LOCUS       NM_172146               4008 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus phosphoribosyl pyrophosphate amidotransferase (Ppat),
            mRNA.
ACCESSION   NM_172146 XM_001002879 XM_001002886 XM_896000 XM_924520 XM_973937
            XM_973973
VERSION     NM_172146.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Testini,C., Smith,R.O., Jin,Y., Martinsson,P., Sun,Y., Hedlund,M.,
            Sainz-Jaspeado,M., Shibuya,M., Hellstrom,M. and Claesson-Welsh,L.
  TITLE     Myc-dependent endothelial proliferation is controlled by
            phosphotyrosine 1212 in VEGF receptor-2
  JOURNAL   EMBO Rep 20 (11), e47845 (2019)
   PUBMED   31545012
  REMARK    Erratum:[EMBO Rep. 2020 May 6;21(5):e50409. doi:
            10.15252/embr.202050409.. PMID: 32374524]
REFERENCE   3  (bases 1 to 4008)
  AUTHORS   Kurogi,S., Sekimoto,T., Funamoto,T., Ota,T., Nakamura,S., Nagai,T.,
            Nakahara,M., Yoshinobu,K., Araki,K., Araki,M. and Chosa,E.
  TITLE     Development of an efficient screening system to identify novel bone
            metabolism-related genes using the exchangeable gene trap
            mutagenesis mouse models
  JOURNAL   Sci Rep 7, 40692 (2017)
   PUBMED   28106071
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4008)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   5  (bases 1 to 4008)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 4008)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   7  (bases 1 to 4008)
  AUTHORS   Natsumeda,Y., Prajda,N., Donohue,J.P., Glover,J.L. and Weber,G.
  TITLE     Enzymic capacities of purine de Novo and salvage pathways for
            nucleotide synthesis in normal and neoplastic tissues
  JOURNAL   Cancer Res 44 (6), 2475-2479 (1984)
   PUBMED   6327016
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB647933.1, AK164506.1, AK147750.1, AK148034.1 and AK159859.1.
            
            On or before Jul 8, 2009 this sequence version replaced
            XM_001002886.2, XM_001002879.2, XM_924520.3, XM_973973.2,
            XM_973937.2, XM_896000.3, NM_172146.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148034.1, AK147750.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-387               BB647933.1         1-387
            388-1926            AK164506.1         2-1540
            1927-3182           AK147750.1         1577-2832
            3183-3926           AK148034.1         2787-3530
            3927-4008           AK159859.1         4078-4159
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 41.45 cM"
     gene            1..4008
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="phosphoribosyl pyrophosphate amidotransferase"
                     /db_xref="GeneID:231327"
                     /db_xref="MGI:MGI:2387203"
     exon            1..622
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             495..2048
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /EC_number="2.4.2.14"
                     /note="ATase; glutamine phosphoribosylpyrophosphate
                     amidotransferase"
                     /codon_start=1
                     /product="amidophosphoribosyltransferase"
                     /protein_id="NP_742158.1"
                     /db_xref="CCDS:CCDS51527.1"
                     /db_xref="GeneID:231327"
                     /db_xref="MGI:MGI:2387203"
                     /translation="MELEELGIREECGVFGCIASGDWPTQLDVPHVITLGLVGLQHRG
                     QESAGIVTSDGSAVPKFRVHKGMGLVNHVFTEDNLKKLYDSNLGIGHTRYATTGKCEL
                     ENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRQGIGLSTSSDSEMITQLLAYTPP
                     QEKDDAPDWVARIKNLMKEAPAAYSLVIMHRDFIYAVRDPYGNRPLCIGRLMPVSDVN
                     DKEKKSSETEGWVVSSESCSFLSIGARYCHEVKPGEIVEISRHGIRTLDIIPRSNGDP
                     VAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVEADLVSTVPESATPAALG
                     YATKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLIDDSI
                     VRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDCLAE
                     YLGANSVVYLSVEGLVSSVQQEIKFKKQKVKKHDIAIQENGNGLEYFEKTGHCTACLT
                     GQYPVELEW"
     exon            623..689
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            690..896
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1009
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1155
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1228
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1380
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1508
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1730
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1851
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..4008
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2189..2194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="hexamer: ATTAAA"
     polyA_site      2213
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="major polyA site"
     regulatory      3689..3694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="hexamer: TATAAA"
     polyA_site      3710
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
     regulatory      3906..3911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="hexamer: TATAAA"
     polyA_site      3929
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
     regulatory      3965..3970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
                     /note="hexamer: AATATA"
     polyA_site      3983
                     /gene="Ppat"
                     /gene_synonym="5730454C12Rik; GPAT"
ORIGIN      
        1 cccgcaggca gccaaaaagt ggcgcgcatg ctgcaaggcc cccgctggcc ggagaggttt
       61 ctgggttgga gcggaccaag tcctgacggc cgggggacgg cgtgagacca gggggcacga
      121 acggctcagg ttttctgagg gacccacgcg atcagctgcg ccactctcat cacctactcc
      181 ttgggcgcgg gaaaataacg gagcctccca cgtgcagaga aagtagcgat cctgacctcg
      241 gcctcctccc cacctctttg actccgtagc cccgcccctc tctttgccct agctgattgg
      301 cggagagcgg gaggcagaac tggtcacgcc ccttctatga agcgcgccgc cgcagtagcg
      361 agaacctagc ccgcccggga aggaggcagt cagtagcgcg agcctcttga ggcgttcgat
      421 agcggagcgg acagctggtt ggtgcttgga ccacgccgcg gcagcaggtc cctccacgtg
      481 ctttccgagg cgccatggag ctggaggagt tggggatccg cgaggaatgt ggtgtgtttg
      541 ggtgcatcgc ttctggagac tggcccacgc agctagatgt gccgcatgtg atcactctgg
      601 gactcgtggg gctacagcac cgaggtcagg agagcgctgg tattgtgacc agtgatggga
      661 gtgcagtgcc taaattcagg gtgcataagg gaatgggtct tgtaaaccat gtttttaccg
      721 aagacaattt gaagaaattg tatgactcaa accttggaat cggacatacg aggtacgcca
      781 ccacgggaaa gtgtgagctg gaaaactgcc agccattcgt agttgagacg cttcacggaa
      841 aaatagctgt ggcccataac ggggaactgg tgaacgccgc tcggttgcgg aagaagcttc
      901 tgcggcaggg tattgggctt tccacgtcct ctgatagtga aatgattacc cagttactcg
      961 catacacccc tcctcaagaa aaagatgatg ctccagactg ggtagcaaga attaagaact
     1021 taatgaaaga agcgcccgca gcatactccc tggttataat gcacagggac ttcatttacg
     1081 cggtgcgaga tccatatgga aatcgtcctc tctgcatcgg gcgtcttatg ccagtctctg
     1141 atgtaaatga caaagagaaa aaatcctcag aaactgaagg atgggtggtg tcctcagagt
     1201 cttgcagctt tttatccatc ggtgccagat attgccatga agtcaaaccc ggagaaattg
     1261 tagaaatatc cagacatggt atcagaactc ttgatataat tccaaggtct aacggagacc
     1321 cagtggcttt ttgtattttt gaatatgttt attttgcaag accagatagt atgtttgaag
     1381 accaaatggt ttatacagta aggtaccggt gtggtcagca gctggcaatt gaagcacccg
     1441 tggaggcgga cttggttagc acagttccag agtctgctac gcctgcagcg ctgggatatg
     1501 caacaaagtg tgggctgcct tatgtggaag tgctgtgtaa gaaccggtat gtaggaagaa
     1561 ccttcattca accaaacatg aggttaagac aactcggtgt tgcaaagaag tttggcgtgt
     1621 tgtctgacaa ctttaaaggc aaaagaatcg ttctcataga tgattcgatt gtgaggggca
     1681 atactatctc acccatcata aaactcctca aagaatccgg tgcaaaagag gtgcacattc
     1741 gagtcgcttc accaccaata aaatatccgt gcttcatggg aataaacatt cctacaaaag
     1801 aagagctcat agccaataag ccagaatttg attgtcttgc agagtacctc ggagcaaaca
     1861 gcgttgtgta tctgtcagtg gaaggactgg tttcatctgt gcaacaagaa ataaaattta
     1921 agaagcagaa agtgaaaaaa catgatattg caattcaaga aaatggaaat ggtttggaat
     1981 attttgaaaa gacgggacac tgtacagctt gcctcactgg gcagtaccct gtggagctgg
     2041 aatggtagct gcctggacag atgtgttgct tccagactgt agggtcatca agaagtggtc
     2101 tacatactgt tgttactcag gtggtaccaa gtggatacca catgcttatg tatgtatact
     2161 ttaagctttg agattttctg aggttattat taaattgcta tattgaacct gaagattaca
     2221 tggaatgcag catctgagga ttcattttgt acaaggactg acttttcact cgagagaagg
     2281 cagggggtta gtggcagttc ccagaaccat gcttgagggg tttaaaagag gctagagtat
     2341 agggctgtta tgtacagggg ctttataaag tttcatgaag aatttgagtt cattgtgccc
     2401 cataggtatc tagtctgtac agtcaagcct gtaattatgg ccttgacctt aagattcttc
     2461 caccccatac acgtgtggct ctgaggacca gtgtacaatt cttaaaaaat gaaaacctac
     2521 gtcggaagct gaggcagaat cctgagttca aggcgaacct gggctttcct gtctcaagat
     2581 aaaaagcctc tcctgtgccg ggacccccca tccatctgtc gttcatacac ttagtacata
     2641 gtaggaatgt gcgtgcctgt cataggtcgt ttagagctgc cgtctcaccc ctgtgcatgg
     2701 ccattagcat ctcagaagct agtgataatt accttttgct aaattacttt tcggtttcat
     2761 tgtgatggag ggaaatggac agctttgcag tcctttccca tctctcttgg ggatgtacca
     2821 gaatgactaa aaatatatct ggagcctttt tgttttccta cacttgagga gaaaatactt
     2881 agtctagcaa ataacttagt acattggaaa gagaaagttt taatttcaaa taagacttta
     2941 tcctaggttg attaaacctg gctttttgaa ttgagtgctt gatcattcac agaaatgtat
     3001 acttgtatac atattataca tatgatttaa acagagcaaa tagatgcata atcgggctga
     3061 ttttcttaaa ggccagtgtt ttgggaatta gattgcctgt acccaagtgt tagctcgcaa
     3121 tcatgtttgt tctgctttat gttgttaggc aggcctggtg acattttatt tttagttttt
     3181 ttatgatgca gatttagtgg attgagatga tttactaact cctgtttgtg taagattttt
     3241 tcacatggaa aaataactat ttctatattg ttgccaaaat ttgctatata ctttccaaac
     3301 ccacttagaa atataatatt tattaataac tgaaattatt agtctacatt tggtctatag
     3361 tatcatgtgt tacataatgg caggtcaaga taaaatgtta atactttaag gtcatgtgct
     3421 gcagtgcctg aggttatcgt atggcataga cacaggcgga ttggcatttc acagccccag
     3481 agccatttca gtgggtgcag cctcttatgt taaatgcatt tcccgtggca gtgaggaggg
     3541 gtaacgtaac gcctttaggt caacttttaa atggcctttt taaaatttga tgttttacac
     3601 agaaactcag ggaaggttct acaaccaaaa tctctaattt gacttttgtg tttttcatag
     3661 tttttatttt gttttattgg attactttta taaagcgaaa taataaccaa ggtcgtttgt
     3721 acaactttct taattatttc ttcattatat gcttttattt tacaagtttc tgaatatgga
     3781 ttaaattaac tttttttttt ttttaccagt ttcaagcctt gttttctctg ctttaaatgt
     3841 atataactag agtactatat gctcaaaaga tgaaaaatgt gtttgaacac aacatcccaa
     3901 actaatataa acctactttc ttttttggtt tttgcatctg attttttagg ggtggggggc
     3961 ggcaaatata tgtaataaat gtgtttatgt gtgcatttta aaaacagt
//
