LOCUS       NM_001291026            8602 bp    mRNA    linear   ROD 31-OCT-2023
DEFINITION  Mus musculus PR domain containing 16 (Prdm16), transcript variant
            3, mRNA.
ACCESSION   NM_001291026 XM_006539176
VERSION     NM_001291026.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Hu T, Wu Q, Yao Q, Yu J, Jiang K, Wan Y and Tang Q.
  TITLE     PRDM16 exerts critical role in myocardial metabolism and energetics
            in type 2 diabetes induced cardiomyopathy
  JOURNAL   Metabolism 146, 155658 (2023)
   PUBMED   37433344
  REMARK    GeneRIF: PRDM16 exerts critical role in myocardial metabolism and
            energetics in type 2 diabetes induced cardiomyopathy.
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Kramer RJ, Fatahian AN, Chan A, Mortenson J, Osher J, Sun B, Parker
            LE, Rosamilia MB, Potter KB, Moore K, Atkins SL, Rosenfeld JA,
            Birjiniuk A, Jones E, Howard TS, Kim JJ, Scott DA, Lalani S,
            Rouzbehani OMT, Kaplan S, Hathaway MA, Cohen JL, Asaki SY, Martinez
            HR, Boudina S and Landstrom AP.
  TITLE     PRDM16 Deletion Is Associated With Sex-dependent Cardiomyopathy and
            Cardiac Mortality: A Translational, Multi-Institutional Cohort
            Study
  JOURNAL   Circ Genom Precis Med 16 (4), 390-400 (2023)
   PUBMED   37395136
  REMARK    GeneRIF: PRDM16 Deletion Is Associated With Sex-dependent
            Cardiomyopathy and Cardiac Mortality: A Translational,
            Multi-Institutional Cohort Study.
REFERENCE   3  (bases 1 to 8602)
  AUTHORS   Zhao S, Nie T, Li L, Long Q, Gu P, Zhang Y, Sun W, Lin Z, Liu Q, Qi
            Y, Wang W, Xie M, Loomes K, Cai C, Wu D and Hui HX.
  TITLE     Androgen Receptor is a Negative Regulator of PRDM16 in Beige
            Adipocyte
  JOURNAL   Adv Sci (Weinh) 10 (21), e2300070 (2023)
   PUBMED   37211698
  REMARK    GeneRIF: Androgen Receptor is a Negative Regulator of PRDM16 in
            Beige Adipocyte.
REFERENCE   4  (bases 1 to 8602)
  AUTHORS   Park A, Kim KE, Park I, Lee SH, Park KY, Jung M, Li X, Sleiman MB,
            Lee SJ, Kim DS, Kim J, Lim DS, Woo EJ, Lee EW, Han BS, Oh KJ, Lee
            SC, Auwerx J, Mun JY, Rhee HW, Kim WK, Bae KH and Suh JM.
  TITLE     Mitochondrial matrix protein LETMD1 maintains thermogenic capacity
            of brown adipose tissue in male mice
  JOURNAL   Nat Commun 14 (1), 3746 (2023)
   PUBMED   37353518
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8602)
  AUTHORS   Thompson M, Sakabe M, Verba M, Hao J, Meadows SM, Lu QR and Xin M.
  TITLE     PRDM16 regulates arterial development and vascular integrity
  JOURNAL   Front Physiol 14, 1165379 (2023)
   PUBMED   37324380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8602)
  AUTHORS   Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier
            G, Langin D and Spiegelman BM.
  TITLE     Transcriptional control of brown fat determination by PRDM16
  JOURNAL   Cell Metab 6 (1), 38-54 (2007)
   PUBMED   17618855
REFERENCE   7  (bases 1 to 8602)
  AUTHORS   Warner DR, Horn KH, Mudd L, Webb CL, Greene RM and Pisano MM.
  TITLE     PRDM16/MEL1: a novel Smad binding protein expressed in murine
            embryonic orofacial tissue
  JOURNAL   Biochim Biophys Acta 1773 (6), 814-820 (2007)
   PUBMED   17467076
  REMARK    GeneRIF: PRDM16/MEL1 is a Smad binding protein that may be
            important for development of orofacial structures through
            modulation of the TGFbeta signaling pathway.
REFERENCE   8  (bases 1 to 8602)
  AUTHORS   Du Y, Jenkins NA and Copeland NG.
  TITLE     Insertional mutagenesis identifies genes that promote the
            immortalization of primary bone marrow progenitor cells
  JOURNAL   Blood 106 (12), 3932-3939 (2005)
   PUBMED   16109773
REFERENCE   9  (bases 1 to 8602)
  AUTHORS   Nishikata I, Sasaki H, Iga M, Tateno Y, Imayoshi S, Asou N,
            Nakamura T and Morishita K.
  TITLE     A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
            expressed mainly in t(1;3)(p36;q21)-positive AML and blocks
            G-CSF-induced myeloid differentiation
  JOURNAL   Blood 102 (9), 3323-3332 (2003)
   PUBMED   12816872
REFERENCE   10 (bases 1 to 8602)
  AUTHORS   Herron BJ, Lu W, Rao C, Liu S, Peters H, Bronson RT, Justice MJ,
            McDonald JD and Beier DR.
  TITLE     Efficient generation and mapping of recessive developmental
            mutations using ENU mutagenesis
  JOURNAL   Nat Genet 30 (2), 185-189 (2002)
   PUBMED   11818962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY739066.1, BC059838.1, AK083829.1 and AL627123.13.
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_001291026.2.
            
            On Mar 22, 2014 this sequence version replaced XM_006539176.1.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. It
            encodes isoform 3, which is shorter by an amino acid, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083829.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY739066.1         4-25
            23-501              BC059838.1         1-479
            502-549             BC059838.1         483-530
            550-2717            BC059838.1         534-2701
            2718-3629           BC059838.1         2705-3616
            3630-3804           AK083829.1         3521-3695
            3805-4451           BC059838.1         3617-4263
            4452-8602           AL627123.13        149763-153913       c
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.96 cM"
     gene            1..8602
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /note="PR domain containing 16"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
     exon            1..151
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3939
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /EC_number="2.1.1.367"
                     /note="isoform 3 is encoded by transcript variant 3;
                     transcription factor MEL1; MDS1/EVI1-like gene 1; PR
                     domain-containing protein 16; PR domain zinc finger
                     protein 16; line 27; histone-lysine N-methyltransferase
                     PRDM16"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase PRDM16
                     isoform 3"
                     /protein_id="NP_001277955.1"
                     /db_xref="CCDS:CCDS71530.1"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
                     /translation="MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILS
                     PIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKR
                     KMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKISEDLGSEKFCVDAN
                     QAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGV
                     MAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGS
                     DGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSG
                     KRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVK
                     PFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNH
                     YTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFP
                     ALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSS
                     QGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSD
                     LDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQL
                     LTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPF
                     TDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSG
                     EEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYA
                     KPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMK
                     ADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTH
                     TGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHE
                     HEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQAS
                     TRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTP
                     EPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAF
                     EVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPS
                     SESGAFNPINHL"
     exon            152..501
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..549
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            550..684
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            685..787
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            788..998
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1146
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1300
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..2717
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2802
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2972
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3050
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3220
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3395
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3396..3629
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3804
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..8602
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgctctgtg cgcatgtgcg aaggtgtcca aactgacaat gctagggaga tgaagatagt
       61 gtgtagctgc ttctgggctc aaggaggagg agagagattc cgcgagccga caccatgcga
      121 tccaaggcga gggcgaggaa gctagccaaa agtgacggtg acgttgtaaa taatatgtat
      181 gaacctgacc cggacctgct ggccggccag agtgccgagg aggagaccga agacggcatc
      241 ctgtccccca tccccatggg gccaccgtcc cccttcccca ccagcgagga cttcactccc
      301 aaggagggct cgccctatga ggctcctgtc tacattcctg aagacattcc aatcccacca
      361 gacttcgagc tacgagagtc ctccatacca ggagctggcc tggggatctg ggccaagcgg
      421 aagatggaaa tcggggagag gtttggcccc tacgtggtga cgccccgggc cgcactgaag
      481 gaggccgact ttggatggga gatgctgacg gatacagagg tgtcatccca ggagagctgc
      541 atcaaaaaga tctctgaaga cttgggtagc gagaagttct gcgtggatgc caatcaggcg
      601 gggtctggca gctggctcaa gtacatccgt gtagcgtgtt cctgtgatga ccaaaacctc
      661 gccatgtgtc agatcaacga acagatttac tataaagtca ttaaggacat cgagcctgga
      721 gaggaactgt tggtgcatgt gaaagaaggt gcctactcct tgggtgtcat ggcccccagc
      781 ttggatgagg accccacatt ccgctgtgat gagtgtgatg agctcttcca gtgcaggctg
      841 gacctgaggc gccacaagaa gtacgcgtgc agctctgcag gagcccagct ctacgagggc
      901 ctaggggagg aactcaagcc cgagggcctt ggcgtgggca gcgacgggca agcgcatgag
      961 tgcaaggatt gcgagcggat gttccccaac aagtacagct tggagcaaca catgatcgtc
     1021 cacacggaag agcgtgagta caaatgtgac cagtgtccca aggccttcaa ctggaagtcc
     1081 aacctcatcc gccaccagat gtctcacgac agtggcaagc gcttcgaatg tgaaaactgt
     1141 gtcaaggtgt tcacggaccc cagcaacctc cagcgtcaca tccgctcaca gcatgtcggt
     1201 gcccgggccc atgcctgccc tgactgtggc aagaccttcg ccacatcctc tggcctcaaa
     1261 cagcacaagc atatccacag cacggtgaag ccattcatat gcgaggtctg ccacaagtcc
     1321 tacacgcagt tctccaacct gtgccggcac aagcggatgc acgccgactg caggacgcag
     1381 atcaagtgca aggactgtgg gcagatgttc agcactacct cctccctcaa caagcatcgg
     1441 agattctgcg agggcaagaa ccattacacg cctggcagca tcttcacccc aggcctgccc
     1501 ttgaccccca gccccatgat ggacaagaca aaaccctccc cgaccctcaa ccacgggggc
     1561 ctaggcttca gcgagtactt cccctccaga cctcatcctg ggagcctgcc cttctcggct
     1621 gctcctccgg ccttccccgc actcactccg ggcttcccgg gcatctttcc tccatccctg
     1681 tacccacgac cacctctgct acctcccacg ccgctgctca agagccccct gaaccacgcg
     1741 caggacgcca agctacccag cccgctggga aacccagccc tgccccttgt ctccgcggtc
     1801 agcaatagca gccagggtgc cacagcggcc accgggtcag aggagaaatt tgatggccgc
     1861 ttggaagacg catatgcgga gaaggtcaaa aataggagcc ctgacatgtc ggatggcagt
     1921 gactttgagg atatcaacac cacgaccggg acagacttgg acactaccac gggcacgggg
     1981 tcagacctgg acagcgacct ggacagtgac agagacaaag gcaaggacaa ggggaagcca
     2041 gtggagagca aacctgagtt tgggggtgca tctgtgcccc ctggggccat gaacagtgtg
     2101 gccgaggtac cggccttcta ctcacagcat tccttcttcc cgccacccga ggaacagctg
     2161 ctgacggcct cgggagctgc cggcgactcc atcaaggcca tcgcgtccat cgcggagaaa
     2221 tacttcggtc ctggcttcat gagcatgcag gagaagaagc tgggctcact accctaccac
     2281 tccgtgttcc ccttccagtt cctgcctaac tttccccact ccctctaccc ctttacggac
     2341 cgagccctcg cccacaactt gctggtcaag gctgagccaa agtcaccccg ggatgccctc
     2401 aaggtgggcg gccccagtgc ggagtgcccc ttcgacctca ccaccaaacc aaaagaggcc
     2461 aaacccgccc tgctcgcacc caaggtcccc ctcatcccct catctggcga ggaacagcca
     2521 ctggacctga gcatcggcag cagggccagg gcaagccaga acggaggtgg ccgtgagccg
     2581 cggaagaacc acgtctacgg tgaacggaag ccgggggtca gcgaggggct gcctaaggtg
     2641 tgcccagcac agctgcccca gcagccctcc ttgcattatg ctaagccttc accgttcttc
     2701 atggatccca tctacagggt agaaaagcgg aaggtggcag accctgtggg agtcctgaaa
     2761 gagaagtacc tgcggccgtc cccacttctg ttccaccccc agatgtcagc catagaaacc
     2821 atgacggaga agctggagag ctttgcagcc atgaaggccg actcaggcag ctccctgcag
     2881 cccctgcctc accacccgtt caacttccgc tccccacccc caacgctctc ggatcccatc
     2941 ctcaggaagg ggaaggagag atacacgtgc aggtactgtg gcaagatctt ccccagatct
     3001 gcaaatctca caagacatct gaggacacac acaggggagc agccatacag gtgcaagtac
     3061 tgtgaccggt cattcagcat ctcctccaac ctccagcggc acgtgaggaa catccacaac
     3121 aaagagaagc cgttcaagtg ccatctgtgc aaccgctgct tcgggcagca gaccaaccta
     3181 gaccggcacc tgaagaagca cgaacacgag ggcgcaccag tgagccagca ctccggggtg
     3241 ctcacgaacc acctgggcac cagcgcctcc tcccccacct ccgagtcgga caaccatgca
     3301 cttttagatg agaaggaaga ttcttacttc tccgagatcc gaaacttcat cgccaacagc
     3361 gagatgaacc aggcatccac tcgaatggac aaacggcctg agatccaaga cctggacagc
     3421 aacccaccgt gtccaggctc agccagtgca aagccagagg acgtagagga ggaggaagag
     3481 gaggagctgg aggaagagga tgatgacagc ttagccggga agtcacagga ggacacggtg
     3541 tcccccacac ctgagcccca aggagtctat gaagatgaag aggatgagga accacccagc
     3601 ctgaccatgg gctttgacca tacccggagg tgtgttgagg agcgaggagg cggcctgtta
     3661 gctttggagc cgacgccgac ctttgggaag gggctggatc tccgcagagc agctgaggaa
     3721 gcatttgaag ttaaagatgt gcttaattcc accttagatt ctgaggtttt aaaacaaacc
     3781 ctgtacaggc aggctaagaa ccaggcatat gcaatgatgc tgtccctctc tgaagacact
     3841 cctctccacg ccccctccca gagctcactg gatgcttggt tgaacatcac aggaccctcg
     3901 tcagagtccg gagcctttaa ccccatcaac cacctctgag gtcctggagg ccccagggcc
     3961 agagtccaaa gccagggtac cagctgcagg agatggagaa ggggccaggg agcagccccc
     4021 caccctcaac acctccactt tgcaaagtcc agcttctcca ttgaaactca gaacccgaag
     4081 gtcccttgag tagccgctgg ccttcatcac ctctcagaac tggcctcaag gacacgatct
     4141 gcagtgggtg cggtgcacgg gccacccagg agctgctcac aggagccatg gatcagaaaa
     4201 ctcgtgggca agggtggggt ctctatccca gcaggagcca gttggccaca tccaggcaac
     4261 tgcatggtat gaaagaggaa atcggaaaga cgtgggcaag tgctatggag agagacctca
     4321 tcaatgattt ttataatgag aatcacatga ttaagccttt tggtaatctt attgactata
     4381 gagtctattt aagcatgtgg gttttaaaaa aaatagacgg tattttttaa aaatcaaaaa
     4441 aaaaaaaaaa agacttgcaa aatgtttttt taaaagtaat tttgcatcgc tttggaattt
     4501 cagcttattg gcaaacccac acctgccagg acatcgagta cggctcgggt gtgcccttta
     4561 taatgtaaat aatggagaat tttttttcta tctctgacct gtttgtataa gccaaggtga
     4621 ttccagtgtg cccgacacag gataaggggc atggagccac tctgctaagt cagatgctga
     4681 ctacacaaga attcatggac gtggtgcctg gctgcaggtt tgcagaggga ggccaggaga
     4741 aagaggtcgc cttgccctgt gtgctctgca gaggaatcca cgggtcccac ccaagaccct
     4801 ccccaattgt atggggaagt cgggagccag tggctcaggt cttccatgct ggacacctaa
     4861 ggatcttccc cttcagcagc agcactggcc ttgactgctg ctcttctgaa ggcagggctc
     4921 tgggccatgt ggaggcctcc agccttctac cctccccaac cctggcaggc tgatgccctc
     4981 cccatggcca ctgagtgtgg gacgcctgcc cacctgctac tccactccag gacaccaagc
     5041 actacctgtg cctaagactc cagatcaatc acacagtcgc cacttctgct ctttgtgtgg
     5101 gttcctggtg agaataggac ggggacagga ggaagatcca ggggacctgc cacagcaaag
     5161 aacagagaca gtacagaccc caagaccgga tgcctcactc atttgggacg gtcagaacgt
     5221 gtctacttct ctgcttggat gggctttaaa ttatcccata ggggccaatt tcaaataatt
     5281 tcttttcctg atggaattta ccttaatctg tatataactt gtaatttttt tttctaattc
     5341 atttctttcc ttattttatt tcttccttaa cagtattttt ggcattggac aacttattct
     5401 gaagaaatga tgttaatata agtatgtagt gaaggaccaa accgtgtgat aaggttgtgt
     5461 gctgtgtgat cggtacccag ggagcggggc ggtgttaatg tgccaaatac ccctgtgtcc
     5521 ctccctccag gagttccacg gtcaccactg cccacttacc agacaatcat atgattttgt
     5581 taaatttgag tttcagttac attttaagac aagtgttcta tttattttat tgtttggaag
     5641 gaggggggca tatcccaaca agataagaag gaacagagac cgcctgggtt gtcatttaaa
     5701 actacaagag attgtgattg tgtgagcgtc agcaaactcg gagcacgcgt gtcatgccgt
     5761 gggcctgtca gcggttccct ctgggagcaa cttaaacatc caagcacaga tcttttatat
     5821 ttgctaaggc cgttctggga caaagaccgt catctttgct gatgctcagt ggtccaggct
     5881 acgctgggat ccgtgatggg gatatcccag aagacaccat tcccctgcag gttgcggtga
     5941 atctcagtga catagcatgg aacagtcagt cctgtgccgt gctcagaagc acactctcga
     6001 gtgcacactg gacggcctga ggcagcaggg acactctgca gaggaacttg ccatggtcca
     6061 gtggtaagga ttcgtaggtc taactgctca tgcttctcat gggttcgtga catcatctcc
     6121 atggcgggcc aggcctcggc ttgagaatcc cagtttcctc taggtgggag ctagtcctgt
     6181 gtaagttcaa cctcagatat acatgcccca cttttgtaaa ccccaaatta tatgctttct
     6241 tctcagaaag agcaaggtgt gtgctttttt tttcccccgc ggtatgactt tctctctccg
     6301 gtacggggag tctatctgga tggggatctt ctgtggacct ttttgacagc agcagagcag
     6361 ctaaaggctc ccttctcatc ctctggcacg cacaacccct gtccatctct aacccaagag
     6421 tcccatctgt gctgagttaa atgctttgcc cccacaaagt gatgattcca aactcaggtt
     6481 atcctgaaca gtcatcccct tgggcccagc acttggaatt tgaaggactc cgtccttgct
     6541 tgccaccccc gacaaagaca gcacttgatt ttctgcactg agttccagat attctgcctc
     6601 tactctgcat tgccctcaac cagtgagctc tgcccacctg gcgtctctgc agtcttaata
     6661 gtgcccagct ggctcaagca agcatcagat caggtacagg agaccctctc agagtcagga
     6721 agctggacca ggtgtgagtt caaaggtctt ggccactgga gaaccagacc attaacttgg
     6781 ctctcttgcc tgttgctgtg caggatagag gggcagggca gaggttcagc cacgattgtt
     6841 tcccaatgat actttccatt gtttgcactt gcctcctcaa atagaaattc attcatcggg
     6901 aggccaccta gcttgcattc cctctagccc cgggcaaggc cacacttagg ccctgtcatt
     6961 gctgccacac ctaggatgat caggtaggaa acgcagggct tcactgtcct gcaggaaggt
     7021 agatcctgca tgagtaagac cccgtacatc cctgtgcaaa cccagggtgt aaacctcagt
     7081 aatgggagag catgacatgg ccaagcctgt ggggccctac tctccaaggc cggggagagc
     7141 aggtaatcta cccagaccca gactgacctg acccctctgc ctcccagggt ccagacccag
     7201 agctcttggc ttcctctccc tcatctgcct ccacagtgag tgtctgcgaa gcacttgctt
     7261 aaatacatat cacgtgtttt agactcgaag ctcaaagcac tggattgtgg tccctgtccc
     7321 tgcccaagtg gctgagacct actgagctgt tcactgtgcc gttcgaggta gggaagaaaa
     7381 ggcctccgag ctgttgctgg gacaggaggg tggagaggcc tgggacagac agcgctccct
     7441 cctgccagta gacactggga catccctgtc cacggagatc agccgggtat cgaaagccat
     7501 gaagactgtc cctagccctg agcgatactg tgacaagaca ttcttgaatt ctccccagac
     7561 aaggtcactt cccccaccat tccagccatt gtctgtctgt caccaattca catcctcact
     7621 tctaggaggg ggaaaggttg agtctttaga tgtgcgattg atccccttgc aactggggga
     7681 agctgggggt ttcctcagtg tctcccacag cgcagctact ctcttcagtg tggggaaagg
     7741 ggggccctgg tgtcagaata cgtaacttaa agcctgtgcg tgtggtggtg cgtggtgtgt
     7801 gtgtgtgtgc atgtgtgctg tatgtgtgta ctttgaacca atagtatata aatataaata
     7861 taaatatata tatatatccc tgacacccgc gagaatctcc accctgtatg agagttgcca
     7921 ttgttacatt cagatcggat tcagttcgtt tctgtttctg tttttgttgt tgttgggttt
     7981 tttttttttt ccttttagaa ctatatagtg ttgagaaagg aatcaaatgt aaatgtctgg
     8041 tttccatata acgttaaaaa agaaaattaa aaaaccatta aggagggggc tggtgtctgc
     8101 cctgccccct tccctgtaaa ttgctgttgt gttgataaag ctgtgcatgg ctcgtgttta
     8161 gcggtcatta ttgtgtctgt ttgtggaatt cttatgatga ggaaaatccc gtagatgcac
     8221 ttattgaata tgtgattagg tctaagtcag ggccaggagt cccaaaacgc ccacaagaaa
     8281 aagataaaaa gaaaaaaaaa tggaccacag ttcctggtga ctatggaaat cgccgtgaga
     8341 gaatcgtgaa tcgagaagac gcggcttgag aatagtcaaa tgtgattttt tttttttttt
     8401 tttttttttg cagatctcat tctgaggctt atggcaaaga caaatgtact tgtgtagagg
     8461 tttctgtgag gtaaaagcca ggagggcctg gctttgaggt caatgctgta gaataggact
     8521 gactggatac caaatgtaat ctttccgatg ctacaatgaa tttatacacg agattgatat
     8581 gcaataaatc tgtgtgtttt tc
//
