LOCUS       NM_028980               3683 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus protein phosphatase 4, regulatory subunit 4 (Ppp4r4),
            mRNA.
ACCESSION   NM_028980
VERSION     NM_028980.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Cui,W., Dai,X., Marcho,C., Han,Z., Zhang,K., Tremblay,K.D. and
            Mager,J.
  TITLE     Towards Functional Annotation of the Preimplantation Transcriptome:
            An RNAi Screen in Mammalian Embryos
  JOURNAL   Sci Rep 6, 37396 (2016)
   PUBMED   27869233
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
            Goudreault,M. and Gingras,A.C.
  TITLE     PP4R4/KIAA1622 forms a novel stable cytosolic complex with
            phosphoprotein phosphatase 4
  JOURNAL   J Biol Chem 283 (43), 29273-29284 (2008)
   PUBMED   18715871
REFERENCE   3  (bases 1 to 3683)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029499.1, BC111887.1 and BB307572.1.
            
            On Apr 18, 2009 this sequence version replaced NM_028980.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029499.1, SRR1660811.63709.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2337              AK029499.1         2-2338
            2338-2485           BC111887.1         2333-2480
            2486-3600           AK029499.1         2487-3601
            3601-3683           BB307572.1         210-292
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 52.95 cM"
     gene            1..3683
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /note="protein phosphatase 4, regulatory subunit 4"
                     /db_xref="GeneID:74521"
                     /db_xref="MGI:MGI:1921771"
     exon            1..178
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2683
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 4"
                     /protein_id="NP_083256.2"
                     /db_xref="CCDS:CCDS36532.1"
                     /db_xref="GeneID:74521"
                     /db_xref="MGI:MGI:1921771"
                     /translation="MHPPPPDAGVAMDFGQNSLFGYMEDLQELTIIERPVRRSLKTPE
                     EIERLTVDEDLSDIDRAVYLLSAGQDVQGASVIANLPFLMRQNPTETLRRVLPKVREV
                     LHVASVEMQLTAAVSFLTILQEESMSVHTCAHSFLQVILLHLEHRDTGVSNAWLETLL
                     SAVELLPKETLRHEILNPLVSKAQLSQTVQSRLVSCKILGKITNKFDAHSIKREILPL
                     VKSLCQDVEYEVRSCMCRQLENIAQGIGAELTKNVVLPELIELSRDESGSVRLAAFET
                     LVNMLDMFDTDDRSQTILPLVKSFCEKSFKADESILISLSFHLGKLCHGLYGIFTPDQ
                     HLRFLEFYKKLCTLGLQQENGHNESQIPSQIVEQEKKYTSVRKNCAYNFPAMIVFVDP
                     KNFHMELYSTFFCLCHDPEVPVRHTIAICFYEVSKLLNSGVHLIHKELITLLQDESLE
                     VLDALINHLPEILELMSTGGENSVQENKFSSVPDLIPALTAAEQRAAASLKWRTHEKL
                     LQKYTCLPHIISSDQIYYRFLQRMFTIMMTNNVLPVQRAAARTLCIFLRYNRKQEQRH
                     EVIQKLIEQLGQGKSYWNRLRFLDTCEFIIEIFSRSFFCKYFFLPVIELTHDPVANVR
                     MKLCYLLPKVKSALKIPADMHLLQQLEMCVRKLLCQEKDKDVLAIVKKTVLELDRMEM
                     SMDMFQKKNYEKDLLDQEKEREELLFLEMEQLEKEKHQSDGRLASDKSFEKKRRDSRT
                     STQSLSKNLPISVPGPSSSTASTSKEIKKSKLTRSQSFNNQAFHAKYGTLDKCASKSS
                     TLAHTSSVSGLVRTAMLSLTDDSFRTRNASSVPASFSPNPVMPSTSRGPGNTADPKSS
                     GSKDAQPRKATLKSRKSNP"
     exon            179..252
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            253..355
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            356..503
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            504..577
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            578..684
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            685..792
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            793..914
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1037
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1207
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1327
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1405
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1489
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1672
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1778
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1926
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2071
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2113
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2188
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2258
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2345
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2439
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2510
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2658
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..3683
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3661..3666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /note="hexamer: AATAAA"
     polyA_site      3682
                     /gene="Ppp4r4"
                     /gene_synonym="8430415E04Rik; mKIAA1622"
                     /note="major polyA site"
ORIGIN      
        1 gtccggcatc ccggggccgc tcgggcgggc cggcgaggga gcccctcggc ggtccatgca
       61 tccgccgccg cccgacgccg gcgtcgcgat ggatttcggg cagaacagcc tgtttggcta
      121 catggaggac ctgcaggagc tcaccatcat cgagaggccg gtccgccgga gcctcaagac
      181 accagaagaa atagaaagac tgactgttga tgaagatctc agtgatattg acagggctgt
      241 ttatctgctc agtgctggtc aagatgtcca aggagccagt gtgattgcaa accttccatt
      301 tttgatgcga cagaatccta ctgagacact tcggagagtc ttacccaaag tcagagaagt
      361 cctgcatgtc gcaagcgtgg agatgcagct aacggctgca gtgtcctttt tgaccatcct
      421 tcaggaagaa tcaatgtcag tccacacctg tgcacactcc ttcctgcaag tcattctcct
      481 gcacctggag cacagggaca caggtgtcag caatgcatgg ctggagacac ttctctccgc
      541 agtagaatta ttgccaaaag aaaccctgag gcatgagatt ctcaatccac ttgtttccaa
      601 ggcacagctt tcccaaaccg tccagtcgcg tttagttagc tgtaaaattt taggaaaaat
      661 aaccaacaaa tttgatgccc atagcattaa gagagaaata cttcccctgg taaaatcact
      721 ctgtcaagat gtggaatatg aagttcgatc ttgtatgtgt cgccagttag aaaacatagc
      781 tcagggcatt ggggcagaac tgacaaagaa tgtggtgctt cccgaactaa tagaactctc
      841 tagggacgag agtggcagtg tgcggcttgc tgcttttgaa accttggtta acatgcttga
      901 catgttcgat acagatgaca gaagtcaaac tatacttccc ttagtgaaat cgttctgtga
      961 aaagtctttc aaagcagatg aatccattct gatttcttta tcatttcatt taggaaagct
     1021 atgccatgga ctctatggaa ttttcactcc agatcagcac ttgagatttt tggagtttta
     1081 taagaaactt tgtacactgg gtttgcaaca agaaaatggc cacaatgaaa gccagattcc
     1141 atcccaaatc gtagagcagg agaagaaata tacttcagta cgaaagaact gtgcttacaa
     1201 ctttccggcc atgattgttt ttgttgatcc taaaaacttc cacatggaac tctactctac
     1261 attcttctgc ctttgtcatg accctgaagt gccagtcaga cacactattg ctatttgctt
     1321 ttatgaagta tctaaacttc tgaattctgg agtacattta attcacaaag agctgataac
     1381 attgttacaa gatgagtcac tggaggttct agatgctctt ataaatcacc ttccagaaat
     1441 cttggaactt atgtctactg gtggagaaaa cagtgttcaa gaaaataagt tttcctcagt
     1501 gccagacttg attccagcac tcacagctgc tgagcagcga gctgcagcct ccctgaagtg
     1561 gaggacccac gagaagctgc tgcagaagta cacctgtctg cctcatatca tatcgagtga
     1621 ccagatttat taccgtttct tacaaagaat gttcaccatc atgatgacaa ataatgtctt
     1681 acctgtccaa agggcagctg cacgaactct atgcattttt ctacgatata atcgtaaaca
     1741 agagcagagg catgaagtca ttcaaaaact aattgaacag ttgggccaag gaaaaagtta
     1801 ttggaataga cttcgctttt tggatacctg tgaatttatc atagagatat tttccagatc
     1861 atttttctgc aaatatttct ttctacctgt tattgaacta acccatgacc cagtggcaaa
     1921 tgtgagaatg aaactttgct acctgctgcc caaggtgaag tctgcgctga agattcctgc
     1981 agacatgcat ctcctccagc agctagaaat gtgtgtgaga aagctcctgt gtcaagaaaa
     2041 agataaagac gtcctggcta ttgtgaagaa aacggtatta gagttggaca gaatggagat
     2101 gtctatggat atgttccaga aaaaaaacta tgagaaagat ttgttggatc aagagaaaga
     2161 aagagaagag ctgctgtttt tggaaatgga acagctggag aaggagaagc atcagagtga
     2221 cgggaggctg gccagtgaca aaagctttga gaagaaacgc agagactcca ggacatcaac
     2281 tcagagcctg tccaagaact tgcccatttc tgttcctgga ccctcctcta gcactgcatc
     2341 aacaagcaaa gaaatcaaga aatccaaatt gactcgaagc caatctttta ataatcaagc
     2401 ttttcatgca aaatatggca ccttagacaa gtgtgctagt aaaagttcca ccttagcaca
     2461 cacgtcttcc gtctcagggc tggtgaggac tgccatgctg tcactaactg atgactcatt
     2521 ccgaactcga aatgccagca gtgttccagc ttccttttct cctaaccctg tcatgcccag
     2581 cacttcccgt gggccaggta acacagccga tccaaagagt agtggaagta aagatgcaca
     2641 gccccggaag gctaccttaa aatccagaaa atccaatcct taagtcaact gcttggtgaa
     2701 ggagacaagc agtcagcgga gaatctgatg gtggaaaagc tacagcgaca gacggccagg
     2761 attgttaaag tgtggaaggg aggaaggaac atcgtgcagc tgatgtgaaa cttgccctat
     2821 ttggaattag atttccctag gaggtatgac atgcgattta ctgtaggctc tgaatccgag
     2881 tgctccagtg cagtgtaatg agctccaact tggtgtgtac tgcctgctgg actgaagaga
     2941 catgcctgtc tttatgtgag aggctggctt ttgtttacta agttgtagca tttagacatc
     3001 ccatggcagt ctaacttagc ctgcttgtct aaattgactt ctggtaacca cttgggctag
     3061 gacacaggag gaaaggaagg cactgtattt cctatgagct ctctgctcag agcagctgtc
     3121 tgtggtcccg tgggtcctgg attctgtgat ggtatcagca cctggacggc cccagtctag
     3181 attcagcaat gaaaatccac aaagattcag gaaaacttta gctacaaata ccttaaaaat
     3241 gatagattga tgatttcctt aattcctaga atttctttta taaatgcttt gtttacattt
     3301 tagattgtat ctgtccttaa tgtcaaatga tgaatccagt ttgaatcagc tgtcattcca
     3361 agctatcatg ctcacgccat gtcaacagca ccatttgtac taatgctaat aattccatca
     3421 aaatttgcct gtggaacata tattgtcctt aactttaaga tgtcatttcc tgagacagac
     3481 tgtatgaagc tattgtcagc ttctctattt caaatgcaat cagcatatat ctatattgat
     3541 atcagaaaat acttgttttt taaagatata ttgatgtatg ataaagatga tctaatttgt
     3601 gaatgcatat gtgtgcgtgg ttacttttta taatgtgaaa tggataatga gcgtatttaa
     3661 aataaagcag aggttaatga gat
//
