LOCUS       NP_001355756             976 aa            linear   ROD 06-AUG-2024
DEFINITION  protein phosphatase 1 regulatory subunit 12B isoform 2 [Mus
            musculus].
ACCESSION   NP_001355756 XP_006529773
VERSION     NP_001355756.1
DBSOURCE    REFSEQ: accession NM_001368827.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 976)
  AUTHORS   Ye,Z., Okamoto,R., Ito,H., Ito,R., Moriwaki,K., Ichikawa,M.,
            Kimena,L., Ali,Y., Ito,M., Gomez-Sanchez,C.E. and Dohi,K.
  TITLE     Myosin Light Chain Phosphatase Plays an Important Role in Cardiac
            Fibrosis in a Model of Mineralocorticoid Receptor-Associated
            Hypertension
  JOURNAL   J Am Heart Assoc 13 (5), e032828 (2024)
   PUBMED   38420846
  REMARK    GeneRIF: Myosin Light Chain Phosphatase Plays an Important Role in
            Cardiac Fibrosis in a Model of Mineralocorticoid
            Receptor-Associated Hypertension.
REFERENCE   2  (residues 1 to 976)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 976)
  AUTHORS   Lee,E., May,H., Kazmierczak,K., Liang,J., Nguyen,N., Hill,J.A.,
            Gillette,T.G., Szczesna-Cordary,D. and Chang,A.N.
  TITLE     The MYPT2-regulated striated muscle-specific myosin light chain
            phosphatase limits cardiac myosin phosphorylation in vivo
  JOURNAL   J Biol Chem 300 (2), 105652 (2024)
   PUBMED   38224947
  REMARK    GeneRIF: The MYPT2-regulated striated muscle-specific myosin light
            chain phosphatase limits cardiac myosin phosphorylation in vivo.
REFERENCE   4  (residues 1 to 976)
  AUTHORS   Hu,T., Kalyanaraman,H., Pilz,R.B. and Casteel,D.E.
  TITLE     Phosphatase regulatory subunit MYPT2 knockout partially compensates
            for the cardiac dysfunction in mice caused by lack of myosin light
            chain kinase 3
  JOURNAL   J Biol Chem 299 (4), 104584 (2023)
   PUBMED   36889588
  REMARK    GeneRIF: Phosphatase regulatory subunit MYPT2 knockout partially
            compensates for the cardiac dysfunction in mice caused by lack of
            myosin light chain kinase 3.
REFERENCE   5  (residues 1 to 976)
  AUTHORS   Liu,K., Zhao,J., Chen,C., Xu,J., Bell,R.L., Hall,F.S., Koob,G.F.,
            Volkow,N.D., Qing,H. and Lin,Z.
  TITLE     Epistatic evidence for gender-dependant slow neurotransmission
            signalling in substance use disorders: PPP1R12B versus PPP1R1B
  JOURNAL   EBioMedicine 61, 103066 (2020)
   PUBMED   33096475
  REMARK    GeneRIF: Epistatic evidence for gender-dependant slow
            neurotransmission signalling in substance use disorders: PPP1R12B
            versus PPP1R1B.
REFERENCE   6  (residues 1 to 976)
  AUTHORS   Hughes,J.J., Alkhunaizi,E., Kruszka,P., Pyle,L.C., Grange,D.K.,
            Berger,S.I., Payne,K.K., Masser-Frye,D., Hu,T., Christie,M.R.,
            Clegg,N.J., Everson,J.L., Martinez,A.F., Walsh,L.E., Bedoukian,E.,
            Jones,M.C., Harris,C.J., Riedhammer,K.M., Choukair,D.,
            Fechner,P.Y., Rutter,M.M., Hufnagel,S.B., Roifman,M., Kletter,G.B.,
            Delot,E., Vilain,E., Lipinski,R.J., Vezina,C.M., Muenke,M. and
            Chitayat,D.
  TITLE     Loss-of-Function Variants in PPP1R12A: From Isolated Sex Reversal
            to Holoprosencephaly Spectrum and Urogenital Malformations
  JOURNAL   Am J Hum Genet 106 (1), 121-128 (2020)
   PUBMED   31883643
REFERENCE   7  (residues 1 to 976)
  AUTHORS   Mizutani,H., Okamoto,R., Moriki,N., Konishi,K., Taniguchi,M.,
            Fujita,S., Dohi,K., Onishi,K., Suzuki,N., Satoh,S., Makino,N.,
            Itoh,T., Hartshorne,D.J. and Ito,M.
  TITLE     Overexpression of myosin phosphatase reduces Ca(2+) sensitivity of
            contraction and impairs cardiac function
  JOURNAL   Circ J 74 (1), 120-128 (2010)
   PUBMED   19966500
  REMARK    GeneRIF: MYPT2 is truly the regulatory subunit of cardiac myosin
            phosphatase in-vivo and plays a significant role in modulating
            cardiac function.
REFERENCE   8  (residues 1 to 976)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   9  (residues 1 to 976)
  AUTHORS   Arimura,T., Suematsu,N., Zhou,Y.B., Nishimura,J., Satoh,S.,
            Takeshita,A., Kanaide,H. and Kimura,A.
  TITLE     Identification, characterization, and functional analysis of
            heart-specific myosin light chain phosphatase small subunit
  JOURNAL   J Biol Chem 276 (9), 6073-6082 (2001)
   PUBMED   11067852
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117827.6 and AC131591.13.
            
            On Mar 1, 2019 this sequence version replaced XP_006529773.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164139
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 58.24 cM"
     Protein         1..976
                     /product="protein phosphatase 1 regulatory subunit 12B
                     isoform 2"
                     /EC_number="3.1.3.53"
                     /note="myosin phosphatase target subunit 2; myosin
                     phosphatase-targeting subunit 2; protein phosphatase 1,
                     regulatory (inhibitor) subunit 12B"
                     /calculated_mol_wt=108919
     Region          1..52
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Site            29
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          57..86
                     /region_name="ANK 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          <62..290
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(67..69,71..72,76,79,88,90,92,96..97,100..102,
                     104..105,109,112,121,123,125,129..130,133..135,137..138,
                     142,145,154)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..119
                     /region_name="ANK 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          123..154
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..152
                     /region_name="ANK 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          156..247
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <169..>257
                     /region_name="PLN03192"
                     /note="Voltage-dependent potassium channel; Provisional"
                     /db_xref="CDD:215625"
     Region          216..245
                     /region_name="ANK 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          249..280
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          249..278
                     /region_name="ANK 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          342..489
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Site            444
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Site            502
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          503..873
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          608..663
                     /region_name="IPD_MYPT1"
                     /note="inhibitory phosphorylation domain of myosin
                     phosphatase targeting subunit 1(MYPT1); cd21944"
                     /db_xref="CDD:412019"
     Site            645
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:O60237; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Site            802
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Site            833
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     Region          875..976
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     Site            941
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BG95.2)"
     CDS             1..976
                     /gene="Ppp1r12b"
                     /gene_synonym="1810037O03Rik; 9530009M10Rik; Mypt2"
                     /coded_by="NM_001368827.1:159..3089"
                     /note="isoform 2 is encoded by transcript variant 3"
                     /db_xref="CCDS:CCDS87881.1"
                     /db_xref="GeneID:329251"
                     /db_xref="MGI:MGI:1916417"
ORIGIN      
        1 maelehlggk raesararra eqlrrwrgsl teqepaerqg agrqlqtrrg sprvrfedga
       61 vflaacssgd tdevkkllar gadintvnvd gltalhqaci denldmvkfl venranvnqq
      121 dnegwtplha aascgylnia eyfishgasv givnsegevp sdlaeepamk dllleqvkkq
      181 gvdleqsrke eeqqmlqdar qwlnsgried vrqarsgata lhvaaakgys evlrlliqag
      241 yelnvqdhdg wtplhaaahw gvkeacsila ealcdmdirn klgqtpfdva deglvehlem
      301 lqkkqdvlrs eketrnklie sdlnskfqsg lfknkekmly eeeipksqdt eeenkessss
      361 sseeeegede vsesetekea dkkpeatvnh snseiksrim eqipapaqnt fsassarrls
      421 slfnkaeepk despsswrlg lrktgshnml sevansreal rdrgssiyrs sssprisall
      481 ddkdkerenk syfsmlvprr lsstsdieek enresavnlv rsgshtrqlw rdeakgsetp
      541 qtiapstyts tylkrtpyks qadstaekta dsvssstplc vitnrpapst angvpaatvf
      601 ssagtdpsve arekrrsylt pvrdeeaesl rkarsrqarq trrstqgvtl tdlqeaektf
      661 srsraerqaq eqpgekledp gglegstkkq epsaaptkga gegrsleeep iyhrlryptq
      721 pdkpttpvsp sasrpslytg shllrtsras gpdsensets thataakemd tsekgeadld
      781 dqssnrlsvr errrakdrrr gtginfwtnd edetdvseev kealherlsr lesggtnpts
      841 sdsysdrasa rarrearear lasltsrvee dsnrdykkly esaltenqkl ktklqeaqle
      901 ladikaklek maqqkqekts drssvlevek rerralerkm semeeemkvl telksdnqrl
      961 kdengalirv isklsk
//
