LOCUS       NM_027504               8605 bp    mRNA    linear   ROD 31-OCT-2023
DEFINITION  Mus musculus PR domain containing 16 (Prdm16), transcript variant
            1, mRNA.
ACCESSION   NM_027504
VERSION     NM_027504.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Hu T, Wu Q, Yao Q, Yu J, Jiang K, Wan Y and Tang Q.
  TITLE     PRDM16 exerts critical role in myocardial metabolism and energetics
            in type 2 diabetes induced cardiomyopathy
  JOURNAL   Metabolism 146, 155658 (2023)
   PUBMED   37433344
  REMARK    GeneRIF: PRDM16 exerts critical role in myocardial metabolism and
            energetics in type 2 diabetes induced cardiomyopathy.
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Kramer RJ, Fatahian AN, Chan A, Mortenson J, Osher J, Sun B, Parker
            LE, Rosamilia MB, Potter KB, Moore K, Atkins SL, Rosenfeld JA,
            Birjiniuk A, Jones E, Howard TS, Kim JJ, Scott DA, Lalani S,
            Rouzbehani OMT, Kaplan S, Hathaway MA, Cohen JL, Asaki SY, Martinez
            HR, Boudina S and Landstrom AP.
  TITLE     PRDM16 Deletion Is Associated With Sex-dependent Cardiomyopathy and
            Cardiac Mortality: A Translational, Multi-Institutional Cohort
            Study
  JOURNAL   Circ Genom Precis Med 16 (4), 390-400 (2023)
   PUBMED   37395136
  REMARK    GeneRIF: PRDM16 Deletion Is Associated With Sex-dependent
            Cardiomyopathy and Cardiac Mortality: A Translational,
            Multi-Institutional Cohort Study.
REFERENCE   3  (bases 1 to 8605)
  AUTHORS   Zhao S, Nie T, Li L, Long Q, Gu P, Zhang Y, Sun W, Lin Z, Liu Q, Qi
            Y, Wang W, Xie M, Loomes K, Cai C, Wu D and Hui HX.
  TITLE     Androgen Receptor is a Negative Regulator of PRDM16 in Beige
            Adipocyte
  JOURNAL   Adv Sci (Weinh) 10 (21), e2300070 (2023)
   PUBMED   37211698
  REMARK    GeneRIF: Androgen Receptor is a Negative Regulator of PRDM16 in
            Beige Adipocyte.
REFERENCE   4  (bases 1 to 8605)
  AUTHORS   Park A, Kim KE, Park I, Lee SH, Park KY, Jung M, Li X, Sleiman MB,
            Lee SJ, Kim DS, Kim J, Lim DS, Woo EJ, Lee EW, Han BS, Oh KJ, Lee
            SC, Auwerx J, Mun JY, Rhee HW, Kim WK, Bae KH and Suh JM.
  TITLE     Mitochondrial matrix protein LETMD1 maintains thermogenic capacity
            of brown adipose tissue in male mice
  JOURNAL   Nat Commun 14 (1), 3746 (2023)
   PUBMED   37353518
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8605)
  AUTHORS   Thompson M, Sakabe M, Verba M, Hao J, Meadows SM, Lu QR and Xin M.
  TITLE     PRDM16 regulates arterial development and vascular integrity
  JOURNAL   Front Physiol 14, 1165379 (2023)
   PUBMED   37324380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8605)
  AUTHORS   Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier
            G, Langin D and Spiegelman BM.
  TITLE     Transcriptional control of brown fat determination by PRDM16
  JOURNAL   Cell Metab 6 (1), 38-54 (2007)
   PUBMED   17618855
REFERENCE   7  (bases 1 to 8605)
  AUTHORS   Warner DR, Horn KH, Mudd L, Webb CL, Greene RM and Pisano MM.
  TITLE     PRDM16/MEL1: a novel Smad binding protein expressed in murine
            embryonic orofacial tissue
  JOURNAL   Biochim Biophys Acta 1773 (6), 814-820 (2007)
   PUBMED   17467076
  REMARK    GeneRIF: PRDM16/MEL1 is a Smad binding protein that may be
            important for development of orofacial structures through
            modulation of the TGFbeta signaling pathway.
REFERENCE   8  (bases 1 to 8605)
  AUTHORS   Du Y, Jenkins NA and Copeland NG.
  TITLE     Insertional mutagenesis identifies genes that promote the
            immortalization of primary bone marrow progenitor cells
  JOURNAL   Blood 106 (12), 3932-3939 (2005)
   PUBMED   16109773
REFERENCE   9  (bases 1 to 8605)
  AUTHORS   Nishikata I, Sasaki H, Iga M, Tateno Y, Imayoshi S, Asou N,
            Nakamura T and Morishita K.
  TITLE     A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
            expressed mainly in t(1;3)(p36;q21)-positive AML and blocks
            G-CSF-induced myeloid differentiation
  JOURNAL   Blood 102 (9), 3323-3332 (2003)
   PUBMED   12816872
REFERENCE   10 (bases 1 to 8605)
  AUTHORS   Herron BJ, Lu W, Rao C, Liu S, Peters H, Bronson RT, Justice MJ,
            McDonald JD and Beier DR.
  TITLE     Efficient generation and mapping of recessive developmental
            mutations using ENU mutagenesis
  JOURNAL   Nat Genet 30 (2), 185-189 (2002)
   PUBMED   11818962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY739066.1, BC059838.1, AB078338.1 and AL627123.13.
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_027504.4.
            
            On Jan 25, 2007 this sequence version replaced NM_027504.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-544               BY739066.1         4-547
            545-2720            BC059838.1         526-2701
            2721-4089           AB078338.1         2613-3981
            4090-8605           AL627123.13        149763-154278       c
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.96 cM"
     gene            1..8605
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /note="PR domain containing 16"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
     exon            1..151
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3942
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /EC_number="2.1.1.367"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transcription factor MEL1; MDS1/EVI1-like gene 1; PR
                     domain-containing protein 16; PR domain zinc finger
                     protein 16; line 27; histone-lysine N-methyltransferase
                     PRDM16"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase PRDM16
                     isoform 1"
                     /protein_id="NP_081780.3"
                     /db_xref="CCDS:CCDS38989.1"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
                     /translation="MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILS
                     PIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKR
                     KMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKQISEDLGSEKFCVDA
                     NQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLG
                     VMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVG
                     SDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDS
                     GKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTV
                     KPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKN
                     HYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAF
                     PALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNS
                     SQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGS
                     DLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQ
                     LLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYP
                     FTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSS
                     GEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHY
                     AKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAM
                     KADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRT
                     HTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH
                     EHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQA
                     STRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPT
                     PEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEA
                     FEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGP
                     SSESGAFNPINHL"
     exon            152..501
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..549
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            550..687
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            688..790
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            791..1001
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1149
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1303
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..2720
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2805
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2975
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2976..3053
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3223
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3398
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3632
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3633..3807
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3808..8605
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8586..8591
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
     polyA_site      8605
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
ORIGIN      
        1 gtgctctgtg cgcatgtgcg aaggtgtcca aactgacaat gctagggaga tgaagatagt
       61 gtgtagctgc ttctgggctc aaggaggagg agagagattc cgcgagccga caccatgcga
      121 tccaaggcga gggcgaggaa gctagccaaa agtgacggtg acgttgtaaa taatatgtat
      181 gaacctgacc cggacctgct ggccggccag agtgccgagg aggagaccga agacggcatc
      241 ctgtccccca tccccatggg gccaccgtcc cccttcccca ccagcgagga cttcactccc
      301 aaggagggct cgccctatga ggctcctgtc tacattcctg aagacattcc aatcccacca
      361 gacttcgagc tacgagagtc ctccatacca ggagctggcc tggggatctg ggccaagcgg
      421 aagatggaaa tcggggagag gtttggcccc tacgtggtga cgccccgggc cgcactgaag
      481 gaggccgact ttggatggga gatgctgacg gatacagagg tgtcatccca ggagagctgc
      541 atcaaaaagc agatctctga agacttgggt agcgagaagt tctgcgtgga tgccaatcag
      601 gcggggtctg gcagctggct caagtacatc cgtgtagcgt gttcctgtga tgaccaaaac
      661 ctcgccatgt gtcagatcaa cgaacagatt tactataaag tcattaagga catcgagcct
      721 ggagaggaac tgttggtgca tgtgaaagaa ggtgcctact ccttgggtgt catggccccc
      781 agcttggatg aggaccccac attccgctgt gatgagtgtg atgagctctt ccagtgcagg
      841 ctggacctga ggcgccacaa gaagtacgcg tgcagctctg caggagccca gctctacgag
      901 ggcctagggg aggaactcaa gcccgagggc cttggcgtgg gcagcgacgg gcaagcgcat
      961 gagtgcaagg attgcgagcg gatgttcccc aacaagtaca gcttggagca acacatgatc
     1021 gtccacacgg aagagcgtga gtacaaatgt gaccagtgtc ccaaggcctt caactggaag
     1081 tccaacctca tccgccacca gatgtctcac gacagtggca agcgcttcga atgtgaaaac
     1141 tgtgtcaagg tgttcacgga ccccagcaac ctccagcgtc acatccgctc acagcatgtc
     1201 ggtgcccggg cccatgcctg ccctgactgt ggcaagacct tcgccacatc ctctggcctc
     1261 aaacagcaca agcatatcca cagcacggtg aagccattca tatgcgaggt ctgccacaag
     1321 tcctacacgc agttctccaa cctgtgccgg cacaagcgga tgcacgccga ctgcaggacg
     1381 cagatcaagt gcaaggactg tgggcagatg ttcagcacta cctcctccct caacaagcat
     1441 cggagattct gcgagggcaa gaaccattac acgcctggca gcatcttcac cccaggcctg
     1501 cccttgaccc ccagccccat gatggacaag acaaaaccct ccccgaccct caaccacggg
     1561 ggcctaggct tcagcgagta cttcccctcc agacctcatc ctgggagcct gcccttctcg
     1621 gctgctcctc cggccttccc cgcactcact ccgggcttcc cgggcatctt tcctccatcc
     1681 ctgtacccac gaccacctct gctacctccc acgccgctgc tcaagagccc cctgaaccac
     1741 gcgcaggacg ccaagctacc cagcccgctg ggaaacccag ccctgcccct tgtctccgcg
     1801 gtcagcaata gcagccaggg tgccacagcg gccaccgggt cagaggagaa atttgatggc
     1861 cgcttggaag acgcatatgc ggagaaggtc aaaaatagga gccctgacat gtcggatggc
     1921 agtgactttg aggatatcaa caccacgacc gggacagact tggacactac cacgggcacg
     1981 gggtcagacc tggacagcga cctggacagt gacagagaca aaggcaagga caaggggaag
     2041 ccagtggaga gcaaacctga gtttgggggt gcatctgtgc cccctggggc catgaacagt
     2101 gtggccgagg taccggcctt ctactcacag cattccttct tcccgccacc cgaggaacag
     2161 ctgctgacgg cctcgggagc tgccggcgac tccatcaagg ccatcgcgtc catcgcggag
     2221 aaatacttcg gtcctggctt catgagcatg caggagaaga agctgggctc actaccctac
     2281 cactccgtgt tccccttcca gttcctgcct aactttcccc actccctcta cccctttacg
     2341 gaccgagccc tcgcccacaa cttgctggtc aaggctgagc caaagtcacc ccgggatgcc
     2401 ctcaaggtgg gcggccccag tgcggagtgc cccttcgacc tcaccaccaa accaaaagag
     2461 gccaaacccg ccctgctcgc acccaaggtc cccctcatcc cctcatctgg cgaggaacag
     2521 ccactggacc tgagcatcgg cagcagggcc agggcaagcc agaacggagg tggccgtgag
     2581 ccgcggaaga accacgtcta cggtgaacgg aagccggggg tcagcgaggg gctgcctaag
     2641 gtgtgcccag cacagctgcc ccagcagccc tccttgcatt atgctaagcc ttcaccgttc
     2701 ttcatggatc ccatctacag ggtagaaaag cggaaggtgg cagaccctgt gggagtcctg
     2761 aaagagaagt acctgcggcc gtccccactt ctgttccacc cccagatgtc agccatagaa
     2821 accatgacgg agaagctgga gagctttgca gccatgaagg ccgactcagg cagctccctg
     2881 cagcccctgc ctcaccaccc gttcaacttc cgctccccac ccccaacgct ctcggatccc
     2941 atcctcagga aggggaagga gagatacacg tgcaggtact gtggcaagat cttccccaga
     3001 tctgcaaatc tcacaagaca tctgaggaca cacacagggg agcagccata caggtgcaag
     3061 tactgtgacc ggtcattcag catctcctcc aacctccagc ggcacgtgag gaacatccac
     3121 aacaaagaga agccgttcaa gtgccatctg tgcaaccgct gcttcgggca gcagaccaac
     3181 ctagaccggc acctgaagaa gcacgaacac gagggcgcac cagtgagcca gcactccggg
     3241 gtgctcacga accacctggg caccagcgcc tcctccccca cctccgagtc ggacaaccat
     3301 gcacttttag atgagaagga agattcttac ttctccgaga tccgaaactt catcgccaac
     3361 agcgagatga accaggcatc cactcgaatg gacaaacggc ctgagatcca agacctggac
     3421 agcaacccac cgtgtccagg ctcagccagt gcaaagccag aggacgtaga ggaggaggaa
     3481 gaggaggagc tggaggaaga ggatgatgac agcttagccg ggaagtcaca ggaggacacg
     3541 gtgtccccca cacctgagcc ccaaggagtc tatgaagatg aagaggatga ggaaccaccc
     3601 agcctgacca tgggctttga ccatacccgg aggtgtgttg aggagcgagg aggcggcctg
     3661 ttagctttgg agccgacgcc gacctttggg aaggggctgg atctccgcag agcagctgag
     3721 gaagcatttg aagttaaaga tgtgcttaat tccaccttag attctgaggt tttaaaacaa
     3781 accctgtaca ggcaggctaa gaaccaggca tatgcaatga tgctgtccct ctctgaagac
     3841 actcctctcc acgccccctc ccagagctca ctggatgctt ggttgaacat cacaggaccc
     3901 tcgtcagagt ccggagcctt taaccccatc aaccacctct gaggtcctgg aggccccagg
     3961 gccagagtcc aaagccaggg taccagctgc aggagatgga gaaggggcca gggagcagcc
     4021 ccccaccctc aacacctcca ctttgcaaag tccagcttct ccattgaaac tcagaacccg
     4081 aaggtccctt gagtagccgc tggccttcat cacctctcag aactggcctc aaggacacga
     4141 tctgcagtgg gtgcggtgca cgggccaccc aggagctgct cacaggagcc atggatcaga
     4201 aaactcgtgg gcaagggtgg ggtctctatc ccagcaggag ccagttggcc acatccaggc
     4261 aactgcatgg tatgaaagag gaaatcggaa agacgtgggc aagtgctatg gagagagacc
     4321 tcatcaatga tttttataat gagaatcaca tgattaagcc ttttggtaat cttattgact
     4381 atagagtcta tttaagcatg tgggttttaa aaaaaataga cggtattttt taaaaatcaa
     4441 aaaaaaaaaa aaaagacttg caaaatgttt ttttaaaagt aattttgcat cgctttggaa
     4501 tttcagctta ttggcaaacc cacacctgcc aggacatcga gtacggctcg ggtgtgccct
     4561 ttataatgta aataatggag aatttttttt ctatctctga cctgtttgta taagccaagg
     4621 tgattccagt gtgcccgaca caggataagg ggcatggagc cactctgcta agtcagatgc
     4681 tgactacaca agaattcatg gacgtggtgc ctggctgcag gtttgcagag ggaggccagg
     4741 agaaagaggt cgccttgccc tgtgtgctct gcagaggaat ccacgggtcc cacccaagac
     4801 cctccccaat tgtatgggga agtcgggagc cagtggctca ggtcttccat gctggacacc
     4861 taaggatctt ccccttcagc agcagcactg gccttgactg ctgctcttct gaaggcaggg
     4921 ctctgggcca tgtggaggcc tccagccttc taccctcccc aaccctggca ggctgatgcc
     4981 ctccccatgg ccactgagtg tgggacgcct gcccacctgc tactccactc caggacacca
     5041 agcactacct gtgcctaaga ctccagatca atcacacagt cgccacttct gctctttgtg
     5101 tgggttcctg gtgagaatag gacggggaca ggaggaagat ccaggggacc tgccacagca
     5161 aagaacagag acagtacaga ccccaagacc ggatgcctca ctcatttggg acggtcagaa
     5221 cgtgtctact tctctgcttg gatgggcttt aaattatccc ataggggcca atttcaaata
     5281 atttcttttc ctgatggaat ttaccttaat ctgtatataa cttgtaattt ttttttctaa
     5341 ttcatttctt tccttatttt atttcttcct taacagtatt tttggcattg gacaacttat
     5401 tctgaagaaa tgatgttaat ataagtatgt agtgaaggac caaaccgtgt gataaggttg
     5461 tgtgctgtgt gatcggtacc cagggagcgg ggcggtgtta atgtgccaaa tacccctgtg
     5521 tccctccctc caggagttcc acggtcacca ctgcccactt accagacaat catatgattt
     5581 tgttaaattt gagtttcagt tacattttaa gacaagtgtt ctatttattt tattgtttgg
     5641 aaggaggggg gcatatccca acaagataag aaggaacaga gaccgcctgg gttgtcattt
     5701 aaaactacaa gagattgtga ttgtgtgagc gtcagcaaac tcggagcacg cgtgtcatgc
     5761 cgtgggcctg tcagcggttc cctctgggag caacttaaac atccaagcac agatctttta
     5821 tatttgctaa ggccgttctg ggacaaagac cgtcatcttt gctgatgctc agtggtccag
     5881 gctacgctgg gatccgtgat ggggatatcc cagaagacac cattcccctg caggttgcgg
     5941 tgaatctcag tgacatagca tggaacagtc agtcctgtgc cgtgctcaga agcacactct
     6001 cgagtgcaca ctggacggcc tgaggcagca gggacactct gcagaggaac ttgccatggt
     6061 ccagtggtaa ggattcgtag gtctaactgc tcatgcttct catgggttcg tgacatcatc
     6121 tccatggcgg gccaggcctc ggcttgagaa tcccagtttc ctctaggtgg gagctagtcc
     6181 tgtgtaagtt caacctcaga tatacatgcc ccacttttgt aaaccccaaa ttatatgctt
     6241 tcttctcaga aagagcaagg tgtgtgcttt ttttttcccc cgcggtatga ctttctctct
     6301 ccggtacggg gagtctatct ggatggggat cttctgtgga cctttttgac agcagcagag
     6361 cagctaaagg ctcccttctc atcctctggc acgcacaacc cctgtccatc tctaacccaa
     6421 gagtcccatc tgtgctgagt taaatgcttt gcccccacaa agtgatgatt ccaaactcag
     6481 gttatcctga acagtcatcc ccttgggccc agcacttgga atttgaagga ctccgtcctt
     6541 gcttgccacc cccgacaaag acagcacttg attttctgca ctgagttcca gatattctgc
     6601 ctctactctg cattgccctc aaccagtgag ctctgcccac ctggcgtctc tgcagtctta
     6661 atagtgccca gctggctcaa gcaagcatca gatcaggtac aggagaccct ctcagagtca
     6721 ggaagctgga ccaggtgtga gttcaaaggt cttggccact ggagaaccag accattaact
     6781 tggctctctt gcctgttgct gtgcaggata gaggggcagg gcagaggttc agccacgatt
     6841 gtttcccaat gatactttcc attgtttgca cttgcctcct caaatagaaa ttcattcatc
     6901 gggaggccac ctagcttgca ttccctctag ccccgggcaa ggccacactt aggccctgtc
     6961 attgctgcca cacctaggat gatcaggtag gaaacgcagg gcttcactgt cctgcaggaa
     7021 ggtagatcct gcatgagtaa gaccccgtac atccctgtgc aaacccaggg tgtaaacctc
     7081 agtaatggga gagcatgaca tggccaagcc tgtggggccc tactctccaa ggccggggag
     7141 agcaggtaat ctacccagac ccagactgac ctgacccctc tgcctcccag ggtccagacc
     7201 cagagctctt ggcttcctct ccctcatctg cctccacagt gagtgtctgc gaagcacttg
     7261 cttaaataca tatcacgtgt tttagactcg aagctcaaag cactggattg tggtccctgt
     7321 ccctgcccaa gtggctgaga cctactgagc tgttcactgt gccgttcgag gtagggaaga
     7381 aaaggcctcc gagctgttgc tgggacagga gggtggagag gcctgggaca gacagcgctc
     7441 cctcctgcca gtagacactg ggacatccct gtccacggag atcagccggg tatcgaaagc
     7501 catgaagact gtccctagcc ctgagcgata ctgtgacaag acattcttga attctcccca
     7561 gacaaggtca cttcccccac cattccagcc attgtctgtc tgtcaccaat tcacatcctc
     7621 acttctagga gggggaaagg ttgagtcttt agatgtgcga ttgatcccct tgcaactggg
     7681 ggaagctggg ggtttcctca gtgtctccca cagcgcagct actctcttca gtgtggggaa
     7741 aggggggccc tggtgtcaga atacgtaact taaagcctgt gcgtgtggtg gtgcgtggtg
     7801 tgtgtgtgtg tgcatgtgtg ctgtatgtgt gtactttgaa ccaatagtat ataaatataa
     7861 atataaatat atatatatat ccctgacacc cgcgagaatc tccaccctgt atgagagttg
     7921 ccattgttac attcagatcg gattcagttc gtttctgttt ctgtttttgt tgttgttggg
     7981 tttttttttt tttcctttta gaactatata gtgttgagaa aggaatcaaa tgtaaatgtc
     8041 tggtttccat ataacgttaa aaaagaaaat taaaaaacca ttaaggaggg ggctggtgtc
     8101 tgccctgccc ccttccctgt aaattgctgt tgtgttgata aagctgtgca tggctcgtgt
     8161 ttagcggtca ttattgtgtc tgtttgtgga attcttatga tgaggaaaat cccgtagatg
     8221 cacttattga atatgtgatt aggtctaagt cagggccagg agtcccaaaa cgcccacaag
     8281 aaaaagataa aaagaaaaaa aaatggacca cagttcctgg tgactatgga aatcgccgtg
     8341 agagaatcgt gaatcgagaa gacgcggctt gagaatagtc aaatgtgatt tttttttttt
     8401 tttttttttt ttgcagatct cattctgagg cttatggcaa agacaaatgt acttgtgtag
     8461 aggtttctgt gaggtaaaag ccaggagggc ctggctttga ggtcaatgct gtagaatagg
     8521 actgactgga taccaaatgt aatctttccg atgctacaat gaatttatac acgagattga
     8581 tatgcaataa atctgtgtgt ttttc
//
