LOCUS       NM_016703               5471 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Mus musculus prolactin regulatory element binding (Preb),
            transcript variant 1, mRNA.
ACCESSION   NM_016703
VERSION     NM_016703.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5471)
  AUTHORS   Park,J.M., Kim,M.Y., Kim,T.H., Min,D.K., Yang,G.E. and Ahn,Y.H.
  TITLE     Prolactin regulatory element-binding (PREB) protein regulates
            hepatic glucose homeostasis
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1864 (6 Pt A), 2097-2107 (2018)
   PUBMED   29601978
  REMARK    GeneRIF: Glucose, insulin, and pyruvate tolerance were impaired in
            normal mice knocked down for Preb indicating Preb to be a negative
            regulator of liver gluconeogenic genes.
REFERENCE   4  (bases 1 to 5471)
  AUTHORS   Chu,V.T., Graf,R., Wirtz,T., Weber,T., Favret,J., Li,X., Petsch,K.,
            Tran,N.T., Sieweke,M.H., Berek,C., Kuhn,R. and Rajewsky,K.
  TITLE     Efficient CRISPR-mediated mutagenesis in primary immune cells using
            CrispRGold and a C57BL/6 Cas9 transgenic mouse line
  JOURNAL   Proc Natl Acad Sci U S A 113 (44), 12514-12519 (2016)
   PUBMED   27729526
REFERENCE   5  (bases 1 to 5471)
  AUTHORS   Hutchins,J.R., Toyoda,Y., Hegemann,B., Poser,I., Heriche,J.K.,
            Sykora,M.M., Augsburg,M., Hudecz,O., Buschhorn,B.A., Bulkescher,J.,
            Conrad,C., Comartin,D., Schleiffer,A., Sarov,M., Pozniakovsky,A.,
            Slabicki,M.M., Schloissnig,S., Steinmacher,I., Leuschner,M.,
            Ssykor,A., Lawo,S., Pelletier,L., Stark,H., Nasmyth,K.,
            Ellenberg,J., Durbin,R., Buchholz,F., Mechtler,K., Hyman,A.A. and
            Peters,J.M.
  TITLE     Systematic analysis of human protein complexes identifies
            chromosome segregation proteins
  JOURNAL   Science 328 (5978), 593-599 (2010)
   PUBMED   20360068
REFERENCE   6  (bases 1 to 5471)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   7  (bases 1 to 5471)
  AUTHORS   Edgar,A.J.
  TITLE     The gene structure and expression of human ABHD1: overlapping
            polyadenylation signal sequence with Sec12
  JOURNAL   BMC Genomics 4 (1), 18 (2003)
   PUBMED   12735795
REFERENCE   8  (bases 1 to 5471)
  AUTHORS   Weissman,J.T., Plutner,H. and Balch,W.E.
  TITLE     The mammalian guanine nucleotide exchange factor mSec12 is
            essential for activation of the Sar1 GTPase directing endoplasmic
            reticulum export
  JOURNAL   Traffic 2 (7), 465-475 (2001)
   PUBMED   11422940
REFERENCE   9  (bases 1 to 5471)
  AUTHORS   Taylor Clelland,C.L., Levy,B., McKie,J.M., Duncan,A.M.,
            Hirschhorn,K. and Bancroft,C.
  TITLE     Cloning and characterization of human PREB; a gene that maps to a
            genomic region associated with trisomy 2p syndrome
  JOURNAL   Mamm Genome 11 (8), 675-681 (2000)
   PUBMED   10920239
REFERENCE   10 (bases 1 to 5471)
  AUTHORS   Taylor Clelland,C.L., Craciun,L., Bancroft,C. and Lufkin,T.
  TITLE     Mapping and developmental expression analysis of the WD-repeat gene
            Preb
  JOURNAL   Genomics 63 (3), 391-399 (2000)
   PUBMED   10704286
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ145010.1, AK151288.1, AK013330.1, AK029838.1 and BP765513.1.
            
            On Jun 28, 2014 this sequence version replaced NM_016703.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encode the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029838.1, AK036866.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                CJ145010.1         4-22
            20-1735             AK151288.1         2-1717
            1736-1841           AK013330.1         1530-1635
            1842-4788           AK029838.1         1778-4724
            4789-5471           BP765513.1         1-683               c
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 16.9 cM"
     gene            1..5471
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /note="prolactin regulatory element binding"
                     /db_xref="GeneID:50907"
                     /db_xref="MGI:MGI:1355326"
     exon            1..357
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..1476
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prolactin regulatory element-binding protein; mammalian
                     guanine nucleotide exchange factor mSec12; mammalian
                     guanine nucleotide exchange factor Sec12; guanine
                     nucleotide-exchange factor SEC12"
                     /codon_start=1
                     /product="guanine nucleotide-exchange factor SEC12 isoform
                     1"
                     /protein_id="NP_057912.2"
                     /db_xref="CCDS:CCDS39051.1"
                     /db_xref="GeneID:50907"
                     /db_xref="MGI:MGI:1355326"
                     /translation="MGRRRGVELYRAPFPLYALRIDPKTGLLIAAGGGGAAKTGIKNG
                     VHFLQLELINGCLSASLLHSHDTETRATMNLALAGDILAAGQDAQCQLLRFQVHQQKG
                     SKAEKSGSKEQGPRQRKGAPPAEKKSGAQVHPEGVELKVKNLEAVQTDFSNEPLQKVV
                     CFNHDNTLLATGGTDGHVRVWKVPSLEKVLEFKAHEGEIGDLTLGPDGKLVTVGWDFK
                     ASVWQKDQLVTQLQWQENGPASSNTPYRYQACRFGQVPDQLGGLRLFTVQIPHKRLRQ
                     PPPCYLTAWDSSTFLPLRTRSCGHEVISCLSVSDSGTFLGLGTVTGSVAIYIAFSLQR
                     LYYVKEAHGIVVTDVTFLPEKGCGPKLLGPHETALFSVAVDSRCQLHLLPSRRSVPVW
                     LLLLLCVGLIIVTILLLQTAFPGFL"
     exon            358..547
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            548..768
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            769..849
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            850..974
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1148
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1221
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1381
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..5452
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2051..2056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /note="hexamer: AATAAA"
     polyA_site      2077
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /note="major polyA site"
     regulatory      5427..5432
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Preb"
                     /gene_synonym="mSec12"
                     /note="hexamer: CATAAA"
     polyA_site      5448
                     /gene="Preb"
                     /gene_synonym="mSec12"
ORIGIN      
        1 gagagcccag gagctcggcg cgtgcgcact gaacgcctgc aagacccagt cggccgcgcg
       61 ggcgcagatc ggctgcttgc ggtggtttga gtggtggcaa gtccagtgct gagaggggtc
      121 ggcagtcttc cggaaaactc tagcgccaag ttcctgagct ttcggaggcg aggcgcggca
      181 tgtcgtgggt tccgcggggt tggcggtgaa cgtgcgggcg ggatgggtcg gcgccggggt
      241 gtggagctgt accgggcccc gttcccgttg tacgcgcttc ggatagaccc caagactggg
      301 ctgctcatcg ctgcgggcgg aggaggagct gccaagaccg gcataaagaa tggcgtgcat
      361 tttctgcagc tagagctgat caacgggtgc ctgagcgctt ccttgctgca ctctcatgac
      421 acggagacac gggccaccat gaatttggcg cttgctggtg acattcttgc tgccggacag
      481 gatgcccagt gtcagcttct tcgtttccag gtccatcaac agaagggcag taaagcggag
      541 aagtcaggtt ccaaggagca gggacctcga cagagaaagg gggctcctcc agcagagaag
      601 aaatcgggag cacaagttca ccccgaaggg gttgaactca aagtaaagaa tttggaggca
      661 gtacagacag acttcagcaa tgaaccgctg cagaaagttg tgtgcttcaa ccatgataac
      721 accctgcttg ccactggagg aactgatggt catgttcgtg tctggaaggt acctagcctg
      781 gagaaagttc tggagttcaa agcccatgaa ggggagattg gagatttgac tttgggtcct
      841 gatggcaagc tggttacagt gggctgggac tttaaggcct ctgtgtggca gaaggatcaa
      901 ctggtgacac agctacagtg gcaagagaat ggaccagcct cttctaatac accataccgc
      961 taccaggcct gcaggtttgg gcaggttcca gatcagctcg gtgggctgcg actcttcaca
     1021 gtgcagatac cccacaagcg cctacgtcag cccccaccct gctacctcac agcctgggac
     1081 agttccacct tcttgcctct tcggaccaga tcctgtggcc atgaggtcat ttcctgcctc
     1141 agtgtcagtg attcgggtac cttcctaggc ctaggcacgg tcactggctc tgtcgccatc
     1201 tacatagctt tctctctcca gcgcctgtat tatgtgaagg aggcccatgg cattgtggta
     1261 acagatgtga cctttctacc tgagaagggt tgtggtccaa agctccttgg gccccatgaa
     1321 actgctcttt tctctgtggc tgtggatagt cgttgccagt tgcacctgct gccttcacgg
     1381 cggagtgttc ctgtatggct tctgctcctg ctgtgtgtcg gccttattat tgtgaccatc
     1441 ctgctgctcc agactgcctt cccgggattt ctctaacctc tagagcgatg ggaatcagcc
     1501 ttggacagtg ccgccttcca gagcagaatc actgagcccc acggctgaag ctgcatctga
     1561 tgaaactgat gggtactgct tgtccctcag ctcacacttg gcggtggcct ctcgatcact
     1621 ctgggtcttg ggagctcttg ccttcacctg tggatccact taagacattg tggtctgaag
     1681 ctcaggccac agtgcctgcc tcgttccctc agcctccctc ccagggctcc agagctcagc
     1741 tcttcctttt ctagaagcat gtgaagatgc ccaagagcct ggagacattg ccatccttcc
     1801 tacagaaact gtttctcctc ctcccctcca gccttcacac aagggccttg gctttgttga
     1861 gacagagcct gtgctggcac ttgctgttag ggtaggaaga ggcacaaaga ggaccccggt
     1921 agcggtgatc tgtggccttc tgtgggtagc accaagccag tcagtccaga cacatagtga
     1981 gttcctggag ggtctactgc agcctgagga aagggaacct cagctcgtta ggcaggaaga
     2041 gttgatattt aataaagaga gaacaattga accgagagcc cagcagtcct cttgtcagct
     2101 ctttccatct tcaggagtga gtactcccag gtcaggtagc cccactgagt gctggaagat
     2161 ggctcgggca tactgatgta gtacgcggtt catgtaacag tgttgccagg gcatcagccc
     2221 ttccaggaag ccaatgtggc caccccgagc tgtgatgagc agggccacat agggagactt
     2281 ctgggcagcc tgcagaggga aggctagaag ggagtcagga ggtcagaaag ctgtccggga
     2341 atgagaaccc aggctcatac ctcaaccctc atctatggct cagtagctgt gggatcagga
     2401 cctcgttgca ggcactcacc atggactggg gagaaggggt catctgctgc attgaggcag
     2461 agaacagggg tgtggatggc atctaccttg gttcttgggc ttgcggcttg gtaataagcc
     2521 gcacagtctt tatacccaaa agccacagat gtgtagcgct catccagctg gcggatcgtg
     2581 cgggcctgtg tggtagaggc atggtaggct ataatattag aacgtaggag accaacactg
     2641 gggtgagagg gtgatcgaaa gataccttta tcgcaaagtc tacatccaac accttttcaa
     2701 ttggctttcg gttcctggaa aagaatacat cagggtttgt ctgaactcct gcacctaacc
     2761 cagagctcac agcttctgtc ttcttacctc ctcaagaaag cccagattgg aatggaccca
     2821 agcactggca gagcctgcca tctgctatcc tacctggcca caagccggca gagtccagca
     2881 gtgagtggct gattgaagag cagtgagttg agtggggtct ccaaggagtc aacggtctca
     2941 aaggaatccc aacatgcaga caatggtcag tcctgcgacc aagcctccag ccttcccagt
     3001 ctgtgccaga tagttaagca ccagtatcct gtaaaattgg atgcagggcc atggaaagtg
     3061 ttggcatggg aacactggga cagaagagga gaaaaagaca agatgaacaa acccaggcaa
     3121 ctattaccct ccaaaagaga tgcccacagc cagcagtgga gcttgggagt agcgacgttt
     3181 tatgtgcttc acaactgttt ctaggtcttc agtattgctg gcacagtaag ccctgtgagt
     3241 ctgagggcaa agcgggtcat tgactaggga gggaggctct tgccagaaga cagccaagcg
     3301 gaagggctca ggaagggtag ggggttggta agtaggtaga gccctagggc cagtggtctc
     3361 taaagggaac agggacaggg ccaccacaaa ggaggagggt actcaccagc agctcttccc
     3421 ctcggcatcc tcggttatta aatacaacag ccctggggca agataggagt gtgagccaag
     3481 ccctcccaag aagaccctcc ccttgatagt cttcagaaag gcccctgtca gagagtacca
     3541 tatcagggtc agatgtactt ttgcccttcc caagttctgt accctgtcct tacctatagc
     3601 catccttcag agcttggtta actaggtgta agatgtacgg ctcctggcta ctgccgctga
     3661 tgcctggaag cagcagtact atgggctggg tggtagggtc cgggtagtga gtgctgttag
     3721 gctgctcggc ccagtctaga aggaactggc ctccatctgg ggtctggagg acttcactgg
     3781 ggtttagaga ttgcaacaca aagatgttat taggagttgc acttctgcat gtcaaagctc
     3841 taccctctga ctcgcccaca agctctgctg tttgtcatgg gtatcacttg aaggcacgct
     3901 tcatataaaa cagcaaggtg accagctaga ttggactatt aaccttgtgt gggtgcaagt
     3961 agagagcctt tccatacata taggctcatg ctttgggaca aagattaccg tcatataaag
     4021 aggtggacca tagagacttg cagctttgtg ttcctggtcc tgacccttct tacctccggt
     4081 aagggaccac aggctgagac tgcaggagga ctcggaagat ggtttgcagc cgtccctcaa
     4141 agcaccacag tgtcgggtag aaagtctcca cagtgactgg acagtgttgt tccaggaagg
     4201 ccagaaactg gggcccagcc accagacgag gcctctataa tcatacaaca cattggcctc
     4261 ttttgttgtc ctgtcccaga cactggaggg tcagtgagct cctccccgga atccagcctt
     4321 aaggatggat ttgccacagt caagtgtcca gcacagctca gcctagagtg gcatatggtg
     4381 ccccacaaaa accacagtcc taaagtgtcc cctccgggac tgaccctgaa ggaaggactg
     4441 cagcagttgt cgaggtactg actcccacac tcagcctgtc ccctcctcaa acccacttgt
     4501 gctagaacag gtactataag ccgctcaggt gagcagctag agtatggcct gtgctcctct
     4561 ggctggtaaa ctggagtttt tgtctggttt ggttttattt ctaattatgt gcctctccta
     4621 tgagcatggg cacacaggtc agatcttgcc tgtgagatcc agaagaagtg ttggatcctc
     4681 tggagctgga gttagaggta gctgagagag ccacagcatg gaagctggga caaactgatc
     4741 tgcagggaca gaagtatatg ctcttcaggg ctgagacatc tggcctggat ggttggagcc
     4801 ttgaactcat cgtaatcctc ctgcctctgt gctaggcttg tgtcaccttg gtgtgctggt
     4861 tgggactctt gaccaggtga agatacctct gccattcaat ctggcctttg cattgtagca
     4921 accttgtcca aatgcatcac cctgaagctc attcgggtca ctccagctcc tgtaccccac
     4981 tgattttagc accccagtgt ctacatattt ctctaatgta tgtgtgctca gtgctgaagg
     5041 gcttctttta caggggcacc tcatattctc ttgtggagcc ccagttctta ggctgtgttc
     5101 tacaggagga agcaccccaa cgtgaacaag tatttccttt cctcgtgcca actagagaaa
     5161 agggcacagt gatgggacaa tggggacaaa cgaggttgat gggcaccaag ccctgactct
     5221 cttctgcctc ttcgcttggt tctttattgg catgaagtaa atgagctggc ctagcctcac
     5281 agacctaagg acactgggct gaacagagtt ggaggacgcc agcctaagag cagaatggga
     5341 gggttatgtt gggatacagc aagcccggga cagtctccag cattggagaa ggttgtttgt
     5401 ttgtttgttt gtttgtttgt tttaatcata aattgcactt gtaagaaact taaaaaaaaa
     5461 aaaaaaaaaa a
//
