LOCUS       NM_133819               5588 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus protein phosphatase 1, regulatory subunit 15B
            (Ppp1r15b), mRNA.
ACCESSION   NM_133819
VERSION     NM_133819.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Kumar,A., Karuppagounder,S.S., Chen,Y., Corona,C., Kawaguchi,R.,
            Cheng,Y., Balkaya,M., Sagdullaev,B.T., Wen,Z., Stuart,C., Cho,S.,
            Ming,G.L., Tuvikene,J., Timmusk,T., Geschwind,D.H. and Ratan,R.R.
  TITLE     2-Deoxyglucose drives plasticity via an adaptive ER stress-ATF4
            pathway and elicits stroke recovery and Alzheimer's resilience
  JOURNAL   Neuron 111 (18), 2831-2846 (2023)
   PUBMED   37453419
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Park,E.J., Kim,H.S., Lee,D.H., Kim,S.M., Yoon,J.S., Lee,J.M.,
            Im,S.J., Lee,H., Lee,M.W. and Lee,C.W.
  TITLE     Ssu72 phosphatase is essential for thermogenic adaptation by
            regulating cytosolic translation
  JOURNAL   Nat Commun 14 (1), 1097 (2023)
   PUBMED   36841836
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5588)
  AUTHORS   Xu,X., Krumm,C., So,J.S., Bare,C.J., Holman,C., Gromada,J.,
            Cohen,D.E. and Lee,A.H.
  TITLE     Preemptive Activation of the Integrated Stress Response Protects
            Mice From Diet-Induced Obesity and Insulin Resistance by Fibroblast
            Growth Factor 21 Induction
  JOURNAL   Hepatology 68 (6), 2167-2181 (2018)
   PUBMED   29698569
  REMARK    GeneRIF: CReP deletion activates a linear signaling pathway
            involving eIF2alpha phosphorylation, ATF4 translation, and FGF21
            induction, which promotes increased energy expenditure, and
            subsequently improved obesity-related metabolic parameters.
REFERENCE   4  (bases 1 to 5588)
  AUTHORS   Reid,D.W., Tay,A.S., Sundaram,J.R., Lee,I.C., Chen,Q., George,S.E.,
            Nicchitta,C.V. and Shenolikar,S.
  TITLE     Complementary Roles of GADD34- and CReP-Containing Eukaryotic
            Initiation Factor 2alpha Phosphatases during the Unfolded Protein
            Response
  JOURNAL   Mol Cell Biol 36 (13), 1868-1880 (2016)
   PUBMED   27161320
  REMARK    GeneRIF: Results highlighted the essential roles played by GADD34
            and CReP in regulating mRNA translation during unstressed
            conditions and following endoplasmic reticulum stress.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5588)
  AUTHORS   So,J.S., Cho,S., Min,S.H., Kimball,S.R. and Lee,A.H.
  TITLE     IRE1alpha-Dependent Decay of CReP/Ppp1r15b mRNA Increases
            Eukaryotic Initiation Factor 2alpha Phosphorylation and Suppresses
            Protein Synthesis
  JOURNAL   Mol Cell Biol 35 (16), 2761-2770 (2015)
   PUBMED   26031337
  REMARK    GeneRIF: CReP expression was markedly suppressed in XBP1-deficient
            mice livers due to hyperactivated Ire1alpha.
REFERENCE   6  (bases 1 to 5588)
  AUTHORS   Chambers,J.E., Dalton,L.E., Clarke,H.J., Malzer,E., Dominicus,C.S.,
            Patel,V., Moorhead,G., Ron,D. and Marciniak,S.J.
  TITLE     Actin dynamics tune the integrated stress response by regulating
            eukaryotic initiation factor 2alpha dephosphorylation
  JOURNAL   Elife 4, e04872 (2015)
   PUBMED   25774599
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5588)
  AUTHORS   Harding,H.P., Zhang,Y., Scheuner,D., Chen,J.J., Kaufman,R.J. and
            Ron,D.
  TITLE     Ppp1r15 gene knockout reveals an essential role for translation
            initiation factor 2 alpha (eIF2alpha) dephosphorylation in
            mammalian development
  JOURNAL   Proc Natl Acad Sci U S A 106 (6), 1832-1837 (2009)
   PUBMED   19181853
  REMARK    GeneRIF: Ppp1r15a mutant mice are superficially indistinguishable
            from wild type; Ppp1r15b(-/-) mouse embryos survive gestation but
            exhibit severe growth retardation and impaired erythropoiesis, and
            loss of both Ppp1r15 genes leads to early embryonic lethality
REFERENCE   8  (bases 1 to 5588)
  AUTHORS   Cinelli,P., Casanova,E.A., Uhlig,S., Lochmatter,P., Matsuda,T.,
            Yokota,T., Rulicke,T., Ledermann,B. and Burki,K.
  TITLE     Expression profiling in transgenic FVB/N embryonic stem cells
            overexpressing STAT3
  JOURNAL   BMC Dev Biol 8, 57 (2008)
   PUBMED   18500982
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 5588)
  AUTHORS   Latreille,M. and Larose,L.
  TITLE     Nck in a complex containing the catalytic subunit of protein
            phosphatase 1 regulates eukaryotic initiation factor 2alpha
            signaling and cell survival to endoplasmic reticulum stress
  JOURNAL   J Biol Chem 281 (36), 26633-26644 (2006)
   PUBMED   16835242
REFERENCE   10 (bases 1 to 5588)
  AUTHORS   Jousse,C., Oyadomari,S., Novoa,I., Lu,P., Zhang,Y., Harding,H.P.
            and Ron,D.
  TITLE     Inhibition of a constitutive translation initiation factor 2alpha
            phosphatase, CReP, promotes survival of stressed cells
  JOURNAL   J Cell Biol 163 (4), 767-775 (2003)
   PUBMED   14638860
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC107842.13 and AK146089.1.
            
            On Aug 18, 2009 this sequence version replaced NM_133819.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058078.1, SRR9219381.35655.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-263               AC107842.13        150785-151047
            264-3385            AK146089.1         2-3123
            3386-5588           AC107842.13        157217-159419
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.8 cM"
     gene            1..5588
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /note="protein phosphatase 1, regulatory subunit 15B"
                     /db_xref="GeneID:108954"
                     /db_xref="MGI:MGI:2444211"
     exon            1..2450
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /inference="alignment:Splign:2.1.0"
     CDS             582..2675
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /note="constitutive repressor of eIF2alpha
                     phosphorylation; protein phosphatase 1, regulatory
                     (inhibitor) subunit 15b"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 15B"
                     /protein_id="NP_598580.1"
                     /db_xref="CCDS:CCDS15291.1"
                     /db_xref="GeneID:108954"
                     /db_xref="MGI:MGI:2444211"
                     /translation="METGTHRARKRPGPRLGSWFRLPFLRRSHACSSEFPPPSSRQNP
                     GNSALPERRTRYWTKLLSQLLALLPSLFQKLLLWSQLFGGLIPTRWLDFAASYSALRA
                     LRGREESAAPTVQKSLSSLRLDSSEDLVVSSLDWLEEGLQWQCSSSDLELKLKAQERA
                     LDSAAPTFLLEQQLWGVELLPSSLQAGLVSHRELDSSSSGPLSVQSLGNFKVVSYLLN
                     PSYLDYLPQLGLRCQSSAGGGQFVGFRTLTPESCYLSEDGCHPQPLRAEMSATAWRRC
                     PPLSTEGLPEIHHLRMKRLEFLQANKGQELPTPDQDNGYHSLEEEHNLLRMDPQHCTD
                     NPAQAVSPAADRPEPTEKKPELVIQEVSQSPQGSSLFCELPVEKECEEDHTNATDLSD
                     RGESLPVSTRPVCSNKLIDYILGGAPSDLEASSDSESEDWGEEPEDDGFDSDGSLSES
                     DVEQDSEGLHLWNSFHSVDPYNPQNFTATIQTAARIAPRDPSDSGTSWSGSCGVGSCQ
                     EGPLPETPDHSSGEEDDWEPSADEAENLKLWNSFCHSEDPYNLLNFKAPFQPSGKNWK
                     GRQDSKASSEATVAFSGHHTLLSCKAQLLESQEDNCPGCGLGEALAGERYTHIKRKKV
                     TFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEVAIGYCLAFEHREKMFNRL
                     RIESKDLLLYSNVKK"
     exon            2451..5588
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5566..5571
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /note="hexamer: ATTAAA"
     polyA_site      5571
                     /gene="Ppp1r15b"
                     /gene_synonym="1810033K10Rik; C530022L24Rik; CReP"
                     /note="major polyA site"
ORIGIN      
        1 acagacgcgg ccaatcagag gccacgcacg agggtgaggc aagtccgcct ctcgggaggc
       61 aagggtgggg gttcggcgaa tgacgtgagg cgccgctggg cggtagggag tagacgccct
      121 cggtcctccg tctcgccctg cagcttccgg gtgtgcggct gcggccattt tgagcttcgc
      181 ttctttgcgc cctcgcctgc cacccagcca ccctttccgc cttggcgttt cgcgcctccg
      241 tgcgggccac cggaaacgcc gccgtcgtct ccgtcgccgc cgcgcgaggg agggtcttct
      301 ctatggtgga gcgatctcac acggcctagg acgtctcctt ccctagccgg gatggaccta
      361 accgcggtcg ccaccgcttg cgcgggcctc tgggccgtcc ggtgcagcac tcgttgcgga
      421 agccgccgct ctctgggcct cctctgccgg cgcgggaatc ggactgcagt acccactccg
      481 tggctgggca aggcggagac tgtgtagacc tcggatccag cctgcgctga cgccgctgag
      541 ctctgtcctc ctcctgtctg agaagccgcc aaggaaagga gatggagaca ggaacgcaca
      601 gggcccggaa gcggcctggc cctcggctgg gctcctggtt ccggctgccc ttccttcggc
      661 gatcgcacgc ctgctcttcg gagttcccgc cgccttcctc tcgacaaaat cccgggaact
      721 ccgctctgcc cgagcgtcgg accaggtact ggaccaaatt gctttctcag ctccttgccc
      781 tgctccctag cctattccag aagctgctgc tttggagcca gcttttcggg ggcctgattc
      841 ctaccagatg gctagatttt gccgcaagtt acagcgccct gagagctttg agaggacggg
      901 aggaatctgc cgctcccacg gtgcagaagt ctctgagttc actgcggctg gactcttcgg
      961 aagacttggt tgtcagttct cttgattggc tagaggaggg actccagtgg cagtgctcgt
     1021 cctcagacct ggagttaaaa ctcaaggccc aggaaagagc tttagactct gcagcgccca
     1081 ctttcctcct ggagcagcag ctgtggggag tggagttgct gcccagtagc cttcaagctg
     1141 gtctagtctc ccaccgagaa cttgactctt catcctctgg gcctctgagc gttcagagct
     1201 taggtaattt caaggtagtt tcctatctcc tgaacccttc ctacctggac taccttcccc
     1261 agttagggct gcgctgtcag agcagcgctg gaggtggcca gtttgtgggt ttccgaacac
     1321 taaccccaga gagctgctat ctttctgaag atggttgtca ccctcagccg ttgcgggcag
     1381 agatgtcggc aaccgcctgg agaaggtgtc cgcctctctc tacagaaggc ctgccggaaa
     1441 tccaccacct tcgtatgaaa cggctagaat tcctccaggc taacaaaggg caagagttac
     1501 ccacccctga ccaagataat ggctatcata gcctggagga ggaacataac cttctccgga
     1561 tggacccaca acattgcaca gataacccag cacaggcggt gtcccctgct gcagacaggc
     1621 cggagcccac tgagaaaaaa ccagaattgg tgattcaaga agtttcacag agcccccagg
     1681 gaagcagtct gttttgtgaa ttacccgtgg aaaaagaatg tgaagaggac cacactaatg
     1741 caactgacct ctcagataga ggagagagcc ttcctgtttc taccagacca gtttgtagca
     1801 acaaactgat agattatatt ttgggaggcg cccccagtga cttggaagcc agctctgatt
     1861 ctgaaagtga ggattggggc gaggaacctg aggacgatgg ctttgatagc gatggctccc
     1921 tgtctgaatc agacgtggaa caggactcgg aaggccttca cctttggaac tctttccaca
     1981 gtgtagatcc ttacaacccc caaaacttta cagccacgat tcagacggct gccagaattg
     2041 cccccagaga cccatcagat tcagggacat cctggtctgg cagctgtggt gtagggagct
     2101 gtcaggaggg accccttccg gagacccccg accatagttc cggggaggaa gatgactggg
     2161 aaccgagtgc agatgaagca gagaatctta aattgtggaa ctctttctgt cattctgagg
     2221 acccctacaa ccttttaaat tttaaggctc cttttcaacc gtcagggaag aattggaaag
     2281 gccgtcagga ctcaaaggcc tcttctgagg ctacagtggc cttctctggc catcatacct
     2341 tactttcttg taaggcccag ctgttagaga gccaagaaga taattgtcca ggctgtgggc
     2401 tgggtgaggc tcttgctgga gaaagataca cccatatcaa gagaaaaaag gtaaccttcc
     2461 tggaagaagt tactgagtat tatataagtg gtgatgagga tcgcaaagga ccatgggaag
     2521 aatttgcaag ggatggatgc aggttccaga aacgaattca agaaacagaa gttgccattg
     2581 gctactgctt ggcctttgag cacagagaaa aaatgtttaa tagactgagg atcgagtcaa
     2641 aggacttact gttgtacagc aatgttaaga agtgaacagc ctgcaacccg tgcccactct
     2701 gtctcttact tgagagtttc ccttaaaaac aaacactggc agctgtcctt ggacatgttt
     2761 ttaaagaaac aacttgtatc tagagatgca gtttgattat ttttgggtaa tgtgtctcat
     2821 tagaaacacc aactccgata atgaagaatc tcttatctgt aatcctctct tttcctattt
     2881 agttggatgt gggttttgtc tttttgagag ggtctcactg cataattctg tttggcctag
     2941 gtctatgtat gtagaccagg ttggccttga agttggtatt tccttgcctc tgtttcctgt
     3001 gccaccatgc ccagctggaa gtgtttttaa atatcctctg cttacctggg gtgagagttt
     3061 aattttgcac tttcaaaacg tttcttctgg aggcaggggc tggtctggtt tacatacagg
     3121 cttcaggcca gctagggcta tgtggtgaga cctagtctta gaggacaaac agaacaaaac
     3181 agtcaggtta ctgtggaaac tgaggcagga ggataggaag gtccaggtat gcctggaact
     3241 cagtgaactc tgaactcaag gccagcatgg ggagtttagc aagacctttt tacactcaga
     3301 atggaaaagg aagctgggga tatagctcgg tagcagaggc ccctgcctac atgtgcaagg
     3361 tcctgggttc agtccccagt actgcaaata gaaagaaaaa acattgtctt ggataactat
     3421 aaggtttaag cctcatagtc agttctaact caaattatgc atgcattgag ttcttgtgtg
     3481 ctttttctgt tctaaaatta atgggcttta tgggttttgt ttttgtatgt ttttatgtct
     3541 gttatatttt gaggtagggg attgaggcag ggtctcactg tggccttgag ctcatggcag
     3601 ttttcctgct tcaactgagt gttttggttt gtttgtgttt gtgttttttt actttcatag
     3661 atttgactta atgaaggcaa aaacctgtta tcaacctaaa agacattgat gtgtcacttc
     3721 agtggtggat tttctccctc tttttttttt tcccccagag ctgaggacca aacccagggc
     3781 tttgcacttg ctaggcaagc gctctaccac tgagctaaat ccccaaacca cccccccccc
     3841 ttttcttttt ttaaagacat ggtcttatat agtctaggct ggccttaaac tcattactta
     3901 gccaaggatg gctttgaaag atcctcctgt cctcttgttt ggcttgcaca tggcatgtgc
     3961 catacatcca gatttttctc tgtatctagg tctttttaag atatttccaa ggactgccca
     4021 caaatccaca gtgcagtact ttcttgactg ggaaagcggg gttggtgtgc ttttcaaagg
     4081 cacacaccat tatgcccagc tggtctgcca aacttaacat atttggtttc tgtgcaaact
     4141 gtccatttgg aaggtttctg tgtgttgtag tttcagttga atgtggctct ctgtagcttt
     4201 caagaatggg tagaaatcat aaagcactct taagtaatca ttgcattgta gacatttttt
     4261 tttttaacta gggggtattt gggagacatg tcagatattc acttttgttt tatgtgtctt
     4321 aaaaccagtg ttacttacac cctgcctcag cactggcact tttcaaacct gtcttggaga
     4381 cactgtaaac ttggatggtg cagttctggg ttttcatgtg taaaatgtca actacaaagg
     4441 tcaatatcca ggtttgttgt gttctacctt atgtagagag aagcaaagca gaaagggcag
     4501 atagagcagc cagaaagtgc tagtgtcccc ccccaaagcg tgcttctaga tagtgtgtgg
     4561 atcaggtgtg cttggtttgt tcagttagcg tcacctgttg tgcatggttg aggtaatgtg
     4621 ttaccagttc tttagtggtt acatgcacaa aagagaggac ctctgagtcg ggtgtgggat
     4681 gagctttcca gacctggcag ggtaaactac ctcagtttat aatctccctg gttatttccg
     4741 tttgatgtga tctaaggtct gcctcagtgg tgatgatgtt catccacaca caaggttagt
     4801 aagagtgcac gaccagaaac gttggtctta tttttgagaa cccccatttc tgtgtatttt
     4861 atgcacctgc ctttagtgaa ctccagagtg cattaaagag tctggtttag tgccgtggga
     4921 atgggctagt ttagaagcta tgtttggaaa gcaggcaagt tgactttagg aagaaaagct
     4981 gtgacagtgt gtagacattt cttttaaacc ggactgcagc ttaacaacac ttgatttcag
     5041 atgattaggt ttttgtttct gagacccagc acctgtatat ttaaaaattg ttccagatta
     5101 caccttcact atcaaatgag taaatgactc atgcctgcag acatgtcctg atggtggcaa
     5161 gaacagaagg atctttgact gaaggagaaa aactgtcatt gtcatcccag cccccaggaa
     5221 agaacacctc caaggcaggc aggcaggcag gcaggcatgg tggttctagt tgaatacaca
     5281 ttcaagtctt gcagtggtgc tttagatctg tgtagcatgt gaggctctgt acaggtgggg
     5341 gccacacttc tgagggctga aatgtggcaa ccctttatct aacttgaaat caaaaccgtc
     5401 aaattttatt ttttataatt taagaaagag ttggggaatg acattttttg agttggcctt
     5461 ttcagctcag tcattttacg tgtaacgtgg agatttgata gctcagatta tatttgtata
     5521 taattattaa ctaatctgta aattgtaata aatatatttg caattattaa atgctatgtg
     5581 actttatg
//
