LOCUS       NM_001195086            5259 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus protein tyrosine phosphatase, receptor type, f
            polypeptide (PTPRF), interacting protein (liprin), alpha 1
            (Ppfia1), transcript variant A, mRNA.
ACCESSION   NM_001195086
VERSION     NM_001195086.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Emperador-Melero,J., Andersen,J.W., Metzbower,S.R., Levy,A.D.,
            Dharmasri,P.A., de Nola,G., Blanpied,T.A. and Kaeser,P.S.
  TITLE     Distinct active zone protein machineries mediate Ca2+ channel
            clustering and vesicle priming at hippocampal synapses
  JOURNAL   Nat Neurosci 27 (9), 1680-1694 (2024)
   PUBMED   39160372
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5259)
  AUTHORS   Bernadzki,K.M., Gawor,M., Pezinski,M., Mazurek,P., Niewiadomski,P.,
            Redowicz,M.J. and Proszynski,T.J.
  TITLE     Liprin-alpha-1 is a novel component of the murine neuromuscular
            junction and is involved in the organization of the postsynaptic
            machinery
  JOURNAL   Sci Rep 7 (1), 9116 (2017)
   PUBMED   28831123
  REMARK    GeneRIF: we found that liprin-alpha-1 plays an important role in
            AChR clustering and the organization of cortical microtubules. Our
            studies provide novel insights into the function of liprin-alpha-1
            at vertebrate neuromuscular synapses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5259)
  AUTHORS   Huang,H., Lin,X., Liang,Z., Zhao,T., Du,S., Loy,M.M.T., Lai,K.O.,
            Fu,A.K.Y. and Ip,N.Y.
  TITLE     Cdk5-dependent phosphorylation of liprinalpha1 mediates neuronal
            activity-dependent synapse development
  JOURNAL   Proc Natl Acad Sci U S A 114 (33), E6992-E7001 (2017)
   PUBMED   28760951
  REMARK    GeneRIF: findings collectively demonstrate that the Cdk5-dependent
            phosphorylation of liprinalpha1 is important for the postsynaptic
            organization during activity-dependent synapse development.
            Erratum:[Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9425-E9426.
            doi: 10.1073/pnas.1717049114. PMID: 29078422]
REFERENCE   5  (bases 1 to 5259)
  AUTHORS   Zurner,M. and Schoch,S.
  TITLE     The mouse and human Liprin-alpha family of scaffolding proteins:
            genomic organization, expression profiling and regulation by
            alternative splicing
  JOURNAL   Genomics 93 (3), 243-253 (2009)
   PUBMED   19013515
REFERENCE   6  (bases 1 to 5259)
  AUTHORS   Schoch,S., Mittelstaedt,T., Kaeser,P.S., Padgett,D., Feldmann,N.,
            Chevaleyre,V., Castillo,P.E., Hammer,R.E., Han,W., Schmitz,F.,
            Lin,W. and Sudhof,T.C.
  TITLE     Redundant functions of RIM1alpha and RIM2alpha in Ca(2+)-triggered
            neurotransmitter release
  JOURNAL   EMBO J 25 (24), 5852-5863 (2006)
   PUBMED   17124501
REFERENCE   7  (bases 1 to 5259)
  AUTHORS   Olsen,O., Moore,K.A., Fukata,M., Kazuta,T., Trinidad,J.C.,
            Kauer,F.W., Streuli,M., Misawa,H., Burlingame,A.L., Nicoll,R.A. and
            Bredt,D.S.
  TITLE     Neurotransmitter release regulated by a MALS-liprin-alpha
            presynaptic complex
  JOURNAL   J Cell Biol 170 (7), 1127-1134 (2005)
   PUBMED   16186258
REFERENCE   8  (bases 1 to 5259)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of mouse Ppfia1 gene in silico
  JOURNAL   Int J Mol Med 12 (2), 263-267 (2003)
   PUBMED   12851729
REFERENCE   9  (bases 1 to 5259)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5259)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153792.4 and AC140268.2.
            
            Transcript Variant: This variant (A) represents the longer
            transcript and encodes the longer isoform (A).
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC153792.4         66901-67111         c
            212-475             AC153792.4         65652-65915         c
            476-577             AC140268.2         26425-26526
            578-742             AC140268.2         26697-26861
            743-817             AC140268.2         27436-27510
            818-919             AC140268.2         27840-27941
            920-1138            AC140268.2         28119-28337
            1139-1285           AC140268.2         29740-29886
            1286-1420           AC140268.2         31366-31500
            1421-1495           AC140268.2         32540-32614
            1496-1579           AC140268.2         32962-33045
            1580-1711           AC140268.2         34109-34240
            1712-1774           AC140268.2         36550-36612
            1775-1854           AC140268.2         37125-37204
            1855-1990           AC140268.2         39222-39357
            1991-2214           AC140268.2         41192-41415
            2215-2446           AC140268.2         42352-42583
            2447-2476           AC140268.2         44590-44619
            2477-2628           AC140268.2         47083-47234
            2629-2801           AC140268.2         48607-48779
            2802-2895           AC140268.2         49096-49189
            2896-2977           AC140268.2         55663-55744
            2978-3178           AC140268.2         55834-56034
            3179-3241           AC140268.2         58227-58289
            3242-3268           AC140268.2         58769-58795
            3269-3444           AC140268.2         62278-62453
            3445-3542           AC140268.2         62545-62642
            3543-3718           AC140268.2         65051-65226
            3719-3787           AC140268.2         65707-65775
            3788-3953           AC140268.2         66236-66401
            3954-4024           AC140268.2         68389-68459
            4025-5259           AC140268.2         69497-70731
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.85 cM"
     gene            1..5259
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /note="protein tyrosine phosphatase, receptor type, f
                     polypeptide (PTPRF), interacting protein (liprin), alpha
                     1"
                     /db_xref="GeneID:233977"
                     /db_xref="MGI:MGI:1924750"
     exon            1..211
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..4012
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /note="isoform A is encoded by transcript variant A;
                     liprin; Liprin-alpha1"
                     /codon_start=1
                     /product="liprin-alpha-1 isoform A"
                     /protein_id="NP_001182015.1"
                     /db_xref="CCDS:CCDS59738.1"
                     /db_xref="GeneID:233977"
                     /db_xref="MGI:MGI:1924750"
                     /translation="MMCEVMPTISEAEGPPGGGGSHGSGSPSQPDADSHFEQLMVSML
                     EERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCRE
                     QLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEV
                     LKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTD
                     GLLDGNHEQESAPSTNGKRSSDGSLSHEDLAKVLELQEVIDRQAREQSQMKERLASLS
                     SHAAELEEDLDTARKDLIKSEEMNTKLQREVREAMAQKEDMEERITTLEKRYLAAQRE
                     ATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEA
                     ELAQRVAALSKSGPLSSGSSAAKEAKLLELTSKLRKAEERHGNIEERLRQMEAQLEEK
                     NQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVE
                     NAKKQLEETQHDKDQLVVTIEALKAELEQMRLRGPSLHHGRPHLGSVPDFRFSVADGH
                     VDAYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDV
                     SDGEDDRDTLLSSVDLLSPSGQADAQTLAMMLQEQLDAINKEIRLIQEEKENTEQRAE
                     EIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRL
                     GVMTLPSDLRKHRRQLPASREEVRDDKTTIKCETSPPSSPRPLRLDRMHKGAPHTVSH
                     EDIRDLRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGPL
                     GKESPGQVGVSETENSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDG
                     PTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAI
                     QEIMSLTSPSAPPTSRTTTGNVWLTHEEMETLTATPQTEDEEGSWAQTLAYGDMNHEW
                     IGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCL
                     RRLNYDRKELERKREESQNETRDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALL
                     ALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLITGTDRRFDEDDDKSFRRAPSW
                     RKKFRPKDIRGLASGSAETLPANFRVTSSMSSPSMQPKKVQMDGSVSGAQRLDSATVR
                     TYSC"
     exon            212..475
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            476..577
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..742
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            743..817
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            818..919
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1138
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1285
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1420
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1495
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1579
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1711
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1774
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1854
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1990
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2214
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2446
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2476
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2628
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2801
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2895
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..2977
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3178
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3179..3241
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3268
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..3444
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3542
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3543..3718
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3719..3787
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3788..3953
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4024
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4025..5259
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5228..5233
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /note="hexamer: AATAAA"
     polyA_site      5256
                     /gene="Ppfia1"
                     /gene_synonym="C030014K08Rik; LIP.1; LIP1"
                     /note="major polyA site"
ORIGIN      
        1 ggacgtcggg cacgttgacg ccggcgacgc gccgggcccg gttctctgcg ctcgctagtg
       61 tcccgcggcg gcgggccggc cttcgtgacg agcggggcgg cgggcccggg ggccgggcga
      121 ggacgcagcc gcggggctgc gtgggtcggc ccgcagccgg ccgccgcccg aaggccgctt
      181 catagccccg gagccgcgcc cgccggccaa gatgatgtgc gaggtgatgc cgaccattag
      241 tgaagcagaa ggccctccag gaggaggtgg gagccatggg tcaggctccc cttcacagcc
      301 agatgctgat tcgcattttg agcagctcat ggtttccatg ttggaagaac gggaccgcct
      361 tctggacaca ctaagagaga cacaagaaac actggcatta acccagggga agttgcatga
      421 agttggtcat gaaagagatt ctttgcagag gcagctcaat actgcacttc cacaggagtt
      481 tgctgcgctc acgaaggaac tcaatgtgtg cagggagcag cttctagaga gagaggaaga
      541 aatcgccgag ctgaaggcag agaggaacaa cactaggctg ctgttggagc acctagagtg
      601 ccttgtctcc aggcatgagc gctctctccg gatgacagtg gtaaagaggc aggcacagtc
      661 acctgcaggc gtgtccagtg aggtggaagt gctgaaagcc ctgaagtcac tgtttgaaca
      721 ccacaaagcc ttggatgaga aggtacgaga gcgactacga gtggctctgg agaggtgcag
      781 cttgttagaa gaggagctgg gtgccaccca caaagagcta atgattctta aagaacagaa
      841 taatcagaag aaaacactaa cagatggact gcttgacgga aaccatgaac aggaaagtgc
      901 accaagcacc aacggcaaga gatcttctga tggctccttg agtcatgagg atcttgccaa
      961 agtgctggag ctgcaggaag tcatagacag gcaggcgaga gagcagagcc agatgaagga
     1021 gcgcctggcc tccctgtcca gtcatgcagc agaactagaa gaggatctgg acacagctag
     1081 aaaagacctc atcaagtctg aagagatgaa tacgaaactg cagcgagaag tccgtgaagc
     1141 aatggcccag aaggaggata tggaagagag aatcaccacc cttgagaaac gctacctcgc
     1201 cgcacagcgt gaagccacgt ctgtgcatga cctcaatgac aaacttgaaa atgaaattgc
     1261 aaataaagac tccatgcacc gacagacaga agacaagaac cgccagttgc aagagcgtct
     1321 ggagttggcc gagcagaagc tgcagcagac cctgcgcaaa gctgagacgc tgccagaggt
     1381 ggaggctgag ctggcccaga gggtggctgc actctccaag tctggtcctt tgtcttctgg
     1441 gagttcagct gctaaggaag caaaactgtt ggagcttact tccaagctta ggaaggctga
     1501 ggagagacat ggcaatattg aagagaggct gcggcagatg gaggcacagc tggaggagaa
     1561 aaatcaggag ctgcagcggg caaggcagag agaaaagatg aacgaggaac ataataagcg
     1621 cctgtctgac actgtggata agttgctctc agaatccaat gagaggctgc agctgcatct
     1681 gaaggagaga atggcggcgc tggaggacaa gaattctcta ctaagggaag ttgaaaatgc
     1741 aaagaagcaa ctagaagaga cccagcatga taaggaccaa cttgtcgtga ctattgaagc
     1801 cctgaaggct gagctggagc agatgagact gagaggtcct tcactccatc atggccgacc
     1861 ccatttgggc agcgtcccgg acttcaggtt ctctgtggca gacggacatg tggatgctta
     1921 cagcaccagt gcagtgttgc ggcgccccca gaagggccga ctggcagcgc tgcgggatga
     1981 gccctccaag gtgcagaccc tcaatgagca ggactgggaa cgggcccaac aagccagcgt
     2041 cttggcaaat gtggcacagg catttgagag tgatgtcgat gtgtctgatg gtgaagatga
     2101 ccgagataca ctgctcagct cagttgatct gctgtcaccc agtggacagg ctgatgccca
     2161 gacactggcc atgatgcttc aggaacagct ggatgctatc aacaaagaga tcaggctgat
     2221 acaagaagag aaggagaata cagaacagcg ggcagaggag atcgagagca gagtgggcag
     2281 tggaagcttg gacaatcttg gtcgttttag atcaatgagc tccattcccc cctaccctgc
     2341 ttcctcgctt gctggctcct ctcctccagg cagtggtcgg tccacaccaa gaagggtccc
     2401 tcacagtcct gcccgggagg tggacaggct gggtgtcatg actctgccta gtgacttaag
     2461 gaagcaccgg agacagttgc cagcttccag agaagaggta cgagacgaca agacgacgat
     2521 aaaatgtgaa acctctcccc cctcctcccc gaggcccctg cgcttagaca ggatgcacaa
     2581 gggcgcaccg cacacggtca gccatgagga catcagggac ctgaggaact ccacaggctc
     2641 ccaggatggt cccgtgagca accctagcag cagtaatagt agccaggact ccctccacaa
     2701 agccccaaag aagaagggta tcaaatcctc cattggccgc ttgtttggga agaaggaaaa
     2761 gggccggcct ggtccactgg gcaaggagtc accagggcaa gttggtgttt cagagacaga
     2821 aaattcatct caagatgccc tgggactcag caaactggga ggacaagctg agaaaaaccg
     2881 taaacttcag aaaaagcacg agctgctgga ggaggcccgc aggcaaggct tgccttttgc
     2941 acagtgggat gggcccacag tggtagtgtg gttagagctc tgggttggga tgcctgcctg
     3001 gtatgtggct gcttgccgag caaatgtcaa gagtggtgcc atcatgtcag ccctgtcaga
     3061 cactgagatc cagcgggaga ttggcatcag caaccccctg cataggctga agctgcggct
     3121 ggccatccaa gagataatgt cactgactag cccttctgct ccacccacat ccaggacgac
     3181 cacaggaaat gtctggttga cacatgaaga gatggaaacg cttacagcca cgccacaaac
     3241 ggaagacgag gagggaagct gggctcagac tctagcatat ggggacatga accacgagtg
     3301 gatcggcaat gagtggctgc ccagcctggg cctgccgcag taccgcagct actttatgga
     3361 gtgccttgta gatgcccgga tgctggacca cttgaccaag aaagacttgc gagggcagct
     3421 gaagatggtc gacagcttcc acagaaacag tttccagtgt ggaattatgt gcctgagaag
     3481 gctaaattat gaccgaaaag aactggaaag aaaaagagaa gagagtcaga atgagacaag
     3541 agatgtgctt gtgtggagca atgaccgggt gattcgctgg atcctgtcga ttggccttaa
     3601 agagtatgcc aacaacctaa tagagagcgg tgttcacggt gcacttctgg ctttggacga
     3661 gactttcgat ttcagtgccc tggcactctt gttacagatt ccaacacaga atacgcaggc
     3721 tcgagctgtc ttggaaagag aattcaacaa ccttttgatc acggggactg atcggaggtt
     3781 tgatgaggat gatgacaaaa gcttcaggag ggcgccttcc tggaggaaga agttcagacc
     3841 aaaggacatt cgtggcttgg cctctggctc tgcagagacg ttgcctgcca acttccgggt
     3901 cacctcctca atgtcatctc cctctatgca gccaaagaag gttcagatgg acggtagtgt
     3961 gtccggagca cagaggttgg actctgccac agtcaggact tactcctgct agagcctcct
     4021 gctgtttacc cacactactt ctacagatga cgtcgctgag gcacggacgg tgtagagctc
     4081 gtctggcaac ttttgctatg tggacccaag atattttatt acagagtttt taaactagtg
     4141 aagaaaaaaa tcataaatac catagagaaa atattttaga attgactgtt tcttatattt
     4201 atgtaaactt atgcctcctc atttatatag ttacttactc tttcatgtat atccaggcta
     4261 taaatatcct tttgagtcaa ctcatgttct tatacctaat tttagtcttt taaaggaatg
     4321 tactgtaatg cttgtgtata aatctgagca aatatagggc ttttgtaaat tatgcattta
     4381 ctgtaattat tcctgtttaa ttttttaatg atgaacccga cagaggaaag gattttgcta
     4441 cgtttataaa gtcatcatga tacaagccat gtgcattctc ctctacccag ctctgtaaca
     4501 atcattctga aagaagcaga ctttatttcc agatgttttc tgtcatattt taatgactga
     4561 ccttaaccat actttttcta gacaaaatct cagcttccat tagacttgag actgatggtc
     4621 aggactctgt gaacagccct taccccaacc acatccctag ccctgctctg gacatgggtg
     4681 gattgtctcc agaggctgag cagggccctg agctggtaac agcactctac aggggcaggg
     4741 tggcctcacc tgcagactga gcagctgatg gagcctcgaa gccatctccc tccctccaca
     4801 ctcatcaagg accaatcatg gggcaggtgc tcatttcctg gctggggcag agcaggtgaa
     4861 agcaagagac ttcagcggag caggcagcct gcaactccaa taaaggcctt atttttcttg
     4921 tataaactgt cttacattta tttgtaactg ttgtaagagt ggacctagtt ctccactcag
     4981 ctgtcttgct agcctcctga ctgacttcag tgaactaaga agccacaggg ctcaggcaca
     5041 catgtgcgta tttattgtgt agctgatgtg ggagaggctt ttatatttgc tttctgcttt
     5101 taattcatct ggacagttgg ttgttctgtt tttctgttaa tcgtttgtga ccctcccgat
     5161 tatataacag tctgtgcagt caacttctga actgttttta tcacagtgtt atttattgct
     5221 gtttttcaat aaagtcccca gcattttcca ctgccagtt
//
