LOCUS       NM_211355               4555 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a),
            transcript variant 1, mRNA.
ACCESSION   NM_211355
VERSION     NM_211355.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Duan,R.N., Liu,A., Sun,Y.Q., Xie,Y.F., Wei,S.J., Gao,S., Liu,Y.M.,
            Li,X., Sun,W.J., Li,J.X., Yan,C.Z. and Liu,Q.J.
  TITLE     Loss of Smek1 Induces Tauopathy and Triggers Neurodegeneration by
            Regulating Microtubule Stability
  JOURNAL   Adv Sci (Weinh) 11 (40), e2400584 (2024)
   PUBMED   39206808
  REMARK    GeneRIF: Loss of Smek1 Induces Tauopathy and Triggers
            Neurodegeneration by Regulating Microtubule Stability.
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Wei,S., Song,Y., Li,Z., Liu,A., Xie,Y., Gao,S., Shi,H., Sun,P.,
            Wang,Z., Jin,Y., Sun,W., Li,X., Li,J. and Liu,Q.
  TITLE     SMEK1 ablation promotes glucose uptake and improves obesity-related
            metabolic dysfunction via AMPK signaling pathway
  JOURNAL   Am J Physiol Endocrinol Metab 326 (6), E776-E790 (2024)
   PUBMED   38568153
  REMARK    GeneRIF: SMEK1 ablation promotes glucose uptake and improves
            obesity-related metabolic dysfunction via AMPK signaling pathway.
REFERENCE   3  (bases 1 to 4555)
  AUTHORS   Gao,F., Liu,A., Qi,X., Wang,M., Chen,X., Wei,S., Gao,S., Sun,Y.,
            Sun,P., Li,X., Sun,W., Li,J. and Liu,Q.
  TITLE     Ppp4r3a deficiency leads to depression-like behaviors in mice by
            modulating the synthesis of synaptic proteins
  JOURNAL   Dis Model Mech 15 (6) (2022)
   PUBMED   35314861
  REMARK    GeneRIF: Ppp4r3a deficiency leads to depression-like behaviors in
            mice by modulating the synthesis of synaptic proteins.
REFERENCE   4  (bases 1 to 4555)
  AUTHORS   Duan,R.N., Yang,C.L., Du,T., Liu,A., Wang,A.R., Sun,W.J., Li,X.,
            Li,J.X., Yan,C.Z. and Liu,Q.J.
  TITLE     Smek1 deficiency exacerbates experimental autoimmune
            encephalomyelitis by activating proinflammatory microglia and
            suppressing the IDO1-AhR pathway
  JOURNAL   J Neuroinflammation 18 (1), 145 (2021)
   PUBMED   34183017
  REMARK    GeneRIF: Smek1 deficiency exacerbates experimental autoimmune
            encephalomyelitis by activating proinflammatory microglia and
            suppressing the IDO1-AhR pathway.
            Erratum:[J Neuroinflammation. 2023 Jul 6;20(1):160. doi:
            10.1186/s12974-023-02834-6.. PMID: 37407996]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4555)
  AUTHORS   Chang,W.H., Choi,S.H., Moon,B.S., Cai,M., Lyu,J., Bai,J., Gao,F.,
            Hajjali,I., Zhao,Z., Campbell,D.B., Weiner,L.P. and Lu,W.
  TITLE     Smek1/2 is a nuclear chaperone and cofactor for cleaved Wnt
            receptor Ryk, regulating cortical neurogenesis
  JOURNAL   Proc Natl Acad Sci U S A 114 (50), E10717-E10725 (2017)
   PUBMED   29180410
  REMARK    GeneRIF: Smek1 and Smek2 (Smek1/2) are key factors in the Ryk
            signaling pathway
REFERENCE   6  (bases 1 to 4555)
  AUTHORS   Yoon,Y.S., Lee,M.W., Ryu,D., Kim,J.H., Ma,H., Seo,W.Y., Kim,Y.N.,
            Kim,S.S., Lee,C.H., Hunter,T., Choi,C.S., Montminy,M.R. and
            Koo,S.H.
  TITLE     Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic
            subunit (PP4C) is a key regulator of hepatic gluconeogenesis
  JOURNAL   Proc Natl Acad Sci U S A 107 (41), 17704-17709 (2010)
   PUBMED   20876121
REFERENCE   7  (bases 1 to 4555)
  AUTHORS   Roma,G., Cobellis,G., Claudiani,P., Maione,F., Cruz,P., Tripoli,G.,
            Sardiello,M., Peluso,I. and Stupka,E.
  TITLE     A novel view of the transcriptome revealed from gene trapping in
            mouse embryonic stem cells
  JOURNAL   Genome Res 17 (7), 1051-1060 (2007)
   PUBMED   17540781
REFERENCE   8  (bases 1 to 4555)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 4555)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   10 (bases 1 to 4555)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK053668.1, BC064465.1 and AK054087.1.
            
            On May 15, 2009 this sequence version replaced NM_211355.1.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156272.1, SRR1660821.193956.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-902               AK053668.1         2-903
            903-3831            BC064465.1         601-3529
            3832-4555           AK054087.1         266-989
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 51.17 cM"
     gene            1..4555
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="protein phosphatase 4 regulatory subunit 3A"
                     /db_xref="GeneID:68734"
                     /db_xref="MGI:MGI:1915984"
     exon            1..974
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     CDS             833..3295
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     3A; SMEK homolog 1, suppressor of mek1"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 3A isoform 1"
                     /protein_id="NP_997594.1"
                     /db_xref="CCDS:CCDS26111.1"
                     /db_xref="GeneID:68734"
                     /db_xref="MGI:MGI:1915984"
                     /translation="MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRA
                     ESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQG
                     KDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKL
                     ALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIM
                     DVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLP
                     TPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNF
                     LKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYN
                     PSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLA
                     TANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVE
                     HHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFL
                     FEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTF
                     KGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEA
                     VVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTK
                     TNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDE
                     DDDEDEDKEDTLPVSKKAKFES"
     exon            975..1030
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1129
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1747
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1825
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1942
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2059
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2191
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..2294
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2453
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2623
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2766
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2957
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3184
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..4555
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3899..3904
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="hexamer: ATTAAA"
     polyA_site      3921
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="major polyA site"
     regulatory      4429..4434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="hexamer: AAGAAA"
     polyA_site      4446
                     /gene="Ppp4r3a"
                     /gene_synonym="1110034C04Rik; mKIAA2010; Smek1"
                     /note="major polyA site"
ORIGIN      
        1 agcccgcgct tgcacgacag tacgcgcagg gcagcgcgag ggagtcccgg tccgtcaccg
       61 gcgcgactcg cgtcccgcct cctccgctcc cgccaccccc gcagctccgc gcccgcctcg
      121 gtgcaaggcc tcggccggag cgaagctgcc ggctcccgtc ttgtcgcttt aagtggtggg
      181 gcttgcgggc cgcccctgcc gctactgcct aggtggcccc gcgcgccgct tttgaggggc
      241 gagccaagat ggcggcggct ttggctctgt gaggaagacg gaagaggcgg cggagtaggg
      301 aaggcggctt ctaagaagag ccaggggttc tcgggtggcc agaggcgttc ggagtctccc
      361 gggaagtgct acctcaccgg cttcggatgc tttaagcggg aggagcggag cggggcttcc
      421 tgctgcagcg gctgggccag gtacaaggga ggaaaacatg gcggcggcgg cctgaggagg
      481 cggcggcgcg ggaaagcggc tctgcggagg tcatggtgga ggccggagcg ggtctgtgaa
      541 ccgagccgca cctcgttgga acggcgggcc ggtaggctgc gtagtgctgc gcgttccctg
      601 tcggggctgc tgggcggccg cgctcgctcg gcctggacaa tagcgccggg gagaaggaga
      661 cgagagaagg cggcggcccg gggctcggac ccgggagcgg gccatgcgag cggcggcgca
      721 gctccgcggc ggccagcggt ggcactgagg tcccggagtt ccccgtttgc ggccgcccgc
      781 cttttctgga cactgtccct gggccactcg ggcggggccc ccggccggca cgatgaccga
      841 cacccgacgg cgggtgaagg tctacacgct caacgaggac cggcagtggg acgaccgggg
      901 tactggccat gtgtcctccg gctacgtgga gaggctgaag ggcatgtccc tgctcgtcag
      961 ggcggagagc gacggttctc ttcttctaga gtccaaaata aatcctaaca ctgcatacca
     1021 gaaacaacag gatactctga ttgtgtggtc tgaagcagaa aattatgact tggcccttag
     1081 ctttcaagaa aaagctggat gcgatgaaat ttgggagaaa atatgtcagg ttcaaggaaa
     1141 ggacccttca gtggacatca ctcaggacct ggtagatgaa tctgaagagg agcgatttga
     1201 tgatatgtcc tcaccaggtt tagaattgcc gtcatgtgag ttaagtcgac ttgaggaaat
     1261 tgcagagctt gtggcatcat ctttaccttc ccccctccga cgtgagaaac tcgcactagc
     1321 actggaaaat gagggttata ttaaaaagct tttagagctt tttcatgtgt gtgaggattt
     1381 ggaaaacatt gaaggactgc accacttata tgaaattatt aaagggatct tcctcttgaa
     1441 tcgaactgct ctttttgaag ttatgttctc tgaggaatgt ataatggacg tcattggatg
     1501 cttggaatat gacccaactt tatcacaacc acgtaaacac agggaatttt taacaaaaac
     1561 agccaagttt aaagaagtaa ttcccatatc agatcctgag ctgaaacaaa aaattcatca
     1621 gacatacaga gttcagtata tacaagatat ggttctccct accccttcag tcttcgaaga
     1681 aaacatgtta tcaacacttc actcctttat ctttttcaac aaggtagaaa tcgttggtat
     1741 gttacaggaa gatgaaaaat ttctgacaga tttgtttgca caactaacag atgaagcaac
     1801 agatgaggaa aaaagacaag aattggttaa ctttttaaaa gaattttgtg cattttctca
     1861 aacgctacaa ccccaaaaca gagatgcttt tttcaagact ttgtcaaata tgggcatatt
     1921 accagcttta gaagtcatcc ttggcatgga tgatacacag gtgcgaagtg ctgctacgga
     1981 tatattctca tacttggttg aatataatcc atccatggta cgagagtttg tcatgcagga
     2041 ggcacaacag aatgatgatg atattttgct catcaacctc attatagaac atatgatttg
     2101 tgatacagat cccgagcttg gaggggcagt ccaacttatg ggcctgcttc gaactttagt
     2161 tgacccagag aacatgctag ccactgccaa taaaacagaa aagactgaat ttctgggttt
     2221 cttctacaag cactgtatgc atgttctcac tgctccctta ctagcaaata caacagaaga
     2281 caagcctagt aaagatgatt ttcaaactgc gcagttacta gcacttgtat tggaattgct
     2341 aacattttgt gtggagcacc atacctacca cataaagaac tatattatca acaaggacat
     2401 tcttcgcaga gtgctagttc ttatggcctc caagcatgct ttcttggcat tatgtgccct
     2461 ccgttttaag agaaaaatca ttggattaaa agatgaattt tataaccgct acataatgaa
     2521 aagtttttta tttgaacctg tagtcaaagc atttctcaac aacggatctc gttacaatct
     2581 gatgaactcc gccataatag aaatgtttga atttattaga gtggaagata taaaatcatt
     2641 aactgcccat gtaattgaga attattggaa agcactagaa gatgtagatt atgtacagac
     2701 gtttaaagga ttaaaattga ggtttgaaca gcaaagagaa aggcaagaca accccaaact
     2761 tgatagcatg cggtctatct tgaggaatca tcgatatcga cgagatgcca gaacactaga
     2821 agacgaagaa gagatgtggt tcaacacaga tgaagatgac atggaagatg gagaagctgt
     2881 ggtgtctcca tctgacaaaa cgaagaatga cgatgatatt atggatccaa taagtaaatt
     2941 tatggaaagg aagaaattga aagaaagtga agagaaggag gtgcttctga aaactaatct
     3001 ttctggacga cagagcccaa gtttcaaact ttccttgtcc agtgggacaa agactaacct
     3061 caccagccag tcatccgcaa caagcctgcc tggttctcca gggtcacctg gctccccggg
     3121 ctctccgggc tctcctggat ccgtccctaa gagtacatct cagacggcag ctattaccac
     3181 aaagggagga ctcgtgggtc tggtagatta tcctgatgat gatgaagatg atgatgagga
     3241 tgaagacaag gaagacacac taccagtgtc aaagaaagca aaatttgagt cctaataata
     3301 gcaactgcct gtgatcatca gtacctgttg acaaaactgg ttctttaccc ctccccccta
     3361 caaaatccac atcaaagtgc agtggtctct tgtgaatgac tgacccagat cagcaccaca
     3421 ctggacttct gctcatcaag tgccaattca atggagcagg aggaggggat gccgtgcagt
     3481 taggggaaga cataagcctg tgagctctcc agcttggacc acacattgcc cttttctcag
     3541 ggaaggaaat ggaaataaaa agccaacagg gcaggggttt tgtcagtgga actctggatt
     3601 gactggtcag ttgctacaat cagaacctgc tttcttggac catgtttgag acttagaaga
     3661 atgggtcttt ctgccatagt tcctcactag taaagaatgc cagcagtttc tttgtataaa
     3721 gagacctgcc tttaaaatca tacattctga acattttagt caagctacaa cagatttgga
     3781 aaacctttgt gggggagggg cagaataaag tatctttttt tttttttttt taaatctgtt
     3841 ccctttgccc ttcaaactgc agattttttt tttcaaagtg gggatttgtc cctgcttgat
     3901 taaagattga gtggaattct agatgtggtc atttgtgtca taatttttct ttttgtttca
     3961 atttgtttct gttttttttt tctctccccc tgggtgtatg attagttgtt gaatatatat
     4021 atttgggacc attaaaactt ttttgatgta atatagccta acgtgctggc acctgcttta
     4081 catgtgtaat ttgtgttcta catcacagtt cttaatttgt ttagagtttt atgaaagatg
     4141 gtatagtttt tattgacaaa agcaaagtaa tcttacaact atgtgcatac aaaagcaata
     4201 ctattttgtg actaaatatt ttatattaaa atttgcatca gcagctgtct tgagatttca
     4261 gggaaataga gtggaattta aaacttcaac agttttgtta aacttagaaa catgaaatta
     4321 gtattccaaa gagattcgga aatttcatat cttggagaaa tgacggtaca ttaaatcaaa
     4381 attgaggatg gatgatttaa aagtaatact tgacttttga ataataaaaa gaaaagtgaa
     4441 gagtaagaga aattgtatta attgtatgtt tcttttcttt ctttcttttt ttttaacata
     4501 tgccctttgg aatacaagtt gtaagtggtg gtacctaaat aaacacccaa gaacc
//
