LOCUS       NM_175523               5621 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus protein phosphatase 1K (PP2C domain containing)
            (Ppm1k), transcript variant 1, mRNA.
ACCESSION   NM_175523
VERSION     NM_175523.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Lu,T., Zheng,Y., Chen,X., Lin,Z., Liu,C. and Yuan,C.
  TITLE     miR-743b-3p promotes hepatic lipogenesis via branched-chain amino
            acids (BCAA) metabolism by targeting PPM1K in aged mice
  JOURNAL   Arch Gerontol Geriatr 123, 105424 (2024)
   PUBMED   38565071
  REMARK    GeneRIF: miR-743b-3p promotes hepatic lipogenesis via
            branched-chain amino acids (BCAA) metabolism by targeting PPM1K in
            aged mice.
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Li,T., Zhao,L., Li,Y., Dang,M., Lu,J., Lu,Z., Huang,Q., Yang,Y.,
            Feng,Y., Wang,X., Jian,Y., Wang,H., Guo,Y., Zhang,L., Jiang,Y.,
            Fan,S., Wu,S., Fan,H., Kuang,F. and Zhang,G.
  TITLE     PPM1K mediates metabolic disorder of branched-chain amino acid and
            regulates cerebral ischemia-reperfusion injury by activating
            ferroptosis in neurons
  JOURNAL   Cell Death Dis 14 (9), 634 (2023)
   PUBMED   37752100
  REMARK    GeneRIF: PPM1K mediates metabolic disorder of branched-chain amino
            acid and regulates cerebral ischemia-reperfusion injury by
            activating ferroptosis in neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5621)
  AUTHORS   Yao,C.C., Sun,R.M., Yang,Y., Zhou,H.Y., Meng,Z.W., Chi,R.,
            Xia,L.L., Ji,P., Chen,Y.Y., Zhang,G.Q., Sun,H.P., Lu,S., Yang,C.
            and Wang,Y.
  TITLE     Accumulation of branched-chain amino acids reprograms glucose
            metabolism in CD8+ T cells with enhanced effector function and
            anti-tumor response
  JOURNAL   Cell Rep 42 (3), 112186 (2023)
   PUBMED   36870057
REFERENCE   4  (bases 1 to 5621)
  AUTHORS   Mu,L., Ye,Z., Hu,J., Zhang,Y., Chen,K., Sun,H., Li,R., Mao,W.,
            Long,X., Zhang,C., Lai,Y., Liu,J., Zhao,Y. and Qiao,J.
  TITLE     PPM1K-regulated impaired catabolism of branched-chain amino acids
            orchestrates polycystic ovary syndrome
  JOURNAL   EBioMedicine 89, 104492 (2023)
   PUBMED   36863088
  REMARK    GeneRIF: PPM1K-regulated impaired catabolism of branched-chain
            amino acids orchestrates polycystic ovary syndrome.
REFERENCE   5  (bases 1 to 5621)
  AUTHORS   Bozadjieva Kramer,N., Evers,S.S., Shin,J.H., Silverwood,S.,
            Wang,Y., Burant,C.F., Sandoval,D.A. and Seeley,R.J.
  TITLE     The Role of Elevated Branched-Chain Amino Acids in the Effects of
            Vertical Sleeve Gastrectomy to Reduce Weight and Improve Glucose
            Regulation
  JOURNAL   Cell Rep 33 (2), 108239 (2020)
   PUBMED   33053352
REFERENCE   6  (bases 1 to 5621)
  AUTHORS   Lu,G., Ota,A., Ren,S., Franklin,S., Rau,C.D., Ping,P., Lane,T.F.,
            Zhou,Z.H., Reue,K., Lusis,A.J., Vondriska,T. and Wang,Y.
  TITLE     PPM1l encodes an inositol requiring-protein 1 (IRE1) specific
            phosphatase that regulates the functional outcome of the ER stress
            response
  JOURNAL   Mol Metab 2 (4), 405-416 (2013)
   PUBMED   24327956
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5621)
  AUTHORS   Zimmerman,H.A., Olson,K.C., Chen,G. and Lynch,C.J.
  TITLE     Adipose transplant for inborn errors of branched chain amino acid
            metabolism in mice
  JOURNAL   Mol Genet Metab 109 (4), 345-353 (2013)
   PUBMED   23800641
REFERENCE   8  (bases 1 to 5621)
  AUTHORS   Zhou,M., Lu,G., Gao,C., Wang,Y. and Sun,H.
  TITLE     Tissue-specific and nutrient regulation of the branched-chain
            alpha-keto acid dehydrogenase phosphatase, protein phosphatase 2Cm
            (PP2Cm)
  JOURNAL   J Biol Chem 287 (28), 23397-23406 (2012)
   PUBMED   22589535
  REMARK    GeneRIF: Data show that show that PP2Cm expression is highly
            enriched in brain, liver, kidney, but low in skeletal muscle.
REFERENCE   9  (bases 1 to 5621)
  AUTHORS   Lu,G., Sun,H., She,P., Youn,J.Y., Warburton,S., Ping,P.,
            Vondriska,T.M., Cai,H., Lynch,C.J. and Wang,Y.
  TITLE     Protein phosphatase 2Cm is a critical regulator of branched-chain
            amino acid catabolism in mice and cultured cells
  JOURNAL   J Clin Invest 119 (6), 1678-1687 (2009)
   PUBMED   19411760
REFERENCE   10 (bases 1 to 5621)
  AUTHORS   Lu,G., Ren,S., Korge,P., Choi,J., Dong,Y., Weiss,J., Koehler,C.,
            Chen,J.N. and Wang,Y.
  TITLE     A novel mitochondrial matrix serine/threonine protein phosphatase
            regulates the mitochondria permeability transition pore and is
            essential for cellular survival and development
  JOURNAL   Genes Dev 21 (7), 784-796 (2007)
   PUBMED   17374715
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125121.4.
            
            On Jun 3, 2018 this sequence version replaced NM_175523.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092238.1, SRR9219381.4063.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-208               AC125121.4         11828-12035
            209-726             AC125121.4         22061-22578
            727-827             AC125121.4         24466-24566
            828-993             AC125121.4         31557-31722
            994-1138            AC125121.4         32197-32341
            1139-1273           AC125121.4         33239-33373
            1274-5621           AC125121.4         36466-40813
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 27.82 cM"
     gene            1..5621
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /note="protein phosphatase 1K (PP2C domain containing)"
                     /db_xref="GeneID:243382"
                     /db_xref="MGI:MGI:2442111"
     exon            1..208
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     exon            209..726
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     CDS             287..1405
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.52"
                     /note="protein phosphatase 1K, mitochondrial; protein
                     phosphatase 2C family member; branched-chain
                     alpha-ketoacid dehydrogenase phosphatase;
                     [3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring)]-phosphatase; protein
                     phosphatase Mn(2+)-dependent 1K"
                     /codon_start=1
                     /product="protein phosphatase Mn(2+)-dependent 1K
                     precursor"
                     /protein_id="NP_780732.1"
                     /db_xref="CCDS:CCDS20183.1"
                     /db_xref="GeneID:243382"
                     /db_xref="MGI:MGI:2442111"
                     /translation="MLSAAFITLLRSGGNQVKKRVLLSSILLQDHRQATPACYFSTSE
                     ARCSRFDPDGSGQPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLENVGCASLIG
                     KRKENEDRFGFAQLTEEVLYFAVYDGHGGPAAADFCHTHMEKCVMDLLPREKDLETVL
                     TLAFLEIDKAFASYAHLSADASLLTSGTTATVALLRDGVELVVASVGDSRALLCRKGK
                     PMKLTTDHTPERKDEKERIKKFGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKASGVIA
                     EPETTRIKLYHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPKEAAHSVTEQAIQYG
                     TEDNSTAVVVPFGAWGKYKNSEITFSFSRSFASSGRWA"
     exon            727..827
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     exon            828..993
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1138
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1273
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..5621
                     /gene="Ppm1k"
                     /gene_synonym="2900063A19Rik; A930026L03Rik; BCKDH; BDP;
                     PP2C-kappa; PP2Cm"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actcctccgg gtctccattt ggccagctgc ggttgatttt tttttatttt ccgagaattt
       61 ccaggagtgg aagagactgt ggcttcccta attgcttgtt ctggagatca taatcaacag
      121 agaagtcagt ctagtttatc atccaagaaa tccttccctt ccttccatta gccaattaag
      181 aaatttacag acacctaccc atcacaaggt acatcatttt tctgcacgga atactattat
      241 tgctctattt gtcttttcct gtgactgggg cctttggcat tgcattatgt tatcagcggc
      301 cttcattact ttgttgagaa gtggtgggaa tcaggtgaag aagagagtgt tgttaagctc
      361 catcctcctg caggaccaca ggcaggcgac tcctgcctgc tacttctcca cttcagaggc
      421 caggtgttct cggtttgatc cagatggaag tgggcagcca gccacttggg acaattttgg
      481 gatctgggat aaccgcattg atgagccaat tctgctgcca cctagcatca agtacggcaa
      541 accaattccc aaaattagcc tggagaacgt gggctgtgcc tctctcattg gcaaacggaa
      601 agaaaacgaa gatcgatttg ggtttgcaca gctgacagaa gaggtgctat acttcgcagt
      661 ctatgatgga catgggggcc ctgcagccgc tgacttctgt cacacacaca tggagaaatg
      721 tgttatggat ttgcttcctc gggagaaaga cttggaaact gtcctgacct tggcctttct
      781 agaaatagat aaagccttcg cgagttatgc ccacctgtct gcagatgcaa gcctcctgac
      841 ctctgggact actgcaacag tagccctgtt gagagatggt gttgaactgg tggtagccag
      901 tgttggagac agccgggctc ttttgtgtag aaaaggaaaa cccatgaagc tgaccactga
      961 ccatacccca gaaagaaaag atgagaaaga aaggatcaag aaattcggtg ggtttgtagc
     1021 ttggaatagt ttgggacagc cccatgtaaa tggcagactt gcaatgacaa ggagtatcgg
     1081 agatttggat cttaaagcca gtggggtaat tgcagaacct gagacaacaa ggattaagct
     1141 ctaccatgct gacgacagtt tcctggtcct taccacagat gggattaact tcatggtgaa
     1201 tagtcaagag atctgcgact ttgtcaacca gtgccacgat cctaaagaag cagctcattc
     1261 ggtgactgag caggcaatac agtacggtac cgaagacaac agcactgcag tagtggtgcc
     1321 ctttggtgct tggggaaaat acaagaactc tgaaattacc ttctcattca gcagaagctt
     1381 tgcctccagt gggagatggg cctgatcgac agctcagact cggagttcct atgcagcagt
     1441 gtcagtgggc ttatcaagaa actggtaaca cacagaaggc cacctgtacc cgtgtgtcat
     1501 tgtgactgat cgatcctaac agtataagct taccagccac atacccagta gtattttcat
     1561 aaatcctcat catcattatg gtcaactgca catgctcagt gtaagcatca taacacagct
     1621 gtgaagccgc tgtgcatctc tgagctgaat gggagaagaa gcggttttgg tccatttgat
     1681 gagtgatgaa tctgtgtttc ctttgtaagg ctagagattg aatctggggc tttctaagtt
     1741 tttaagacta agaggcagat ttagtttctt tttagtaagt ttgttcttta ttcagttgga
     1801 caggtatttt tttttttgaa ttttgatagc tggtcaatgt gttatttgca agtgttcctt
     1861 tttcctttct ctgtagaaag caggttttca ctggctgacc ttgaactcct gggttcaaat
     1921 ggtcttccta acgtcagctt cctgagtacc ttttaatttt ttttttttta aagcagaaca
     1981 agcatatttt ttcccaagaa gagtgagaac atatacctag gagatggggg cattccaagg
     2041 gagggataca agggatataa gagataaagg gatacaaggt acattttaat aaatgtacct
     2101 aggagttata ataaagccta ggttctctat gactgaatag tgtactctgg atcacttatt
     2161 agtaaattta accctgcaaa atacatggct aaagtgtata catatttata aatagctctc
     2221 ttgtgtattt taaatatttt tagcacttcc ccatccattt ctatttttgt ggagttaaat
     2281 atgctatata ttggtaactg ttcggtaagc tccagagaag aatattctga ggctttggtc
     2341 aaaatctgta aagtgaaaca ctgacagtgc caatcttgtg taagtttgtt gctgtgtggt
     2401 ttgtatgtag cgtttttata ccactttctg tgcacacagt aagcgagttg gggcctttgt
     2461 tagtaaagat aatattacaa tgacttatac tgctatagac atagcttggg gtttatgtct
     2521 ccaatttcct catttctaag attcatgcgg aaaaaaaaat cttttacaaa ggatattcaa
     2581 acatgtcagc ttgctatgta aattaggggt gtaaaaccac actccttttt acctgcatat
     2641 aaaattactg tcaaactgtg aatccctgaa tgtgtgcttg tagttaatct atattttcac
     2701 agggtaattt aaatttagta tatgtgcagt gagtgtatat atgtatattt tgaagtaata
     2761 taaaattaaa gggaacaatc ttgtggcgca tttatcagct agctgtaaat tattgatata
     2821 tttatctcta aggacactca ataacattga gtgtcaaatt ctctgtaata aagattcagt
     2881 tgttactggt agataaccac tatggattac aaataaatat gggggccagt attggtggcc
     2941 tctacctata atctcaggac ttaaggagga gacaagagaa tcgggatggt aactttgaaa
     3001 ttagcctgag ctacagagca agttccacgg tagcctgagc cacagcttgt ctccccgaac
     3061 aaagtatcag caacaaatgg ctgtaagaga cattttatta ggtgtcacgt atgtggataa
     3121 ccaaagaaca gaggcagaca tatcgttgtg tggtaattcg aatataaagt tatgggatat
     3181 gagaagaaca ggaaatgtca aaagtgactt ggaattttat ggcagaaaac aaaggaaaaa
     3241 taatgaaata tatatacctt caagtctgac atgttttctg cctttaactt ttgtttaaaa
     3301 tatttagtag aaataaagaa taagttctca ataaagaatt taagggggct ggtgagatgg
     3361 ctcagtggtt aggagcaccc gactgctctt ccgaaggtcc agagttcaaa tcccagcaac
     3421 cacatggtgg ctcacaacca tccgtaacaa gatctgactc cctcttctgg tgtgtctgaa
     3481 gacagctaca gtgtacttac atataataat aaataaatct ttaaaaacta aaattaaatt
     3541 aaattaaaaa aaaaagaatt taaggtcttt tcatggatgt cagttgaaag aaacaatgct
     3601 tttccagaca tgttagctta catgtgcagt tccaacatct ggggaggctg gggcacagtc
     3661 atcctgaata gcaagagccc aggtcaaggg acaaaataca cggaagagag tagaggacgt
     3721 gctcactgtc cgaggaagaa tggggagact agcagagctt aaaggccttc ttgttgagtc
     3781 tggtgttgaa aagttatgaa aacaacgtgt ttgtttgttt gtttgtttgt ttttttgttt
     3841 gttttttggt tttggtgttt tttttttttg gtgttttttt tttttttttt tttggttttt
     3901 caagacaggg tttctctgtg tagccctggc tgtcctggaa ctcactttgt agaccaggct
     3961 ggccttgaac tcagaaatct gcctgcctct gcctcccaag tactggaatt aaaggcatgt
     4021 gccaccacat cctggcttga aaacaacttt taagagtatg gagaacttgt aaactaatat
     4081 gcacaacaaa attctcaaac attaaaaatg acaacgtggt gtgtcttatt tgtttctgtt
     4141 ttgtgttctt cacattgctg gtctccctcc cccctttact ttgaaactag gattgagaat
     4201 gaccttgtga tgttcagagg tttgagggtc attcatagat gaaggcgagt tccagacctg
     4261 acaaggagga atctgtagat gatggccttg ggcgcatttg agcctgtttc ttctggaaaa
     4321 agcttttgat ggtggcggtg aggttaggag tgtgtaggtg tgtgtttaga cagacttgtt
     4381 ttaacctcct gagtgctgag attataggca tctgctttca tgcctgattc tacctgaaag
     4441 aaatttaaag cccaaattca gtcacccttg attaagcata aaaagtgctg ctctccatag
     4501 ggactgggct gcatcctttc ctgtagactt tcttaacaca catttgttaa gatcaaacct
     4561 gataaaacta catgttaata tgaaaaatac atgtgtcttt attttctaag tttttgtatt
     4621 aaaatgtaat ttaaagagta tattataatc ctttgtgata gtttataatc ctaaattctt
     4681 aagtcttttg tcaattcaga agtaaatgtg tcttgcttcc tgtcagctgt tcatgtgtta
     4741 ggattctttg tgtctcttag ctgcttcaaa tctaggctct ggtcctcatc tggggtcaaa
     4801 ggatccctta ggaggggtca gttttgcttc tgattgttat atgacatgaa ctttcccata
     4861 ttatatcatt taataatttt tattttgaag aggtaatagc atataatcta aaatacagaa
     4921 attatttaaa atttgactat tagttaattt tctaagactg gatatggaat taatctcatg
     4981 gtaacaacag gttttcatgt tatgagaatg tttcagggtg ggacctgggg agttcaagtt
     5041 tggagttaaa taaactctag ccatgtgttt gtgttttcca ttactgtgag ggttccccat
     5101 attcctgtgc atgtaaaaca cagggccata agatttgggc tgttccctta ctttcaattt
     5161 cttagttttc attatgagaa tggcttaagc aggaatagca agttgtgttt aacaagatga
     5221 agaaaccaaa tgttgacttg cctgctgctt ttggcagctg gaataagacc agtagtttct
     5281 gtggcagctc acactgattg tctcatggtt ggaatggccc atggtgtcac agaattctgg
     5341 actgtgggaa ctcagatgtg tcccaccact gagagcgagt gacctgtggt tgggttccag
     5401 tgcaaaagtc accactgtgg tataacctga attaactgaa tttctgctgc atttctcttg
     5461 tgactatttc tttcataatt tgccaaagta ctacttggta tagtatgttt gtagtgagtt
     5521 tgactattgt tttaaatgaa tgtgctagaa taactgtgaa caaactaaaa ttacctgttt
     5581 caatgttgaa aacatcttaa taaagtgcac attataaact c
//
