LOCUS       NM_001358933            2831 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus protein phosphatase 1, regulatory subunit 16A
            (Ppp1r16a), transcript variant 1, mRNA.
ACCESSION   NM_001358933 XM_006521467
VERSION     NM_001358933.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Sueyoshi,T., Moore,R., Sugatani,J., Matsumura,Y. and Negishi,M.
  TITLE     PPP1R16A, the membrane subunit of protein phosphatase 1beta,
            signals nuclear translocation of the nuclear receptor constitutive
            active/androstane receptor
  JOURNAL   Mol Pharmacol 73 (4), 1113-1121 (2008)
   PUBMED   18202305
  REMARK    GeneRIF: R16A at the membrane may mediate the PB signal to initiate
            CAR nuclear translocation, through a mechanism including its
            dimerization and inhibition of PP1beta activity.
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Skinner,J.A. and Saltiel,A.R.
  TITLE     Cloning and identification of MYPT3: a prenylatable myosin
            targetting subunit of protein phosphatase 1
  JOURNAL   Biochem J 356 (Pt 1), 257-267 (2001)
   PUBMED   11336659
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156550.5.
            
            On Dec 14, 2017 this sequence version replaced XM_006521467.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1641209.1,
                                           ERR3363660.651116.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AC156550.5         48743-48862
            121-287             AC156550.5         66428-66594
            288-1268            AC156550.5         67272-68252
            1269-1339           AC156550.5         68797-68867
            1340-1485           AC156550.5         68955-69100
            1486-1589           AC156550.5         69937-70040
            1590-1714           AC156550.5         70114-70238
            1715-1840           AC156550.5         70327-70452
            1841-1916           AC156550.5         70539-70614
            1917-2046           AC156550.5         70695-70824
            2047-2209           AC156550.5         70968-71130
            2210-2831           AC156550.5         71398-72019
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 36.25 cM"
     gene            1..2831
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /note="protein phosphatase 1, regulatory subunit 16A"
                     /db_xref="GeneID:73062"
                     /db_xref="MGI:MGI:1920312"
     exon            1..120
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            121..287
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            288..1268
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     CDS             1010..2584
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /note="myosin phosphatase, target subunit 3; mFLJ00392
                     protein; myosin phosphatase targeting subunit 3; protein
                     phosphatase 1, regulatory (inhibitor) subunit 16A"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 16A"
                     /protein_id="NP_001345862.1"
                     /db_xref="CCDS:CCDS27585.1"
                     /db_xref="GeneID:73062"
                     /db_xref="MGI:MGI:1920312"
                     /translation="MAEHLELLAEMPMVGRMSTQERLKHAQKRRAQQVKMWAQAEKEA
                     HSKKGHGERPWKEVAGLRPRKHVLFPPSVALLEAAARNDLEEVRQFLTSGVSPNLANE
                     DGLTALHQCCIDDFQEMAQQLLDAGADVNARDSECWTPLHAAATCGHLHLVELLISRG
                     ADLLAVNSDGNMPYDLCEDAQTLDCLETAMANQGITQEGIEEARAVPELCMLNDLQNR
                     LQAGANLSDPLDHGATLLHIAAANGFSEVATLLLEQGASLSAKDHDGWEPLHAAAYWG
                     QVHLVELLVAHGADLNGKSLVDETPLDVCGDEEVRAKLLELKHKQDALLRAQGRQRSL
                     LRRRTSSAGSRGKVVRRVSLTHRTNLYRKEHAQEAIVWQQPPPTSPEPLEDEDRQTDA
                     ELRLQPPEDDGPEVARPHNGQVGAPPGRHLYSKRLDRSVSYHLSPEENSAPDALVRDK
                     AHHTLAELKRQRAAAKLQRPAPEGPETFEPGLSVDAGTSQPDCGFSTIGDPPLLKLTA
                     PSEEASVEKRPCCLLM"
     exon            1269..1339
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1485
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1589
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1714
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1840
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1916
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2046
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2209
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2831
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2810..2815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /note="hexamer: GATAAA"
     polyA_site      2831
                     /gene="Ppp1r16a"
                     /gene_synonym="2900084E10Rik; Mypt3"
                     /note="major polyA site"
ORIGIN      
        1 cggaagtgtc gcgttgccat ggcgaggatg ggcgcggggc ccgcccccgt ggcgcctgga
       61 gcagcgccgg gctccagggt cggccgggcc gggttcaggg agcggccagg gaggttccag
      121 atcacacaag agtctgtcct caggcacatt ccaagcaggt catgaaggtg ggccactggg
      181 ttaaggacta tgtattgtgg aagacagggc ctcccgggca gtctggagtg gctagaagcc
      241 ttgcttatct tcctgcagca gcagccagag agtgctcagg tgctcaggtg tgtgtgacgg
      301 ccggaagggg aggttcccct gagaaaatat tgacagaact cctgtctcct agctgcctag
      361 agattgtcac ccttggatgc tggaagttgg aatcccagga ctgaccatac cccctgacct
      421 ttgctgccat agcttgttag acctagttgt gctgactgaa aagcctgagg gcagccagca
      481 tggaagagga gcagaggttc ccaggggctg ccaggggctt gctgctgatt ggccctgccc
      541 tccattgtcc taggcagggt gccaggaagc tggaactttg ttatctgggt aattagcttc
      601 agaccctgga ctgaggccgc caggtcttag ggctacttcc cacaggctgt gcaccctgac
      661 cccaagaggg aggcgaggcg ctgtgtacag aagccctgcc agctgaacac ttgaggtgtc
      721 tgtcctacct ggggaacgac tgcaggcatc cccacttaca caccttcctc cccaccccat
      781 gccacactgc cctgcctggc tgggctctgc cccttagggc agctgggcct ggttacagtg
      841 tgggacctcc catctcagcc ttgccaggga cagagggcac caagtcttag aaaggtgatg
      901 cccccaaggg tgcccgtcca gccagctgcc ccacccttca ggcttgtcct ggccccccaa
      961 acccagatcg tggtgccccc aaacaccccg agggcagaca ttcaccacca tggccgagca
     1021 cctggagctg ctggcagaga tgcccatggt aggcaggatg agtacccagg agcgcctgaa
     1081 gcatgcccag aagcgacgtg cccagcaggt aaagatgtgg gcccaggctg agaaggaggc
     1141 tcacagcaag aagggccatg gggagcgacc atggaaggag gtggctggcc tcaggcctcg
     1201 gaagcatgtc ctcttccctc ccagcgttgc ccttctggag gctgctgccc gaaacgactt
     1261 ggaggaagtc cgccagttcc ttaccagtgg ggttagcccc aacctggcca atgaagatgg
     1321 cttgactgca ctgcaccagt gctgcattga cgacttccaa gagatggcac agcagctcct
     1381 ggacgctggg gctgatgtca atgctcgaga cagtgagtgc tggacacctc tgcatgctgc
     1441 agctacctgt ggccatctgc atctggtgga actccttatt tcacgtggtg cagatctcct
     1501 tgcagtcaat tccgatggga atatgcccta cgacctgtgt gaggacgcac agacgctgga
     1561 ttgccttgag actgccatgg ccaaccaggg tatcacccag gagggcattg aggaggcccg
     1621 ggcagtgcca gagctgtgca tgctgaatga cctccagaac cgcctgcaag ctggggccaa
     1681 cctcagtgac cctttggatc atggggctac tctgctgcac atcgccgctg ctaatgggtt
     1741 cagtgaggtg gctaccctgc tgctggagca aggcgccagc ctgagcgcta aggaccatga
     1801 tggctgggag cctctgcatg ctgcggctta ctggggccag gtacacctgg tagaattgct
     1861 tgtggcacat ggggctgatc tgaatggaaa atctctggtg gacgagacac ccctcgatgt
     1921 atgtggtgat gaggaggtga gagccaaact gctggagctc aagcacaagc aagatgcact
     1981 cctgcgggcc cagggccgcc agcgctccct gctgcgccgc cgtacctcta gtgcaggcag
     2041 ccgtgggaag gtggtgagac gagtgagcct gacacatcgc accaacctgt accgcaagga
     2101 gcatgcccaa gaggccattg tgtggcagca gccgccaccc accagtccag aacctctaga
     2161 ggatgaggac aggcagacag acgctgagct ccggctgcag cccccggagg acgatggccc
     2221 tgaggtggcc agaccacaca atggccaagt aggggccccc cccgggagac acctgtactc
     2281 caagcgtcta gatcggagtg tctcctatca tctgagtcct gaggagaaca gtgcccctga
     2341 tgccctcgtc cgggacaagg cccaccacac actggcagaa ctgaaacgcc agcgagcggc
     2401 tgcaaagctt cagcgacctg cccctgaagg gcctgagact tttgagcctg gcctgtctgt
     2461 tgatgctggg acctcccagc ctgactgtgg cttcagcaca attggggacc cgcccctgct
     2521 caagctcact gctccctcgg aggaggcgtc tgtggagaag aggccgtgtt gcttgctcat
     2581 gtgaaatgtg attcctcagt atggcagggt tcaaccccat gcccagccgg aggcttcctc
     2641 acgatctctg gcctggtgac ccacttagca catacccctg tgctgtgggg gagccggcac
     2701 agagccccat ccccctccca gcccaggaca ctggccacct tgctcaggga tggactatgc
     2761 agagctctac ctatgggcct caggccacag tctgtttatt gtgactcttg ataaaggctg
     2821 tttgccacgg a
//
