LOCUS       NM_021400               4278 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus proteoglycan 4 (megakaryocyte stimulating factor,
            articular superficial zone protein) (Prg4), transcript variant 1,
            mRNA.
ACCESSION   NM_021400
VERSION     NM_021400.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Song,C., Sawall,J.K., Ji,X., Song,F., Liao,X., Peng,R., Ren,H.,
            Koyama,E., Pacifici,M. and Long,F.
  TITLE     Tgfbeta signaling stimulates glycolysis to promote the genesis of
            synovial joint interzone in developing mouse embryonic limbs
  JOURNAL   Sci Adv 11 (2), eadq4991 (2025)
   PUBMED   39772668
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   3  (bases 1 to 4278)
  AUTHORS   Tanguay,A.P., Menon,N.G., Boudreau,M.H., Jastrzebski,S.,
            Woods,P.S., Doyle,E.A., Edwards,W.B., Jay,G.D., Deymier,A.C.,
            Lorenzo,J., Lee,S.K. and Schmidt,T.A.
  TITLE     PRG4 deficiency in mice alters skeletal structure, mechanics, and
            calvarial osteoclastogenesis, and rhPRG4 inhibits in vitro
            osteoclastogenesis
  JOURNAL   J Orthop Res 42 (6), 1231-1243 (2024)
   PUBMED   38111181
  REMARK    GeneRIF: PRG4 deficiency in mice alters skeletal structure,
            mechanics, and calvarial osteoclastogenesis, and rhPRG4 inhibits in
            vitro osteoclastogenesis.
REFERENCE   4  (bases 1 to 4278)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 4278)
  AUTHORS   Fidler,T.P., Dunbar,A., Kim,E., Hardaway,B., Pauli,J., Xue,C.,
            Abramowicz,S., Xiao,T., O'Connor,K., Sachs,N., Wang,N.,
            Maegdefessel,L., Levine,R., Reilly,M. and Tall,A.R.
  TITLE     Suppression of IL-1beta promotes beneficial accumulation of
            fibroblast-like cells in atherosclerotic plaques in clonal
            hematopoiesis
  JOURNAL   Nat Cardiovasc Res 3 (1), 60-75 (2024)
   PUBMED   38362011
REFERENCE   6  (bases 1 to 4278)
  AUTHORS   Panagopoulos,I., Mertens,F., Isaksson,M. and Mandahl,N.
  TITLE     Expression of DOL54 is not restricted to myxoid liposarcomas with
            the FUS-DDIT3 chimera but is found in various sarcomas
  JOURNAL   Oncol Rep 12 (1), 107-110 (2004)
   PUBMED   15201968
  REMARK    GeneRIF: Expression is characteristic ofmyxoid liposarcoma and is
            also a frequent finding also in various other sarcomas.
REFERENCE   7  (bases 1 to 4278)
  AUTHORS   Kuznetsov,N.V., Sandblad,L., Hase,M.E., Hunziker,A., Hergt,M. and
            Cordes,V.C.
  TITLE     The evolutionarily conserved single-copy gene for murine Tpr
            encodes one prevalent isoform in somatic cells and lacks paralogs
            in higher eukaryotes
  JOURNAL   Chromosoma 111 (4), 236-255 (2002)
   PUBMED   12424524
REFERENCE   8  (bases 1 to 4278)
  AUTHORS   Koshizuka,Y., Ikegawa,S., Sano,M., Nakamura,K. and Nakamura,Y.
  TITLE     Isolation of novel mouse genes associated with ectopic ossification
            by differential display method using ttw, a mouse model for ectopic
            ossification
  JOURNAL   Cytogenet Cell Genet 94 (3-4), 163-168 (2001)
   PUBMED   11856874
REFERENCE   9  (bases 1 to 4278)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   10 (bases 1 to 4278)
  AUTHORS   Kuroda,M., Wang,X., Sok,J., Yin,Y., Chung,P., Giannotti,J.W.,
            Jacobs,K.A., Fitz,L.J., Murtha-Riel,P., Turner,K.J. and Ron,D.
  TITLE     Induction of a secreted protein by the myxoid liposarcoma oncogene
  JOURNAL   Proc Natl Acad Sci U S A 96 (9), 5025-5030 (1999)
   PUBMED   10220412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161432.5.
            
            On Nov 27, 2007 this sequence version replaced NM_021400.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data because no single transcript was available for the full length
            of the gene. The extent of this transcript is supported by
            orthologous human data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN04095720
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC161432.5         67381-67397
            18-116              AC161432.5         67950-68048
            117-239             AC161432.5         71327-71449
            240-359             AC161432.5         72775-72894
            360-503             AC161432.5         75254-75397
            504-623             AC161432.5         75939-76058
            624-2909            AC161432.5         77209-79494
            2910-2987           AC161432.5         80006-80083
            2988-3124           AC161432.5         81187-81323
            3125-3281           AC161432.5         81536-81692
            3282-3482           AC161432.5         82097-82297
            3483-3608           AC161432.5         82658-82783
            3609-4278           AC161432.5         83465-84134
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 63.84 cM"
     gene            1..4278
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="proteoglycan 4 (megakaryocyte stimulating factor,
                     articular superficial zone protein)"
                     /db_xref="GeneID:96875"
                     /db_xref="MGI:MGI:1891344"
     exon            1..17
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            18..116
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..3706
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; proteoglycan 3; superficial zone proteoglycan;
                     megakaryocyte-stimulating factor"
                     /codon_start=1
                     /product="proteoglycan 4 isoform 1 precursor"
                     /protein_id="NP_067375.3"
                     /db_xref="CCDS:CCDS48392.1"
                     /db_xref="GeneID:96875"
                     /db_xref="MGI:MGI:1891344"
                     /translation="MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATC
                     NCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDS
                     FCEEVHNPTSPSPKTAPPPPGASHTIKSTTKRSPKSPKKKTKKVVESEEITEEHSVSE
                     NQESSSSSSSSSSTIRKIKSSKNSANRELQKNPKVKDNKKNTPKKKPNPEPPAVDEAG
                     SGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASN
                     KETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKK
                     PVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPT
                     TPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRK
                     EPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEP
                     EPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEP
                     TTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTT
                     LKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPK
                     EPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEP
                     EPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLA
                     PKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKK
                     PKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDA
                     DGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTT
                     LRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDS
                     QYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYT
                     YKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQD
                     KGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARA
                     ITTRSGQTLSKIWYNCP"
     exon            117..239
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            240..359
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            360..503
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            504..623
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            624..2909
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..2987
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3124
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3125..3281
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3482
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3608
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..4278
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3784..3789
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      3825
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="major polyA site"
     regulatory      3851..3856
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="hexamer: ATTAAA"
     polyA_site      3874
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
     regulatory      4012..4017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="hexamer: TATAAA"
     polyA_site      4030
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
     regulatory      4116..4121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /note="hexamer: ATTAAA"
     polyA_site      4142
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
ORIGIN      
        1 atctctcctt tttacagcaa gggcgttgca tccgagaacc atggggtgga aaatacttcc
       61 cgtctgcttg tcgctgctgc ttcctgttgt cttgattcaa caggtttcat ctcaagattt
      121 atcaagctgt gcagggagat gtggggaagg gtactctaga gatgccacct gcaactgtga
      181 ttataactgt caacactaca tggagtgctg tcctgatttc aagagagtct gcactacaga
      241 gctctcctgc aaaggacgct gcttcgagtc cttcgctcga gggagggagt gtgactgtga
      301 ctcccagtgt aaacagtatg gcaagtgctg tgcagattat gacagcttct gtgaagaagt
      361 gcataatccc acatcaccat ctccaaagac tgcacctccg cctccaggag catctcacac
      421 catcaaatca actaccaaac gctcacccaa atcaccaaaa aagaagacta agaaagttgt
      481 agaatctgag gaaataacag aagaacattc tgtttctgaa aatcaagagt cctcctcctc
      541 ctcctcttct tcctcttcaa ctattcggaa aatcaagtct tccaaaaatt cagctaatag
      601 agaattacag aagaacccaa aagtaaaaga taacaagaaa aacactccta aaaagaaacc
      661 caatccagaa ccgccggctg tggatgaagc tggaagcggg ctggataatg gtgagttcaa
      721 gctcacccca cctcctcctg accctcctac tactccacac agcaaagtca ccacatctcc
      781 caagactaca gcagcaaaac cagtaactcc taaacccagt cttgcaccta attctgagac
      841 atccaaagag gcatctttag catccaataa agagacaaca gttgaaacga aagagactac
      901 tgcaacaaat aaacaatctt ctgccagtaa aaaaaagacg acttcagcta aagagacacg
      961 gagtgcagag aaaacatctg ataaagatgt tgagcccaca tctacaactc ccaagaactc
     1021 agctcccact actaccaaga agcctgttac tacaaccaag gaaccagttc ctaccactcc
     1081 caaggagcct gaacccacaa ctcccaagga accacctcca accaccaaga agcctgaacc
     1141 caccaccccc aaggagcctg aacccacaac tcccaaggag cctgaaccca ccacccccaa
     1201 ggagcctgaa cccaccactc ccaaggagcc tgaacccaca actcccaagg aaccacctcc
     1261 aaccaccaag aagcctgaac ccaccacccc caaggagcct gaacccacaa ctcccaagga
     1321 gcctgaaccc accaccacca aggagcctga acccaccacc accaaggagc ctgaatccac
     1381 cacccgcaag gagcctgaac ccaccactcc caaggagcct gaacccacaa ctcccaagga
     1441 gcctgaacct acaaccccca aggagcctga acccacaact cccaaggagc ctgaacccac
     1501 cactctcaag gagcctgaac ccaccacgcc caaggagcct gaacccacaa ctcccaagga
     1561 gcctgaacct acaaccccca aggagcctga acccacaact cccaaggagc ctgaacccac
     1621 aactcccaag gagcctgaac ccacaactcc caaggagcct gaacccacaa ctcccaagga
     1681 gcctgaaccc accacgccca aggagcctga acccacaacc cccaaggagc ctgaacctac
     1741 aacccccaag gagcctgaac ccacaactcc caaggagcct gaacccacca ctctcaagga
     1801 gcctgaacct acaaccccca aggagcctga acccaccact cccaaggagc ctgaacccac
     1861 cactctcaag gagcctgaac ccaccacgcc caaggagcct gaacccacca ctctcaagga
     1921 gcctgaacct acaaccccca aggagcctga acccaccacc accaaggagc ctgaatccac
     1981 cacccgcaag gagcctgaac ccaccactcc caaggagcct gaacccacaa ctcccaagga
     2041 gcctgaaccc acaactccca aggagcctga acccaccacg cccaaggagc ctgaacccac
     2101 aactcccaag gagcctgaac ccaccacgcc caaggagcct gaacccacaa cccccaagga
     2161 gcctgaaccc acaactccca agaaacctga acccacaact cccaaggagc ctgaacctac
     2221 aacccccaag gagcctgaac ccaccacccc caaggagcct gaacccacca cgcccaagga
     2281 gcctgaaccc acaactcgca aggagcctga acctacaact cccaaggagc ctgaacctac
     2341 aacccccaag gagcctgaac ccacaactcc caagaagcct gaacccacca ccacatcacc
     2401 caaaaccact actctgaaag caacaactct tgcacccaaa gtaactgctc cagcagaaga
     2461 gattcagaac aaacctgaag aaacaactcc tgcatcagaa gattctgatg attctaaaac
     2521 aactctaaaa ccacagaagc caaccaaagc acccaagcct accaaaaagc caaccaaagc
     2581 acccaagaag cccacctcta ccaaaaagcc aaagacacca aaaacaagaa aaccaaaaac
     2641 tacaccatct cctctaaaga cgacttcagc aacacctgaa ctgaatacca cccctctaga
     2701 agtcatgctg ccaaccacca ccatccctaa acaaactcca aaccctgaaa cagctgaagt
     2761 aaatccagat catgaagatg cagatggagg tgaaggagaa aaacctctga ttcccgggcc
     2821 ccctgtgcta ttccccacag ctattccagg cactgatctt ttggccggga gactcaatca
     2881 aggcattaac atcaatccca tgctttcaga tgagaccaat ttatgcaatg gtaagccagt
     2941 ggatggactg actacgctgc gcaatgggac attagttgca tttcgaggtc attatttctg
     3001 gatgctgaat ccattcagac caccatctcc accacgcaga attaccgaag tttggggtat
     3061 tccctctcct attgacacag tttttactag atgtaactgt gaaggaaaaa ctttcttctt
     3121 taaggattct cagtactggc gcttcaccaa tgatgtggta gatcctgggt atcctaaaca
     3181 aattgtcaaa ggctttggag gactaacagg gaagatagtg gctgctcttt caatagccaa
     3241 gtacaaggac aggcctgaat ctgtgtactt cttcaagaga ggtggcaaca ttcagcagta
     3301 cacttataaa caggagccaa tgaagaagtg cacagggagg cggcctgcca tcaactactc
     3361 ggtgtacggg gaggcagcac aggtcaggag gcgccgcttt gagcgtgctg ttggaccttt
     3421 ccagacacac accttcagga tccattactc ggtgcccatg agagtttctt atcaagacaa
     3481 aggtttcctc cacaatgaag tcaaagtgag tacaatgtgg agagggtttc caaatgttgt
     3541 tacttcagcg ataacactgc ctaacattag gaaacctgat ggctatgatt actacgcctt
     3601 ttccaaagat caatactata acattgatgt gcctacaaga acagcaagag caattaccac
     3661 acgttcaggg cagaccctct ccaaaatctg gtacaactgt ccttgaactg attgacaaag
     3721 aagagtcatc aaaatgaaat gaagaaaaca gtgatacttt ttgacattga aatacatttt
     3781 attaataaag aatgttgaaa tgagcatacc tatttaaata caaaacagtt ctcaacttga
     3841 caatcattat attaaaacag attcgtcaat cttattcaca tgtgtcacag tttagagatc
     3901 acttaattaa tattccctct atttattcct cctcttcctc ccattgcatg gcttatacct
     3961 gtagaaggaa acggcaaaat tcattttcag tagaaatatc taagatatgg atataaaaag
     4021 atattctcca tgttgactgg taattattat agctgggtta ctagatttat gatgttaata
     4081 cagtctaaat gagttatttt gaagaatgtg aaaatattaa aatcttctat ttctttacta
     4141 gctactaata gccacaaaac tttcggtgca gaaaacaaaa caaaacaaaa cttaacacca
     4201 taagaataca gataatgcac aacatgctaa cacttgattt tccaaatctt tttttggatc
     4261 taatgaaata aaatacac
//
