LOCUS       NP_067375               1221 aa            linear   ROD 04-FEB-2025
DEFINITION  proteoglycan 4 isoform 1 precursor [Mus musculus].
ACCESSION   NP_067375
VERSION     NP_067375.3
DBSOURCE    REFSEQ: accession NM_021400.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1221)
  AUTHORS   Song,C., Sawall,J.K., Ji,X., Song,F., Liao,X., Peng,R., Ren,H.,
            Koyama,E., Pacifici,M. and Long,F.
  TITLE     Tgfbeta signaling stimulates glycolysis to promote the genesis of
            synovial joint interzone in developing mouse embryonic limbs
  JOURNAL   Sci Adv 11 (2), eadq4991 (2025)
   PUBMED   39772668
REFERENCE   2  (residues 1 to 1221)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   3  (residues 1 to 1221)
  AUTHORS   Tanguay,A.P., Menon,N.G., Boudreau,M.H., Jastrzebski,S.,
            Woods,P.S., Doyle,E.A., Edwards,W.B., Jay,G.D., Deymier,A.C.,
            Lorenzo,J., Lee,S.K. and Schmidt,T.A.
  TITLE     PRG4 deficiency in mice alters skeletal structure, mechanics, and
            calvarial osteoclastogenesis, and rhPRG4 inhibits in vitro
            osteoclastogenesis
  JOURNAL   J Orthop Res 42 (6), 1231-1243 (2024)
   PUBMED   38111181
  REMARK    GeneRIF: PRG4 deficiency in mice alters skeletal structure,
            mechanics, and calvarial osteoclastogenesis, and rhPRG4 inhibits in
            vitro osteoclastogenesis.
REFERENCE   4  (residues 1 to 1221)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (residues 1 to 1221)
  AUTHORS   Fidler,T.P., Dunbar,A., Kim,E., Hardaway,B., Pauli,J., Xue,C.,
            Abramowicz,S., Xiao,T., O'Connor,K., Sachs,N., Wang,N.,
            Maegdefessel,L., Levine,R., Reilly,M. and Tall,A.R.
  TITLE     Suppression of IL-1beta promotes beneficial accumulation of
            fibroblast-like cells in atherosclerotic plaques in clonal
            hematopoiesis
  JOURNAL   Nat Cardiovasc Res 3 (1), 60-75 (2024)
   PUBMED   38362011
REFERENCE   6  (residues 1 to 1221)
  AUTHORS   Panagopoulos,I., Mertens,F., Isaksson,M. and Mandahl,N.
  TITLE     Expression of DOL54 is not restricted to myxoid liposarcomas with
            the FUS-DDIT3 chimera but is found in various sarcomas
  JOURNAL   Oncol Rep 12 (1), 107-110 (2004)
   PUBMED   15201968
  REMARK    GeneRIF: Expression is characteristic ofmyxoid liposarcoma and is
            also a frequent finding also in various other sarcomas.
REFERENCE   7  (residues 1 to 1221)
  AUTHORS   Kuznetsov,N.V., Sandblad,L., Hase,M.E., Hunziker,A., Hergt,M. and
            Cordes,V.C.
  TITLE     The evolutionarily conserved single-copy gene for murine Tpr
            encodes one prevalent isoform in somatic cells and lacks paralogs
            in higher eukaryotes
  JOURNAL   Chromosoma 111 (4), 236-255 (2002)
   PUBMED   12424524
REFERENCE   8  (residues 1 to 1221)
  AUTHORS   Koshizuka,Y., Ikegawa,S., Sano,M., Nakamura,K. and Nakamura,Y.
  TITLE     Isolation of novel mouse genes associated with ectopic ossification
            by differential display method using ttw, a mouse model for ectopic
            ossification
  JOURNAL   Cytogenet Cell Genet 94 (3-4), 163-168 (2001)
   PUBMED   11856874
REFERENCE   9  (residues 1 to 1221)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   10 (residues 1 to 1221)
  AUTHORS   Kuroda,M., Wang,X., Sok,J., Yin,Y., Chung,P., Giannotti,J.W.,
            Jacobs,K.A., Fitz,L.J., Murtha-Riel,P., Turner,K.J. and Ron,D.
  TITLE     Induction of a secreted protein by the myxoid liposarcoma oncogene
  JOURNAL   Proc Natl Acad Sci U S A 96 (9), 5025-5030 (1999)
   PUBMED   10220412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161432.5.
            
            On Nov 27, 2007 this sequence version replaced NP_067375.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data because no single transcript was available for the full length
            of the gene. The extent of this transcript is supported by
            orthologous human data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN04095720
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Mus musculus"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 63.84 cM"
     Protein         1..1221
                     /product="proteoglycan 4 isoform 1 precursor"
                     /note="proteoglycan 3; superficial zone proteoglycan;
                     megakaryocyte-stimulating factor"
                     /calculated_mol_wt=131960
     sig_peptide     1..24
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2637
     Region          26..68
                     /region_name="SO"
                     /note="Somatomedin B -like domains; smart00201"
                     /db_xref="CDD:197571"
     Region          69..106
                     /region_name="Somatomedin_B"
                     /note="Somatomedin B domain; pfam01033"
                     /db_xref="CDD:460034"
     Region          <446..761
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <634..938
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          959..1220
                     /region_name="HX"
                     /note="Hemopexin-like repeats.; Hemopexin is a
                     heme-binding protein that transports heme to the liver.
                     Hemopexin-like repeats occur in vitronectin and some
                     matrix metalloproteinases family (matrixins). The HX
                     repeats of some matrixins bind tissue inhibitor of...;
                     cd00094"
                     /db_xref="CDD:238046"
     Site            order(968,970,1012,1014,1060,1062,1168,1170)
                     /site_type="metal-binding"
                     /note="Metal binding sites [ion binding]"
                     /db_xref="CDD:238046"
     CDS             1..1221
                     /gene="Prg4"
                     /gene_synonym="CACP; DOL54; JCAP; lubricin; MSF; SZP"
                     /coded_by="NM_021400.3:41..3706"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS48392.1"
                     /db_xref="GeneID:96875"
                     /db_xref="MGI:MGI:1891344"
ORIGIN      
        1 mgwkilpvcl slllpvvliq qvssqdlssc agrcgegysr datcncdync qhymeccpdf
       61 krvcttelsc kgrcfesfar grecdcdsqc kqygkccady dsfceevhnp tspspktapp
      121 ppgashtiks ttkrspkspk kktkkvvese eiteehsvse nqesssssss ssstirkiks
      181 sknsanrelq knpkvkdnkk ntpkkkpnpe ppavdeagsg ldngefkltp pppdppttph
      241 skvttspktt aakpvtpkps lapnsetske aslasnkett vetkettatn kqssaskkkt
      301 tsaketrsae ktsdkdvept sttpknsapt ttkkpvtttk epvpttpkep epttpkeppp
      361 ttkkpepttp kepepttpke pepttpkepe pttpkepept tpkepppttk kpepttpkep
      421 epttpkepep tttkepeptt tkepesttrk epepttpkep epttpkepep ttpkepeptt
      481 pkepepttlk epepttpkep epttpkepep ttpkepeptt pkepepttpk epepttpkep
      541 epttpkepep ttpkepeptt pkepepttpk epepttpkep epttlkepep ttpkepeptt
      601 pkepepttlk epepttpkep epttlkepep ttpkepeptt tkepesttrk epepttpkep
      661 epttpkepep ttpkepeptt pkepepttpk epepttpkep epttpkepep ttpkkpeptt
      721 pkepepttpk epepttpkep epttpkepep ttrkepeptt pkepepttpk epepttpkkp
      781 eptttspktt tlkattlapk vtapaeeiqn kpeettpase dsddskttlk pqkptkapkp
      841 tkkptkapkk ptstkkpktp ktrkpkttps plkttsatpe lnttplevml ptttipkqtp
      901 npetaevnpd hedadggege kplipgppvl fptaipgtdl lagrlnqgin inpmlsdetn
      961 lcngkpvdgl ttlrngtlva frghyfwmln pfrppspprr itevwgipsp idtvftrcnc
     1021 egktfffkds qywrftndvv dpgypkqivk gfggltgkiv aalsiakykd rpesvyffkr
     1081 ggniqqytyk qepmkkctgr rpainysvyg eaaqvrrrrf eravgpfqth tfrihysvpm
     1141 rvsyqdkgfl hnevkvstmw rgfpnvvtsa itlpnirkpd gydyyafskd qyynidvptr
     1201 taraittrsg qtlskiwync p
//
