LOCUS       NM_026494               1460 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus phosphopantothenoylcysteine synthetase (Ppcs),
            transcript variant 1, mRNA.
ACCESSION   NM_026494 XM_892697 XM_900258 XM_909105 XM_921155
VERSION     NM_026494.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Mostert,K.J., Sharma,N., van der Zwaag,M., Staats,R., Koekemoer,L.,
            Anand,R., Sibon,O.C.M. and Strauss,E.
  TITLE     The Coenzyme A Level Modulator Hopantenate (HoPan) Inhibits
            Phosphopantotenoylcysteine Synthetase Activity
  JOURNAL   ACS Chem Biol 16 (11), 2401-2414 (2021)
   PUBMED   34582681
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Iuso,A., Wiersma,M., Schuller,H.J., Pode-Shakked,B.,
            Marek-Yagel,D., Grigat,M., Schwarzmayr,T., Berutti,R., Alhaddad,B.,
            Kanon,B., Grzeschik,N.A., Okun,J.G., Perles,Z., Salem,Y., Barel,O.,
            Vardi,A., Rubinshtein,M., Tirosh,T., Dubnov-Raz,G., Messias,A.C.,
            Terrile,C., Barshack,I., Volkov,A., Avivi,C., Eyal,E.,
            Mastantuono,E., Kumbar,M., Abudi,S., Braunisch,M., Strom,T.M.,
            Meitinger,T., Hoffmann,G.F., Prokisch,H., Haack,T.B.,
            Brundel,B.J.J.M., Haas,D., Sibon,O.C.M. and Anikster,Y.
  TITLE     Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase,
            Cause Autosomal-Recessive Dilated Cardiomyopathy
  JOURNAL   Am J Hum Genet 102 (6), 1018-1030 (2018)
   PUBMED   29754768
REFERENCE   3  (bases 1 to 1460)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY754420.1, BX470216.11, CF174441.1, AK006290.1, BM227018.2 and
            BY634907.1.
            
            On Sep 13, 2017 this sequence version replaced NM_026494.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159952.1, BC021894.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 BY754420.1         1-3
            4-4                 BX470216.11        135159-135159       c
            5-513               BY754420.1         5-513
            514-762             CF174441.1         450-698
            763-1351            AK006290.1         275-863
            1352-1395           BM227018.2         46-89               c
            1396-1460           BY634907.1         355-419
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 55.38 cM"
     gene            1..1460
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /note="phosphopantothenoylcysteine synthetase"
                     /db_xref="GeneID:106564"
                     /db_xref="MGI:MGI:1915237"
     exon            1..575
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1003
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /EC_number="6.3.2.51"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphopantothenate--cysteine ligase; PPC synthetase"
                     /codon_start=1
                     /product="phosphopantothenate--cysteine ligase isoform 1"
                     /protein_id="NP_080770.2"
                     /db_xref="CCDS:CCDS18581.1"
                     /db_xref="GeneID:106564"
                     /db_xref="MGI:MGI:1915237"
                     /translation="MAEMDLVAELPRPAGAARWAEVMARFAARLGEQGRRVVLITSGG
                     TKVPLEARAVRFLDNFSSGRRGAASAEVFLAAGYGVLFLYRARSAFPYAHRFPPQAWL
                     SALRPSGPAQSGKLSLEAEENALPGFAAALQSYQEAAAAGTFLAVEFTTLADYLHLLQ
                     AAALALSPLGSSAMFYLAAAVSDFYIPVSEMPEHKIHSSGGPLQITMKMVPKMLSPLV
                     KDWAPKAFVVSFKLETDPDIIISRARNALEVYQHQVVVANILESIKSFVIIVTKDSET
                     ELLLSEEEVAKGLVIEEKIVDDLRSRHTAFICDKN"
     exon            576..679
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            680..1446
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1409..1414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1446
                     /gene="Ppcs"
                     /gene_synonym="6330579B17Rik"
                     /note="major polyA site"
ORIGIN      
        1 gggcgtggcg ccagcggccc ggcgtgcccg ggcgctgtgg ttctaccgtg gggtgactac
       61 agtgcagatg gcggaaatgg acctggtggc tgagttgccg cgtcctgcgg gtgctgcgcg
      121 ctgggccgag gttatggctc gctttgcggc caggctgggc gagcagggcc ggcgggtggt
      181 gctgatcaca tctggaggca ccaaggtccc cctggaagcg cgagccgtgc gctttctgga
      241 caacttcagt agcgggcgac gcggagccgc gtcggcggag gtcttcctgg ctgccggcta
      301 tggagtcctg ttcttgtacc gagcgcgctc ggccttcccc tatgcccacc gcttcccgcc
      361 ccaggcctgg ctgtcagccc ttcggccttc tggcccagcc cagtcgggca agctgagtct
      421 ggaggccgaa gagaatgcgc tcccgggctt tgctgcagca ttgcagagct accaagaggc
      481 agccgctgcc ggcacctttc tggctgtaga gtttaccact ttggcggatt atctgcatct
      541 gctgcaggct gctgccctgg ctctcagtcc attaggctct tctgcgatgt tttacctggc
      601 cgcggcagtg tcagatttct atattcctgt ctccgaaatg cctgaacaca agatccactc
      661 atctggtggc ccactgcaga taacaatgaa gatggtgcca aagatgcttt cccctctggt
      721 caaagactgg gctcctaaag cgtttgtagt ttcctttaag ctggagacag acccggacat
      781 cataatcagt cgggctcgga atgctttgga agtttaccag catcaggtgg tggtggccaa
      841 catcctggag tcaataaagt cctttgtgat tattgtaacc aaagactcgg agacagagtt
      901 actgctgtcc gaggaggagg tggcaaaagg cttggtgata gaagagaaga tagtagatga
      961 ccttcggtct cgacacacag cttttatatg tgacaaaaac tgaaggaaat gctcctctag
     1021 gatcaaacac cagagctctt accactgata ggaactaaga ttgatcctct gttaaggatc
     1081 aacacagtga agagatagta aacgcggtgt gcaggcaagt gcctgctggg acttcagttc
     1141 tgagagtgaa acactggacg ctctcctgga tctggaaagt gaacaagagt tcacaaagag
     1201 gactgtggga ttgcgtggcc tgcagagcag atgagccagg agccgctgcg tgttggccta
     1261 ctgtaaggtc atggtgcgtg aatggtcatg ctctgaagaa gtgtttatca agtgcctacc
     1321 atgtgccagg gcctttgtga gctgtgaggg tctgaaagat gaatgagcac gtctcatttg
     1381 ggaactggat gtacaaaaat ttatgtgcaa taaagtatgt gcccacaaag ggacacttgg
     1441 aaagggaaaa aaaaaaaaaa
//
