LOCUS       NM_001004025            2985 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus protein phosphatase 3, regulatory subunit B, alpha
            isoform (calcineurin B, type II) (Ppp3r2), mRNA.
ACCESSION   NM_001004025 XM_143782 XM_487795
VERSION     NM_001004025.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Miyata,H., Oura,S., Morohoshi,A., Shimada,K., Mashiko,D., Oyama,Y.,
            Kaneda,Y., Matsumura,T., Abbasi,F. and Ikawa,M.
  TITLE     SPATA33 localizes calcineurin to the mitochondria and regulates
            sperm motility in mice
  JOURNAL   Proc Natl Acad Sci U S A 118 (35) (2021)
   PUBMED   34446558
REFERENCE   3  (bases 1 to 2985)
  AUTHORS   Liu,Y., Zhang,C., Wang,S., Hu,Y., Jing,J., Ye,L., Jing,R. and
            Ding,Z.
  TITLE     Dependence of sperm structural and functional integrity on
            testicular calcineurin isoform PPP3R2 expression
  JOURNAL   J Mol Cell Biol 12 (7), 515-529 (2020)
   PUBMED   31900494
  REMARK    GeneRIF: Dependence of sperm structural and functional integrity on
            testicular calcineurin isoform PPP3R2 expression.
REFERENCE   4  (bases 1 to 2985)
  AUTHORS   Dey,S., Eisa,A., Kline,D., Wagner,F.F., Abeysirigunawardena,S. and
            Vijayaraghavan,S.
  TITLE     Roles of glycogen synthase kinase 3 alpha and calcineurin in
            regulating the ability of sperm to fertilize eggs
  JOURNAL   FASEB J 34 (1), 1247-1269 (2020)
   PUBMED   31914663
REFERENCE   5  (bases 1 to 2985)
  AUTHORS   Zhang,X., Wang,C., Zhao,J., Xu,J., Geng,Y., Dai,L., Huang,Y.,
            Fu,S.C., Dai,K. and Zhang,X.
  TITLE     miR-146a facilitates osteoarthritis by regulating cartilage
            homeostasis via targeting Camk2d and Ppp3r2
  JOURNAL   Cell Death Dis 8 (4), e2734 (2017)
   PUBMED   28383548
  REMARK    GeneRIF: surgical-induced Osteoarthritis mice treated with a
            miR-146a inhibitor significantly alleviated the destruction of
            articular cartilage via targeting Camk2d and Ppp3r2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2985)
  AUTHORS   Yang,S.A. and Klee,C.B.
  TITLE     Low affinity Ca2+-binding sites of calcineurin B mediate
            conformational changes in calcineurin A
  JOURNAL   Biochemistry 39 (51), 16147-16154 (2000)
   PUBMED   11123943
REFERENCE   7  (bases 1 to 2985)
  AUTHORS   Rusnak,F. and Mertz,P.
  TITLE     Calcineurin: form and function
  JOURNAL   Physiol Rev 80 (4), 1483-1521 (2000)
   PUBMED   11015619
  REMARK    Review article
REFERENCE   8  (bases 1 to 2985)
  AUTHORS   Liu,J., Koyano-Nakagawa,N., Amasaki,Y., Saito-Ohara,F., Ikeuchi,T.,
            Imai,S., Takano,T., Arai,N., Yokota,T. and Arai,K.
  TITLE     Calcineurin-dependent nuclear translocation of a murine
            transcription factor NFATx: molecular cloning and functional
            characterization
  JOURNAL   Mol Biol Cell 8 (1), 157-170 (1997)
   PUBMED   9017603
REFERENCE   9  (bases 1 to 2985)
  AUTHORS   Loh,C., Shaw,K.T., Carew,J., Viola,J.P., Luo,C., Perrino,B.A. and
            Rao,A.
  TITLE     Calcineurin binds the transcription factor NFAT1 and reversibly
            regulates its activity
  JOURNAL   J Biol Chem 271 (18), 10884-10891 (1996)
   PUBMED   8631904
REFERENCE   10 (bases 1 to 2985)
  AUTHORS   Ueki,K., Muramatsu,T. and Kincaid,R.L.
  TITLE     Structure and expression of two isoforms of the murine
            calmodulin-dependent protein phosphatase regulatory subunit
            (calcineurin B)
  JOURNAL   Biochem Biophys Res Commun 187 (1), 537-543 (1992)
   PUBMED   1325794
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL732521.18.
            
            On Oct 20, 2007 this sequence version replaced NM_001004025.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029998.1, AK161263.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-544               AL732521.18        134599-135142       c
            545-2985            AL732521.18        131906-134346       c
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 26.75 cM"
     gene            1..2985
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /note="protein phosphatase 3, regulatory subunit B, alpha
                     isoform (calcineurin B, type II)"
                     /db_xref="GeneID:19059"
                     /db_xref="MGI:MGI:107171"
     exon            1..544
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..575
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /EC_number="3.1.3.16"
                     /note="protein phosphatase 2B regulatory subunit 2;
                     calcineurin B subunit; PP2B beta 2"
                     /codon_start=1
                     /product="calcineurin subunit B type 2"
                     /protein_id="NP_001004025.1"
                     /db_xref="CCDS:CCDS18179.1"
                     /db_xref="GeneID:19059"
                     /db_xref="MGI:MGI:107171"
                     /translation="MGNEASYQTELCNHFDQEEIRRLGKSFRKLDLDKSGSLSIEEFM
                     RLPELQQNPLVGRVIDIFDTDGNGEVDFHEFIVGTSQFSVKGDEEQKLRFAFRIYDMD
                     NDGFISNGELFQVLKMMVGNNLKDWQLQQLVDKSILVLDKDGDGRISFEEFSDVVKTM
                     EIHKKLVVFVEHGQEDLKA"
     exon            545..2985
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1294..1299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /note="hexamer: ATTAAA"
     polyA_site      1314
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /note="major polyA site"
     regulatory      1620..1625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
                     /note="hexamer: AATAAA"
     polyA_site      1663
                     /gene="Ppp3r2"
                     /gene_synonym="CaNB2; CnB2"
ORIGIN      
        1 tctcctccct cctttgccag ccctaccgcc tcgccatggg aaatgaggcc agctaccaaa
       61 ctgagctgtg caaccacttc gaccaggaag agataagaag gctgggtaaa agcttcagga
      121 agctggactt ggacaaatcg ggctccctga gcatagaaga gttcatgagg ctgcctgagc
      181 tgcagcagaa tccgctggtg ggccgagtga tcgacatctt cgacacagac ggcaacgggg
      241 aagtggactt ccacgagttc atcgtgggca cctcccagtt cagcgtcaag ggtgatgaag
      301 agcagaagct aaggttcgcc ttcagaatct acgacatgga taatgatggc ttcatctcca
      361 atggggagct cttccaggtg cttaagatga tggtgggaaa caacctcaag gactggcagc
      421 tgcagcagct ggtggacaag agcatcttgg tcctggataa ggatggcgat ggccggatat
      481 cctttgaaga gttcagtgat gtggtcaaga ccatggagat ccacaagaaa ttggtcgtgt
      541 ttgtagaaca cggtcaagaa gacttaaaag cttaaaacta agaaaatgca catctttgcc
      601 tttctttctc aagcttgtgt atgtgggaga agacaacagt tctcatgtgg agtattagga
      661 actccttccc tgtggcattc agacttttaa cagattgtta acttcatgtt gtaacaacta
      721 gcaaaaatat atttatcttt gcttagtgtc ctttaatctc tctcggggca caaagaactc
      781 catagcagga tacacccaga aatccctgag tatgtttgag ggatagaagt ctgaacggga
      841 gattctgtct cagtgcttca gtccaccaga aagtagcttc agtgttcatg tgaaaacagc
      901 taaatctgga ccacatggcc ggcccacccg gtgtggtaca acagcttttc agcatttgag
      961 gttcagaaaa gcaggatttt ggccagatag ggcaacatcc tccccagaaa gagtcctctc
     1021 atccaatacc cagtaggaga gaatgggaga gtccattttc ccaccgggtg gagtgagaag
     1081 atacaggctg acgtaccctt tcgcaggatg cggaagtgcc attgttctgg atgatgagaa
     1141 atacagattc caagcaaaat gaaacttaat gacatcaatg tggtttgctt ttgagaatca
     1201 gatctggtcc ctagcagaga agccaagagc tgtcttatcc caggaaggtt cccctactgt
     1261 aacatccaac tcagagttcc tcatgtctga atcattaaac taataccctt ggaagtgctt
     1321 gaacttgttg attctagaca agaaatgtta cacaactctc tcgccaagtc acttagttct
     1381 tgtcggtggc ctgagctcag cagtgttgga tagtacagca cagaaaggaa caagctctgt
     1441 tctgttttgg agtcagttta tccccatacg tgtgtctagc tctctgaagc cccttagact
     1501 attgccaatt catagagtat aagatgtcct tggaacacct gacgtccatg tgctagcgtc
     1561 catttcacag actgttccct gtaataagct acctgagact ccaaatactg acacagtgaa
     1621 ataaacaatt actgcacata ctcagtcaaa gaacaccact taactactcc tagtttattg
     1681 tcatatttta aagaagctgc caactagtct tggggagtgg gaatccacag atttcattac
     1741 tggcaaggtg cccattaatc ccaaggccaa aactttaaat attatacttt acttcagacc
     1801 cacaaggaat aggaacccct gactctattc ttccttttcc atttgaaagt atctatttgg
     1861 gttgtacaaa taataccgtc cctgtaacag tggagtcaaa cacatttctc aatagttaag
     1921 acagtttgag agccaaatag cccagggtat ggattcaggt agaacagtat agccacacca
     1981 cttatgagtt aggtgaatat gagcacctcg gcctgtgtgt ctttccatct aagtttattc
     2041 tgaggaccca aagagaagaa atgtgtaaag cgtttactgt acagacttcc ctgcctctct
     2101 ctctccctgt atctgtgaga ccagtgtgga aaggaccgtg gggtccttgg atcctcactt
     2161 cctgatatca agctaccact gttctgaaat taaatggaca tctatggaat cctgaccaag
     2221 aaagggaatg aatgatagga gcagacccca agtccctcct caccacgcat gcgcagacca
     2281 caccacagac ttctcaaact gagacataaa gaacacttga aagagagcct ctcatcctag
     2341 cctgacatat ctgactctga gaggaaattt tagtgagaaa ggtgaacacc atccaaacag
     2401 gtctcctgag gacagccatt attagacagc cattagccac aggacagctc tggacatgca
     2461 caccttccaa tagaaaaaaa attgaaatta tgaccaagga tttgaagaat acttagttag
     2521 ctgttcatat ttaataagaa gactcttttg gagtagctgg aataagttac ctgatcaacc
     2581 atatcatagc tacataaaac tttggactga gtagttgttt ctggtttgac aacaaaaatg
     2641 atctggctag atatttgggg aaatgtatta tattgatttc gaagttagaa tgcttttttt
     2701 ttccagctaa ctttcaattt ctcaccaacc aatagttcta tacaacttct tagagtgaca
     2761 gtaagaattg gtgtgggcgg ggcaatcagc tcctaacttt tgtcataact tgcgtgatgt
     2821 catttgatca acactgaggt ggtgccctca tctcagcttt acaagtgaca gagacaatct
     2881 tgtccatgga catgaacctg gtaagaagaa cagaaagatt gaaggccaca aagactgagt
     2941 ttgctttcta ttatattaaa ttcatctcat ttttatagtt cctat
//
