LOCUS       NM_028243               2964 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus prolylcarboxypeptidase (angiotensinase C) (Prcp),
            mRNA.
ACCESSION   NM_028243 XM_914187
VERSION     NM_028243.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Nguyen,B.Y., Zhou,F., Binder,P., Liu,W., Hille,S.S., Luo,X., Zi,M.,
            Zhang,H., Adamson,A., Ahmed,F.Z., Butterworth,S., Cartwright,E.J.,
            Muller,O.J., Guan,K., Fitzgerald,E.M. and Wang,X.
  TITLE     Prolylcarboxypeptidase Alleviates Hypertensive Cardiac Remodeling
            by Regulating Myocardial Tissue Angiotensin II
  JOURNAL   J Am Heart Assoc 12 (12), e028298 (2023)
   PUBMED   37318028
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Lu,C., Zhang,J., Wang,B., Gao,Q., Ma,K., Pei,S., Li,J. and Cui,S.
  TITLE     Casein kinase 1alpha is required to maintain murine hypothalamic
            pro-opiomelanocortin expression
  JOURNAL   iScience 26 (5), 106670 (2023)
   PUBMED   37168577
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2964)
  AUTHORS   Bruschetta,G., Jin,S., Liu,Z.W., Kim,J.D. and Diano,S.
  TITLE     MC4R Signaling in Dorsal Raphe Nucleus Controls Feeding, Anxiety,
            and Depression
  JOURNAL   Cell Rep 33 (2), 108267 (2020)
   PUBMED   33053350
REFERENCE   4  (bases 1 to 2964)
  AUTHORS   Serfozo,P., Wysocki,J., Gulua,G., Schulze,A., Ye,M., Liu,P.,
            Jin,J., Bader,M., Myohanen,T., Garcia-Horsman,J.A. and Batlle,D.
  TITLE     Ang II (Angiotensin II) Conversion to Angiotensin-(1-7) in the
            Circulation Is POP (Prolyloligopeptidase)-Dependent and ACE2
            (Angiotensin-Converting Enzyme 2)-Independent
  JOURNAL   Hypertension 75 (1), 173-182 (2020)
   PUBMED   31786979
REFERENCE   5  (bases 1 to 2964)
  AUTHORS   Danyukova,T., Ariunbat,K., Thelen,M., Brocke-Ahmadinejad,N.,
            Mole,S.E. and Storch,S.
  TITLE     Loss of CLN7 results in depletion of soluble lysosomal proteins and
            impaired mTOR reactivation
  JOURNAL   Hum Mol Genet 27 (10), 1711-1722 (2018)
   PUBMED   29514215
REFERENCE   6  (bases 1 to 2964)
  AUTHORS   Jeong,J.K., Szabo,G., Kelly,K. and Diano,S.
  TITLE     Prolyl carboxypeptidase regulates energy expenditure and the
            thyroid axis
  JOURNAL   Endocrinology 153 (2), 683-689 (2012)
   PUBMED   22202165
  REMARK    GeneRIF: This study showed that PRCP mutant mice have a significant
            decrease in fat mass, although an increase in lean mass was also
            observed; reduced leptin levels and increased energy expenditure
            were also found.
REFERENCE   7  (bases 1 to 2964)
  AUTHORS   Graham,T.H., Shen,H.C., Liu,W., Xiong,Y., Verras,A., Bleasby,K.,
            Bhatt,U.R., Chabin,R.M., Chen,D., Chen,Q., Garcia-Calvo,M.,
            Geissler,W.M., He,H., Lassman,M.E., Shen,Z., Tong,X., Tung,E.C.,
            Xie,D., Xu,S., Colletti,S.L., Tata,J.R., Hale,J.J., Pinto,S. and
            Shen,D.M.
  TITLE     The discovery of non-benzimidazole and brain-penetrant
            prolylcarboxypeptidase inhibitors
  JOURNAL   Bioorg Med Chem Lett 22 (1), 658-665 (2012)
   PUBMED   22079761
  REMARK    GeneRIF: analysis of non-benzimidazole and brain-penetrant
            prolylcarboxypeptidase inhibitors
REFERENCE   8  (bases 1 to 2964)
  AUTHORS   Adams,G.N., LaRusch,G.A., Stavrou,E., Zhou,Y., Nieman,M.T.,
            Jacobs,G.H., Cui,Y., Lu,Y., Jain,M.K., Mahdi,F., Shariat-Madar,Z.,
            Okada,Y., D'Alecy,L.G. and Schmaier,A.H.
  TITLE     Murine prolylcarboxypeptidase depletion induces vascular
            dysfunction with hypertension and faster arterial thrombosis
  JOURNAL   Blood 117 (14), 3929-3937 (2011)
   PUBMED   21297000
  REMARK    GeneRIF: Murine prolylcarboxypeptidase depletion induces vascular
            dysfunction with hypertension and faster arterial thrombosis.
REFERENCE   9  (bases 1 to 2964)
  AUTHORS   Wallingford,N., Perroud,B., Gao,Q., Coppola,A., Gyengesi,E.,
            Liu,Z.W., Gao,X.B., Diament,A., Haus,K.A., Shariat-Madar,Z.,
            Mahdi,F., Wardlaw,S.L., Schmaier,A.H., Warden,C.H. and Diano,S.
  TITLE     Prolylcarboxypeptidase regulates food intake by inactivating
            alpha-MSH in rodents
  JOURNAL   J Clin Invest 119 (8), 2291-2303 (2009)
   PUBMED   19620781
  REMARK    GeneRIF: PRCP is an important component of melanocortin signaling
            and weight maintenance via control of active alpha-MSH1-13 levels.
REFERENCE   10 (bases 1 to 2964)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158138.13 and AK051677.1.
            
            On Jul 29, 2009 this sequence version replaced NM_028243.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051677.1, BC026424.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC158138.13        103960-104003       c
            45-2325             AK051677.1         2-2282
            2326-2964           AC158138.13        44675-45313         c
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 51.64 cM"
     gene            1..2964
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /note="prolylcarboxypeptidase (angiotensinase C)"
                     /db_xref="GeneID:72461"
                     /db_xref="MGI:MGI:1919711"
     exon            1..248
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1562
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /EC_number="3.4.16.2"
                     /note="lysosomal Pro-X carboxypeptidase; proline
                     carboxypeptidase"
                     /codon_start=1
                     /product="lysosomal Pro-X carboxypeptidase precursor"
                     /protein_id="NP_082519.1"
                     /db_xref="CCDS:CCDS21452.1"
                     /db_xref="GeneID:72461"
                     /db_xref="MGI:MGI:1919711"
                     /translation="MGCRALLLLSFLLLGAATTIPPRLKTLGSPHLSASPTPDPAVAR
                     KYSVLYFEQKVDHFGFADMRTFKQRYLVADKHWQRNGGSILFYTGNEGDIVWFCNNTG
                     FMWDVAEELKAMLVFAEHRYYGESLPFGQDSFKDSQHLNFLTSEQALADFAELIRHLE
                     KTIPGAQGQPVIAIGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGMVPCGEFMK
                     IVTNDFRKSGPYCSESIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSEKIPTLKGW
                     IAETWVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYN
                     YSGQAACLNISQTTTSSLGSMGWSFQACTEMVMPFCTNGIDDMFEPFLWDLEKYSNDC
                     FNQWGVKPRPHWMTTMYGGKNISSHSNIIFSNGELDPWSGGGVTRDITDTLVAINIHD
                     GAHHLDLRAHNAFDPSSVLLSRLLEVKHMKKWILDFYSNIQ"
     exon            249..389
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            390..491
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            492..673
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            674..831
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            832..1001
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1166
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1354
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..2964
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1558..1563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /note="hexamer: AATGAA"
     polyA_site      1580
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
     regulatory      2369..2374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /note="hexamer: AATAAA"
     polyA_site      2394
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /note="major polyA site"
     regulatory      2941..2946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
                     /note="hexamer: AATACA"
     polyA_site      2962
                     /gene="Prcp"
                     /gene_synonym="2510048K03Rik; 2610104A14Rik; HUMPCP; PCP"
ORIGIN      
        1 tacgagctgg gcccggctta gtctttgcgc ttctgctggt ggctctcttt ctttagtcgc
       61 ccacactgtg gctgccatcc ccagccatgg gctgccgggc tctcctgctc ctgtctttcc
      121 tattactcgg ggccgccaca accatcccgc ctaggctaaa gaccctcggc agcccgcatt
      181 tgtcagccag tcccacaccc gaccctgctg tagcccgtaa atactcagtt ctttacttcg
      241 aacagaaggt tgaccacttt gggtttgctg acatgagaac ttttaaacag cggtacctag
      301 tcgctgataa acactggcag agaaatggtg gatcaatact tttctacact ggtaacgaag
      361 gggacattgt ctggttttgc aataatacgg ggttcatgtg ggatgtggct gaggagctga
      421 aagctatgct ggtgttcgct gagcatcgat actatgggga gtcccttccc tttggtcagg
      481 actccttcaa ggattcccaa cacttgaatt tcctgacatc agaacaggct ctggctgatt
      541 ttgcagagtt aatcagacac ttggaaaaaa caatcccagg agctcaaggt caacctgtca
      601 tcgccatcgg gggctcttat ggtggcatgc ttgcagcctg gttcaggatg aagtatcctc
      661 acattgtagt tggagctctt gcagcttctg cccctatctg gcagcttgat ggtatggttc
      721 cctgtggtga gtttatgaag attgtaacta acgatttcag gaaaagtggt ccttattgtt
      781 cagaaagtat ccgcaaatcc tggaatgtca ttgacaaact ctcaggttca ggcagtggtc
      841 tccagtcgct cacgaacatt cttcacctat gtagtccctt gacttctgag aaaatcccaa
      901 ctttaaaagg atggatagct gagacctggg tgaacctggc catggtgaac tacccttacg
      961 catgcaactt tttgcagcct ttgcctgctt ggcccatcaa ggaggtgtgc caatatttga
     1021 aaaatcctaa tgtatcggat acagtgctgc tgcagaatat tttccaagct ctgagtgtat
     1081 attataacta ttcaggccaa gcagcatgcc tgaatatttc acaaacgact acgagcagtc
     1141 tgggctccat gggctggagc tttcaggcct gcacagaaat ggtcatgccc ttttgtacta
     1201 atggtattga tgacatgttt gaaccctttc tgtgggactt ggagaagtat tccaatgact
     1261 gttttaacca gtggggagtg aaaccacggc ctcattggat gactaccatg tacggtggca
     1321 aaaacatcag ttcacactca aacatcattt tcagcaatgg tgagttagac ccgtggtcag
     1381 gaggtggagt aaccagagac atcacagaca ccctggtggc tatcaacatc catgacgggg
     1441 cccatcattt agatctccga gcccacaacg ccttcgatcc ttcctctgtg ctgttatctc
     1501 gattattgga agttaaacac atgaagaaat ggattttaga tttctatagc aatatccaat
     1561 gaacaatttt ttaaaattaa tttttttttg ttttctttta tgttccctcc acccacatac
     1621 ccattcactt ttatgtaatt cacttatatg taattacttt ctctcatttg tcattatatt
     1681 tgttgagcca aggccaagac acaagagagt gacaggggtg ggcacagaca tcccacccag
     1741 gtgactgctc cggcctcact gtctttgtgt catcatggca gctcacaggc catcagaact
     1801 ggcatggctc ctcactgcct ttcaccagag ggagagcccc tctcccagca gcagggaggt
     1861 ctccttggtt ctgaggctga agtctctctg gcccattgta agtccccact cctgccacaa
     1921 aagggaaagc agaagccggg ttcaaaacgc tgcagctgca gctgatagaa tgtggaatgc
     1981 cagtccagga aaccgggctg cttcctgtga gctcactcag tgagtgattc tgaactgggc
     2041 tgccataaag aagaaggcag agacctacct gcctgttgct tctgtctcct tccagctgtt
     2101 gggaggtcag tgtgcctggg ctggctgctt ttggaaagtc ctttctttat ctctttccca
     2161 cttggcagct ctccttactt ctgggacatt tgccactgtg gtactgccag gaaggaatct
     2221 ggccttgtcc tgctctcttt gtttccaagg ccaattttat ggtggtctgg ctacattctg
     2281 ccaatttagg aaaagtgcta tttttgtttt ttggtttttt tttttgtttt gttttgtttt
     2341 tttactttaa aactgaacaa attctgttaa taaaaattct ttaaaaattg caaaccattt
     2401 attgaaaaag tagaatgtac ttctgttcat ttggtaaaga aattttatgt tctagtcatt
     2461 gttaccagta tcattatagc cttaggtgtc tggattccta gcatcaggtg tgtggggact
     2521 ttcctgtgtg gcttctagaa gacaagcaga ctaattcaag caggcagcat atttggcatt
     2581 aatagatgtc agagccttcc catctccaag gcttggaact tgaagcagtt cttacagtga
     2641 gacctttttt ctaagggacg tttccagcct aggaaataag gttgaatcca aacctccgag
     2701 ggacacagcc taacaggttc ttctcaacag cagcccccca gtttctttat ttcttagtca
     2761 tgttcttacc atggcttgga aacatttctc gcttttcaga aactatccct agagccctga
     2821 taagatttta ttttattatt gctttcttgg tttcgagatt ataatataat tacaattaca
     2881 tttctgtttt tctctttcct ccctttaaat tgtcccatat accccacctt gctctcttta
     2941 aatacatggc ctttttaaaa aatt
//
