LOCUS       NM_001163427            1082 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus protease-associated domain containing 1 (Pradc1),
            transcript variant 2, mRNA.
ACCESSION   NM_001163427
VERSION     NM_001163427.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Rodriguez,S., Stewart,A.N., Lei,X., Cao,X., Little,H.C., Fong,V.,
            Sarver,D.C. and Wong,G.W.
  TITLE     PRADC1: a novel metabolic-responsive secretory protein that
            modulates physical activity and adiposity
  JOURNAL   FASEB J 33 (12), 14748-14759 (2019)
   PUBMED   31689374
  REMARK    GeneRIF: PRADC1-deficient mice have reduced adiposity and enhanced
            locomotor activity uncoupled from metabolic health.
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155728.13, AK006658.1 and CJ208123.1.
            
            Transcript Variant: This variant (2) contains an alternate in-frame
            exon in the 3' coding region, compared to variant 1. This results
            in a longer protein (isoform b) compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006658.1, BI526406.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC155728.13        144932-144965       c
            35-928              AK006658.1         1-894
            929-1057            CJ208123.1         250-378
            1058-1082           AC155728.13        140448-140472       c
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 37.46 cM"
     gene            1..1082
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /note="protease-associated domain containing 1"
                     /db_xref="GeneID:73327"
                     /db_xref="MGI:MGI:1920577"
     exon            1..190
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..690
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; protease-associated domain-containing protein
                     of 21 kDa; protease-associated domain-containing protein
                     1"
                     /codon_start=1
                     /product="protease-associated domain-containing protein 1
                     isoform b precursor"
                     /protein_id="NP_001156899.1"
                     /db_xref="CCDS:CCDS51828.1"
                     /db_xref="GeneID:73327"
                     /db_xref="MGI:MGI:1920577"
                     /translation="MSRGAAGWCCLVLWLPTCVAAHGLRIHDYLYFQVLSPGDIRYIF
                     TATPAKDFGGIFHTRYEQIHLVPAEPPEACGELSNGFFIQDQIALVERGGCSFLSKTR
                     VVQEHGGRAVIISDNAVDNDSFYVEMIQDSTQRTADIPALFLLGRDGYMIRRSLEQHG
                     LPWAIISIPVNVTSIPTFELLQPPWTFW"
     exon            191..291
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /inference="alignment:Splign:2.1.0"
     exon            292..401
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /inference="alignment:Splign:2.1.0"
     exon            402..569
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /inference="alignment:Splign:2.1.0"
     exon            570..1082
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1037..1042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /note="hexamer: AATAAA"
     polyA_site      1057
                     /gene="Pradc1"
                     /gene_synonym="1700040I03Rik; Pap21"
                     /note="major polyA site"
ORIGIN      
        1 gttctgtgct ccaaccggcg cggagagcgg gcctgaggga ggccgtggca gctgtcaggc
       61 taaagtcggg cgagggatcg cggcgggacg gcggcggcgg cggcggcggg acgggccctg
      121 gagatgagcc gtggcgccgc gggctggtgc tgtctcgtgc tctggctgcc cacgtgcgtc
      181 gcggcccacg gcttacgcat ccatgactac ttatacttcc aagtgctgag tcctggggat
      241 attcgataca tcttcacagc cacacctgcc aaggactttg gtgggatatt tcacacacgg
      301 tatgagcaga ttcacctcgt ccctgctgaa cctccagagg cctgcgggga actcagcaac
      361 ggcttcttta tccaggacca gatcgctctg gtggaaaggg ggggctgctc cttcctctct
      421 aagaccaggg tggtacagga gcatggtggg cgggctgtga tcatctcgga caatgcagtt
      481 gacaatgaca gtttctacgt ggagatgatc caggatagta ctcaacgcac agctgacatt
      541 cctgccctct tcctgctcgg ccgagatggc tacatgatcc gccgttctct ggaacagcac
      601 gggctaccat gggccatcat ctctatccca gtcaatgtca ccagtatccc cacctttgag
      661 ctactgcaac ctccgtggac tttctggtag aaaagctgac tccaggttcc agtgacaagt
      721 gactcatggg aagaggaaac ccaagattct gctatttgga gtttggagat agattctgag
      781 gacaagtgga accagagagg acagggaacc cctcctcctg gctttccctt ccccagggcc
      841 tcaaggacat ctcatgattc aggaagaggc ttctgactag aatttgaaac aaaaggtgct
      901 gaaggcagat ggccctgata cacaccacct gtttcagctc ccagcagcca agccctcctc
      961 acagtgagcc ttgtagctgc tgctcaagtg gtttaaagaa gtgatatttg gggactaata
     1021 agcccatact ttcagcaata aagccaaaca aagctcacca ggtttgtgcc tgtgtcctaa
     1081 ac
//
