LOCUS       NM_029525              11057 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatidylinositol-3,4,5-trisphosphate-dependent Rac
            exchange factor 2 (Prex2), transcript variant 1, mRNA.
ACCESSION   NM_029525 XM_001475275
VERSION     NM_029525.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   Fu,Q., Huang,X., Wan,S., Li,Y., Li,X., Su,S., Xu,X. and Wu,Y.
  TITLE     P-Rex2 mediation of synaptic plasticity contributes to bone cancer
            pain
  JOURNAL   Mol Pain 18, 17448069221076460 (2022)
   PUBMED   35083941
  REMARK    GeneRIF: P-Rex2 mediation of synaptic plasticity contributes to
            bone cancer pain.
REFERENCE   3  (bases 1 to 11057)
  AUTHORS   Lissanu Deribe,Y.
  TITLE     Interplay between PREX2 mutations and the PI3K pathway and its
            effect on epigenetic regulation of gene expression in NRAS-mutant
            melanoma
  JOURNAL   Small GTPases 7 (3), 178-185 (2016)
   PUBMED   27111337
  REMARK    GeneRIF: complex signaling mechanisms that involve PREX2,
            PI3K/AKT/PTEN and downstream epigenetic machinery to deregulate
            expression of key cell cycle regulators
REFERENCE   4  (bases 1 to 11057)
  AUTHORS   Lissanu Deribe,Y., Shi,Y., Rai,K., Nezi,L., Amin,S.B., Wu,C.C.,
            Akdemir,K.C., Mahdavi,M., Peng,Q., Chang,Q.E., Hornigold,K.,
            Arold,S.T., Welch,H.C., Garraway,L.A. and Chin,L.
  TITLE     Truncating PREX2 mutations activate its GEF activity and alter gene
            expression regulation in NRAS-mutant melanoma
  JOURNAL   Proc Natl Acad Sci U S A 113 (9), E1296-E1305 (2016)
   PUBMED   26884185
REFERENCE   5  (bases 1 to 11057)
  AUTHORS   Mense,S.M., Barrows,D., Hodakoski,C., Steinbach,N., Schoenfeld,D.,
            Su,W., Hopkins,B.D., Su,T., Fine,B., Hibshoosh,H. and Parsons,R.
  TITLE     PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor
            cell invasion
  JOURNAL   Sci Signal 8 (370), ra32 (2015)
   PUBMED   25829446
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11057)
  AUTHORS   Jackson,C., Welch,H.C. and Bellamy,T.C.
  TITLE     Control of cerebellar long-term potentiation by P-Rex-family
            guanine-nucleotide exchange factors and phosphoinositide 3-kinase
  JOURNAL   PLoS One 5 (8), e11962 (2010)
   PUBMED   20694145
  REMARK    GeneRIF: PI3K/P-Rex/Rac pathway is required for late phase
            long-term potentiation in the mouse cerebellum
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 11057)
  AUTHORS   Donald,S., Humby,T., Fyfe,I., Segonds-Pichon,A., Walker,S.A.,
            Andrews,S.R., Coadwell,W.J., Emson,P., Wilkinson,L.S. and
            Welch,H.C.
  TITLE     P-Rex2 regulates Purkinje cell dendrite morphology and motor
            coordination
  JOURNAL   Proc Natl Acad Sci U S A 105 (11), 4483-4488 (2008)
   PUBMED   18334636
  REMARK    GeneRIF: P-Rex1 and P-Rex2 are important regulators of Purkinje
            cell morphology and cerebellar function
REFERENCE   8  (bases 1 to 11057)
  AUTHORS   Lickert,H., Cox,B., Wehrle,C., Taketo,M.M., Kemler,R. and
            Rossant,J.
  TITLE     Dissecting Wnt/beta-catenin signaling during gastrulation using RNA
            interference in mouse embryos
  JOURNAL   Development 132 (11), 2599-2609 (2005)
   PUBMED   15857914
REFERENCE   9  (bases 1 to 11057)
  AUTHORS   Donald,S., Hill,K., Lecureuil,C., Barnouin,R., Krugmann,S., John
            Coadwell,W., Andrews,S.R., Walker,S.A., Hawkins,P.T., Stephens,L.R.
            and Welch,H.C.
  TITLE     P-Rex2, a new guanine-nucleotide exchange factor for Rac
  JOURNAL   FEBS Lett 572 (1-3), 172-176 (2004)
   PUBMED   15304343
REFERENCE   10 (bases 1 to 11057)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK138884.1, AC102631.9, AC102481.10, AC164600.5 and AI553603.1.
            
            On Apr 19, 2008 this sequence version replaced XM_001475275.1.
            
            Transcript Variant: This variant (1) represents the longer variant
            and it encodes the longer protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AM109952.1, SRR10223969.48.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3019              AK138884.1         2-3020
            3020-3242           AC102631.9         178719-178941
            3243-3450           AC102631.9         179450-179657
            3451-3630           AC102631.9         190721-190900
            3631-3725           AC102631.9         193312-193406
            3726-3808           AC102631.9         193996-194078
            3809-3899           AC102631.9         194753-194843
            3900-4028           AC102631.9         195503-195631
            4029-4070           AC102631.9         202379-202420
            4071-4288           AC102631.9         208701-208918
            4289-4391           AC102631.9         212932-213034
            4392-4535           AC102631.9         217378-217521
            4536-4650           AC102481.10        49806-49920         c
            4651-4717           AC102481.10        15857-15923         c
            4718-4908           AC102481.10        15443-15633         c
            4909-5011           AC164600.5         66499-66601
            5012-5079           AC164600.5         70695-70762
            5080-11022          AC164600.5         79150-85092
            11023-11057         AI553603.1         1-35                c
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.57 cM"
     gene            1..11057
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /note="phosphatidylinositol-3,4,5-trisphosphate-dependent
                     Rac exchange factor 2"
                     /db_xref="GeneID:109294"
                     /db_xref="MGI:MGI:1923385"
     exon            1..445
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            446..517
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            518..640
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            641..745
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            746..847
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1009
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1143
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1247
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1397
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1542
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1643
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1747
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1797
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1873
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..1946
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2089
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2182
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2331
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2417
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2418..2554
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2672
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2857
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..3019
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3242
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3450
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3630
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..3725
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3726..3808
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3809..3899
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            3900..4028
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4070
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4071..4288
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4289..4391
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4392..4535
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4536..4650
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4651..4717
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4718..4908
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            4909..5011
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            5012..5079
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     exon            5080..11054
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11033..11038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /note="hexamer: AATAAA"
     polyA_site      11054
                     /gene="Prex2"
                     /gene_synonym="6230420N16Rik; C030045D06Rik; D430013K02;
                     Depdc2; P-Rex2"
                     /note="major polyA site"
ORIGIN      
        1 atttgattga tgtccaagtc accgctgtgg ctggagccgc ctcccctcgg gctggagccg
       61 cgtgaggccg gagcagcggg ccgggctggg ttcccgctct ccggggactg tcccgttctg
      121 agtccagcgc gtcccgcgcg tcccgcgctc gcaccgcagc ccgagcacag tcctctgccc
      181 acaccccggc ctccggccag catgtgaagc tacgcgttcc ggctgggact cggcagaggc
      241 ggcaactttg ctggcggcag tggcggcagt ggcggcagca ggcgcggggc agcgcacttg
      301 cccccagctc cgcgccccgc cggccaccat gagcgacgaa agcgccaggg aagtagacaa
      361 gcagcttcgc ctgcgcgtgt gcgtgctcag cgagctgcag aagaccgagc gcgactacgt
      421 gggcacgctg gagttcctgg tgtcggcctt cttgcatcga atgaaccaat gtgcagcagc
      481 aaaggttgat aaaaatgtga cggaggaaac agtgaagatg ttattctcca atatcgagga
      541 gatcctcatc gttcataagg agttcttgaa agttgtagaa gaatgcttat atccggaacc
      601 cagtgctcag caggaagtgg gagcctgctt ccttcacttt aaggacaagt ttcgtatcta
      661 tgatgaatat tgcagcaacc atgaaaaggc acaaaaattg cttcttgaac tgaacaaaat
      721 aagaacaatc cggacatttc ttctgaactg catgctgctt ggaggtcgga agaatacaga
      781 cgttcccctg gaaggatatt tagtgacacc aattcagagg atatgcaagt accctcttct
      841 tttgaaggaa ttactgaagc ggactccacg gagacatagt gactacacag cagtgatgga
      901 agcactccaa gccatgaaag ccgtgtgttc caacattaat gaggccaaga gacagatgga
      961 gaaactggaa gttttagaag agtggcaggc acacattgaa ggctgggagg ggtccaacat
     1021 tactgacacc tgtactgaga tgctgatgtg tggagtccta atgaaaattt cttccggaaa
     1081 tattcaagaa cgagtgtttt ttctttttga taatctttta gtatactgca aaagaaaaca
     1141 cagacggctg aagaatagta aggcatccac agatgggtat cggtatgtct ttcgagggcg
     1201 gatcaatact gaggtgatgg aagtggaaaa tgtggatgac ggcacagctg attttcacag
     1261 cagtgggcat attgttgtga atgggtggaa gatacacaac acagcaaaaa ataaatggtt
     1321 tgtatgcatg gctaaaagcc ctgaagaaaa gcatgagtgg tttgaagcta ttttgaaaga
     1381 aagagaacgt cggaaagggt taaaactagg aatggaacaa gacacctggg taatgatctc
     1441 tgagcagggt gagaaattgt ataaaatgat gtgcaaacag ggaaatctga tcaaagacag
     1501 aaaaagaaaa ctgaccacat ttcctaagtg cttcctggga agtgaatttg tctcatggct
     1561 attggaaatt ggagagattc acaggcccga agaaggtgtt cacttaggac aagcgctgtt
     1621 agagaatggg atcatccacc atgttaccga taagcaccag ttcaaaccgg agcaaatgct
     1681 ataccgattc cgttatgacg atggtacatt ttaccccagg agtgagatgc aggacgtgat
     1741 ttctaagggt gtaaggctct actgtcgtct tcatagtctg tttaccccag tggtcagaga
     1801 taaagattac catttaagaa cttacaaatc tgttgtcatg gccaacaaat tgatagactg
     1861 gttaattgca cagggtgatt gccgtaccag ggaagaggca atgatatttg ctgttgggct
     1921 ctgtgacaat ggatttatgc atcacgtcct agaaaaaagt gaattcaaag atgagcctct
     1981 acttttccgc ttttttgctg atgaggaaat ggaaggatca aatatgaagc ataggcttat
     2041 gaaacatgac ttgaaggttg tggagaatgt catagctaag tcactgttga ttaaatccaa
     2101 cgaaggcagc tatggctttg gcctagaaga caaaaacaaa gttccaataa taaaattagt
     2161 ggaaaagggg tcgaatgccg agatggctgg catggaagtt gggaagaaga tatttgccat
     2221 taatggtgac cttgtttttc tgagaccttt tcctgaagtc gattgcttcc tgaaatcgtg
     2281 cttaaacagc agaaagccac tgagagtcct cgtgagcaca aagccaagag agaccgttaa
     2341 gattccagat tctgcagacg ggcttggttt ccagatcaga ggctttggac cttcagttgt
     2401 gcatgcggtg ggaagaggta ctgtggctgc agcagctggc cttcatccag gacagtgtat
     2461 tatcaaagtg aatggaatca atgtcagcaa agagacacat gccagtgtca tcgcacatgt
     2521 cacagcctgc cggaaataca aacggccaat gaagcaagat tccatacagt gggtctacga
     2581 cagtcttgaa agtgctcagg aagacattca gaagtcccac tcaaagcccc ctggagacgg
     2641 ggctggagat gcttttgagt gcaaagtaga agatgtcatt gacaagttca acaccatggc
     2701 tattattgat ggcaagaagg agcatgtgag tctgacggtg gacaacgtcc acctcgagta
     2761 tggggttgta tatgagtatg atagcacggc gggcacaaag tgcaatgtgg tggagaagat
     2821 ggtggagccc aaagggttct tcagcctaac tgccaagatt ctggaagccc tagcaaaaag
     2881 tgacgaacat tttgtccaaa actgcaccag ccttaattct ctgaatgaag tgatcgccac
     2941 tgatcttcag agtaaattca ccagcatgtg cagtgagaga atcgaacatg tgtgtcacag
     3001 aatatccagc tatgggaggt tctctcgggt gctgaagaac agagcctggc ccacctttaa
     3061 gcaggccaaa cctaagatct ccccactgca cagcagtgat ttctgcccaa ccaactgcca
     3121 cgtcaatgtg atggaagttt cttatccgaa aacatcaact tccctgggga gtgccttcgg
     3181 tgtgcagcta gatagcagaa aacataattc tcacgataaa gaaaataaat ctgtcgagcc
     3241 agggaaactg agccccatgg tgtatattca gcacacaatc acaaccatgg cagcgccttc
     3301 gggcctgtca ctgggacaca aggatgggca tggcctccag tatttgctga aagaagaaga
     3361 cttagaaact caagatatct atcacaaact tctgggcaag ctgcagactg cactgaagga
     3421 ggtagagatg agcgtttgtc aaattgatga ccttctgtct tcaataacat attctcctaa
     3481 attggaacgt aagacaacag agtgtgtgac accaatggac agtgacaacg agaagggaga
     3541 gagaaatagc aaacgtgtct gttttaatgt agcaggggat gagcaagagg attcaggcca
     3601 tgatacagtc agcaacagag actcttacag tgactgcaac agcaacagga attccattgc
     3661 ttccttcacg agtatctgca gcagccagtg cagttcctac ttccacagtg atgagatgga
     3721 ctcaggtgat gagcttccta tcagtgtccg gatatctcat gataaacaag acaagatcca
     3781 cacttgcctg gaacagctct tcagccagat tgattcaata atcaacctct tgaaaggaca
     3841 agctgttata agagcctttg agcagaccaa gtatctcaca ccgggtcgag gattgcaaga
     3901 attccaacag gaaatggagg caaagctgag ttgccccagg aggttgcggc tccatctcaa
     3961 gcaggacccc tggaatcttc ccagcagcat ccaggcgctt gcacagagca tcagaaaaca
     4021 tgctgaagaa gtgaagtgcc gcatactact ggctctcctt gaatactcag acagtgaaac
     4081 ccaactccgg agagacatgg ttttctgtca aagtcttgtg gccactgtct gtgccttctc
     4141 tgagcagctc atggctgcct taaaccagat gtttgacaac agcaaggaaa atgaaatgga
     4201 gacttgtgaa gccagcagga ggtggctgga ccagattgca aatgctggtg tgctttttca
     4261 cttccagtca cttctgtcac caaatttgaa agatgaacaa gccatgcttg aggatacact
     4321 ggttgccctg tttgatttgg aaaaagtttc cttcttcttc aaaccatcag aagaggatcc
     4381 actggttgca aatgtccctc ttacctacca agtggaagga agcaggcagg ctctgaaagt
     4441 ttacttctac atggatagtt accattttga gcagctgccc caacggttga agaatggagg
     4501 agggtttaaa attcatcctg ttctcttttc acaagcattg gagagtatgg aaggatattg
     4561 ctatagagac aacatctctg tggaagagtt tcaagcacaa ataaacacag cctccctgga
     4621 aaaggtcaaa cagtacaatc agaagctcag ggcattctac ttggacaagt caaattcccc
     4681 accgaatacc acatctaaag ctgcctacat agataagctc atgaagcctc tcaatgcttt
     4741 agatgaactt taccggctga tcacctcatt tatcagatcc aaacgcatcg ctgcctgtgt
     4801 gaacacacct tgcagcgcct ctggagtcgg actgctgtca gtttcttcag agctgtgtga
     4861 caggctcggt gcctgtcaca tcatcatgtg cagcagtggt gtgcaccggt gcaccctgag
     4921 tgtgacacta gagcaaacca tcactttagc aagaagccat gggctccctc ctcggtacat
     4981 tatgcaggcc atggatgtga tgagaaagca gggtgcaaga gttcagaaca cagctaaaaa
     5041 cttgggcgtt agagaccgga ctcctcagtc agctccaagg ctgtacaagc tctgcgagcc
     5101 accacctcca gtaggagaag aatgagcatt ccacgtgcca tgggacagat gcttccagat
     5161 gctgcccaaa ttaacattct ccaccgaaga caaagagatg caaattaaag atttaaagtt
     5221 ttcccatgat gtaaataact gatgtttggt ttggacattt atgctggaac tagaagttcc
     5281 ttcagacagt tcagccttac actctataca ttaagaatac agattcacat tagggcaaaa
     5341 ttcacttctc gtatttgtag aaaaatatac agagataaaa gattaactat tttggttcct
     5401 ttctcattca ttaattatat tgtatttgtg tttagcttcc ccaatggaca gatattatac
     5461 ttgatagttt ttcttgaaga agtctgtcct aaaaatgatg accacactct ggaaacaatc
     5521 atacagaaac gttaaggaca gtgtagatga ggagttacag gtgtcccata taccacttga
     5581 gtcaccaaga aaggcgcata tgagaggtag ggaagtacct gcctcccttt cttcttgggc
     5641 ttcctggtaa caaagagagc tcagaggttc tggcaagact ttttcccttt tgttatgtct
     5701 agtctctggt cttgtaaaca ctccagtagg caggtgcttt tcttgatctt tccaatgagc
     5761 catgagtgct gcgtgatgga ccactgatgg cggtgggtag gaagttgcta ggttggagag
     5821 agaggagtga atgaggatgg aaaataatac atgtattgac cactcacccc ttgccagaga
     5881 aagataaatt cttaattata tgaagtctta ctatgaaatg tcagactgtt aaaataacac
     5941 accttcctga gaatttttct atcaagttat atgtgtaaga tatgaaaata gatttatttg
     6001 ttgtacttca aaaggtcatt agagaaaaac tttgataatt tatggaacca aggtatagac
     6061 tgtattcatt ctaaagtata tgcaatattt atcacttctg tgttaatata ctaaaattat
     6121 tttagcacat aaatatatta atattggttt taaatatata tctttacaaa taattaaaac
     6181 agcatcactg tgattatgat agttgtgctt tgtttaatga caggaataaa ttgtgccttt
     6241 aggtgatatt atcatcgtat aaatgtcatg gggtgtactt acacaagctg agatgactgt
     6301 ggcctcagac actatgacct atggaaccac ttcctatagg ctgtgcatca ctggcccaaa
     6361 gacctctaac agcacagaac tgtgtgtgga tatgggctaa gcacatgaat tccttacttt
     6421 aactacaggt ttggcaaacc attattcagc tttactttat aaatctcata atgaatttca
     6481 gactatcatt aaaagctatc attactgcca gtgatatatt gaatttaaaa gtgtgcttca
     6541 aaggtatggg atacatatat cctacaagtt aagagtgaaa attattcatc acagagtaca
     6601 taagattttt cagtaaatct atttgtgact aatcatagag aaaccaacat tagtttctaa
     6661 aaaaaagcct tagcattttg aatttaataa agatatgacc tacgtttgca tatatgtgac
     6721 atacatccat aactttcagg attaatattt ttgctatcat cagcatataa gtgtgaagct
     6781 ctggtagttc aggctgcctt ctctgaaagt cttgagcgta aggagagctg attgcccgac
     6841 taggcagacc cttcacactg ctttactctt ctgtgttcac cagcctcact aagtctccat
     6901 agaggaacag aagggctacg aagcaagctt attttatttt ttctcattaa acttgtttta
     6961 atggtctcaa aattctgtga cagatttttg gtcaagttgt ttccattaaa aaaatactga
     7021 ttttgaaaac taataactta aaactgccat acacacacac acacacacac acacacacac
     7081 acacacacac acacacacac acaaatggtc cacaaaacat tctttccttc tgaaggtttt
     7141 atgattatat tgcctgggca tattatctgc tttttcagta tttataaatt agtgtatttt
     7201 aaggatccag tctatataac agaatgtggt taatgttatc atttttgtta aacatattat
     7261 ttgtgtacac agcacttaca aactataaag cttgctagtg ttttatagta acgtctatca
     7321 ctgtcatatt ttttcatagt aaaatcatag ctgacatggt ttagtgtatt ctaaatggca
     7381 cctttaatta cctagcccac gttttgatat tcctccttca catagccttg tagcagggat
     7441 gaaagctgac cttgtcttat tacctgtact tcacagttct gctaagcaaa tactaaccca
     7501 gtgacttgca caagtccact tgatgaacat gggggagtcc gaagttaatg gttaaaacct
     7561 gtcttaagga ttgcactgcc tttatctgtt gtatgagtac cattataata ttaataatta
     7621 ttttctgact aaagttggag ttttatgaca tgtattgtca tttaatttat atggaagatg
     7681 ttgcaaagtc cagtgggaat atgcagaatg cctttaaagt gcaagaacta ttttcttggc
     7741 attttccatt cataactgaa gaggatccac atgtattgca agccaggagt aggacattat
     7801 ataaaatttg actataattt taaaagtgtt aacacttaaa aacctttgat agatgagtaa
     7861 tcattttcaa taccataata tacacattct tttgacattg gaattttaca ggccttggga
     7921 gtttagaact ttaatgttgc actatcctct gcaactgggg attattgata agctaaggaa
     7981 aacactgctt actgttctta caacttctat atgtggacat agattcctgt ctacccattg
     8041 agagatttca gatcttatgg ggacagaatc atatttacta tcttacagat tttctcattc
     8101 gttttaaaca ttacaaattt aattttgtaa aaatatgaga atttaagggc tgaaaatatg
     8161 agagaaagac agacagagga tgacaatttc tggtccaggt ttacttggca gaatatgtaa
     8221 atgctgggtt tctactgcat gctctctcct ggaaaccaga atcctcatct ttaggagcat
     8281 gctactttct ttccttttaa cagaagtttt ctgatgtctt caatcccctg tgtattcatt
     8341 tggctcctct tcttgctaca agatgttcaa ttaaacagga aggtagagct gggaatgtgg
     8401 cacagtggtt gagtgcttgc ctagcacatg tgaagccatg ttaaaatata ttggctctcc
     8461 tttaaaccaa tggtggctca cacctgtaat cccagtactt ggagagtcag taggattttc
     8521 aggagttcaa gggaagcttg agataaatat gaatgtgagg ccagtctaaa ctacagtggg
     8581 agacatggcc catgaaggaa acaaagatac tacagtgtgt gttacatggg gttggtgcta
     8641 tttctggctc tattaatttt aactttgaat agctcaattc agaaagaaat gaagttgatc
     8701 ctatgttaac tttaatggtg aagaaaatca atgagaaaga aattcaagag gaagtattag
     8761 ctaacttaag caaaaaatat cctgcagtaa gcacatagat tttataggca aggttattta
     8821 aagcaaataa gtttctatct caagctatca agtattctta cacagtccaa gcatctgtag
     8881 tcatcacatc taagcaggtg ttttctcagg catttggatt tctatagaat catagtatta
     8941 aaatttcaaa gtaataacat tgctttttaa gttatttgtc cttttaaaaa aattttgaaa
     9001 gtaattctgg aaatataccc tactggtatt ccagtcaaac tactaaagct ttgtatctga
     9061 ctgaactgct tctaaatttt aggaactttc ttttttaaag tctgttttta tgtgagtgtt
     9121 cctgtgaaac tgaggtctga tgactcttcc ttaagcaatt acaacttcat tagcatacat
     9181 aaggttcaat taaataagaa acactgtata tttgtgtgtt taaagtctta taggagagcc
     9241 tcaggaaaat ctttattctg aagaactagc agggtaaatg taagaggcac cattgcaaga
     9301 atggcctcag catagtagtt ttacaaaatg gccaccattt tactaggtgc tgcaattcca
     9361 tgttggtgat agtggttaat acttggttcc agaatcattg gatactttaa tgatttgcca
     9421 ttgtcactgt tgttaacatt cctgttgctg agcacccctg gcatgtctat taaagtacag
     9481 ttagtctgtg tgactctcag tggtgaccta gtgagaaatc tgactcactc agaacagttt
     9541 attctcttaa tgggttaggt tctgaaatct gctaagtctg ctaatgatat acagatgtga
     9601 gtgtgtatgt gtgtgtatgt tctcttataa cataaaccac tttttagttt tgtaaacatt
     9661 aataataaac attagatttt ggtttcttca ttgtatggaa atatgagaca tgtattttat
     9721 agatgaacta tatgatgtct gctgtaaaca aaggtgctgc ctgtgtcatg ggtggacttg
     9781 ttggctgtcc tttgttcact gatgttcatc tgttatttgt atgatccagt ttggtttctg
     9841 atttgtcttg tttgtcatca ctgtgacaaa tcatctgaga taattaagtt acaaagacaa
     9901 aaggtttatt ttggctcaaa gtgttggaga ttctattcca caatcaactg aagtctgtgg
     9961 ttgggcctgt catgaagtag caagtagtgt catgacagag gaagagagca ggacacaact
    10021 cctttgctca ttagccacat cacaaagtaa gagacattgg ccccacagtc tttcaaaggc
    10081 agccttcaac agacacagaa ccacccattg gcctccatct ttcagatgct ttaccacctc
    10141 ccaatagcac tattctggag atcaagccct taatacaggg acctttggga gacacaccag
    10201 ggtccaaact aaatcactat ccagtcaatc tcaaatctgc ttttctggtt taccattaag
    10261 aattagctta cagtggacat aattgctgaa tttttaaatg attgttacta gaaatactgg
    10321 taataacaga aataacaaag attgggaaat taattttagg cacagacaat gataactatt
    10381 atccattcat ataatcagca aatgttttga aattcactta ataagactca accagaattg
    10441 agtatattgg atagatttaa ataaaaaaga ttgtcatagg gtctctgtat tcctatatat
    10501 acaaaggtaa cttgtatctt ttacttaaaa attatattat taacaaggta aatatatcct
    10561 aatactatgt gtttattata tactatatag tcaagaatac taagttgtag aaatatttag
    10621 aaagcatacc tttgaattta agtatctaat gcctcaagca aatctgctag tttcagaaga
    10681 atatatattt ttgtaactgc aagaatatga gtagatcata gcttttgctt aaaatatttt
    10741 tgtaagcatt catttacaca tactcatttt aatagtaaaa tattaaactg attctgtaaa
    10801 agaacctgtt ttgactatgt ctatatttaa tattatgtaa tatccaaaat tcaataaatt
    10861 tcatctgaat gtattattag cctttttttc ctttgtaaag tagccatgtt acatagtttc
    10921 aatcattcca tagtttgtgt atggttaaat tatctaaagt tcattatgta ttttatgtca
    10981 tttgtttttc cccacataaa agattgtaaa agatctgata aatgtcaata ataataaagt
    11041 gaattataga atgaaaa
//
