LOCUS       NM_008654               2345 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus protein phosphatase 1, regulatory subunit 15A
            (Ppp1r15a), mRNA.
ACCESSION   NM_008654
VERSION     NM_008654.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Bouchla,A., Sotiropoulou,C.D., Esteb,C., Loupis,T.,
            Papageorgiou,S.G., Deliconstantinos,G.G., Pagoni,M.,
            Hatzimichael,E., Dellatola,M., Kalomoiri,S., Apostolidou,E.,
            Kontos,C.K., Thomopoulos,T.P., Karantanos,T. and Pappa,V.
  TITLE     Silencing of the DNA damage repair regulator PPP1R15A sensitizes
            acute myeloid leukemia cells to chemotherapy
  JOURNAL   Ann Hematol 103 (8), 2853-2863 (2024)
   PUBMED   38842564
  REMARK    GeneRIF: Silencing of the DNA damage repair regulator PPP1R15A
            sensitizes acute myeloid leukemia cells to chemotherapy.
REFERENCE   3  (bases 1 to 2345)
  AUTHORS   Schraps,N., Tirre,M., Pyschny,S., Reis,A., Schlierbach,H.,
            Seidl,M., Kehl,H.G., Schanzer,A., Heger,J., Jux,C. and
            Drenckhahn,J.D.
  TITLE     Cardiomyocyte maturation alters molecular stress response
            capacities and determines cell survival upon mitochondrial
            dysfunction
  JOURNAL   Free Radic Biol Med 213, 248-265 (2024)
   PUBMED   38266827
REFERENCE   4  (bases 1 to 2345)
  AUTHORS   Oliveira,M.M., Mohamed,M., Elder,M.K., Banegas-Morales,K.,
            Mamcarz,M., Lu,E.H., Golhan,E.A.N., Navrange,N., Chatterjee,S.,
            Abel,T. and Klann,E.
  TITLE     The integrated stress response effector GADD34 is repurposed by
            neurons to promote stimulus-induced translation
  JOURNAL   Cell Rep 43 (2), 113670 (2024)
   PUBMED   38219147
REFERENCE   5  (bases 1 to 2345)
  AUTHORS   Hayakawa-Ogura,M., Tana, Nakagawa,T. and Itoh,M.
  TITLE     GADD34 suppresses eIF2alpha phosphorylation and improves cognitive
            function in Alzheimer's disease-model mice
  JOURNAL   Biochem Biophys Res Commun 654, 112-119 (2023)
   PUBMED   36907138
  REMARK    GeneRIF: GADD34 suppresses eIF2alpha phosphorylation and improves
            cognitive function in Alzheimer's disease-model mice.
REFERENCE   6  (bases 1 to 2345)
  AUTHORS   Lee,Y.Y., Cevallos,R.C. and Jan,E.
  TITLE     An upstream open reading frame regulates translation of GADD34
            during cellular stresses that induce eIF2alpha phosphorylation
  JOURNAL   J Biol Chem 284 (11), 6661-6673 (2009)
   PUBMED   19131336
  REMARK    GeneRIF: GADD34 translation is regulated by a unique 5'UTR uORF
            mechanism to ensure proper GADD34 expression during eIF2alpha
            phosphorylation
REFERENCE   7  (bases 1 to 2345)
  AUTHORS   Cheng,G., Gross,M., Brett,M.E. and He,B.
  TITLE     AlaArg motif in the carboxyl terminus of the gamma(1)34.5 protein
            of herpes simplex virus type 1 is required for the formation of a
            high-molecular-weight complex that dephosphorylates eIF-2alpha
  JOURNAL   J Virol 75 (8), 3666-3674 (2001)
   PUBMED   11264356
REFERENCE   8  (bases 1 to 2345)
  AUTHORS   Weiss,R.S., Enoch,T. and Leder,P.
  TITLE     Inactivation of mouse Hus1 results in genomic instability and
            impaired responses to genotoxic stress
  JOURNAL   Genes Dev 14 (15), 1886-1898 (2000)
   PUBMED   10921903
REFERENCE   9  (bases 1 to 2345)
  AUTHORS   Hollander,M.C., Zhan,Q., Bae,I. and Fornace,A.J. Jr.
  TITLE     Mammalian GADD34, an apoptosis- and DNA damage-inducible gene
  JOURNAL   J Biol Chem 272 (21), 13731-13737 (1997)
   PUBMED   9153226
REFERENCE   10 (bases 1 to 2345)
  AUTHORS   Lord,K.A., Hoffman-Liebermann,B. and Liebermann,D.A.
  TITLE     Sequence of MyD116 cDNA: a novel myeloid differentiation primary
            response gene induced by IL6
  JOURNAL   Nucleic Acids Res 18 (9), 2823 (1990)
   PUBMED   2339071
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151602.4, AK079339.1, BC138486.1 and AW493042.1.
            
            On Sep 17, 2009 this sequence version replaced NM_008654.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X51829.1, BC138486.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            regulatory uORF        :: PMID: 19131336
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC151602.4         64196-64239
            45-135              AK079339.1         2-92
            136-2317            BC138486.1         1-2182
            2318-2345           AW493042.1         1-28                c
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.34 cM"
     gene            1..2345
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /note="protein phosphatase 1, regulatory subunit 15A"
                     /db_xref="GeneID:17872"
                     /db_xref="MGI:MGI:1927072"
     exon            1..274
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      180..260
                     /regulatory_class="uORF"
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19131336]"
                     /note="this uORF is predicted to encode a 26 aa peptide
                     (PMID:19131336)"
     exon            275..1927
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..2257
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /note="growth arrest and DNA damage-inducible protein
                     GADD34; myeloid differentiation primary response protein
                     MyD116; myeloid differentiation primary response gene 116;
                     growth arrest and DNA-damage-inducible 34; protein
                     phosphatase 1, regulatory (inhibitor) subunit 15A"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 15A"
                     /protein_id="NP_032680.1"
                     /db_xref="CCDS:CCDS21249.1"
                     /db_xref="GeneID:17872"
                     /db_xref="MGI:MGI:1927072"
                     /translation="MAPSPRPQHVLHWRDAHNFYLLSPLMGLLSRAWSRLRGPEVPEA
                     WLAKTVTGADQIEAAALLTPTPVSGNLLPHGETEESGSPEQSQAAQRLCLVEAESSPP
                     ETWGLSNVDEYNAKPGQDDLREKEMERTAGKATLQPAGLQGADKRLGEVVAREEGVAE
                     PAYPTSQLEGGPAENEEDGETVKTYQASAASIAPGYKPSTPVPFLGEAEHQATEEKGT
                     ENKADPSNSPSSGSHSRAWEYYSREKPKQEGEAKVEAHRAGQGHPCRNAEAEEGGPET
                     TFVCTGNAFLKAWVYRPGEDTEEEDNSDSDSAEEDTAQTGATPHTSAFLKAWVYRPGE
                     DTEEEDSDSDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEENSDLDSAEEDTAQTG
                     ATPHTSAFLKAWVYRPGEDTEEENSDLDSAEEDTAQTGATPHTSPFLKAWVYRPGEDT
                     EDDTEEEEDSENVAPGDSETADSSQSPCLQPQRCLPGEKTKGRGEEPPLFQVAFYLPG
                     EKPESPWAAPKLPLRLQRRLRLFKAPTRDQDPEIPLKARKVHFAEKVTVHFLAVWAGP
                     AQAARRGPWEQFARDRSRFARRIAQAEEKLGPYLTPDSRARAWARLRNPSLPQSEPRS
                     SSEATPLTQDVTTPSPLPSETPSPSLYLGGRRG"
     exon            1928..2333
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2305..2310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /note="hexamer: AATAAA"
     polyA_site      2333
                     /gene="Ppp1r15a"
                     /gene_synonym="9630030H21; Gadd34; Myd116"
                     /note="major polyA site"
ORIGIN      
        1 agcgccgcgt cagggtataa aagccgcgtg gacgatgttg gcgcagattg agtcagctct
       61 gagtttgtgg aagattacat gcgatatccc gcgcgacccc gcatcccttt gccggccggg
      121 acagcctttg ctacagcctg tgaaacattg cgtccccgag ccccacgcct gagggcgaca
      181 tgaacccgct ggcttcgcga gcagtccgga cccacgatcg cttttggcaa ccagaaccgg
      241 cgcttcagcc cccggggtga cgtgcagccc gccgcccaga cacatggccc cgagcccaag
      301 accccagcat gtcctgcact ggagggacgc ccacaacttc tatctcctgt ccccactgat
      361 gggcttgctc agtcgggcct ggagccgcct gaggggccca gaagtcccag aggcatggct
      421 ggcaaaaaca gtaacaggag cagatcagat agaagctgcg gctctgctga cacctacccc
      481 tgtctctggt aacctcctcc ctcatgggga gactgaagaa agtggatctc ctgaacagag
      541 tcaagcagcc cagaggctct gccttgtgga agctgaaagt tcccctcctg aaacttgggg
      601 actttcaaat gttgatgagt acaatgcaaa gccaggacaa gatgacctta gagagaagga
      661 aatggaacgc acagctggca aggccacact acagcccgct ggcctgcaag gggctgataa
      721 gaggcttggg gaggtggtgg ctagagaaga gggagtggct gagcccgctt atcccacatc
      781 acagctggag ggtggtccag ctgagaatga agaggatgga gaaacagtga agacttacca
      841 agcttctgct gcttccatag ctccgggata caaacccagc acccctgtgc ctttcttggg
      901 ggaggcagaa catcaagcca cggaagaaaa aggaacagaa aacaaggctg acccctccaa
      961 ctctccttct tcaggctccc actccagagc ctgggagtac tactctagag agaagcctaa
     1021 gcaggaggga gaagccaagg tagaggcaca cagggcaggg cagggtcacc cttgtcggaa
     1081 tgctgaggct gaggaaggag gacctgagac aacttttgtc tgtactggaa atgccttcct
     1141 gaaggcctgg gtgtatcgcc caggagagga cacagaggaa gaagacaaca gcgattcgga
     1201 ttcagctgag gaagacacag ctcagaccgg tgccaccccc catacaagtg ccttcctgaa
     1261 ggcctgggtg tatcgcccag gagaggacac agaggaagaa gacagcgatt cggattcagc
     1321 tgaggaagac acagctcaga ccggtgccac cccccataca agtgccttcc tgaaggcctg
     1381 ggtgtatcgc ccaggagagg acacagagga agaaaacagc gatttggatt cagctgagga
     1441 agacacagct cagaccggtg ccacccccca tacaagtgcc ttcctgaagg cctgggtgta
     1501 tcgcccagga gaggacacag aggaagaaaa cagcgatttg gattcagctg aggaagacac
     1561 agctcagacc ggtgccaccc cacatacaag tcccttcctg aaggcctggg tgtatcgccc
     1621 aggagaggac acagaagatg acacagaaga ggaagaggac agtgagaatg tggccccagg
     1681 tgactcagaa acagctgact caagccagag tccctgcctt cagccccagc gttgtctacc
     1741 aggagagaag accaagggac gtggggaaga gccccctctc ttccaggtgg ccttctattt
     1801 acccggagag aagccagaat caccttgggc tgcacctaag ctgccccttc gactgcagag
     1861 gcggctcaga ttgttcaaag cccccacccg ggatcaggac cccgagattc ctctaaaagc
     1921 tcggaaggta cacttcgctg agaaagtcac agtccatttc cttgctgtct gggcaggacc
     1981 agcccaagct gcccgtcgag gtccctggga gcagtttgca cgagatcgaa gccgctttgc
     2041 tcgacgcatt gcccaggcag aggagaagct gggtccctac cttacccctg attccagggc
     2101 cagagcatgg gcacgcctta gaaacccatc tcttccacag tccgagcctc gctcttcctc
     2161 tgaggccact cccttgaccc aagatgtgac cacaccctct ccccttccca gtgaaacccc
     2221 ttcgcccagc ctgtacttgg gagggaggcg gggctaagcc tgagtagttt cctattattt
     2281 atttatttat ttatttgaat aagaaataaa gccttttaat ttgtagtgat aaaaaaaaaa
     2341 aaaaa
//
