LOCUS       NM_001368736            6084 bp    mRNA    linear   ROD 03-SEP-2024
DEFINITION  Mus musculus protein phosphatase 1, regulatory subunit 12A
            (Ppp1r12a), transcript variant 1, mRNA.
ACCESSION   NM_001368736 XM_006513326
VERSION     NM_001368736.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Wang,Y., Jiang,Z.H., Zhou,Y.W., Qiu,T.T., Wang,H., Zhu,M.S.,
            Chen,X. and Zhang,X.N.
  TITLE     Gallbladder dysfunction caused by MYPT1 ablation triggers
            cholestasis-induced hepatic fibrosis in mice
  JOURNAL   Hepatol Commun 8 (7), e0473 (2024)
   PUBMED   38934703
  REMARK    GeneRIF: Gallbladder dysfunction caused by MYPT1 ablation triggers
            cholestasis-induced hepatic fibrosis in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Chen,J., Li,C.G., Yang,L.X., Qian,Y., Zhu,L.W., Liu,P.Y., Cao,X.,
            Wang,Y., Zhu,M.S. and Xu,Y.
  TITLE     MYPT1SMKO Mice Function as a Novel Spontaneous Age- and
            Hypertension-Dependent Animal Model of CSVD
  JOURNAL   Transl Stroke Res 15 (3), 606-619 (2024)
   PUBMED   36843141
  REMARK    GeneRIF: MYPT1[SMKO] Mice Function as a Novel Spontaneous Age- and
            Hypertension-Dependent Animal Model of CSVD.
REFERENCE   3  (bases 1 to 6084)
  AUTHORS   Nagaoka,T., Katsuno,T., Fujimura,K., Tsuchida,K. and Kishi,M.
  TITLE     Functional interaction between Vangl2 and N-cadherin regulates
            planar cell polarization of the developing neural tube and cochlear
            sensory epithelium
  JOURNAL   Sci Rep 13 (1), 3905 (2023)
   PUBMED   36890135
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6084)
  AUTHORS   Takahashi,H., Yang,G., Yoneshiro,T., Abe,Y., Ito,R., Yang,C.,
            Nakazono,J., Okamoto-Katsuyama,M., Uchida,A., Arai,M., Jin,H.,
            Choi,H., Tumenjargal,M., Xie,S., Zhang,J., Sagae,H., Zhao,Y.,
            Yamaguchi,R., Nomura,Y., Shimizu,Y., Yamada,K., Yasuda,S.,
            Kimura,H., Tanaka,T., Wada,Y., Kodama,T., Aburatani,H., Zhu,M.S.,
            Inagaki,T., Osborne,T.F., Kawamura,T., Ishihama,Y., Matsumura,Y.
            and Sakai,J.
  TITLE     MYPT1-PP1beta phosphatase negatively regulates both chromatin
            landscape and co-activator recruitment for beige adipogenesis
  JOURNAL   Nat Commun 13 (1), 5715 (2022)
   PUBMED   36175407
  REMARK    GeneRIF: MYPT1-PP1beta phosphatase negatively regulates both
            chromatin landscape and co-activator recruitment for beige
            adipogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6084)
  AUTHORS   Zhao,W., Sun,J., Yao,L.Y., Hang,D., Li,Y.Q., Chen,C.P., Zhou,Y.W.,
            Chen,X., Tao,T., Wei,L.S., Zheng,Y.Y., Ge,X., Li,C.J., Xin,Z.C.,
            Pan,Y., Wang,X.Z., He,W.Q., Zhang,X.N., Yao,B. and Zhu,M.S.
  TITLE     MYPT1 reduction is a pathogenic factor of erectile dysfunction
  JOURNAL   Commun Biol 5 (1), 744 (2022)
   PUBMED   35879418
  REMARK    GeneRIF: MYPT1 reduction is a pathogenic factor of erectile
            dysfunction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6084)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 6084)
  AUTHORS   Arimura,T., Suematsu,N., Zhou,Y.B., Nishimura,J., Satoh,S.,
            Takeshita,A., Kanaide,H. and Kimura,A.
  TITLE     Identification, characterization, and functional analysis of
            heart-specific myosin light chain phosphatase small subunit
  JOURNAL   J Biol Chem 276 (9), 6073-6082 (2001)
   PUBMED   11067852
REFERENCE   8  (bases 1 to 6084)
  AUTHORS   Machida,H., Ito,M., Okamoto,R., Shiraki,K., Isaka,N.,
            Hartshorne,D.J. and Nakano,T.
  TITLE     Molecular cloning and analysis of the 5'-flanking region of the
            human MYPT1 gene
  JOURNAL   Biochim Biophys Acta 1517 (3), 424-429 (2001)
   PUBMED   11342221
REFERENCE   9  (bases 1 to 6084)
  AUTHORS   Tate,P., Lee,M., Tweedie,S., Skarnes,W.C. and Bickmore,W.A.
  TITLE     Capturing novel mouse genes encoding chromosomal and other nuclear
            proteins
  JOURNAL   J Cell Sci 111 (Pt 17), 2575-2585 (1998)
   PUBMED   9701556
REFERENCE   10 (bases 1 to 6084)
  AUTHORS   Kimura,K., Ito,M., Amano,M., Chihara,K., Fukata,Y., Nakafuku,M.,
            Yamamori,B., Feng,J., Nakano,T., Okawa,K., Iwamatsu,A. and
            Kaibuchi,K.
  TITLE     Regulation of myosin phosphatase by Rho and Rho-associated kinase
            (Rho-kinase)
  JOURNAL   Science 273 (5272), 245-248 (1996)
   PUBMED   8662509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139335.3.
            
            On Feb 22, 2019 this sequence version replaced XM_006513326.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-539               AC139335.3         15150-15688
            540-670             AC139335.3         51885-52015
            671-789             AC139335.3         67440-67558
            790-949             AC139335.3         83489-83648
            950-1094            AC139335.3         87051-87195
            1095-1169           AC139335.3         93157-93231
            1170-1258           AC139335.3         93831-93919
            1259-1416           AC139335.3         94173-94330
            1417-1541           AC139335.3         96058-96182
            1542-1757           AC139335.3         102487-102702
            1758-1852           AC139335.3         103966-104060
            1853-1957           AC139335.3         104892-104996
            1958-2125           AC139335.3         105809-105976
            2126-2296           AC139335.3         106377-106547
            2297-2387           AC139335.3         112808-112898
            2388-2588           AC139335.3         113888-114088
            2589-2760           AC139335.3         114297-114468
            2761-2882           AC139335.3         115362-115483
            2883-2948           AC139335.3         117755-117820
            2949-2965           AC139335.3         120456-120472
            2966-3101           AC139335.3         121624-121759
            3102-3206           AC139335.3         122306-122410
            3207-3254           AC139335.3         124774-124821
            3255-3305           AC139335.3         127190-127240
            3306-6084           AC139335.3         130259-133037
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 56.33 cM"
     gene            1..6084
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="protein phosphatase 1, regulatory subunit 12A"
                     /db_xref="GeneID:17931"
                     /db_xref="MGI:MGI:1309528"
     exon            1..539
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             303..3392
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myosin phosphatase target subunit 1; myosin
                     phosphatase-targeting subunit 1; protein phosphatase 1,
                     regulatory (inhibitor) subunit 12A"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 12A
                     isoform 1"
                     /protein_id="NP_001355665.1"
                     /db_xref="CCDS:CCDS88083.1"
                     /db_xref="GeneID:17931"
                     /db_xref="MGI:MGI:1309528"
                     /translation="MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVF
                     LAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQP
                     DNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVN
                     RQGVDIEAARKEEERVMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLL
                     IQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDI
                     LGYLEELQKKQTLLHSEKRDKKSPLIESTANMENNQPQKAFKNKETLIIEPEKNASRI
                     ESLEHEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVSNAHTSSTQAAP
                     AAVTAPTLSSNQGTPTSPVKKFPISTTKISPKEEERKDESPASWRLGLRKTGSYGALA
                     EISASKEAQKEKDTAGVMRSASSPRLSSSLDNKEKEKDNKGTRLAYVTPTIPRRLAST
                     SDIEEKENRESSSLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSCSFGRRQDDLISCS
                     VPSTTSTPTVTSAAGLQRSLPSSTSTAAKTPPGSSSAGTQSSTSNRLWAEDSTEKEKD
                     SAPTAVTIPVAPTVVNAAAPSTTTLTTTTAGTVSEVRERRRSYLTPVRDEESESQRKA
                     RSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEE
                     KKESEASREDEYKQKYSRTYDETYTRYRPVSTSSSSAPSSSSLSTLGSTLYASSQLNR
                     PNSLVGITSAYSRGLAKENEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSF
                     WTQDSDENEQERQSDTEDGSSKRETQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLE
                     DRKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQER
                     FADRSQLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK"
     exon            540..670
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            671..789
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..949
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1094
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1169
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1258
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1416
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1541
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1757
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1852
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1957
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2125
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2296
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2387
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2588
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..2760
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2882
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..2948
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..2965
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2966..3101
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3102..3206
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3254
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3305
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3306..6084
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5443..5448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="hexamer: AATAAA"
     polyA_site      5487
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="major polyA site"
     regulatory      5723..5728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="hexamer: CATAAA"
     polyA_site      5745
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="major polyA site"
     regulatory      5835..5840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="hexamer: CATAAA"
     polyA_site      5864
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="major polyA site"
     regulatory      6060..6065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
                     /note="hexamer: AGTAAA"
     polyA_site      6084
                     /gene="Ppp1r12a"
                     /gene_synonym="1200015F06Rik; 5730577I22Rik; D10Ertd625e;
                     Mypt1"
ORIGIN      
        1 agccgggccg cggctgacgg actgacgggc gggcggccgt gccgggggcg ggagggcgtc
       61 tgtggtagga agagaggccc gctcggccgg gctcgcccca gtgctctgtg ggatactgga
      121 agtctcgagc gtctcctccc cggctcgcgg ctccgctccg ctccggcccc tctctctctc
      181 tctctctctc tttctgtcgc cgacgcggtc gcatactccc cccggcggcg tcccctcgcc
      241 gccgccgccc ccttcccccc cgccccagct cgcgataaga ggagccggcg gctggagagg
      301 ggatgaagat ggcggacgcg aagcagaagc ggaacgagca gctgaagcgc tggatcggct
      361 ccgagacgga cctcgagccg cccgtggtga agcgccagaa gaccaaggtg aagttcgacg
      421 atggcgccgt cttcctcgcc gcctgctcca gcggcgacac ggacgaggtc ctcaagctgc
      481 tgcaccgcgg cgccgacatc aattacgcca atgtggacgg actcaccgcc ctgcaccagg
      541 cttgcattga tgacaatgtt gatatggtga agtttctggt agaaaatgga gcaaatatca
      601 atcaacctga caatgaaggc tggataccac tccatgcagc tgcttcctgt ggatatctcg
      661 atattgcaga gtttttgatt ggtcaaggag cacatgtagg agctgtcaac agtgaaggag
      721 acacaccttt agatattgca gaggaggagg cgatggaaga gctacttcaa aatgaggtta
      781 atcggcaagg tgttgatata gaagcggctc ggaaagagga ggagcgggta atgctaagag
      841 acgcgaggca gtggttaaac agcggccaca tcagtgacgt ccggcatgca aagtccgggg
      901 gcacagcact ccacgtggcg gccgccaaag gctatacaga agtgttaaaa cttttaatac
      961 aggcaggcta tgatgttaat attaaagatt atgatggctg gacacctctt catgctgcag
     1021 ctcattgggg taaagaagaa gcatgtcgga ttttagtgga caatctgtgt gatatggaga
     1081 cggtcaacaa agtgggccaa acagcctttg atgtagcaga tgaagacatt ttgggatatc
     1141 tagaagagtt gcaaaaaaaa caaactctgc tccatagtga gaagcgggat aagaaatctc
     1201 cactgatcga gtccacagca aatatggaaa acaatcaacc acagaaggct tttaaaaaca
     1261 aggaaacgtt gattattgag ccagagaaaa atgcatctcg catcgagtct ctggagcatg
     1321 agaaggctga tgaggaggaa gaaggcaaga aggacgagtc gagctgttcc agtgaggagg
     1381 acgaggagga cgattcagag tcagaggcag agacagataa gacaaagccc atggcttctg
     1441 taagcaatgc tcatacttcc agcacgcagg cagcccctgc agctgttaca gcacctactc
     1501 tgtcttccaa ccaggggacc cctacatcac ctgttaaaaa gtttccaata tcgactacaa
     1561 aaatttctcc caaagaagag gaaagaaaag atgagtctcc tgcatcctgg aggttagggc
     1621 ttcgaaagac tggcagttac ggtgccctgg ctgaaatcag tgcgtctaaa gaagcccaga
     1681 aggagaaaga cactgcaggg gtgatgcggt cggcttccag tccgagactc tcgtcctctt
     1741 tggataataa agaaaaggag aaagataaca aaggaacgag acttgcgtat gtcaccccta
     1801 caatcccgag acgactagcc agtacatctg acattgaaga gaaggagaac agagagtctt
     1861 caagtttgcg aacaagtagt tcttacacaa gaagaaaatg ggaagatgat cttaaaaaga
     1921 atagttcgat caatgaagga tccacttacc atagaagttg ctcctttggt agaagacaag
     1981 atgatttgat tagttgtagt gttccaagca ccacatcaac accaacagtt acctctgcag
     2041 cggggcttca gagaagcctc ccttccagca caagcactgc tgcaaagact cccccgggtt
     2101 cctcctccgc aggcacacag agcagtacct caaatcgttt gtgggctgag gatagtactg
     2161 agaaagaaaa ggacagtgct cctactgcag tgaccattcc tgtggctcca actgttgtaa
     2221 atgctgcagc tccttccacc accaccctga ctacaactac tgctggcact gtttccgagg
     2281 tcagggagag acgcaggtca tacctcactc ctgttaggga cgaagagtct gaatcccaaa
     2341 gaaaagcaag atctagacaa gcaagacagt ctagacggtc aacacagggg gtaacactga
     2401 ctgatcttca ggaagctgaa aaaacaatag gaagaagtcg ttctacgaga accagagaac
     2461 aagaaaatga agagaaagaa aaagaagaaa aggaaaagca ggataaagag aaacaagaag
     2521 aaaagaagga gtcagaagca tctagagaag atgaatataa acaaaagtat tcaagaacat
     2581 acgatgagac ttacacacgt tatagacctg tgtcaacttc aagttcaagc gctccgtcgt
     2641 cgtcctcact ctctactcta ggcagcacac tctatgcctc aagtcagctc aacaggccaa
     2701 acagtcttgt aggtataacc tctgcctact cccggggatt agccaaagaa aacgagagag
     2761 agggagagaa aaaagaagag gagaaagaag gggaagataa gtcacagcct aagtcaatca
     2821 gagaacgacg gcgacccaga gaaaagcgac ggtctactgg agtctccttc tggacacaag
     2881 atagtgatga aaatgagcaa gagcggcaat cggacactga ggacggctcc agcaagagag
     2941 agacgcagac ggattctgtt tccaggtatg acagcagttc cacatcatca agtgatcggt
     3001 atgattcctt gctgggtcgc tctgcttcat acagttactt agaagacaga aaaccttaca
     3061 gtagcaggct agaaaaggat gactcaactg acttcaaaaa gctttatgaa caaattttag
     3121 ctgaaaatga aaagctaaaa gcacagctac atgatacaaa tatggaacta acggatctaa
     3181 agttgcagtt ggaaaaggct acccagagac aagaacgatt tgctgacagg tcacaattgg
     3241 agatggaaaa aagggaacga agagctctag aaagaagaat atctgaaatg gaagaagagc
     3301 tcaaaatgtt accagactta aaggcagaca accagaggct aaaggatgaa aatggagcct
     3361 tgatcagagt tataagcaaa ctttccaagt aggacaggag gcagagaagc aaagcagcag
     3421 gacttgcaca ctccccagtg gaccacagtg gcagtcactg gacgccggag gaaagaactc
     3481 acgggagact gtcattttcc gatatcctgc caaacgccct cttatctagg agttttgttt
     3541 cgtttaattt tctgcccacc ccgttggtta tcaagaccat tgtttcatgt taaagcagct
     3601 gctgagaaga ttttttttca atgactgaga aaacttgttt acagctccag caaataaaga
     3661 aagtgttcaa ggccagatat gcctcagata tttaacccgt aagccttagt tgtatataaa
     3721 tacttttgtg tcaacaaaaa cttccagctc tcacagaaga cagttagtca acaggttttt
     3781 ttgttgttgg gttttttttt tctttttctt tttctttttt ctttttgatg tgccacagtt
     3841 tctagtttct caatatttaa attgtttgct ttccatctga gtttgggttt gtattttttt
     3901 tttccttcta actcttcatg tgtcagagtc tctctatgtt cacagccttc tcatttacag
     3961 aaaacttggc tcttctgagc gtcttgtcac gtgttaggct gacgtgttct ctcccacctg
     4021 gaactgaatt gtggtgggac acttgctgtc actgtttgtg caatacgcat ttatttctta
     4081 catgagtgct ttaacgtcca ttgaaatttg atctttccag tcctatcttc tgccctcatc
     4141 tgtcactttt tgttttttat tgtagtataa gatgctagat gctgtacttt tcacattcat
     4201 tttagcaggt actgagtgac gctgtatata taaataagtg tattgttttg atttttagac
     4261 caccacatgg catgcttgac tattttctta attcagatgt ctgcagtgca cagtgggcta
     4321 ctccataaag gtctttagag cagagctcgc tcacagtggg caggaggacc tgaaagattc
     4381 tgcggtttat gggacctccc cttccaagct tttctactgt aaactgcttt gctttttttt
     4441 tcattcggta gtagtcagac ctgattacaa agtagagaat cgcttcacat ttcacacgcc
     4501 tatatttatc tgagtgctgt ctgaaactgt tgtgctagcc acagtagtgc taggagatcc
     4561 tttgcagact taaatctgtg agtggtgtta agtgcactgt tattgccatg tgagggggca
     4621 tcaactctga tcccaccacc atgttcagac cgtttttata catttcagtg gccttttttt
     4681 aaggaaaaaa ctaagcaaaa tgaagtccat aatgaaagtg gcttttattt ggacaaataa
     4741 cttttatatt ttcatcaaac catgcaaaaa atatatatat attacatctt cctggcacat
     4801 aactgtctcc ttaaccattg aacagttcag ccattcgaat aaattgtaca tttaaagcgt
     4861 atagtagctg actgtattat tgagtgtatt gtatactata ttaaatgtga atttgtaccc
     4921 ttccatttta aaaggtcatt gtgttctact gcctatttta gattacttcg gggatgggaa
     4981 agggtgtttt ggtaagtaga atttaagtcc tctctcttga ttggttttat gaggatataa
     5041 ggttatactc ttactaccaa gctcaggatt cttttaaagg tacaaggata ggtgtgagga
     5101 tttatttttg gttatatttg aactgtatga atggtgggtc tgaaaacaga gtatttcacg
     5161 gtctagtgag gaggtaaact atgcggctag ttgatcagtg tgagtgactg cagcgtgttc
     5221 tgggtgcaca acagttaggc atgctgtggt ttgtatctgt gaagtctgga tgcctgagaa
     5281 gaatgagtaa atgcgtgttt ctaatatttt agtgttttgt atttaggtta tttattcttt
     5341 gtatctaaag ttgaacagct actgtgctat attgacttta ttggtaggac tgagcagacc
     5401 ttatttctgc atgaaactag ttgcaaaacc cactattttg gaaataaaaa tgtattttga
     5461 catatacaaa taaaatgaat aaaactattt taaatgtgtg aacttttaat gaaaacagtt
     5521 taaatcttgt tctgagatat ttaaattctg catgggtatt ttttcactaa atgctttact
     5581 tggataacag tttgaataat ttttgaaatt gtaatattgt gataatttgc acagcaatgt
     5641 acatgctcat ctgtaaatgc gaacttaact gccatattta tgcaatctgt gtaccaattt
     5701 ggacaaatgt ttatgtattt tacataaagc tgtctgtatg cagaatctga gagctagttt
     5761 ttttatgcca agtggacaaa aataatggta attaagattc aagatagttg ctttgtagca
     5821 tgagattcca tttccataaa catttgcagt gtttaaaaac taaacttctg gttttttcct
     5881 tagctctaga ctcaggcata agaacataca caaagatact attccactga aattttaaat
     5941 cctttccact accattattt tccatcttgg gtttctcgag ggttcagtcc acccgcacag
     6001 ctcgaggtgg ctgagttgcg cttttaactt ctcatgtggg cctttgggca ggaacctgaa
     6061 gtaaaccatg ttcttttctc tgga
//
