LOCUS       NM_008921               1533 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus DNA primase, p49 subunit (Prim1), mRNA.
ACCESSION   NM_008921
VERSION     NM_008921.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1533)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   4  (bases 1 to 1533)
  AUTHORS   Mizuno,T., Yamagishi,K., Miyazawa,H. and Hanaoka,F.
  TITLE     Molecular architecture of the mouse DNA polymerase alpha-primase
            complex
  JOURNAL   Mol Cell Biol 19 (11), 7886-7896 (1999)
   PUBMED   10523676
REFERENCE   5  (bases 1 to 1533)
  AUTHORS   Mizuno,T., Okamoto,T., Yokoi,M., Izumi,M., Kobayashi,A.,
            Hachiya,T., Tamai,K., Inoue,T. and Hanaoka,F.
  TITLE     Identification of the nuclear localization signal of mouse DNA
            primase: nuclear transport of p46 subunit is facilitated by
            interaction with p54 subunit
  JOURNAL   J Cell Sci 109 (Pt 11), 2627-2636 (1996)
   PUBMED   8937981
REFERENCE   6  (bases 1 to 1533)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur J Biochem 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   7  (bases 1 to 1533)
  AUTHORS   Warden,C.H., Mehrabian,M., He,K.Y., Yoon,M.Y., Diep,A., Xia,Y.R.,
            Wen,P.Z., Svenson,K.L., Sparkes,R.S. and Lusis,A.J.
  TITLE     Linkage mapping of 40 randomly isolated liver cDNA clones in the
            mouse
  JOURNAL   Genomics 18 (2), 295-307 (1993)
   PUBMED   8288233
REFERENCE   8  (bases 1 to 1533)
  AUTHORS   Miyazawa,H., Izumi,M., Tada,S., Takada,R., Masutani,M., Ui,M. and
            Hanaoka,F.
  TITLE     Molecular cloning of the cDNAs for the four subunits of mouse DNA
            polymerase alpha-primase complex and their gene expression during
            cell proliferation and the cell cycle
  JOURNAL   J Biol Chem 268 (11), 8111-8122 (1993)
   PUBMED   8463324
REFERENCE   9  (bases 1 to 1533)
  AUTHORS   Adler,D.A., Tseng,B.Y., Wang,T.S. and Disteche,C.M.
  TITLE     Physical mapping of the genes for three components of the mouse DNA
            replication complex: polymerase alpha to the X chromosome, primase
            p49 subunit to chromosome 10, and primase p58 subunit to chromosome
            1
  JOURNAL   Genomics 9 (4), 642-646 (1991)
   PUBMED   2037291
REFERENCE   10 (bases 1 to 1533)
  AUTHORS   Prussak,C.E., Almazan,M.T. and Tseng,B.Y.
  TITLE     Mouse primase p49 subunit molecular cloning indicates conserved and
            divergent regions
  JOURNAL   J Biol Chem 264 (9), 4957-4963 (1989)
   PUBMED   2925677
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC131120.4.
            
            On Nov 17, 2006 this sequence version replaced NM_008921.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D13544.1, AK089063.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               AC131120.4         135236-135372
            138-295             AC131120.4         135985-136142
            296-402             AC131120.4         137997-138103
            403-476             AC131120.4         138169-138242
            477-613             AC131120.4         140151-140287
            614-672             AC131120.4         141694-141752
            673-782             AC131120.4         142884-142993
            783-874             AC131120.4         143604-143695
            875-1013            AC131120.4         143802-143940
            1014-1083           AC131120.4         146539-146608
            1084-1169           AC131120.4         147074-147159
            1170-1268           AC131120.4         149207-149305
            1269-1533           AC131120.4         149787-150051
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 76.39 cM"
     gene            1..1533
                     /gene="Prim1"
                     /note="DNA primase, p49 subunit"
                     /db_xref="GeneID:19075"
                     /db_xref="MGI:MGI:97757"
     exon            1..137
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1288
                     /gene="Prim1"
                     /EC_number="2.7.7.102"
                     /note="p49; DNA primase 49 kDa subunit"
                     /codon_start=1
                     /product="DNA primase small subunit"
                     /protein_id="NP_032947.1"
                     /db_xref="CCDS:CCDS36086.1"
                     /db_xref="GeneID:19075"
                     /db_xref="MGI:MGI:97757"
                     /translation="MEPFDPAELPELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHRE
                     FSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQ
                     EKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYS
                     GRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEKVHPFVRKSINI
                     IKKYFEEYALVGQDILENKENWDKILALVPETIHDELQRGFQKFHSSPQRWEHLRKVA
                     NSSQNMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPID
                     FHKVDQFDPFTVPTISAICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVF
                     EQFLENLDKSRKGELLKKSDLQKDF"
     exon            138..295
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            296..402
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..476
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            477..613
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..672
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            673..782
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            783..874
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1013
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1083
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1169
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1268
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1533
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1470..1475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prim1"
                     /note="hexamer: AATAAA"
     polyA_site      1495
                     /gene="Prim1"
                     /note="major polyA site"
ORIGIN      
        1 gtgtcagtcc tgtccgggtc tagttcttta cttcatggag ccatttgatc ctgcggagct
       61 gccggagcta ctcaagcttt attaccgaag gctctttccc tacgcccagt actatcgctg
      121 gctcaactat ggcggagtga caaagaatta ctttcaacac cgtgaatttt cattcacact
      181 gaaagatgat atttacattc gctaccagtc cttcaacaac cagagtgaac tggagaagga
      241 gatgcagaaa atgaatccat ataagattga tataggcgca gtatattctc acagacccaa
      301 tcaacacaat acagtgaagc tgggagcttt ccaggcccag gagaaagaac tggtctttga
      361 catcgacatg acagactacg atgacgtaag gagatgctgc agttctgcag acatatgttc
      421 caaatgctgg actctcatga caatggccat gcgcatcata gaccgggcat tgaaggagga
      481 ctttggattt aagcaccgcc tctgggtcta ttcaggaagg agaggtgtgc actgttgggt
      541 ctgtgatgaa tccgttcgaa agctgtcctc tgctgtgcgc tccgggatcg tggagtactt
      601 gagtcttgta aagggtggtc aagatgttaa aaagaaagtt cacctaaatg agaaagttca
      661 cccttttgtc agaaaatcta taaacataat taaaaagtac tttgaggaat atgccttagt
      721 cggccaagat attcttgaaa ataaagaaaa ctgggataag attttagccc ttgttcctga
      781 gacaattcat gatgagctgc aaagagggtt ccagaagttc cacagttcac ctcagcgctg
      841 ggaacacctg aggaaagtgg ccaactcatc tcagaatatg aagaacgata agtgcggccc
      901 ctggctggag tgggaggtca tgctccagta ctgtttccca cgcctggatg tcaacgtcag
      961 caagggagtc aatcacttac tgaagagccc tttcagtgtt catcccaaga caggtcggat
     1021 ttctgtgcct atcgattttc ataaagtgga tcaatttgat ccgtttactg tcccaactat
     1081 aagtgccatc tgccgggaat tggatatggt ttccactcat gagaaggaaa aggaggagaa
     1141 tgaagctgac agcaaacaca gagtcagagg ttacaagaag accagtctag caccgtatgt
     1201 gaaagtattt gaacagtttc ttgaaaacct ggataaatcc cggaaagggg aacttctcaa
     1261 gaagagcgat ttacaaaaag atttctgaag aaaacagctc ccatcaaacc aacgtgaatg
     1321 ccttctacct tcatccatgg agcatttaat aaatacagta gaatcatcaa tgggcctcaa
     1381 tccttgaagc agaactttct ttcgagaagt aatttatgtc aaggagacct ggtttgtgag
     1441 accaaagagg gtgtcaagag ccctgaaaca ataaatcaag aataagtgaa ctgcagaagt
     1501 ttgtaaaatg tattatataa aaagataatc ctt
//
