LOCUS       NM_011147               2483 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus protein phosphatase with EF hand calcium-binding
            domain 1 (Ppef1), mRNA.
ACCESSION   NM_011147 XM_142281 XM_985156 XM_987222
VERSION     NM_011147.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Lavoie-Ouellet,C., Clark,M.E., Ruiz,J., Saindon,A.A. and Leclerc,P.
  TITLE     The protein phosphatase with EF-hand domain 1 is a
            calmodulin-binding protein that interacts with proteins involved in
            sperm capacitation, binding to the zona pellucida, and motility
  JOURNAL   Mol Reprod Dev 88 (4), 302-317 (2021)
   PUBMED   33783058
  REMARK    GeneRIF: The protein phosphatase with EF-hand domain 1 is a
            calmodulin-binding protein that interacts with proteins involved in
            sperm capacitation, binding to the zona pellucida, and motility.
REFERENCE   3  (bases 1 to 2483)
  AUTHORS   Ramulu,P., Kennedy,M., Xiong,W.H., Williams,J., Cowan,M., Blesh,D.,
            Yau,K.W., Hurley,J.B. and Nathans,J.
  TITLE     Normal light response, photoreceptor integrity, and rhodopsin
            dephosphorylation in mice lacking both protein phosphatases with EF
            hands (PPEF-1 and PPEF-2)
  JOURNAL   Mol Cell Biol 21 (24), 8605-8614 (2001)
   PUBMED   11713293
REFERENCE   4  (bases 1 to 2483)
  AUTHORS   Bulfone,A., Gattuso,C., Marchitiello,A., Pardini,C., Boncinelli,E.,
            Borsani,G., Banfi,S. and Ballabio,A.
  TITLE     The embryonic expression pattern of 40 murine cDNAs homologous to
            Drosophila mutant genes (Dres): a comparative and topographic
            approach to predict gene function
  JOURNAL   Hum Mol Genet 7 (13), 1997-2006 (1998)
   PUBMED   9817915
REFERENCE   5  (bases 1 to 2483)
  AUTHORS   Montini,E., Andolfi,G., Caruso,A., Buchner,G., Walpole,S.M.,
            Mariani,M., Consalez,G., Trump,D., Ballabio,A. and Franco,B.
  TITLE     Identification and characterization of a novel serine-threonine
            kinase gene from the Xp22 region
  JOURNAL   Genomics 51 (3), 427-433 (1998)
   PUBMED   9721213
REFERENCE   6  (bases 1 to 2483)
  AUTHORS   Sherman,P.M., Sun,H., Macke,J.P., Williams,J., Smallwood,P.M. and
            Nathans,J.
  TITLE     Identification and characterization of a conserved family of
            protein serine/threonine phosphatases homologous to Drosophila
            retinal degeneration C
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11639-11644 (1997)
   PUBMED   9326663
REFERENCE   7  (bases 1 to 2483)
  AUTHORS   Montini,E., Rugarli,E.I., Van de Vosse,E., Andolfi,G., Mariani,M.,
            Puca,A.A., Consalez,G.G., den Dunnen,J.T., Ballabio,A. and
            Franco,B.
  TITLE     A novel human serine-threonine phosphatase related to the
            Drosophila retinal degeneration C (rdgC) gene is selectively
            expressed in sensory neurons of neural crest origin
  JOURNAL   Hum Mol Genet 6 (7), 1137-1145 (1997)
   PUBMED   9215685
REFERENCE   8  (bases 1 to 2483)
  AUTHORS   Banfi,S., Borsani,G., Bulfone,A. and Ballabio,A.
  TITLE     Drosophila-related expressed sequences
  JOURNAL   Hum Mol Genet 6 (10), 1745-1753 (1997)
   PUBMED   9300667
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL974311.6 and AL732450.5.
            
            On or before Jan 27, 2007 this sequence version replaced
            XM_142281.7, XM_987222.1, XM_985156.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN00849379, SAMN00849381
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AL974311.6         6555-6720           c
            167-294             AL732450.5         139066-139193       c
            295-355             AL732450.5         137075-137135       c
            356-525             AL732450.5         130749-130918       c
            526-640             AL732450.5         122212-122326       c
            641-687             AL732450.5         118157-118203       c
            688-854             AL732450.5         92210-92376         c
            855-891             AL732450.5         88847-88883         c
            892-1041            AL732450.5         87430-87579         c
            1042-1188           AL732450.5         83738-83884         c
            1189-1374           AL732450.5         80587-80772         c
            1375-1517           AL732450.5         78773-78915         c
            1518-1624           AL732450.5         77434-77540         c
            1625-1788           AL732450.5         71256-71419         c
            1789-1873           AL732450.5         70143-70227         c
            1874-2483           AL732450.5         68673-69282         c
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 73.95 cM"
     gene            1..2483
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /note="protein phosphatase with EF hand calcium-binding
                     domain 1"
                     /db_xref="GeneID:237178"
                     /db_xref="MGI:MGI:1097157"
     exon            1..166
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2073
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /EC_number="3.1.3.16"
                     /note="PPEF 1; DRES10; protein phosphatase with EF
                     calcium-binding domain"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase with
                     EF-hands 1"
                     /protein_id="NP_035277.1"
                     /db_xref="CCDS:CCDS41200.1"
                     /db_xref="GeneID:237178"
                     /db_xref="MGI:MGI:1097157"
                     /translation="MGCGTSSKKGNKSKKVIKAALVIQNWYRRYRARLRVRQHYALAI
                     FQSIEYSDEQGQMQLSSFFSFMLENYTKTNNEDSALVTRIFDNTRRESQIKDRDDFLG
                     LIEVPDSYDGPRLQFPLTFTDIHILLQAFKQQQILHAHYVLEVLFEARKVLKQMPNFS
                     HVKTFPAKEITICGDLHGKLDDLMLIFYKNGLPSENNPYVFNGDFVDRGNNSMEILMI
                     LLVCFLVYPSDLHLNRGNHEDFMMNLRYGFTKEILQKYKLHGRKILQVLEEVYTWLPI
                     GTIIDNEILVIHGGISESTDLNTLHQLQRNKMKSVLMPPVLGNQETGEKRNKSASNYV
                     EPRKVEPDKTPSEDLTKQEWEQIVDILWSDPRGKKGCYPNTSRGGGCYFGPDVTSKVL
                     SKNQLKMLIRSHECKPDGYEVSHDGKVITVFSASNYYEEGSNRGAYIRLSYGTMPQFF
                     QYQVTSTSCLNPLHQRMNAMESSAFKILKEKMISRKTDLINAFELRDHSRSGRISLAE
                     WAFSMENILGLNLPWRSLSSHLVTIDSSGSVDYMSSFDDIRIEKPTKDMKSNLTETMY
                     RYRSDLKIIFNIIDSDQSGLISMDEFRTMWKLFNAHYKAHIDDSQIDELASIVDFNKD
                     GNIDFNEFLKAFYVVHKYDKPGTSLA"
     exon            167..294
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..355
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            356..525
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..640
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            641..687
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            688..854
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            855..891
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1041
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1188
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1374
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1517
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1624
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1788
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1873
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2483
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2461..2466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /note="hexamer: AATAAA"
     polyA_site      2483
                     /gene="Ppef1"
                     /gene_synonym="PPEF-1"
                     /note="major polyA site"
ORIGIN      
        1 catagcatct atggactgaa gggaatcagt tggtggtttc ttgaagcagg tagagaggga
       61 cttcacactc tcacttggaa ccagaagttg aatttctaag cataatatgt tttagaagtc
      121 atgggctgtg gcacttcttc aaaaaaaggc aacaaatcta agaaagtgat aaaagccgcg
      181 ttagtcatcc aaaattggta ccggcggtac agagctcgac tgagggtcag acagcattat
      241 gccctcgcca tcttccagtc tattgaatat tctgacgagc aaggccaaat gcagctatcc
      301 agcttctttt cgttcatgtt ggaaaactac acaaagacaa ataatgaaga ttcagcatta
      361 gtaactcgaa tttttgacaa caccaggcgg gaaagccaaa tcaaggatag agatgacttt
      421 ttggggttga tagaagtgcc tgactcctat gatggtcctc ggctgcagtt tcctctgacg
      481 tttactgaca tccatatact tcttcaggcc ttcaagcaac agcagatact tcatgctcac
      541 tacgtcttag aggtgctatt tgaagctagg aaagtcctga agcaaatgcc gaatttctct
      601 catgtaaaaa cctttcccgc caaagagata acaatatgtg gtgatttgca tgggaaactg
      661 gatgatctta tgctgatttt ctataagaat ggcctgcctt cagagaacaa tccatacgtt
      721 ttcaatggtg actttgtaga tcgaggaaac aattccatgg agatcctaat gatcctgctt
      781 gtttgttttc ttgtctaccc ctctgacctg cacctcaata gagggaacca tgaagacttt
      841 atgatgaacc tgaggtatgg cttcacaaaa gaaattttac aaaaatacaa gctacacggg
      901 agaaaaatct tacaagtctt ggaagaagtc tatacctggc tcccaatcgg tacaattatt
      961 gacaacgaaa tcctggtcat acatggtggg atatcagaat ccacagactt gaatacactc
     1021 caccagttac aaagaaataa aatgaaatct gtgttgatgc cgccagtgtt gggaaatcaa
     1081 gagactggcg aaaagagaaa taaatcagcg tcaaattatg ttgaaccaag gaaggttgaa
     1141 ccagacaaga ctccttctga agacttgaca aagcaagaat gggaacaaat agttgacatt
     1201 ctgtggagtg accccagagg caaaaaaggc tgttatccaa acacaagccg aggagggggc
     1261 tgctattttg gaccagatgt tacctctaag gttcttagta aaaaccagtt gaagatgctc
     1321 attaggtctc atgagtgtaa acccgacggg tatgaagtct ctcatgatgg aaaggtcatt
     1381 actgtatttt ctgcttctaa ttattatgaa gaaggcagca accgaggagc ttacatcaga
     1441 ctaagttatg gtacaatgcc acaatttttc cagtaccaag taactagtac atcatgcctg
     1501 aaccctcttc accaaaggat gaatgctatg gaatccagtg ctttcaagat actaaaggaa
     1561 aaaatgattt cacgaaaaac tgatctcatc aacgctttcg agctccgtga ccacagtagg
     1621 tcaggaagga tttcattggc cgagtgggca ttttctatgg agaacatttt ggggctgaac
     1681 ttaccatgga gatctctaag ttcacacctg gtaaccattg attccagcgg aagtgttgac
     1741 tacatgtcca gctttgacga tatccgtatt gaaaagccta cgaaagatat gaagtccaat
     1801 ctaactgaaa ctatgtacag ataccgatct gacctgaaaa tcatatttaa tatcatcgac
     1861 agtgatcaat caggtctgat ctccatggat gaatttcgga ccatgtggaa acttttcaat
     1921 gctcattaca aagctcatat agatgattcc caaattgatg agcttgccag catagtggac
     1981 ttcaacaaag atggcaacat cgactttaat gagtttttaa aggctttcta tgtagtacat
     2041 aaatatgata agccagggac cagtcttgct taagatgaac aaaaacttct cccagcctcc
     2101 ttgaaaacag atgaccccaa gtttcagtct taacaaaggc cccttgaaat tcgtagtagt
     2161 agagaaaagt agaccctaat tcacaccata agcagatgca tcatgtgagt gtttgcagtc
     2221 cttagcaaac tgctattggt aaaactatgt taaataacag ttgatgagat ttacttccag
     2281 tgttaggatt gccagtgata aactgggttt ggacctagta ttggtctcaa atgataccta
     2341 accaggagat caaagaaatt tagaaacata ctgaaaggaa cccagagatc aggagagaaa
     2401 acaattaggg gtaataactg tgagtggctg gggatgaggt gaatactaaa ctattattga
     2461 aataaaatac ttccatcatc aaa
//
