LOCUS       NM_001411569            2880 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus protein phosphatase 1B, magnesium dependent, beta
            isoform (Ppm1b), transcript variant 5, mRNA.
ACCESSION   NM_001411569 XM_011246321
VERSION     NM_001411569.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ishii,N., Homma,T., Watanabe,R., Kimura,N., Ohnishi,M.,
            Kobayashi,T. and Fujii,J.
  TITLE     A heterozygous deficiency in protein phosphatase Ppm1b results in
            an altered ovulation number in mice
  JOURNAL   Mol Med Rep 19 (6), 5353-5360 (2019)
   PUBMED   31059097
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Chen,W., Wu,J., Li,L., Zhang,Z., Ren,J., Liang,Y., Chen,F.,
            Yang,C., Zhou,Z., Su,S.S., Zheng,X., Zhang,Z., Zhong,C.Q., Wan,H.,
            Xiao,M., Lin,X., Feng,X.H. and Han,J.
  TITLE     Ppm1b negatively regulates necroptosis through dephosphorylating
            Rip3
  JOURNAL   Nat Cell Biol 17 (4), 434-444 (2015)
   PUBMED   25751141
  REMARK    GeneRIF: Ppm1b negatively regulates necroptosis through
            dephosphorylating Rip3 in vitro and in vivo.
REFERENCE   3  (bases 1 to 2880)
  AUTHORS   Tasdelen,I., van Beekum,O., Gorbenko,O., Fleskens,V., van den
            Broek,N.J., Koppen,A., Hamers,N., Berger,R., Coffer,P.J.,
            Brenkman,A.B. and Kalkhoven,E.
  TITLE     The serine/threonine phosphatase PPM1B (PP2Cbeta) selectively
            modulates PPARgamma activity
  JOURNAL   Biochem J 451 (1), 45-53 (2013)
   PUBMED   23320500
  REMARK    GeneRIF: the phosphatase PPM1B as a novel selective modulator of
            PPARgamma activity.
REFERENCE   4  (bases 1 to 2880)
  AUTHORS   Chida,T., Ando,M., Matsuki,T., Masu,Y., Nagaura,Y.,
            Takano-Yamamoto,T., Tamura,S. and Kobayashi,T.
  TITLE     N-Myristoylation is essential for protein phosphatases PPM1A and
            PPM1B to dephosphorylate their physiological substrates in cells
  JOURNAL   Biochem J 449 (3), 741-749 (2013)
   PUBMED   23088624
  REMARK    GeneRIF: Although a non-myristoylated mutation (G2A) of PPM1A and
            PPM1B prevented membrane association, this relocalization did not
            likely cause the decreased activity towards AMPKalpha.
REFERENCE   5  (bases 1 to 2880)
  AUTHORS   Sasaki,M., Ohnishi,M., Tashiro,F., Niwa,H., Suzuki,A., Miyazaki,J.,
            Kobayashi,T. and Tamura,S.
  TITLE     Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene
            leads to early pre-implantation lethality
  JOURNAL   Mech Dev 124 (6), 489-499 (2007)
   PUBMED   17499977
  REMARK    GeneRIF: These observations suggest that relatively high PP2Cbeta
            expression is specifically required during the early stages of
            pre-implantation development.
REFERENCE   6  (bases 1 to 2880)
  AUTHORS   Watanabe,T., Ohnishi,M., Kobayashi,T., Oishi,M. and Tamura,S.
  TITLE     The mouse protein phosphatase 2Cbeta (Ppm1b) gene maps to the
            distal part of mouse chromosome 17
  JOURNAL   Genomics 33 (1), 147-149 (1996)
   PUBMED   8617503
REFERENCE   7  (bases 1 to 2880)
  AUTHORS   Ohnishi,M., Nakagawara,K., Mori,M., Kobayashi,T., Kato,S.,
            Sasahara,Y., Kusuda,K., Chida,N., Kobayashi,T., Yanagawa,Y.,
            Hiraga,A., Takeuchi,T. and Tamura,S.
  TITLE     Localization of the mouse protein serine/threonine phosphatase 2C
            beta gene to chromosome 17E 4-5
  JOURNAL   Genomics 32 (1), 134-136 (1996)
   PUBMED   8786102
REFERENCE   8  (bases 1 to 2880)
  AUTHORS   Kato,S., Terasawa,T., Kobayashi,T., Ohnishi,M., Sasahara,Y.,
            Kusuda,K., Yanagawa,Y., Hiraga,A., Matsui,Y. and Tamura,S.
  TITLE     Molecular cloning and expression of mouse mg(2+)-dependent protein
            phosphatase beta-4 (type 2C beta-4)
  JOURNAL   Arch Biochem Biophys 318 (2), 387-393 (1995)
   PUBMED   7733667
REFERENCE   9  (bases 1 to 2880)
  AUTHORS   Hou,E.W., Kawai,Y., Miyasaka,H. and Li,S.S.
  TITLE     Molecular cloning and expression of cDNAs encoding two isoforms of
            protein phosphatase 2C beta from mouse testis
  JOURNAL   Biochem Mol Biol Int 32 (4), 773-780 (1994)
   PUBMED   8038726
REFERENCE   10 (bases 1 to 2880)
  AUTHORS   Terasawa,T., Kobayashi,T., Murakami,T., Ohnishi,M., Kato,S.,
            Tanaka,O., Kondo,H., Yamamoto,H., Takeuchi,T. and Tamura,S.
  TITLE     Molecular cloning of a novel isotype of Mg(2+)-dependent protein
            phosphatase beta (type 2C beta) enriched in brain and heart
  JOURNAL   Arch Biochem Biophys 307 (2), 342-349 (1993)
   PUBMED   8274020
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142103.3.
            
            On Aug 26, 2022 this sequence version replaced XM_011246321.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784643.416287.1,
                                           SRR17784650.944736.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-516               AC142103.3         70016-70531         c
            517-1376            AC142103.3         33124-33983         c
            1377-1494           AC142103.3         24280-24397         c
            1495-1606           AC142103.3         22640-22751         c
            1607-1664           AC142103.3         22097-22154         c
            1665-2880           AC142103.3         12882-14097         c
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 55.13 cM"
     gene            1..2880
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /note="protein phosphatase 1B, magnesium dependent, beta
                     isoform"
                     /db_xref="GeneID:19043"
                     /db_xref="MGI:MGI:101841"
     exon            1..516
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     exon            517..1376
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     CDS             531..1964
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /EC_number="3.1.3.16"
                     /note="isoform 1 is encoded by transcript variant 5;
                     PP2C-beta"
                     /codon_start=1
                     /product="protein phosphatase 1B isoform 1"
                     /protein_id="NP_001398498.1"
                     /db_xref="GeneID:19043"
                     /db_xref="MGI:MGI:101841"
                     /translation="MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVV
                     GIPHGLDNWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGSALEPSVESV
                     KTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMVSPTHMYFINCGDSRAVLCRNG
                     QVCFSTQDHKPCNPVEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQ
                     LVSPEPEVYEIVRAEEDEFVVLACDGIWDVMSNEELCEFVKSRLEVSDDLENVCNWVV
                     DTCLHKGSRDNMSVVLVCFSNAPKVSEEAVKRDSELDKHLESRVEEIMQKSGEEGMPD
                     LAHVMRILSAENIPNLPPGGGLAGKRHVIEAVYSRLNPHKDNDGASDEAEEGGSQGKL
                     VEALRQMRVNHRGNYRQLLEEMLTSYRLAKVEGEESPADPAAAAASSNSDGGNPVAMQ
                     ERDTEGGPAGLDSRNEDAGTKRSAENI"
     exon            1377..1494
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1606
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1664
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..2880
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2674..2679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /note="hexamer: AATAAA"
     polyA_site      2705
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /note="major polyA site"
     regulatory      2764..2769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /note="hexamer: AATAAA"
     polyA_site      2790
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
     regulatory      2857..2862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
                     /note="hexamer: ATTAAA"
     polyA_site      2880
                     /gene="Ppm1b"
                     /gene_synonym="PP2CB"
ORIGIN      
        1 gcactctcgc gtgcgcccca cccgtagttg aagcaaaaca acgcctctct ccaggccctt
       61 tctcgaacct ttctgagctc ttccttcaca gctctatgcc aaagagaggc ggccacttct
      121 tccaatgagg gtctaggggc acttctctgg ccaatcagaa gccggctctc tccactccgg
      181 gtgggaccaa tttccaccta taactggact gcccgttggt ttctttatgg tgttcgttct
      241 ccgcgaagtg tagaggtcag aactgcaggg tccgcagact cggggcttgg ccatctacga
      301 atactagcca atccctttta ggatactttt gcgcgttcat ttaaaaaaag ccgaagtgac
      361 ggacttggaa attggtttct tgacggacaa gtagaggaag ccaatagtcg gaggatcagg
      421 ccgccatttg aaccaatcag aaaaggatat cggtcttcga gttggggcgt aatttgactg
      481 acggaggact ctacctatgg ctactagggt gggcagattc attactaaac atgggtgcat
      541 ttttggataa acccaaaact gaaaagcaca atgctcacgg tgctgggaat ggtctgcgtt
      601 atggcctgag cagtatgcaa ggatggagag tagaaatgga agatgcacac acagctgttg
      661 tgggtattcc tcacggcttg gacaactggt cgttttttgc agtttatgac ggtcatgctg
      721 gatcccgagt ggcaaattac tgttcaacac atctattaga acacatcacc accaatgaag
      781 acttcagggc agctgacaaa tcaggctctg ctctcgagcc ttcagtagaa agtgtgaaga
      841 ctggtatcag gactggcttt ttgaaaattg atgaatatat gcgtaacttc tcagacctga
      901 ggaacgggat ggacaggagc ggctcgactg cagtgggcgt gatggtctcc cctacacaca
      961 tgtacttcat caactgtggt gactctcgag ctgttctgtg taggaacgga caggtctgct
     1021 tttctaccca ggatcacaaa ccttgtaatc cagtggagaa ggagcgcatc caaaacgcag
     1081 gaggcagtgt gatgatccag cgtgtgaacg gctcactagc agtgtctcgg gctctggggg
     1141 actatgacta caagtgtgtg gatggcaagg gccctacaga gcagcttgtt tctccagagc
     1201 ctgaggttta tgagattgtg agagcagaag aggatgagtt tgtcgtcttg gcctgtgatg
     1261 ggatctggga tgtgatgagc aatgaggagc tctgtgagtt tgttaagtct aggcttgagg
     1321 tgtcggacga cctggagaat gtgtgcaatt gggtagtgga cacttgttta cataagggaa
     1381 gtcgagataa catgagtgtt gtattagttt gcttttcaaa tgcccccaag gtctcagagg
     1441 aagccgtgaa gagagattca gagttggata agcacttgga atcacgggtt gaagaaatca
     1501 tgcagaagtc tggggaggaa ggaatgcctg atcttgccca tgtgatgcgc attttgtctg
     1561 cagaaaatat cccgaattta cctcccgggg gaggtctcgc tggcaagcgc catgttattg
     1621 aagctgttta tagtagactt aatccacaca aagacaatga tggggcctcc gatgaagcag
     1681 aggaaggtgg atcacagggg aaattggtgg aagctctcag gcaaatgaga gttaaccata
     1741 ggggaaacta ccgacagctg ctggaggaga tgctgactag ttacaggcta gctaaagtag
     1801 agggagaaga gagcccggct gacccagcgg ctgcagctgc ttcttcgaac agtgatggtg
     1861 gaaacccagt ggcaatgcag gagagagaca ctgagggtgg tcctgctggc ctagacagcc
     1921 gtaatgaaga tgcagggaca aagaggagtg ctgaaaatat atgacctttt ttgagtaata
     1981 tctttgtgat cttgatttga cagtttttac ttaggaagta tactgtgtgc ttttatataa
     2041 ccattttgtt atttgaatct tgaaaaacta gttttcttat attcctatag tcttggtttt
     2101 tttaaaggcc tttgcataca cctttatgat agtttaagat tgactattta tagtactttg
     2161 aatttaatga acttatatgt catttcacat tgtatgcaga aaacaggttg tcgttattaa
     2221 actcagtagt ttaattctta gtagttaggg ttagagatta ctggttggaa agacaactat
     2281 acattgtgag aagaaacctt gttgtgtaga attgttgtgt agaattgaca ctgcttattt
     2341 tccatcctca tacctttgca tagtcagctt tgtaatccat ttgtgtctac ttactgtttt
     2401 agttcttcca aggatggcta gagctggcct ataactttct tttagagttg ataatctgtc
     2461 tgttttaatt tgtttatatt ttacatttct gtagttttat ttttagaatt tattgagatg
     2521 tgtggttatc tttcctttct ctgtattctt tatgaaaatt tttaaagaac tctgtacttt
     2581 tcctacagct ttcatttgta tattctgtat agcctattta cacatcacaa tgtatgtctc
     2641 ccgcgtgctt agactggaac gatgcgtgta gtaaataaag cctgtgctgt ggcctcgcct
     2701 gcgcatttgt actctctcag tttccactta tttcacagtc agctttgggg cactggaaaa
     2761 ctcaataaag gtcatttatg tcctggtaaa tttggcacac tttggctcag ctgtttttta
     2821 tcaacaaaag agaaaacatt ttaattaaaa gtatatatta aagtcttgct tactcaaaaa
//
