LOCUS       NM_172911               4780 bp    mRNA    linear   ROD 19-DEC-2022
DEFINITION  Mus musculus PEAK1 related kinase activating pseudokinase 1
            (Prag1), mRNA.
ACCESSION   NM_172911
VERSION     NM_172911.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Weaver KL, Alves-Guerra MC, Jin K, Wang Z, Han X, Ranganathan P,
            Zhu X, DaSilva T, Liu W, Ratti F, Demarest RM, Tzimas C, Rice M,
            Vasquez-Del Carpio R, Dahmane N, Robbins DJ and Capobianco AJ.
  TITLE     NACK is an integral component of the Notch transcriptional
            activation complex and is critical for development and
            tumorigenesis
  JOURNAL   Cancer Res 74 (17), 4741-4751 (2014)
   PUBMED   25038227
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Tanaka H, Katoh H and Negishi M.
  TITLE     Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity
  JOURNAL   J Biol Chem 281 (15), 10355-10364 (2006)
   PUBMED   16481321
REFERENCE   3  (bases 1 to 4780)
  AUTHORS   Caenepeel S, Charydczak G, Sudarsanam S, Hunter T and Manning G.
  TITLE     The mouse kinome: discovery and comparative genomics of all mouse
            protein kinases
  JOURNAL   Proc Natl Acad Sci U S A 101 (32), 11707-11712 (2004)
   PUBMED   15289607
  REMARK    GeneRIF: Also known as SgK223, a member of the NKF3 family of
            protein kinases predicted to be catalytically inactive.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121858.3, AK052494.1, BC089024.1, AK036672.1 and CO042744.1.
            
            [WARNING] On Dec 20, 2023 this sequence was replaced by
            NM_172911.4.
            
            On Nov 29, 2007 this sequence version replaced NM_172911.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036672.1, SRR9219379.30216.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC121858.3         114674-114706
            34-542              AK052494.1         2-510
            543-2801            BC089024.1         1-2259
            2802-4537           AK036672.1         2770-4505
            4538-4780           CO042744.1         18-260              c
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 22.78 cM"
     gene            1..4780
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /note="PEAK1 related kinase activating pseudokinase 1"
                     /db_xref="GeneID:244418"
                     /db_xref="MGI:MGI:1196223"
     exon            1..161
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
     exon            162..579
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..4410
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /EC_number="2.7.10.2"
                     /note="pragmin; tyrosine-protein kinase SgK223; sugen
                     kinase 223; tyrosine-protein kinase PRAG1; notch
                     activation complex kinase"
                     /codon_start=1
                     /product="inactive tyrosine-protein kinase PRAG1"
                     /protein_id="NP_766499.2"
                     /db_xref="CCDS:CCDS22247.2"
                     /db_xref="GeneID:244418"
                     /db_xref="MGI:MGI:1196223"
                     /translation="MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAG
                     TRLPARPENCRLDDEGVNGLAYSKPTIAVKPTMMTSETSDLWTEASLSAEVPKVNWRR
                     TPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNSRCPPAYTMVGLHNLEARVDR
                     NTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSPQPLRESLP
                     SEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRT
                     RPTEEEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSP
                     FAPHLENDYCSLVKEPASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYA
                     ESAKRKKAAPGPPRPEPKKEQVPAGHSQGQVWTGDTWIQKTPPSWSQDREGANPAPQV
                     ATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDLQAGEEEPLVAQGLTSRES
                     HPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQLLSRVP
                     ANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQE
                     LSQSWGRELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGR
                     LEAFSPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLAS
                     DSRPCSDGGPSYEPTHSPTISGKKLFAPVPFPSGSTEDVSPGGGPAQPPPLPQKKIVS
                     RAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPFNCSLNSGNRSHHVFSS
                     SEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSSSTQL
                     QLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKL
                     TCNKPCCDSGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCG
                     HFVASVPSSMLASPDTSSKDTAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMA
                     SHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLAHCNPQSSPGPSAT
                     PTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQKPGGTTNLQQKKSQA
                     RLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSL
                     YSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHN
                     WIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL"
     exon            580..2375
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2533
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..3294
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..4780
                     /gene="Prag1"
                     /gene_synonym="9830148H23; D8Ertd82e; mFLJ00269; NACK;
                     Sgk223"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atcgcagctg ccacccggcc gccgggccca ggcagaagtc agccgcccgc ggctgggcaa
       61 gcactccgcg cagacacgct aaagacaatg ctccgaggcg tccggcgggg caacggactg
      121 ctgggtcacc gacggcgagg ctgcgagacc gcagacccca gggatttgat ctggaactca
      181 tcgctgcctc ctgcaagacg gcaatcagaa agcctggccc aagatgaact cgaaccttat
      241 cggctcacag tgctccagac catttgactg aaccccgaga gcccaaaaat gtctgcgtgc
      301 agcgactttg tggagcacat ctggaaaccc gggtcttgca agaactgctt ctgcttgcga
      361 agtgatcatc agccaacagc tggccatccc aaggctagag ccaacagcct acccgctgga
      421 actcgcctgc ctgccaggcc tgagaattgc cgcctagacg acgaaggcgt gaatggcttg
      481 gcctactcaa agcccaccat tgctgtaaag cccaccatga tgacttctga gacctccgac
      541 ttgtggactg aagccagcct gagcgctgaa gtcccaaagg tcaattggag acgaacccca
      601 ggcaagctcc tgctcccgaa acaggaagat gagccaatag tttacctagg cagcttccga
      661 ggactgcaga agcctgcaag ccctcttgcc tgcacagatg gcaactctcg ttgtccccca
      721 gcctatacca tggtcggact gcataacctg gaggcccgcg tggataggaa cactgccttt
      781 caaccagtga gcttccagga agagaaggct gggagggaag agctgccctc agctcacgag
      841 agctttcgcc agaagctggc tgcctttgca gggatgacat ccagctgccc caagggcccc
      901 aggccctgca cttctccaca gcccctgcgg gaatccctgc cctcggagga tgacagtgac
      961 caaaggtgct caccctcagg agacagcgaa ggtggggagt actgttccat tctggactgt
     1021 tgtccagaat ccaaagatgc tgtgcatagc accgagggct ctggcagacg tggaggggac
     1081 tgctcaccca cgtgcaggga gcagggacca cgtacaagac caacagaaga ggaaaaacag
     1141 ggtctgagct tccccagaga gtgctgtggt cagggatcca cagcgaaccc gccccgcctg
     1201 ggccccaaga agccatccct taactctgag gccgccagct cttccgatgg tctgtcctgt
     1261 ggcagcagcc gcagcggggc cagcagcccc ttcgctcccc accttgagaa cgactactgc
     1321 tctcttgtga aggaaccggc ctcagggaag cagcaagacc tcagcggcca ctttctcacc
     1381 tctggcaaat gtgtgggtca agctgcggaa ctccagcccg cctcccttct tagggaccct
     1441 gtgcagcctg aacccatcta cgccgaaagc gccaagagga agaaggcagc tccggggcct
     1501 ccgaggcccg agcccaagaa agagcaggtc ccagctggcc atagccaggg ccaagtatgg
     1561 acaggtgata cctggattca gaagacaccc cctagctgga gccaggacag ggaaggcgca
     1621 aaccctgctc ctcaggtggc aaccaccatc actgtcatcg ctgctcaccc ggaagaggac
     1681 cacaggacta tttatctgag cagccctgac tctgccgtgg gggtgcagtg gccacgtggg
     1741 ccttcgaacc aggacttaca agccggtgaa gaggagcctt tggttgcgca ggggctaacc
     1801 tcaagggaga gccatccaca taatgtgact gaaaacacgg ccaaagagaa gcctgccatt
     1861 ccccccaaac tgtccaaaag cagccccgga gggtcccctg tgtctcccgc gcccccactg
     1921 actgaccaca gcgacgggaa caccggtggc agcagcgtcg gaccccaact tttgtctaga
     1981 gtccctgcta acctaacgtc ttcctgccac accaatggcg ttgccactgc tggtgactct
     2041 gccaagtgtc ccccaccagc cacctcctct tcagtcttgg accagaggcg gccaagatac
     2101 cagactggtg cttggagccg ccagtgccgg atagaagagg aggaggaggt ggggcaggag
     2161 ttgagtcaaa gttgggggag agagttggaa aacggtaccg cagaccattc aaactcctct
     2221 acctggcacc gtctccatcc catcgatggc acctctgggc agaacagcaa aactaactct
     2281 gggatgagca aatcggcttc ctttgcgttt gagttcccaa aggacagagg cagattggag
     2341 gcattctcac cccctccccc acctccaaag tcaaggcacc tgttaaaaat gaacaagagc
     2401 agttctgact tggaaaaagt gagccagagt tctgcagaaa gcctcagccc gtccttcaga
     2461 ggggcccacg tcagcttcac cacgggctcc acagatagcc tagcctcgga ctccaggccc
     2521 tgcagcgatg gaggtccatc atatgaaccc acccactcac ccaccatcag cgggaaaaag
     2581 ctcttcgctc ccgtcccttt tccatcaggc tccacagagg atgtgtcccc tggtggtggt
     2641 cctgcgcagc ctcctcctct gccccagaaa aagatagtga gccgtgccgc ctcttcccct
     2701 gatggcttct tctggaccca aggttcccct aagccacgga cggcaagtcc caagctgaac
     2761 ctaagccatt cagaaaccaa tgtttgtgcc catgacgagc ctccttttaa ctgttccttg
     2821 aactctggaa accgttccca ccatgtcttc tcctcttctg agcctttggg gaaagctttc
     2881 aaaggcaatg ctccctgggc cccagctctg gggctggcga acagcaaagg cggctgtggg
     2941 agccccagtc tccagtgcag agcggccacc tccacgtcat catcccagct cagcgtgtcc
     3001 agccaggcct cctccagcag cacccagctt cagctgcaca gcctgctgag tagcatcagc
     3061 agcaaggagg gcacctatgc caagcttggg ggcctctaca cacaatccct ggcccgccta
     3121 gtgaccaagt gtgaagatct cttcatgggt ggccagaaaa aggaactgcg cttcaatgag
     3181 aattactggt cactcttcaa gctgacgtgc aacaaacctt gctgtgactc gggggatgca
     3241 atttactact gtgccacctg ctctgaggac ccaggcagca tctatgctgt gaaaatctgc
     3301 aaaaccccag aacccaaatc agcctcatac tgcagcccat ctgtgccggt gcatttcaac
     3361 atccagcaag actgcggcca ctttgtggcc tctgtgccct ccagcatgct tgcctcccct
     3421 gacacatcca gcaaagacac cgcgcctgcc gtatccccac agccccctgc ccaggagcag
     3481 gactgtgtgg tggtcatcac ccgggaggta ccgcaccaga ctgcctccga ctttgtgcgg
     3541 gactctatgg ccagccaccg ggcggagcct gaggtttacg agcgccgggt gtgcttcctg
     3601 cttctgcagc tctgcaacgg gctggagcac ctgaaggaac acgggatcat ccaccgggac
     3661 ctgtgtctgg agaacctgct gctggcgcac tgtaaccctc agagctcccc cgggccctct
     3721 gccactccta ccgtgcccac caccacatcc aggtgccctt ctgccgcgcc cgcagccacc
     3781 actgcctgcc agggagggcc tggtgagaag cagctgccca ggctcatcat cagcaacttc
     3841 ctgaaggcca agcaaaagcc aggaggcacc accaacctgc aacagaagaa gagccaggcc
     3901 cggctggctc ccgagatcgt gtctgcctcc cagtaccgca agtttgatga gttccagacg
     3961 ggcatcctca tctatgagct gctccaccag cccaacccat tcgaggtgcg agcccagctc
     4021 cgggagcgcg actaccggcg ggaggactta cccccgctgc ccacactgtc cctctactct
     4081 cccggcctgc agcaactcgc ccatctactg cttgaggctg atcccatcaa gcgcatccgc
     4141 ataggtgaag ctaagcgggt gctgcagtgc ttgctgtggg ggccacggcg ggagctggtg
     4201 gagcagccgt gcacgtcaga ggaggtactg tgtaacactc tgcacaactg gatcgacatg
     4261 aagcgagcac tgatgatgat gaagtttgct gagaaggcgg tggaccgaag gcggggtgtg
     4321 gaactggagg actggctttg ctgccagtac ttggcctcgg cagagccagg ggccctcttg
     4381 cagtccctga agctcctaca acttctctga gccattccat cgccaccgcc ttccacgcct
     4441 gccccatgcc cttgccttgc ctgcatccgg attaccctgg gaaatggtac aaatgactgt
     4501 tgtccttgga tgtaatattt tatatatata catatataat atgaaagact ggggatgtca
     4561 tttcacacac gccagcctcc tctcagagtc cacattcctc caccaccgcc acccctgtga
     4621 ttatgtacag gtctagcttc acacaaggtt ctgagggcag ttccatcccg acagaaccaa
     4681 gttcggactc ctgcctcact agcccatttt ggagtctcct cgcccttttg tcaaactgtt
     4741 atttaaaaaa acaacttcca ataaagaaaa aagtcttata
//
