcc taagcagtgt
      901 gaccccgatc tttcattttt agttgtgaaa aatcacatgg gagaaaaatg cctttttgtg
      961 gatctaggag tgtatacaaa aaacagaaac tttcgactgt atcagtcatc caaaatagga
     1021 aagtgtgtgt ctttggaggt tgctgaagat aacagattta ttccaaaaca gtcaaaagat
     1081 atttctgagg aaaaccagta tttcctatct tccctggtga gcaatgtcag attctcagat
     1141 actctgcgag ttctcacatg ccacccatct cagactaaac gaaagcgggc tgagtgtttt
     1201 aacagtactg gcacttcagt ggagtccatt gaaggcttcc agggctcccc ataccctgag
     1261 gttgaccaat ttgttctttc tttggtgaac aaacatgaca ttaaaggagg gattcggcgt
     1321 tggaactact ttttcccgga agaattactg gtttatgata tatgcaaata ccgctggtgt
     1381 gaaaacattg gaagagccca caagagtaat aatataatga ttctggttga tctgaagaat
     1441 gaagtttggt atcagaaatg ccatgaccct gtctgtaaag cacaaaactt caaatctacc
     1501 tgttccccat tacccactga agtaagtctg ctatttcttc tgaaagatga agacttcact
     1561 tctggagaaa cagatgacac cagcacctca ctgaccaagg attctcaaac cccgccatcc
     1621 tgcaacttgt cagccggcgg gctttctgcc gctgcctggg atgacgaaga tgacgccctt
     1681 ttcttagaag cgacagaaga tgctgaattt gctgacgctg cagataagag ccttggttca
     1741 atggatgata ttcctgatga actaattata gaagctttac agaatagctg actgtggaca
     1801 gaacatacat aaccaggcta caatttgcct gaagcctgag atttgataac ataaatgtta
     1861 aattagtgcc gggactgtag cccaacatta gaatacttac ctaagatgtg caaggccctg
     1921 catttaacat catacaaaag atcaacttat taacttattg taaaacctgt ttatagcaac
     1981 cgttctatca cttggtttac ctaagccaga tttattgaag actacctttg acatatttca
     2041 gcagacgctc ctaattaacc aggatcattc tatgcttgca tttgcactga catcattaaa
     2101 gtttattgta gttaaaaagc ttatgcctgg tgatgactaa cctgtcaaga gactgaaatt
     2161 tattaacaca atactcaaaa atatggctat gccttctgag ttattagaca actgaattgg
     2221 tgtaatttag tttgaatgac aaaacggtag tacaccctga taagcaggtc tcactactgt
     2281 caccacaggc cgctgctgtc aggagcatgg cttcaggagc agaaaaggca gcaggaggct
     2341 cagttctcag tccagctcga gaagcttccc cagctgatag ttgatggttc tcaggtggtt
     2401 ttcggcgccc agaatgcttc ttgctttaaa taacagagct ggaaggctag atgaatcata
     2461 ctgttgataa aaggaaaaca atttagactc acaaatagcg atgctttgct atctttttag
     2521 ttcctaaccc taaccaccaa ggccgatctt gatagttgtc caccagctct cttttgttgc
     2581 tctgggatca ttaggaaaca ggttttggcc catctcctct cctctcagaa cataactgct
     2641 aataccacac catgaggaac tgctcgacaa agctctcaca gatctctgta ccccagcccc
     2701 tcaggaccct cctgccattt caatgaaaca gcttctaaag tatgaacatg ggctggacaa
     2761 aggtggttgg cctttacagc acgtctgagt gggctgagga ctgatggaaa ctgataaagc
     2821 agagcgcagt tgttcaactc agacttgtgc acagtacaca ccgcctagaa gatctgagga
     2881 gtaaccgagt cttccttccc ttcagcagga agctagaagg tgatgctgtt gtctcactga
     2941 cttacaataa tgctgtccgt tacctttctg caaactgctg ccactctaca agcaaatgcc
     3001 tggatccctt ctgatgtgca gtaaccagga ctcacctttc ccgttccctt tggtcctttc
     3061 tcttgaacgt ttgaataatc ttgacacagc tgttttaaat tagataagaa atgcttggac
     3121 ttcttaagtg atgactttct ctccttaagg tcatcatatt ttgtttgtag ttgtttcagt
     3181 tgtggttcta atctagataa caaaattttt ttttaatctc aaaaacaaat tttacaaaat
     3241 acaaattaat agaaatatat gtggtgtttg tggactgaaa atattaaggt gcccacatta
     3301 taaaaaacaa aaacgcaaac caaaaataac aaaacatttc tcccatataa acaacataga
     3361 gtgtacaagc atgaacatcc tgggtgaggg aagaacagaa cagaagtgga cttggtggcc
     3421 tggctcgcct cgcacactat gaacgtgtaa atgatcctag gtttctgctc aacgcaactg
     3481 tgacacacag tgagcagtga cagccatggg gacatgcctc acagatgaca aaatccacca
     3541 aattactcaa gtcctgggaa ctactgcagt caatataaat ttggctgaca ttttctgagc
     3601 ataggcagga gtagccagcc tgacagctta accaggaggt catgcagaaa gttccccttt
     3661 taaaaatcct tagtttctgt ggccaaataa aatcagcaga aaaactgtcc cagagcctgg
     3721 agagaaagat gtgtgacctg ggcgaactgc ctgtgtctga ggcttagttt cttcacctat
     3781 aaaagaaaag ggaatttgca aataaaaata taaaaaaaac ctaagcttga attga
//
