LOCUS       NM_001177995            8433 bp    mRNA    linear   ROD 31-OCT-2023
DEFINITION  Mus musculus PR domain containing 16 (Prdm16), transcript variant
            2, mRNA.
ACCESSION   NM_001177995
VERSION     NM_001177995.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Hu T, Wu Q, Yao Q, Yu J, Jiang K, Wan Y and Tang Q.
  TITLE     PRDM16 exerts critical role in myocardial metabolism and energetics
            in type 2 diabetes induced cardiomyopathy
  JOURNAL   Metabolism 146, 155658 (2023)
   PUBMED   37433344
  REMARK    GeneRIF: PRDM16 exerts critical role in myocardial metabolism and
            energetics in type 2 diabetes induced cardiomyopathy.
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Kramer RJ, Fatahian AN, Chan A, Mortenson J, Osher J, Sun B, Parker
            LE, Rosamilia MB, Potter KB, Moore K, Atkins SL, Rosenfeld JA,
            Birjiniuk A, Jones E, Howard TS, Kim JJ, Scott DA, Lalani S,
            Rouzbehani OMT, Kaplan S, Hathaway MA, Cohen JL, Asaki SY, Martinez
            HR, Boudina S and Landstrom AP.
  TITLE     PRDM16 Deletion Is Associated With Sex-dependent Cardiomyopathy and
            Cardiac Mortality: A Translational, Multi-Institutional Cohort
            Study
  JOURNAL   Circ Genom Precis Med 16 (4), 390-400 (2023)
   PUBMED   37395136
  REMARK    GeneRIF: PRDM16 Deletion Is Associated With Sex-dependent
            Cardiomyopathy and Cardiac Mortality: A Translational,
            Multi-Institutional Cohort Study.
REFERENCE   3  (bases 1 to 8433)
  AUTHORS   Zhao S, Nie T, Li L, Long Q, Gu P, Zhang Y, Sun W, Lin Z, Liu Q, Qi
            Y, Wang W, Xie M, Loomes K, Cai C, Wu D and Hui HX.
  TITLE     Androgen Receptor is a Negative Regulator of PRDM16 in Beige
            Adipocyte
  JOURNAL   Adv Sci (Weinh) 10 (21), e2300070 (2023)
   PUBMED   37211698
  REMARK    GeneRIF: Androgen Receptor is a Negative Regulator of PRDM16 in
            Beige Adipocyte.
REFERENCE   4  (bases 1 to 8433)
  AUTHORS   Park A, Kim KE, Park I, Lee SH, Park KY, Jung M, Li X, Sleiman MB,
            Lee SJ, Kim DS, Kim J, Lim DS, Woo EJ, Lee EW, Han BS, Oh KJ, Lee
            SC, Auwerx J, Mun JY, Rhee HW, Kim WK, Bae KH and Suh JM.
  TITLE     Mitochondrial matrix protein LETMD1 maintains thermogenic capacity
            of brown adipose tissue in male mice
  JOURNAL   Nat Commun 14 (1), 3746 (2023)
   PUBMED   37353518
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8433)
  AUTHORS   Thompson M, Sakabe M, Verba M, Hao J, Meadows SM, Lu QR and Xin M.
  TITLE     PRDM16 regulates arterial development and vascular integrity
  JOURNAL   Front Physiol 14, 1165379 (2023)
   PUBMED   37324380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8433)
  AUTHORS   Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier
            G, Langin D and Spiegelman BM.
  TITLE     Transcriptional control of brown fat determination by PRDM16
  JOURNAL   Cell Metab 6 (1), 38-54 (2007)
   PUBMED   17618855
REFERENCE   7  (bases 1 to 8433)
  AUTHORS   Warner DR, Horn KH, Mudd L, Webb CL, Greene RM and Pisano MM.
  TITLE     PRDM16/MEL1: a novel Smad binding protein expressed in murine
            embryonic orofacial tissue
  JOURNAL   Biochim Biophys Acta 1773 (6), 814-820 (2007)
   PUBMED   17467076
  REMARK    GeneRIF: PRDM16/MEL1 is a Smad binding protein that may be
            important for development of orofacial structures through
            modulation of the TGFbeta signaling pathway.
REFERENCE   8  (bases 1 to 8433)
  AUTHORS   Du Y, Jenkins NA and Copeland NG.
  TITLE     Insertional mutagenesis identifies genes that promote the
            immortalization of primary bone marrow progenitor cells
  JOURNAL   Blood 106 (12), 3932-3939 (2005)
   PUBMED   16109773
REFERENCE   9  (bases 1 to 8433)
  AUTHORS   Nishikata I, Sasaki H, Iga M, Tateno Y, Imayoshi S, Asou N,
            Nakamura T and Morishita K.
  TITLE     A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
            expressed mainly in t(1;3)(p36;q21)-positive AML and blocks
            G-CSF-induced myeloid differentiation
  JOURNAL   Blood 102 (9), 3323-3332 (2003)
   PUBMED   12816872
REFERENCE   10 (bases 1 to 8433)
  AUTHORS   Herron BJ, Lu W, Rao C, Liu S, Peters H, Bronson RT, Justice MJ,
            McDonald JD and Beier DR.
  TITLE     Efficient generation and mapping of recessive developmental
            mutations using ENU mutagenesis
  JOURNAL   Nat Genet 30 (2), 185-189 (2002)
   PUBMED   11818962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627127.20, BC053082.1 and AL627123.13.
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_001177995.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region and lacks an alternate exon in
            the 3' coding region compared to variant 1. The resulting protein
            (isoform 2) is shorter and has a distinct C-terminus, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053082.1, SRR13861889.80232.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AL627127.20        39644-39708         c
            66-2339             BC053082.1         1-2274
            2340-2340           AL627123.13        174742-174742       c
            2341-2796           BC053082.1         2275-2730
            2797-2797           AL627123.13        171292-171292       c
            2798-4282           BC053082.1         2732-4216
            4283-8433           AL627123.13        149763-153913       c
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.96 cM"
     gene            1..8433
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /note="PR domain containing 16"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
     exon            1..151
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3648
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /EC_number="2.1.1.367"
                     /note="isoform 2 is encoded by transcript variant 2;
                     transcription factor MEL1; MDS1/EVI1-like gene 1; PR
                     domain-containing protein 16; PR domain zinc finger
                     protein 16; line 27; histone-lysine N-methyltransferase
                     PRDM16"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase PRDM16
                     isoform 2"
                     /protein_id="NP_001171466.1"
                     /db_xref="CCDS:CCDS51400.1"
                     /db_xref="GeneID:70673"
                     /db_xref="MGI:MGI:1917923"
                     /translation="MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILS
                     PIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKR
                     KMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKQISEDLGSEKFCVD
                     ANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSL
                     GVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGV
                     GSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHD
                     SGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHST
                     VKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGK
                     NHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPA
                     FPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSN
                     SSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTG
                     SDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEE
                     QLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLY
                     PFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPS
                     SGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLH
                     YAKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAA
                     MKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLR
                     THTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK
                     HEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQ
                     ASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSP
                     TPEPQGVYEDEEDEEPPSLTMGFDHTRRHMQ"
     exon            152..501
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..552
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            553..690
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            691..793
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            794..1004
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1152
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1306
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..2723
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2808
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2978
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            2979..3056
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3057..3226
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3227..3401
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3402..3635
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..8433
                     /gene="Prdm16"
                     /gene_synonym="5730557K01Rik; csp1; mel1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgctctgtg cgcatgtgcg aaggtgtcca aactgacaat gctagggaga tgaagatagt
       61 gtgtagctgc ttctgggctc aaggaggagg agagagattc cgcgagccga caccatgcga
      121 tccaaggcga gggcgaggaa gctagccaaa agtgacggtg acgttgtaaa taatatgtat
      181 gaacctgacc cggacctgct ggccggccag agtgccgagg aggagaccga agacggcatc
      241 ctgtccccca tccccatggg gccaccgtcc cccttcccca ccagcgagga cttcactccc
      301 aaggagggct cgccctatga ggctcctgtc tacattcctg aagacattcc aatcccacca
      361 gacttcgagc tacgagagtc ctccatacca ggagctggcc tggggatctg ggccaagcgg
      421 aagatggaaa tcggggagag gtttggcccc tacgtggtga cgccccgggc cgcactgaag
      481 gaggccgact ttggatggga gcagatgctg acggatacag aggtgtcatc ccaggagagc
      541 tgcatcaaaa agcagatctc tgaagacttg ggtagcgaga agttctgcgt ggatgccaat
      601 caggcggggt ctggcagctg gctcaagtac atccgtgtag cgtgttcctg tgatgaccaa
      661 aacctcgcca tgtgtcagat caacgaacag atttactata aagtcattaa ggacatcgag
      721 cctggagagg aactgttggt gcatgtgaaa gaaggtgcct actccttggg tgtcatggcc
      781 cccagcttgg atgaggaccc cacattccgc tgtgatgagt gtgatgagct cttccagtgc
      841 aggctggacc tgaggcgcca caagaagtac gcgtgcagct ctgcaggagc ccagctctac
      901 gagggcctag gggaggaact caagcccgag ggccttggcg tgggcagcga cgggcaagcg
      961 catgagtgca aggattgcga gcggatgttc cccaacaagt acagcttgga gcaacacatg
     1021 atcgtccaca cggaagagcg tgagtacaaa tgtgaccagt gtcccaaggc cttcaactgg
     1081 aagtccaacc tcatccgcca ccagatgtct cacgacagtg gcaagcgctt cgaatgtgaa
     1141 aactgtgtca aggtgttcac ggaccccagc aacctccagc gtcacatccg ctcacagcat
     1201 gtcggtgccc gggcccatgc ctgccctgac tgtggcaaga ccttcgccac atcctctggc
     1261 ctcaaacagc acaagcatat ccacagcacg gtgaagccat tcatatgcga ggtctgccac
     1321 aagtcctaca cgcagttctc caacctgtgc cggcacaagc ggatgcacgc cgactgcagg
     1381 acgcagatca agtgcaagga ctgtgggcag atgttcagca ctacctcctc cctcaacaag
     1441 catcggagat tctgcgaggg caagaaccat tacacgcctg gcagcatctt caccccaggc
     1501 ctgcccttga cccccagccc catgatggac aagacaaaac cctccccgac cctcaaccac
     1561 gggggcctag gcttcagcga gtacttcccc tccagacctc atcctgggag cctgcccttc
     1621 tcggctgctc ctccggcctt ccccgcactc actccgggct tcccgggcat ctttcctcca
     1681 tccctgtacc cacgaccacc tctgctacct cccacgccgc tgctcaagag ccccctgaac
     1741 cacgcgcagg acgccaagct acccagcccg ctgggaaacc cagccctgcc ccttgtctcc
     1801 gcggtcagca atagcagcca gggtgccaca gcggccaccg ggtcagagga gaaatttgat
     1861 ggccgcttgg aagacgcata tgcggagaag gtcaaaaata ggagccctga catgtcggat
     1921 ggcagtgact ttgaggatat caacaccacg accgggacag acttggacac taccacgggc
     1981 acggggtcag acctggacag cgacctggac agtgacagag acaaaggcaa ggacaagggg
     2041 aagccagtgg agagcaaacc tgagtttggg ggtgcatctg tgccccctgg ggccatgaac
     2101 agtgtggccg aggtaccggc cttctactca cagcattcct tcttcccgcc acccgaggaa
     2161 cagctgctga cggcctcggg agctgccggc gactccatca aggccatcgc gtccatcgcg
     2221 gagaaatact tcggtcctgg cttcatgagc atgcaggaga agaagctggg ctcactaccc
     2281 taccactccg tgttcccctt ccagttcctg cctaactttc cccactccct ctaccccttt
     2341 acggaccgag ccctcgccca caacttgctg gtcaaggctg agccaaagtc accccgggat
     2401 gccctcaagg tgggcggccc cagtgcggag tgccccttcg acctcaccac caaaccaaaa
     2461 gaggccaaac ccgccctgct cgcacccaag gtccccctca tcccctcatc tggcgaggaa
     2521 cagccactgg acctgagcat cggcagcagg gccagggcaa gccagaacgg aggtggccgt
     2581 gagccgcgga agaaccacgt ctacggtgaa cggaagccgg gggtcagcga ggggctgcct
     2641 aaggtgtgcc cagcacagct gccccagcag ccctccttgc attatgctaa gccttcaccg
     2701 ttcttcatgg atcccatcta cagggtagaa aagcggaagg tggcagaccc tgtgggagtc
     2761 ctgaaagaga agtacctgcg gccgtcccca cttctgttcc acccccagat gtcagccata
     2821 gaaaccatga cggagaagct ggagagcttt gcagccatga aggccgactc aggcagctcc
     2881 ctgcagcccc tgcctcacca cccgttcaac ttccgctccc cacccccaac gctctcggat
     2941 cccatcctca ggaaggggaa ggagagatac acgtgcaggt actgtggcaa gatcttcccc
     3001 agatctgcaa atctcacaag acatctgagg acacacacag gggagcagcc atacaggtgc
     3061 aagtactgtg accggtcatt cagcatctcc tccaacctcc agcggcacgt gaggaacatc
     3121 cacaacaaag agaagccgtt caagtgccat ctgtgcaacc gctgcttcgg gcagcagacc
     3181 aacctagacc ggcacctgaa gaagcacgaa cacgagggcg caccagtgag ccagcactcc
     3241 ggggtgctca cgaaccacct gggcaccagc gcctcctccc ccacctccga gtcggacaac
     3301 catgcacttt tagatgagaa ggaagattct tacttctccg agatccgaaa cttcatcgcc
     3361 aacagcgaga tgaaccaggc atccactcga atggacaaac ggcctgagat ccaagacctg
     3421 gacagcaacc caccgtgtcc aggctcagcc agtgcaaagc cagaggacgt agaggaggag
     3481 gaagaggagg agctggagga agaggatgat gacagcttag ccgggaagtc acaggaggac
     3541 acggtgtccc ccacacctga gccccaagga gtctatgaag atgaagagga tgaggaacca
     3601 cccagcctga ccatgggctt tgaccatacc cggaggcata tgcaatgatg ctgtccctct
     3661 ctgaagacac tcctctccac gccccctccc agagctcact ggatgcttgg ttgaacatca
     3721 caggaccctc gtcagagtcc ggagccttta accccatcaa ccacctctga ggtcctggag
     3781 gccccagggc cagagtccaa agccagggta ccagctgcag gagatggaga aggggccagg
     3841 gagcagcccc ccaccctcaa cacctccact ttgcaaagtc cagcttctcc attgaaactc
     3901 agaacccgaa ggtcccttga gtagccgctg gccttcatca cctctcagaa ctggcctcaa
     3961 ggacacgatc tgcagtgggt gcggtgcacg ggccacccag gagctgctca caggagccat
     4021 ggatcagaaa actcgtgggc aagggtgggg tctctatccc agcaggagcc agttggccac
     4081 atccaggcaa ctgcatggta tgaaagagga aatcggaaag acgtgggcaa gtgctatgga
     4141 gagagacctc atcaatgatt tttataatga gaatcacatg attaagcctt ttggtaatct
     4201 tattgactat agagtctatt taagcatgtg ggttttaaaa aaaatagacg gtatttttta
     4261 aaaatcaaaa aaaaaaaaaa aagacttgca aaatgttttt ttaaaagtaa ttttgcatcg
     4321 ctttggaatt tcagcttatt ggcaaaccca cacctgccag gacatcgagt acggctcggg
     4381 tgtgcccttt ataatgtaaa taatggagaa ttttttttct atctctgacc tgtttgtata
     4441 agccaaggtg attccagtgt gcccgacaca ggataagggg catggagcca ctctgctaag
     4501 tcagatgctg actacacaag aattcatgga cgtggtgcct ggctgcaggt ttgcagaggg
     4561 aggccaggag aaagaggtcg ccttgccctg tgtgctctgc agaggaatcc acgggtccca
     4621 cccaagaccc tccccaattg tatggggaag tcgggagcca gtggctcagg tcttccatgc
     4681 tggacaccta aggatcttcc ccttcagcag cagcactggc cttgactgct gctcttctga
     4741 aggcagggct ctgggccatg tggaggcctc cagccttcta ccctccccaa ccctggcagg
     4801 ctgatgccct ccccatggcc actgagtgtg ggacgcctgc ccacctgcta ctccactcca
     4861 ggacaccaag cactacctgt gcctaagact ccagatcaat cacacagtcg ccacttctgc
     4921 tctttgtgtg ggttcctggt gagaatagga cggggacagg aggaagatcc aggggacctg
     4981 ccacagcaaa gaacagagac agtacagacc ccaagaccgg atgcctcact catttgggac
     5041 ggtcagaacg tgtctacttc tctgcttgga tgggctttaa attatcccat aggggccaat
     5101 ttcaaataat ttcttttcct gatggaattt accttaatct gtatataact tgtaattttt
     5161 ttttctaatt catttctttc cttattttat ttcttcctta acagtatttt tggcattgga
     5221 caacttattc tgaagaaatg atgttaatat aagtatgtag tgaaggacca aaccgtgtga
     5281 taaggttgtg tgctgtgtga tcggtaccca gggagcgggg cggtgttaat gtgccaaata
     5341 cccctgtgtc cctccctcca ggagttccac ggtcaccact gcccacttac cagacaatca
     5401 tatgattttg ttaaatttga gtttcagtta cattttaaga caagtgttct atttatttta
     5461 ttgtttggaa ggaggggggc atatcccaac aagataagaa ggaacagaga ccgcctgggt
     5521 tgtcatttaa aactacaaga gattgtgatt gtgtgagcgt cagcaaactc ggagcacgcg
     5581 tgtcatgccg tgggcctgtc agcggttccc tctgggagca acttaaacat ccaagcacag
     5641 atcttttata tttgctaagg ccgttctggg acaaagaccg tcatctttgc tgatgctcag
     5701 tggtccaggc tacgctggga tccgtgatgg ggatatccca gaagacacca ttcccctgca
     5761 ggttgcggtg aatctcagtg acatagcatg gaacagtcag tcctgtgccg tgctcagaag
     5821 cacactctcg agtgcacact ggacggcctg aggcagcagg gacactctgc agaggaactt
     5881 gccatggtcc agtggtaagg attcgtaggt ctaactgctc atgcttctca tgggttcgtg
     5941 acatcatctc catggcgggc caggcctcgg cttgagaatc ccagtttcct ctaggtggga
     6001 gctagtcctg tgtaagttca acctcagata tacatgcccc acttttgtaa accccaaatt
     6061 atatgctttc ttctcagaaa gagcaaggtg tgtgcttttt ttttcccccg cggtatgact
     6121 ttctctctcc ggtacgggga gtctatctgg atggggatct tctgtggacc tttttgacag
     6181 cagcagagca gctaaaggct cccttctcat cctctggcac gcacaacccc tgtccatctc
     6241 taacccaaga gtcccatctg tgctgagtta aatgctttgc ccccacaaag tgatgattcc
     6301 aaactcaggt tatcctgaac agtcatcccc ttgggcccag cacttggaat ttgaaggact
     6361 ccgtccttgc ttgccacccc cgacaaagac agcacttgat tttctgcact gagttccaga
     6421 tattctgcct ctactctgca ttgccctcaa ccagtgagct ctgcccacct ggcgtctctg
     6481 cagtcttaat agtgcccagc tggctcaagc aagcatcaga tcaggtacag gagaccctct
     6541 cagagtcagg aagctggacc aggtgtgagt tcaaaggtct tggccactgg agaaccagac
     6601 cattaacttg gctctcttgc ctgttgctgt gcaggataga ggggcagggc agaggttcag
     6661 ccacgattgt ttcccaatga tactttccat tgtttgcact tgcctcctca aatagaaatt
     6721 cattcatcgg gaggccacct agcttgcatt ccctctagcc ccgggcaagg ccacacttag
     6781 gccctgtcat tgctgccaca cctaggatga tcaggtagga aacgcagggc ttcactgtcc
     6841 tgcaggaagg tagatcctgc atgagtaaga ccccgtacat ccctgtgcaa acccagggtg
     6901 taaacctcag taatgggaga gcatgacatg gccaagcctg tggggcccta ctctccaagg
     6961 ccggggagag caggtaatct acccagaccc agactgacct gacccctctg cctcccaggg
     7021 tccagaccca gagctcttgg cttcctctcc ctcatctgcc tccacagtga gtgtctgcga
     7081 agcacttgct taaatacata tcacgtgttt tagactcgaa gctcaaagca ctggattgtg
     7141 gtccctgtcc ctgcccaagt ggctgagacc tactgagctg ttcactgtgc cgttcgaggt
     7201 agggaagaaa aggcctccga gctgttgctg ggacaggagg gtggagaggc ctgggacaga
     7261 cagcgctccc tcctgccagt agacactggg acatccctgt ccacggagat cagccgggta
     7321 tcgaaagcca tgaagactgt ccctagccct gagcgatact gtgacaagac attcttgaat
     7381 tctccccaga caaggtcact tcccccacca ttccagccat tgtctgtctg tcaccaattc
     7441 acatcctcac ttctaggagg gggaaaggtt gagtctttag atgtgcgatt gatccccttg
     7501 caactggggg aagctggggg tttcctcagt gtctcccaca gcgcagctac tctcttcagt
     7561 gtggggaaag gggggccctg gtgtcagaat acgtaactta aagcctgtgc gtgtggtggt
     7621 gcgtggtgtg tgtgtgtgtg catgtgtgct gtatgtgtgt actttgaacc aatagtatat
     7681 aaatataaat ataaatatat atatatatcc ctgacacccg cgagaatctc caccctgtat
     7741 gagagttgcc attgttacat tcagatcgga ttcagttcgt ttctgtttct gtttttgttg
     7801 ttgttgggtt tttttttttt tccttttaga actatatagt gttgagaaag gaatcaaatg
     7861 taaatgtctg gtttccatat aacgttaaaa aagaaaatta aaaaaccatt aaggaggggg
     7921 ctggtgtctg ccctgccccc ttccctgtaa attgctgttg tgttgataaa gctgtgcatg
     7981 gctcgtgttt agcggtcatt attgtgtctg tttgtggaat tcttatgatg aggaaaatcc
     8041 cgtagatgca cttattgaat atgtgattag gtctaagtca gggccaggag tcccaaaacg
     8101 cccacaagaa aaagataaaa agaaaaaaaa atggaccaca gttcctggtg actatggaaa
     8161 tcgccgtgag agaatcgtga atcgagaaga cgcggcttga gaatagtcaa atgtgatttt
     8221 tttttttttt tttttttttt gcagatctca ttctgaggct tatggcaaag acaaatgtac
     8281 ttgtgtagag gtttctgtga ggtaaaagcc aggagggcct ggctttgagg tcaatgctgt
     8341 agaataggac tgactggata ccaaatgtaa tctttccgat gctacaatga atttatacac
     8401 gagattgata tgcaataaat ctgtgtgttt ttc
//
