LOCUS       NM_134034               5039 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus protein phosphatase 4 regulatory subunit 3B (Ppp4r3b),
            transcript variant 1, mRNA.
ACCESSION   NM_134034 XM_001479721
VERSION     NM_134034.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Chang,W.H., Choi,S.H., Moon,B.S., Cai,M., Lyu,J., Bai,J., Gao,F.,
            Hajjali,I., Zhao,Z., Campbell,D.B., Weiner,L.P. and Lu,W.
  TITLE     Smek1/2 is a nuclear chaperone and cofactor for cleaved Wnt
            receptor Ryk, regulating cortical neurogenesis
  JOURNAL   Proc Natl Acad Sci U S A 114 (50), E10717-E10725 (2017)
   PUBMED   29180410
  REMARK    GeneRIF: Smek1 and Smek2 (Smek1/2) are key factors in the Ryk
            signaling pathway
REFERENCE   3  (bases 1 to 5039)
  AUTHORS   Moon,B.S., Yun,H.M., Chang,W.H., Steele,B.H., Cai,M., Choi,S.H. and
            Lu,W.
  TITLE     Smek promotes corticogenesis through regulating Mbd3's stability
            and Mbd3/NuRD complex recruitment to genes associated with
            neurogenesis
  JOURNAL   PLoS Biol 15 (5), e2001220 (2017)
   PUBMED   28467410
  REMARK    GeneRIF: Smek/Mbd3 interaction regulates neuronal gene expression
            and neuronal differentiation during cortical development.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5039)
  AUTHORS   Lee,D.H., Acharya,S.S., Kwon,M., Drane,P., Guan,Y., Adelmant,G.,
            Kalev,P., Shah,J., Pellman,D., Marto,J.A. and Chowdhury,D.
  TITLE     Dephosphorylation enables the recruitment of 53BP1 to double-strand
            DNA breaks
  JOURNAL   Mol Cell 54 (3), 512-525 (2014)
   PUBMED   24703952
REFERENCE   5  (bases 1 to 5039)
  AUTHORS   Krol,A.J., Roellig,D., Dequeant,M.L., Tassy,O., Glynn,E.,
            Hattem,G., Mushegian,A., Oates,A.C. and Pourquie,O.
  TITLE     Evolutionary plasticity of segmentation clock networks
  JOURNAL   Development 138 (13), 2783-2792 (2011)
   PUBMED   21652651
REFERENCE   6  (bases 1 to 5039)
  AUTHORS   Lyu,J., Jho,E.H. and Lu,W.
  TITLE     Smek promotes histone deacetylation to suppress transcription of
            Wnt target gene brachyury in pluripotent embryonic stem cells
  JOURNAL   Cell Res 21 (6), 911-921 (2011)
   PUBMED   21423269
  REMARK    GeneRIF: the Smek-containing protein phosphatase 4 complex
            represses transcription of Wnt-responsive development-related genes
            through histone deacetylation, and that this complex is essential
            for embryonic stem cell pluripotency maintenance
REFERENCE   7  (bases 1 to 5039)
  AUTHORS   Thiagarajan,R.D., Georgas,K.M., Rumballe,B.A., Lesieur,E.,
            Chiu,H.S., Taylor,D., Tang,D.T., Grimmond,S.M. and Little,M.H.
  TITLE     Identification of anchor genes during kidney development defines
            ontological relationships, molecular subcompartments and regulatory
            pathways
  JOURNAL   PLoS One 6 (2), e17286 (2011)
   PUBMED   21386911
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5039)
  AUTHORS   Yoon,Y.S., Lee,M.W., Ryu,D., Kim,J.H., Ma,H., Seo,W.Y., Kim,Y.N.,
            Kim,S.S., Lee,C.H., Hunter,T., Choi,C.S., Montminy,M.R. and
            Koo,S.H.
  TITLE     Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic
            subunit (PP4C) is a key regulator of hepatic gluconeogenesis
  JOURNAL   Proc Natl Acad Sci U S A 107 (41), 17704-17709 (2010)
   PUBMED   20876121
REFERENCE   9  (bases 1 to 5039)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 5039)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CD350450.1, AK037253.1, AK133211.1 and BE692419.1.
            
            On May 17, 2018 this sequence version replaced NM_134034.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.256478.1,
                                           SRR1660815.263401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                CD350450.1         70-91
            23-2494             AK037253.1         24-2495
            2495-5016           AK133211.1         680-3201
            5017-5039           BE692419.1         4-26                c
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 16.46 cM"
     gene            1..5039
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /note="protein phosphatase 4 regulatory subunit 3B"
                     /db_xref="GeneID:104570"
                     /db_xref="MGI:MGI:2144474"
     exon            1..472
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     CDS             331..2793
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     3B; SMEK homolog 2, suppressor of mek1"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 3B isoform 1"
                     /protein_id="NP_598795.2"
                     /db_xref="CCDS:CCDS24492.1"
                     /db_xref="GeneID:104570"
                     /db_xref="MGI:MGI:2144474"
                     /translation="MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRA
                     ESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQG
                     KDPSVEVTQDLIDESEEERFEEMPETSHLIDLPACELSKLEEIADLVTSVLSSPIRRE
                     KLALALENEGYIKKLLQLFQACENLENTEGLHHLYEIIRGILFLNKATLFEVMFSDEC
                     IMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDII
                     LPTPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELV
                     NFFKEFCAFSQTLQPQNRDAFFKTLAKLGILPALEIVMGMDDLQVRSAATDIFSYLVE
                     FSPSMVREFVMQEAQQSDDDVLLINVVIEQMICDTDPELGGAVQLMGLLRTLIDPENM
                     LATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKDNMLGSNTTNTICPDNYQT
                     AQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLVLMNSKHTFLALCALRFMRRII
                     GLKDEFYNRYITKGNLFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIV
                     ENFYKALESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEE
                     MWFNEDDDEEGKAVITPVEKSKTEDDFPDSYEKFMETKKAKESEDKENLPKRASSGGF
                     KFTFSHSPSATNGTNSTNSKSVVSQTTPASSNVASSKTTSLATSVTATKGNLVGLVDY
                     PDDEEEDEEEESSPRKRPRLGS"
     exon            473..528
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            529..627
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            628..1251
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1329
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1446
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1563
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1695
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1798
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1840
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1999
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2169
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2309
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2494
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            2495..2697
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..5039
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5017..5022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /note="hexamer: AATAAA"
     polyA_site      5039
                     /gene="Ppp4r3b"
                     /gene_synonym="mKIAA1387; Smek2"
                     /note="major polyA site"
ORIGIN      
        1 agagagtcgg gttacaagat ggcggatctg tagtagtaac cgcggcgggc ggcggcagcg
       61 ggagagcgag cgagctccgg gacgcaaaag gaagggagag tggttgtgcg ccgcggtgaa
      121 gggaggggta gcggagacca cgagcggagg gctctcggtg gctctggtca ccccttctcg
      181 cggctaaacc tttagagcca tggtaaactc gggcctctcc agagtcgctg ccaccaccgc
      241 cgctgccgcc actaccggct ctcaagagtg agggataccg gcgaggtgag cgaggaggtg
      301 gcggctggaa cggtggggcc agaagccacc atgtcggata cacggaggcg agtgaaggtc
      361 tataccctta acgaagaccg gcaatgggac gaccgaggca ccgggcacgt ctcctccact
      421 tacgtagagg agctcaaggg gatgtcgctg ctggttcgtg ccgagtccga cggatcgcta
      481 ctcttagagt caaagataaa tccaaacact gcatatcaga aacagcagga tacattaatt
      541 gtttggtcag aagctgaaaa ctatgatctg gctctaagtt ttcaagaaaa agctggctgt
      601 gatgaaattt gggaaaaaat ttgtcaagtt caaggtaagg acccatcagt agaagtcaca
      661 caggacctca ttgatgaatc tgaagaagaa cgatttgaag aaatgccaga aactagtcat
      721 ctaattgacc tgcccgcatg tgaactcagt aaacttgaag agattgctga cttagttacc
      781 tcagttctct cctcacctat ccgtagggaa aagctggctc tggccttgga aaatgaaggc
      841 tatatcaaaa aactactaca gctattccaa gcttgtgaga acctagaaaa cactgaaggc
      901 ttacaccatc tgtatgaaat tattagagga atcttattcc taaataaggc aacacttttt
      961 gaggtaatgt tttctgatga gtgtatcatg gatgtcgtgg gatgccttga atatgatcct
     1021 gctttggctc agccaaaaag acatagagaa ttcttaacta aaactgcaaa gtttaaggaa
     1081 gttataccaa taacagattc ggaactaagg caaaaaatac atcagactta cagggtacag
     1141 tacattcagg acatcatttt gcctacacca tctgtttttg aagaaaattt cctttctact
     1201 cttacatctt ttattttctt caacaaagtt gaaatagtca gcatgctgca ggaagatgag
     1261 aagtttttgt ctgaagtttt tgcacaatta acagatgaag ctacagatga tgataaacgg
     1321 cgcgagttgg ttaatttctt caaagaattt tgtgcatttt ctcagacatt gcaacctcag
     1381 aacagggatg cttttttcaa aaccttagca aaattgggaa ttcttccagc tcttgaaatt
     1441 gtaatgggca tggatgactt gcaagtcaga tcagctgcta cagatatatt ctcttatctg
     1501 gtggagttta gtccgtccat ggtccgagaa tttgtgatgc aggaagccca gcagagtgat
     1561 gatgatgtac ttcttattaa tgtggtgatt gaacagatga tttgtgatac tgaccctgag
     1621 ctcggaggtg ctgttcagtt aatgggactt cttcgtactc taattgatcc agagaacatg
     1681 ttggctacaa ctaataaaac tgaaaagagt gagttcttaa atttcttcta caaccattgt
     1741 atgcatgttc tcactgctcc acttttgacc aatacgtcag aagacaaatg cgagaaagat
     1801 aatatgcttg gatctaacac aaccaacaca atttgccctg ataattatca aacagcacag
     1861 ctgcttgcct taattttaga gttactcaca ttttgtgtgg aacatcacac ataccacata
     1921 aaaaactaca tcatgaacaa ggacttgcta agaagagtct tggtgttgat gaattcaaag
     1981 cacacttttc tggccttgtg tgctcttcgc tttatgaggc gaataattgg cctgaaagat
     2041 gaattttata atcgttacat caccaaagga aatctttttg agccagttat aaatgccctt
     2101 ttggataatg gaactcggta caatctttta aattcagctg ttattgaatt gtttgaattt
     2161 ataagagtgg aagatatcaa gtctcttact gcacatatag ttgaaaactt ttataaagca
     2221 cttgaatcga ttgaatatgt tcagacattc aaaggattga agactaaata tgaacaagaa
     2281 aaagacagac aaaatcagaa actgaacagt gtaccatcta tattgcgtag taaccgattt
     2341 cgcagagatg caaaagcctt agaagaggat gaagaaatgt ggtttaatga agatgatgat
     2401 gaagaaggaa aggcagtcat aacaccagtt gaaaaatcta agactgaaga tgattttcca
     2461 gatagttatg aaaaatttat ggaaactaaa aaagcaaaag aaagtgaaga caaagaaaac
     2521 cttcccaaaa gggcatcatc tggtggcttc aaatttactt tctcccattc ccccagtgct
     2581 actaatggaa caaacagtac aaacagtaag tctgtagtga gtcagacaac accagcaagt
     2641 tctaatgtgg cctcttccaa aactacaagc ctggctacat cagtgacagc cacaaaggga
     2701 aatttggttg gcttggtaga ctatccagat gatgaagagg aagatgagga agaagaatca
     2761 tcccctagga aaagacctcg cctgggctcc tagaatcctt gctgggggat tcccaccaca
     2821 tggtcttact aactgctgca gctgctcacg cacggagctt gagtctgaat gggaaagctt
     2881 tcatatggaa cttaacacac ggagtagcag agactcttgg ttgccaggta ggaaagtgta
     2941 gttgtgtaag aaccgggctt cccaaaaact taattcctag taattaaagg gtttgccttt
     3001 tgggctgtcc aaaaaagaag aatgataatt agatcctggg tgaaagcttc ccaacaagga
     3061 aaggctcata tttggggtaa gaagggttaa ttgttcccag agtccaacct agaggaaatt
     3121 gtgagacttc agtacccaat aacattgctt gtataatggt gctggccacg ttgttttaat
     3181 cagttgcctt tttttaaaag acatttttta tgtaaagcaa attgaactgt catcagaaat
     3241 gaagttacat tgttgggacc atttctttaa agatttcaca gaagttagcc tttggcattt
     3301 gacaggacat acaccctagg taacaaacag cacataccaa tttctctaca ccaagaactt
     3361 ctaatacatt gaaaaaaaga aaaaagaaag ctgggttcca aatggatagt attcttaaag
     3421 aactaaagtc agcattttta gaggtaacag cagccaagaa atgcagcagc aatgttctga
     3481 aatgctcctc ttccaccaac ctgggagtgg ctagaacagg gggtttccac tatggtcact
     3541 ttctgctttc tcagtaaagt taggtctggg tgttctaaga gctgctggta aatatataaa
     3601 gaccaacaaa gaatggtgct gagttaagtg gcacttaata tctgcaggtc attctagcct
     3661 agaggctact taaatatcac agtcatatgc agcctaggaa gctcttccac agacaagagg
     3721 gtgatgtgaa agctgctttt ttaagagatt taaaaagcac attccatgta tgtaaatgaa
     3781 cttaaaataa ttgaagcaag ctgttaagag cttttatttt ttagaacaag ttaactgtaa
     3841 atattttaat gttagtttgc tcatctatga tctaagatca ttgctgagat gagaaaaatg
     3901 accccaaact acaatttaat gcattgagaa aaaaaaagat ctaaaaatag taattctagc
     3961 tccagtcttt tagatcagct ctataatatg ttatgggtcc atttttcctg gaatagctta
     4021 aaagcagttt cctgtgttgg aaattttgga gttaatttta attttggcta ttgtttggaa
     4081 aagatgggct gtccgtgtag atacgaagta tagtttttcc ataaaacaga tgtttatttt
     4141 gtattaaaaa taccactgta cttgttttac accatttgta tacatgtggt gatattactg
     4201 ctaaactgta aaattcagga attaaaatgt gaccctataa ttccatgatt gaggcttctg
     4261 tttgttttat tgtaaaataa gtgaataagt gaactatttt tgagatatgt ccagtttaca
     4321 ttgtctttta aaggctgata aaatccataa agatatgtta taagccacta ggtcagaaag
     4381 tcttaaatgc aagactcaac cattctttaa atatcactta caagaggtaa accttgagca
     4441 aacctaacag cataaagcca ttctcacctc taaacaaagg tgcacataca tcatgcattc
     4501 atcttgtatc acaagtgaac tgggattctt tcagccaata tcagtaagtt actgtatgag
     4561 taggcattct ttcaaacaag ttttatgaag cctctaccat cttagtgctt agaatctaag
     4621 cagccctgat ctataaactg ttctgttaac tcttttgtta tgttaactaa agtactgtac
     4681 catataaaga cttcctttaa ttttcctttc tgaggaactt caaactttta agacattatt
     4741 atgggaagtg tttcttacaa tggaaatatg aatgttttct taaagtccaa gggcaacagt
     4801 tgtgtaatgc atatgctcac ccacatgatg aaaggatcct tggaaggcac aactaggaag
     4861 attccacagt agctgctgtc ctgaatgacg accctcatgc aggctggggt gcttacagca
     4921 ttatctccac tcagctagtt caagtcagag cacacatttg tatgccttag gtcctaaaaa
     4981 tatctgtaaa catgtaccta ataaaaaact atttttaata aaaagttatg tgaaacaga
//
