LOCUS       NM_001405458            5883 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus diphosphoinositol pentakisphosphate kinase 2
            (Ppip5k2), transcript variant 1, mRNA.
ACCESSION   NM_001405458 XM_006529489
VERSION     NM_001405458.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Akoto,T., Hadvina,R., Jones,S., Cai,J., Yu,H., McCord,H.,
            Jin,C.X.J., Estes,A.J., Gan,L., Kuo,A., Smith,S.B. and Liu,Y.
  TITLE     Identification of Keratoconus-Related Phenotypes in Three Ppip5k2
            Mouse Models
  JOURNAL   Invest Ophthalmol Vis Sci 65 (6), 22 (2024)
   PUBMED   38869368
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5883)
  AUTHORS   Du,C., Wang,X., Wu,Y., Liao,W., Xiong,J., Zhu,Y., Liu,C., Han,W.,
            Wang,Y., Han,S., Chen,S., Xu,Y., Wang,S., Wang,F., Yang,K., Zhao,J.
            and Wang,J.
  TITLE     Renal Klotho and inorganic phosphate are extrinsic factors that
            antagonistically regulate hematopoietic stem cell maintenance
  JOURNAL   Cell Rep 38 (7), 110392 (2022)
   PUBMED   35172146
REFERENCE   4  (bases 1 to 5883)
  AUTHORS   Khaled,M.L., Bykhovskaya,Y., Gu,C., Liu,A., Drewry,M.D., Chen,Z.,
            Mysona,B.A., Parker,E., McNabb,R.P., Yu,H., Lu,X., Wang,J., Li,X.,
            Al-Muammar,A., Rotter,J.I., Porter,L.F., Estes,A., Watsky,M.A.,
            Smith,S.B., Xu,H., Abu-Amero,K.K., Kuo,A., Shears,S.B.,
            Rabinowitz,Y.S. and Liu,Y.
  TITLE     PPIP5K2 and PCSK1 are Candidate Genetic Contributors to Familial
            Keratoconus
  JOURNAL   Sci Rep 9 (1), 19406 (2019)
   PUBMED   31852976
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5883)
  AUTHORS   Yousaf,R., Gu,C., Ahmed,Z.M., Khan,S.N., Friedman,T.B.,
            Riazuddin,S., Shears,S.B. and Riazuddin,S.
  TITLE     Mutations in Diphosphoinositol-Pentakisphosphate Kinase PPIP5K2 are
            associated with hearing loss in human and mouse
  JOURNAL   PLoS Genet 14 (3), e1007297 (2018)
   PUBMED   29590114
  REMARK    GeneRIF: demonstration that PPIP5K2 has a role in hearing in humans
            indicates that PP-IP signaling is important to hair cell
            maintenance and function within inner ear
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5883)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 5883)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   8  (bases 1 to 5883)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 5883)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5883)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102191.11, AC162298.4 and AC099860.13.
            
            On Apr 25, 2022 this sequence version replaced XM_006529489.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.697.1,
                                           SRR9219382.28917.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AC102191.11        145451-145646
            197-593             AC102191.11        153680-154076
            594-789             AC102191.11        156137-156332
            790-880             AC102191.11        158567-158657
            881-966             AC102191.11        159530-159615
            967-1121            AC102191.11        160292-160446
            1122-1223           AC102191.11        162696-162797
            1224-1385           AC102191.11        164773-164934
            1386-1507           AC102191.11        165838-165959
            1508-1609           AC102191.11        167853-167954
            1610-1696           AC102191.11        168997-169083
            1697-1772           AC102191.11        170031-170106
            1773-1882           AC102191.11        170229-170338
            1883-1968           AC102191.11        171359-171444
            1969-2094           AC162298.4         89239-89364         c
            2095-2216           AC162298.4         88993-89114         c
            2217-2399           AC162298.4         88631-88813         c
            2400-2541           AC162298.4         83136-83277         c
            2542-2765           AC162298.4         82055-82278         c
            2766-2893           AC162298.4         78080-78207         c
            2894-3008           AC162298.4         77087-77201         c
            3009-3116           AC162298.4         75564-75671         c
            3117-3233           AC162298.4         75358-75474         c
            3234-3398           AC162298.4         71839-72003         c
            3399-3572           AC162298.4         69275-69448         c
            3573-3645           AC162298.4         68003-68075         c
            3646-3765           AC162298.4         65611-65730         c
            3766-3828           AC162298.4         64711-64773         c
            3829-3969           AC162298.4         61173-61313         c
            3970-4095           AC099860.13        229263-229388       c
            4096-5883           AC099860.13        223583-225370       c
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 47.76 cM"
     gene            1..5883
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /note="diphosphoinositol pentakisphosphate kinase 2"
                     /db_xref="GeneID:227399"
                     /db_xref="MGI:MGI:2142810"
     exon            1..196
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            197..593
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     CDS             462..4208
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /EC_number="2.7.4.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     inositol hexakisphosphate and
                     diphosphoinositol-pentakisphosphate kinase 2; VIP1 homolog
                     2; insP6 and PP-IP5 kinase 2; histidine acid phosphatase
                     domain-containing protein 1; histidine acid phosphatase
                     domain containing 1"
                     /codon_start=1
                     /product="inositol hexakisphosphate and
                     diphosphoinositol-pentakisphosphate kinase 2 isoform 1"
                     /protein_id="NP_001392387.1"
                     /db_xref="GeneID:227399"
                     /db_xref="MGI:MGI:2142810"
                     /translation="MSNSRKMSEPPRFFVGPEDAEINPGNYRRFFHHAEEEEEEEDES
                     PPERQIVVGICSMAKKSKSKPMKEILERISLFKYITVVVFEEEIILNEPVENWPLCDC
                     LISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSILQAEGILLPRYAIL
                     NRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLF
                     RKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGK
                     VERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFV
                     KNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAV
                     IRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMEL
                     GQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREE
                     PSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDL
                     KIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQR
                     VKARLHEILQKDRDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQ
                     IRYRMEDPKSADIQLYHSETLELMLRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDV
                     QHNGSLKLENTMELYRLSKALADIVIPQEYGITKAEKLEIAKGYCTPLVRKIRSDLQR
                     TQDDDTVNKLHPVYSRGVLSPERHVRTRLYFTSESHVHSLLSILRYGALCDDSKDEQW
                     KRAMDYLNVVNELNYMTQIVIMLYEDPNKDLSSEERFHVELHFSPGAKGCEEDKNLPS
                     GYGYRPASRENEGRRSLKTDDDEPHTSKRDEVDRAVMLFKPLVSEPIHIHRKSPLPRS
                     RKITANEEETPLSVSSPEGTGTWLHYTSGVGTGRRRRRSGEQITSSPVSPKSLAFTSS
                     IFGSWQQVVSENANYLRTPRNLVEQKQNPTVGSHCAGLFSTSVLGGSSSAPNLQDYAR
                     THRKKLTSSGCIDDATRGSAVKRFSISFARHPTNGFELYSMVPSICPLETLHNALFLK
                     QVDDFLASIASPSTEVLRKVPEMSSMATRSSPGMRRKISLNTYTPTKILPTPPAALKS
                     SKASSKAAAGGPSQAMAPHTSSRKKSINSKTEGHEPKKSTGKKR"
     exon            594..789
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            790..880
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            881..966
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1121
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1223
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1385
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1507
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1609
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1696
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1772
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1882
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1968
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2094
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2216
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2399
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2541
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2765
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2893
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3008
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3116
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3233
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3398
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3572
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3573..3645
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3646..3765
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3766..3828
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..3969
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            3970..4095
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     exon            4096..5883
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5744..5749
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /note="hexamer: ATTAAA"
     regulatory      5761..5766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /note="hexamer: AATAAG"
     polyA_site      5767
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /note="major polyA site"
     polyA_site      5777
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
     regulatory      5859..5864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
                     /note="hexamer: AATAAA"
     polyA_site      5883
                     /gene="Ppip5k2"
                     /gene_synonym="Cfap160; D330021B20; Hisppd1; mKIAA0433;
                     Vip2"
ORIGIN      
        1 gtgtcggagg ctcctgaggt gtgccagtgc cagcggttcc gcggtggccg gcgagggaag
       61 cttcaggtcc acgcctccga gagccgcctc ggcacaagcg tccttccctc gcccccgcaa
      121 ttaaaggggg aggaaccgaa acctggatgg gctcaagcat ctcccgcaga tcgcttgctg
      181 cccgcagcca cggcaggtaa aaggaggata tctgttccaa cccgggcaag cagcagcctc
      241 attgtccttg cgttttaaac tacaacagca attgggagtt caacaatgaa gcaatgatat
      301 ctgagaacaa aggaacactg gccaactaaa tgtcagcaca tttcatgcga ttgggtgaaa
      361 acactgcagc agacctgggt gtcagctggc agcctgagaa tgtgctcttc tgacaccatt
      421 tatctgaagc agctttagta tagactattt aaactttatg tatgtcaaat tcaagaaaaa
      481 tgagtgaacc ccccagattc tttgttgggc ccgaagatgc ggaaatcaat cctggaaact
      541 atcgtcgctt cttccaccat gctgaagagg aggaggagga ggaggatgag tctccaccag
      601 aaaggcagat tgtggttgga atatgttcca tggcaaagaa atccaaatcc aaacccatga
      661 aggaaattct ggaacggatc tccttgttta agtacatcac agtagtggtt tttgaagagg
      721 aaattatttt gaatgaaccc gtggaaaact ggcctttatg tgattgtctt atttctttcc
      781 attctaaagg atttccactg gacaaagcag ttgcctatgc aaaactcagg aatccatttg
      841 taatcaatga cttgaatatg cagtatctca tacaagatag aagagacgtg tatagcattc
      901 ttcaagctga aggcatccta cttcctcggt atgcaatcct gaaccgtgat ccaaataacc
      961 cgaaagaatg caacctgatt gaaggagaag atcatgtaga agtgaatggg gaagttttcc
     1021 aaaagccctt tgtggagaag ccggtcagtg cagaggacca caatgtttac atttattatc
     1081 caacatccgc cggaggtggg agtcagagac ttttccggaa gattggcagt agaagtagtg
     1141 tgtattcccc agagagcaac gttcgaaaaa cggggtcata tatatatgaa gagtttatgc
     1201 ctacagatgg tactgatgtt aaggtttata ccgtgggtcc agactatgca catgctgaag
     1261 ctcgaaaatc tccagctctc gatggcaagg tagagcgaga cagcgaaggg aaggaagtaa
     1321 gataccccgt catcctcaat gcacgggaga agctgattgc gtggaaagtc tgccttgctt
     1381 ttaagcaaac ggtttgtggc tttgatttgt tacgtgccaa tggacaatcc tatgtatgcg
     1441 atgtcaatgg cttcagtttt gtgaaaaatt ccatgaagta ctatgatgac tgtgcaaaaa
     1501 ttcttggcaa tatcgtaatg cgggagcttg caccacagtt tcatatcccg tggtcaatac
     1561 cattagaagc tgaagatatt ccaattgtac caactacctc tggaactatg atggaacttc
     1621 gatgtgtcat cgctgtgata cgacatgggg atcgaacacc gaaacagaaa atgaaaatgg
     1681 aagtgaggca tcagaagttt tttgaccttt ttgaaaagtg tgatggatat aaatcaggga
     1741 agttaaagct caagaagcca aaacaattac aggaagtcct ggatattgca aggcagcttc
     1801 ttatggagct ggggcaaaat aatgactctg aaattgaaga aaacaagtca aaacttgaac
     1861 aacttaagac tgtgttagag atgtatggtc atttttcagg aataaaccgt aaggtccaat
     1921 taacctatct tcctcatggc tgtccgaaaa catccagtga ggaggaagat aaccgaagag
     1981 aggagccgtc cttacttttg gttctaaaat ggggaggaga actgacccct gcgggcaggg
     2041 ttcaggctga agagctggga agagcttttc ggtgtatgta ccctggaggt caaggagatt
     2101 atgcaggatt tcctggttgt ggtttactcc gactacatag cacctacagg catgacctca
     2161 aaatatatgc ctctgatgaa gggcgggtcc agatgacggc ggctgctttc gcaaagggtc
     2221 tcttggctct agaaggagag cttaccccca ttcttgttca gatggtgaaa agtgcaaaca
     2281 tgaacggcct tttggatagt gatagtgact ctttgagtag ctgtcagcag cgtgtgaaag
     2341 cgaggcttca tgaaatactt cagaaagaca gggatttcac agctgaggac tacgagaagc
     2401 ttactccatc tggaagcatt tctgttatca aatcaatgca tttgattaaa aatccagtga
     2461 aaacctgtga caaagtgtat tccttgattc aaagtttgac ttcccaaatt agatatcgga
     2521 tggaagatcc caaatcagct gatattcagc tttaccacag tgagaccctg gaacttatgc
     2581 tgcgtagatg gtccaagttg gagaaggatt ttaaaacaaa gaatggaaga tatgatatta
     2641 gcaagatccc tgacatatat gactgcatca aatatgacgt tcagcataac ggttccttga
     2701 agttagaaaa cacaatggaa ctatataggc tttcaaaggc attagcagat attgttatcc
     2761 ctcaggaata tggtataact aaagctgaaa aactggagat tgccaaaggc tactgtactc
     2821 cactagttag aaaaatccga tcagatcttc agaggacaca agatgatgat actgtcaata
     2881 aactccatcc tgtgtattct agaggtgttc tgtctcctga gcggcacgtc cgcaccagat
     2941 tatattttac cagtgagagt catgtgcatt ctttactatc cattcttcgg tatggcgcct
     3001 tatgcgatga ctcaaaggat gagcagtgga aacgagctat ggattatcta aacgtcgtca
     3061 atgagctcaa ctacatgacg cagattgtta tcatgctcta tgaggatccc aacaaggacc
     3121 tttcctcaga ggaacgcttt catgttgagt tacattttag tcctggagcc aaaggctgtg
     3181 aagaagataa gaatttgcca tctggctatg ggtataggcc agcatccagg gagaatgaag
     3241 gcagaaggtc tcttaaaact gacgatgatg agcctcacac ttctaaacga gatgaagttg
     3301 accgggccgt gatgttgttt aaaccattgg tgtctgagcc aattcatata cacaggaaat
     3361 caccgcttcc gaggtccagg aagattactg caaatgagga agagaccccc ctgagtgtgt
     3421 ctagcccaga gggcactggc acctggctgc attataccag tggtgtgggt actgggcgtc
     3481 gaagacgcag atcaggggaa caaatcacct cttcccctgt ctcccccaaa tcattggctt
     3541 tcacatccag tatttttggc tcatggcaac aggttgtgtc tgagaatgcc aactatctcc
     3601 gaacaccaag aaatcttgtg gaacagaagc agaaccctac agtagggtct cactgtgcgg
     3661 gcctgtttag cacctcagtg ctcgggggtt cttcaagtgc acctaaccta caggattatg
     3721 cccgtactca tcgtaaaaag ctgacttctt ctggctgcat agatgacgcc acacgcgggt
     3781 ctgctgttaa aaggttttct atctcatttg ctcgacaccc aaccaatggc ttcgagctgt
     3841 attccatggt accatccatc tgtcctctag aaacacttca caatgccctg tttttaaagc
     3901 aagtggatga ctttctggct tccattgctt ctccatcaac ggaggttctg cgcaaggtcc
     3961 ccgaaatgtc ctctatggcc acacgttcca gtccaggaat gagaagaaaa atttccttaa
     4021 atacgtatac acctacaaag atcctcccaa caccacctgc tgctttaaag agcagcaaag
     4081 caagcagcaa agcagcagcc ggcggaccat ctcaggcaat ggctccccat acctcatctc
     4141 ggaagaagag cataaatagc aaaaccgagg ggcatgaacc caagaaaagc actgggaaga
     4201 aaagatgagc acacaccaga accgaaagtt tccatgctta taaagggact tgttgcttag
     4261 caaaaagaat gtctaagaaa ccttgactga agctggtcag agccagtgat tcatgtgtta
     4321 tcatgcttta ctttatacat ctgtgtattt ctttaaacac ctcgaaagct agaggacctc
     4381 ctgtttttat tcaaacaagg agctctggca ggatctagaa atgttcaaaa gaatgtctgt
     4441 ccgctttgtg taaggaaatt catgacaact gctattgctg tcactgtgat gattcccatg
     4501 tgcctgtggc agagacaaag gacacaggaa cccggctgac tggtaccaaa gtgcactctc
     4561 ttcaggtgcc tctacacaca tgcatttcta cagaattaca cttagcgagt gggattggac
     4621 aggcaaagat catgtgttga tgactttttt taaaaagtac atttccaaag agatctcgtt
     4681 gaccatttac attacaatgt ttttgtcatt gctacagtaa catatgctgc aatatttatt
     4741 gactggatta tatagaccat gaattatttt ttcaatttgt gctttgaatt ttttattgtg
     4801 aattatttaa aatattacaa atgcaggtgg gagaactata cctttataca cataaattta
     4861 caagttaatc tgtagaaaat attttcttta tacatatctt taccatcaag aaaaatttct
     4921 tggttcacca agaaaaggtt tttttttttt tttgtattag caagagaggc aattttttag
     4981 agtttctttt tatgaactgt aactgctaaa tttgtaaatc ttacacgtga ttttctgagt
     5041 ttttcttgag acctatggac tcaggtctat gatgcgagtt ctgctgttca taagtgtcat
     5101 ccagggtata gttcttgtgt ttccattctt atgtccagtg tatttgatga tttcaccaag
     5161 aaaactcaaa agattgaaca tttgtagtaa gtaaatggtg tttacaaatg atgagatttg
     5221 gaagaaagat ggagatgact attgtaattt cgtctaatat ttaaataaaa ggacatcatt
     5281 ctccttggtg ctctttctcc ctgatgcaca agtaattgtt tgaaatattt taaattactc
     5341 acaaactaaa caacctgcta aataaaaatc tcaaacattg gctataatgc aacttaaggt
     5401 agtaagctgt agcttcaagc ttatgtttag aagtactgga cccttattgt atagttagtg
     5461 taattcttca aaagagactt ctaaccttaa ataatcttac atgaactgac ttctagagaa
     5521 taggcgtatt atattatctt ggttgtttaa aaagggatga gatacagaag ctccaaatat
     5581 atacaaataa ataagtacat atatacttca cttgaatgta aatggattat atggtggtaa
     5641 atcttgtaaa caatcgaatg tatgtatgtt ctctgtgtgt gaatatgtga ttaatgtact
     5701 catggggggt gaggagacta cttttatttt ttaagtggaa tgaattaaaa ttctttttag
     5761 aataagagtg acttgaattt aaagcatttt atttgtgttc tttcttttat gttttgaaat
     5821 agaaaatccc cagcagcatt ttacattaag tactattcaa taaaactcat gagtgagcat
     5881 aca
//
