LOCUS       NM_001291029            8436 bp    mRNA    linear   ROD 31-OCT-2023
DEFINITION  Mus musculus PR domain containing 16 (Prdm16), transcript variant
            4, mRNA.
ACCESSION   NM_001291029 XM_006539177
VERSION     NM_001291029.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Hu T, Wu Q, Yao Q, Yu J, Jiang K, Wan Y and Tang Q.
  TITLE     PRDM16 exerts critical role in myocardial metabolism and energetics
            in type 2 diabetes induced cardiomyopathy
  JOURNAL   Metabolism 146, 155658 (2023)
   PUBMED   37433344
  REMARK    GeneRIF: PRDM16 exerts critical role in myocardial metabolism and
            energetics in type 2 diabetes induced cardiomyopathy.
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Kramer RJ, Fatahian AN, Chan A, Mortenson J, Osher J, Sun B, Parker
            LE, Rosamilia MB, Potter KB, Moore K, Atkins SL, Rosenfeld JA,
            Birjiniuk A, Jones E, Howard TS, Kim JJ, Scott DA, Lalani S,
            Rouzbehani OMT, Kaplan S, Hathaway MA, Cohen JL, Asaki SY, Martinez
            HR, Boudina S and Landstrom AP.
  TITLE     PRDM16 Deletion Is Associated With Sex-dependent Cardiomyopathy and
            Cardiac Mortality: A Translational, Multi-Institutional Cohort
            Study
  JOURNAL   Circ Genom Precis Med 16 (4), 390-400 (2023)
   PUBMED   37395136
  REMARK    GeneRIF: PRDM16 Deletion Is Associated With Sex-dependent
            Cardiomyopathy and Cardiac Mortality: A Translational,
            Multi-Institutional Cohort Study.
REFERENCE   3  (bases 1 to 8436)
  AUTHORS   Zhao S, Nie T, Li L, Long Q, Gu P, Zhang Y, Sun W, Lin Z, Liu Q, Qi
            Y, Wang W, Xie M, Loomes K, Cai C, Wu D and Hui HX.
  TITLE     Androgen Receptor is a Negative Regulator of PRDM16 in Beige
            Adipocyte
  JOURNAL   Adv Sci (Weinh) 10 (21), e2300070 (2023)
   PUBMED   37211698
  REMARK    GeneRIF: Androgen Receptor is a Negative Regulator of PRDM16 in
            Beige Adipocyte.
REFERENCE   4  (bases 1 to 8436)
  AUTHORS   Park A, Kim KE, Park I, Lee SH, Park KY, Jung M, Li X, Sleiman MB,
            Lee SJ, Kim DS, Kim J, Lim DS, Woo EJ, Lee EW, Han BS, Oh KJ, Lee
            SC, Auwerx J, Mun JY, Rhee HW, Kim WK, Bae KH and Suh JM.
  TITLE     Mitochondrial matrix protein LETMD1 maintains thermogenic capacity
            of brown adipose tissue in male mice
  JOURNAL   Nat Commun 14 (1), 3746 (2023)
   PUBMED   37353518
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8436)
  AUTHORS   Thompson M, Sakabe M, Verba M, Hao J, Meadows SM, Lu QR and Xin M.
  TITLE     PRDM16 regulates arterial development and vascular integrity
  JOURNAL   Front Physiol 14, 1165379 (2023)
   PUBMED   37324380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8436)
  AUTHORS   Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier
            G, Langin D and Spiegelman BM.
  TITLE     Transcriptional control of brown fat determination by PRDM16
  JOURNAL   Cell Metab 6 (1), 38-54 (2007)
   PUBMED   17618855
REFERENCE   7  (bases 1 to 8436)
  AUTHORS   Warner DR, Horn KH, Mudd L, Webb CL, Greene RM and Pisano MM.
  TITLE     PRDM16/MEL1: a novel Smad binding protein expressed in murine
            embryonic orofacial tissue
  JOURNAL   Biochim Biophys Acta 1773 (6), 814-820 (2007)
   PUBMED   17467076
  REMARK    GeneRIF: PRDM16/MEL1 is a Smad binding protein that may be
            important for development of orofacial structures through
            modulation of the TGFbeta signaling pathway.
REFERENCE   8  (bases 1 to 8436)
  AUTHORS   Du Y, Jenkins NA and Copeland NG.
  TITLE     Insertional mutagenesis identifies genes that promote the
            immortalization of primary bone marrow progenitor cells
  JOURNAL   Blood 106 (12), 3932-3939 (2005)
   PUBMED   16109773
REFERENCE   9  (bases 1 to 8436)
  AUTHORS   Nishikata I, Sasaki H, Iga M, Tateno Y, Imayoshi S, Asou N,
            Nakamura T and Morishita K.
  TITLE     A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
            expressed mainly in t(1;3)(p36;q21)-positive AML and blocks
            G-CSF-induced myeloid differentiation
  JOURNAL   Blood 102 (9), 3323-3332 (2003)
   PUBMED   12816872
REFERENCE   10 (bases 1 to 8436)
  AUTHORS   Herron BJ, Lu W, Rao C, Liu S, Peters H, Bronson RT, Justice MJ,
            McDonald JD and Beier DR.
  TITLE     Efficient generation and mapping of recessive developmental
            mutations using ENU mutagenesis
  JOURNAL   Nat Genet 30 (2), 185-189 (2002)
   PUBMED   11818962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY739066.1, BC059838.1 and AL627123.13.
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_001291029.2.
            
            On Mar 22, 2014 this sequence version replaced XM_006539177.1.
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice site each in the 5' and 3' coding regions, and lacks an
            alternate exon in the 3' coding region, compared to variant 1. It
            encodes isoform 4, which is shorter and has a distinct C-terminus,
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059838.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY739066.1         4-25
            23-4285             BC059838.1         1-4263
            4286-8436           AL627123.13        149763-153913       c
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.96 cM"
     gene            1..8436
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /note="PR domain containing 16"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
     exon            1..151
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3651
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /EC_number="2.1.1.367"
                     /note="isoform 4 is encoded by transcript variant 4;
                     transcription factor MEL1; MDS1/EVI1-like gene 1; PR
                     domain-containing protein 16; PR domain zinc finger
                     protein 16; line 27; histone-lysine N-methyltransferase
                     PRDM16"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase PRDM16
                     isoform 4"
                     /protein_id="NP_001277958.1"
                     /db_xref="CCDS:CCDS71532.1"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
                     /translation="MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILS
                     PIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKR
                     KMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKQISEDLGSEKFCVD
                     ANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSL
                     GVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGV
                     GSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHD
                     SGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHST
                     VKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGK
                     NHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPA
                     FPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSN
                     SSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTG
                     SDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEE
                     QLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLY
                     PFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPS
                     SGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLH
                     YAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFA
                     AMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHL
                     RTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLK
                     KHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMN
                     QASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVS
                     PTPEPQGVYEDEEDEEPPSLTMGFDHTRRHMQ"
     exon            152..501
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..552
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            553..690
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            691..793
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            794..1004
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1152
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1306
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..2723
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2811
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2981
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..3059
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..3229
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3404
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..3638
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..8436
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgctctgtg cgcatgtgcg aaggtgtcca aactgacaat gctagggaga tgaagatagt
       61 gtgtagctgc ttctgggctc aaggaggagg agagagattc cgcgagccga caccatgcga
      121 tccaaggcga gggcgaggaa gctagccaaa agtgacggtg acgttgtaaa taatatgtat
      181 gaacctgacc cggacctgct ggccggccag agtgccgagg aggagaccga agacggcatc
      241 ctgtccccca tccccatggg gccaccgtcc cccttcccca ccagcgagga cttcactccc
      301 aaggagggct cgccctatga ggctcctgtc tacattcctg aagacattcc aatcccacca
      361 gacttcgagc tacgagagtc ctccatacca ggagctggcc tggggatctg ggccaagcgg
      421 aagatggaaa tcggggagag gtttggcccc tacgtggtga cgccccgggc cgcactgaag
      481 gaggccgact ttggatggga gcagatgctg acggatacag aggtgtcatc ccaggagagc
      541 tgcatcaaaa agcagatctc tgaagacttg ggtagcgaga agttctgcgt ggatgccaat
      601 caggcggggt ctggcagctg gctcaagtac atccgtgtag cgtgttcctg tgatgaccaa
      661 aacctcgcca tgtgtcagat caacgaacag atttactata aagtcattaa ggacatcgag
      721 cctggagagg aactgttggt gcatgtgaaa gaaggtgcct actccttggg tgtcatggcc
      781 cccagcttgg atgaggaccc cacattccgc tgtgatgagt gtgatgagct cttccagtgc
      841 aggctggacc tgaggcgcca caagaagtac gcgtgcagct ctgcaggagc ccagctctac
      901 gagggcctag gggaggaact caagcccgag ggccttggcg tgggcagcga cgggcaagcg
      961 catgagtgca aggattgcga gcggatgttc cccaacaagt acagcttgga gcaacacatg
     1021 atcgtccaca cggaagagcg tgagtacaaa tgtgaccagt gtcccaaggc cttcaactgg
     1081 aagtccaacc tcatccgcca ccagatgtct cacgacagtg gcaagcgctt cgaatgtgaa
     1141 aactgtgtca aggtgttcac ggaccccagc aacctccagc gtcacatccg ctcacagcat
     1201 gtcggtgccc gggcccatgc ctgccctgac tgtggcaaga ccttcgccac atcctctggc
     1261 ctcaaacagc acaagcatat ccacagcacg gtgaagccat tcatatgcga ggtctgccac
     1321 aagtcctaca cgcagttctc caacctgtgc cggcacaagc ggatgcacgc cgactgcagg
     1381 acgcagatca agtgcaagga ctgtgggcag atgttcagca ctacctcctc cctcaacaag
     1441 catcggagat tctgcgaggg caagaaccat tacacgcctg gcagcatctt caccccaggc
     1501 ctgcccttga cccccagccc catgatggac aagacaaaac cctccccgac cctcaaccac
     1561 gggggcctag gcttcagcga gtacttcccc tccagacctc atcctgggag cctgcccttc
     1621 tcggctgctc ctccggcctt ccccgcactc actccgggct tcccgggcat ctttcctcca
     1681 tccctgtacc cacgaccacc tctgctacct cccacgccgc tgctcaagag ccccctgaac
     1741 cacgcgcagg acgccaagct acccagcccg ctgggaaacc cagccctgcc ccttgtctcc
     1801 gcggtcagca atagcagcca gggtgccaca gcggccaccg ggtcagagga gaaatttgat
     1861 ggccgcttgg aagacgcata tgcggagaag gtcaaaaata ggagccctga catgtcggat
     1921 ggcagtgact ttgaggatat caacaccacg accgggacag acttggacac taccacgggc
     1981 acggggtcag acctggacag cgacctggac agtgacagag acaaaggcaa ggacaagggg
     2041 aagccagtgg agagcaaacc tgagtttggg ggtgcatctg tgccccctgg ggccatgaac
     2101 agtgtggccg aggtaccggc cttctactca cagcattcct tcttcccgcc acccgaggaa
     2161 cagctgctga cggcctcggg agctgccggc gactccatca aggccatcgc gtccatcgcg
     2221 gagaaatact tcggtcctgg cttcatgagc atgcaggaga agaagctggg ctcactaccc
     2281 taccactccg tgttcccctt ccagttcctg cctaactttc cccactccct ctaccccttt
     2341 acggaccgag ccctcgccca caacttgctg gtcaaggctg agccaaagtc accccgggat
     2401 gccctcaagg tgggcggccc cagtgcggag tgccccttcg acctcaccac caaaccaaaa
     2461 gaggccaaac ccgccctgct cgcacccaag gtccccctca tcccctcatc tggcgaggaa
     2521 cagccactgg acctgagcat cggcagcagg gccagggcaa gccagaacgg aggtggccgt
     2581 gagccgcgga agaaccacgt ctacggtgaa cggaagccgg gggtcagcga ggggctgcct
     2641 aaggtgtgcc cagcacagct gccccagcag ccctccttgc attatgctaa gccttcaccg
     2701 ttcttcatgg atcccatcta cagcagggta gaaaagcgga aggtggcaga ccctgtggga
     2761 gtcctgaaag agaagtacct gcggccgtcc ccacttctgt tccaccccca gatgtcagcc
     2821 atagaaacca tgacggagaa gctggagagc tttgcagcca tgaaggccga ctcaggcagc
     2881 tccctgcagc ccctgcctca ccacccgttc aacttccgct ccccaccccc aacgctctcg
     2941 gatcccatcc tcaggaaggg gaaggagaga tacacgtgca ggtactgtgg caagatcttc
     3001 cccagatctg caaatctcac aagacatctg aggacacaca caggggagca gccatacagg
     3061 tgcaagtact gtgaccggtc attcagcatc tcctccaacc tccagcggca cgtgaggaac
     3121 atccacaaca aagagaagcc gttcaagtgc catctgtgca accgctgctt cgggcagcag
     3181 accaacctag accggcacct gaagaagcac gaacacgagg gcgcaccagt gagccagcac
     3241 tccggggtgc tcacgaacca cctgggcacc agcgcctcct cccccacctc cgagtcggac
     3301 aaccatgcac ttttagatga gaaggaagat tcttacttct ccgagatccg aaacttcatc
     3361 gccaacagcg agatgaacca ggcatccact cgaatggaca aacggcctga gatccaagac
     3421 ctggacagca acccaccgtg tccaggctca gccagtgcaa agccagagga cgtagaggag
     3481 gaggaagagg aggagctgga ggaagaggat gatgacagct tagccgggaa gtcacaggag
     3541 gacacggtgt cccccacacc tgagccccaa ggagtctatg aagatgaaga ggatgaggaa
     3601 ccacccagcc tgaccatggg ctttgaccat acccggaggc atatgcaatg atgctgtccc
     3661 tctctgaaga cactcctctc cacgccccct cccagagctc actggatgct tggttgaaca
     3721 tcacaggacc ctcgtcagag tccggagcct ttaaccccat caaccacctc tgaggtcctg
     3781 gaggccccag ggccagagtc caaagccagg gtaccagctg caggagatgg agaaggggcc
     3841 agggagcagc cccccaccct caacacctcc actttgcaaa gtccagcttc tccattgaaa
     3901 ctcagaaccc gaaggtccct tgagtagccg ctggccttca tcacctctca gaactggcct
     3961 caaggacacg atctgcagtg ggtgcggtgc acgggccacc caggagctgc tcacaggagc
     4021 catggatcag aaaactcgtg ggcaagggtg gggtctctat cccagcagga gccagttggc
     4081 cacatccagg caactgcatg gtatgaaaga ggaaatcgga aagacgtggg caagtgctat
     4141 ggagagagac ctcatcaatg atttttataa tgagaatcac atgattaagc cttttggtaa
     4201 tcttattgac tatagagtct atttaagcat gtgggtttta aaaaaaatag acggtatttt
     4261 ttaaaaatca aaaaaaaaaa aaaaagactt gcaaaatgtt tttttaaaag taattttgca
     4321 tcgctttgga atttcagctt attggcaaac ccacacctgc caggacatcg agtacggctc
     4381 gggtgtgccc tttataatgt aaataatgga gaattttttt tctatctctg acctgtttgt
     4441 ataagccaag gtgattccag tgtgcccgac acaggataag gggcatggag ccactctgct
     4501 aagtcagatg ctgactacac aagaattcat ggacgtggtg cctggctgca ggtttgcaga
     4561 gggaggccag gagaaagagg tcgccttgcc ctgtgtgctc tgcagaggaa tccacgggtc
     4621 ccacccaaga ccctccccaa ttgtatgggg aagtcgggag ccagtggctc aggtcttcca
     4681 tgctggacac ctaaggatct tccccttcag cagcagcact ggccttgact gctgctcttc
     4741 tgaaggcagg gctctgggcc atgtggaggc ctccagcctt ctaccctccc caaccctggc
     4801 aggctgatgc cctccccatg gccactgagt gtgggacgcc tgcccacctg ctactccact
     4861 ccaggacacc aagcactacc tgtgcctaag actccagatc aatcacacag tcgccacttc
     4921 tgctctttgt gtgggttcct ggtgagaata ggacggggac aggaggaaga tccaggggac
     4981 ctgccacagc aaagaacaga gacagtacag accccaagac cggatgcctc actcatttgg
     5041 gacggtcaga acgtgtctac ttctctgctt ggatgggctt taaattatcc cataggggcc
     5101 aatttcaaat aatttctttt cctgatggaa tttaccttaa tctgtatata acttgtaatt
     5161 tttttttcta attcatttct ttccttattt tatttcttcc ttaacagtat ttttggcatt
     5221 ggacaactta ttctgaagaa atgatgttaa tataagtatg tagtgaagga ccaaaccgtg
     5281 tgataaggtt gtgtgctgtg tgatcggtac ccagggagcg gggcggtgtt aatgtgccaa
     5341 atacccctgt gtccctccct ccaggagttc cacggtcacc actgcccact taccagacaa
     5401 tcatatgatt ttgttaaatt tgagtttcag ttacatttta agacaagtgt tctatttatt
     5461 ttattgtttg gaaggagggg ggcatatccc aacaagataa gaaggaacag agaccgcctg
     5521 ggttgtcatt taaaactaca agagattgtg attgtgtgag cgtcagcaaa ctcggagcac
     5581 gcgtgtcatg ccgtgggcct gtcagcggtt ccctctggga gcaacttaaa catccaagca
     5641 cagatctttt atatttgcta aggccgttct gggacaaaga ccgtcatctt tgctgatgct
     5701 cagtggtcca ggctacgctg ggatccgtga tggggatatc ccagaagaca ccattcccct
     5761 gcaggttgcg gtgaatctca gtgacatagc atggaacagt cagtcctgtg ccgtgctcag
     5821 aagcacactc tcgagtgcac actggacggc ctgaggcagc agggacactc tgcagaggaa
     5881 cttgccatgg tccagtggta aggattcgta ggtctaactg ctcatgcttc tcatgggttc
     5941 gtgacatcat ctccatggcg ggccaggcct cggcttgaga atcccagttt cctctaggtg
     6001 ggagctagtc ctgtgtaagt tcaacctcag atatacatgc cccacttttg taaaccccaa
     6061 attatatgct ttcttctcag aaagagcaag gtgtgtgctt tttttttccc ccgcggtatg
     6121 actttctctc tccggtacgg ggagtctatc tggatgggga tcttctgtgg acctttttga
     6181 cagcagcaga gcagctaaag gctcccttct catcctctgg cacgcacaac ccctgtccat
     6241 ctctaaccca agagtcccat ctgtgctgag ttaaatgctt tgcccccaca aagtgatgat
     6301 tccaaactca ggttatcctg aacagtcatc cccttgggcc cagcacttgg aatttgaagg
     6361 actccgtcct tgcttgccac ccccgacaaa gacagcactt gattttctgc actgagttcc
     6421 agatattctg cctctactct gcattgccct caaccagtga gctctgccca cctggcgtct
     6481 ctgcagtctt aatagtgccc agctggctca agcaagcatc agatcaggta caggagaccc
     6541 tctcagagtc aggaagctgg accaggtgtg agttcaaagg tcttggccac tggagaacca
     6601 gaccattaac ttggctctct tgcctgttgc tgtgcaggat agaggggcag ggcagaggtt
     6661 cagccacgat tgtttcccaa tgatactttc cattgtttgc acttgcctcc tcaaatagaa
     6721 attcattcat cgggaggcca cctagcttgc attccctcta gccccgggca aggccacact
     6781 taggccctgt cattgctgcc acacctagga tgatcaggta ggaaacgcag ggcttcactg
     6841 tcctgcagga aggtagatcc tgcatgagta agaccccgta catccctgtg caaacccagg
     6901 gtgtaaacct cagtaatggg agagcatgac atggccaagc ctgtggggcc ctactctcca
     6961 aggccgggga gagcaggtaa tctacccaga cccagactga cctgacccct ctgcctccca
     7021 gggtccagac ccagagctct tggcttcctc tccctcatct gcctccacag tgagtgtctg
     7081 cgaagcactt gcttaaatac atatcacgtg ttttagactc gaagctcaaa gcactggatt
     7141 gtggtccctg tccctgccca agtggctgag acctactgag ctgttcactg tgccgttcga
     7201 ggtagggaag aaaaggcctc cgagctgttg ctgggacagg agggtggaga ggcctgggac
     7261 agacagcgct ccctcctgcc agtagacact gggacatccc tgtccacgga gatcagccgg
     7321 gtatcgaaag ccatgaagac tgtccctagc cctgagcgat actgtgacaa gacattcttg
     7381 aattctcccc agacaaggtc acttccccca ccattccagc cattgtctgt ctgtcaccaa
     7441 ttcacatcct cacttctagg agggggaaag gttgagtctt tagatgtgcg attgatcccc
     7501 ttgcaactgg gggaagctgg gggtttcctc agtgtctccc acagcgcagc tactctcttc
     7561 agtgtgggga aaggggggcc ctggtgtcag aatacgtaac ttaaagcctg tgcgtgtggt
     7621 ggtgcgtggt gtgtgtgtgt gtgcatgtgt gctgtatgtg tgtactttga accaatagta
     7681 tataaatata aatataaata tatatatata tccctgacac ccgcgagaat ctccaccctg
     7741 tatgagagtt gccattgtta cattcagatc ggattcagtt cgtttctgtt tctgtttttg
     7801 ttgttgttgg gttttttttt ttttcctttt agaactatat agtgttgaga aaggaatcaa
     7861 atgtaaatgt ctggtttcca tataacgtta aaaaagaaaa ttaaaaaacc attaaggagg
     7921 gggctggtgt ctgccctgcc cccttccctg taaattgctg ttgtgttgat aaagctgtgc
     7981 atggctcgtg tttagcggtc attattgtgt ctgtttgtgg aattcttatg atgaggaaaa
     8041 tcccgtagat gcacttattg aatatgtgat taggtctaag tcagggccag gagtcccaaa
     8101 acgcccacaa gaaaaagata aaaagaaaaa aaaatggacc acagttcctg gtgactatgg
     8161 aaatcgccgt gagagaatcg tgaatcgaga agacgcggct tgagaatagt caaatgtgat
     8221 tttttttttt tttttttttt tttgcagatc tcattctgag gcttatggca aagacaaatg
     8281 tacttgtgta gaggtttctg tgaggtaaaa gccaggaggg cctggctttg aggtcaatgc
     8341 tgtagaatag gactgactgg ataccaaatg taatctttcc gatgctacaa tgaatttata
     8401 cacgagattg atatgcaata aatctgtgtg tttttc
//
