LOCUS       NM_146141               1383 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus pyrophosphatase (inorganic) 2 (Ppa2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_146141 XM_915060
VERSION     NM_146141.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Distler,U., Schmeisser,M.J., Pelosi,A., Reim,D., Kuharev,J.,
            Weiczner,R., Baumgart,J., Boeckers,T.M., Nitsch,R., Vogt,J. and
            Tenzer,S.
  TITLE     In-depth protein profiling of the postsynaptic density from mouse
            hippocampus using data-independent acquisition proteomics
  JOURNAL   Proteomics 14 (21-22), 2607-2613 (2014)
   PUBMED   25211037
REFERENCE   3  (bases 1 to 1383)
  AUTHORS   Qi,Z., Yang,W., Liu,Y., Cui,T., Gao,H., Duan,C., Lu,L., Zhao,C.,
            Zhao,H. and Yang,H.
  TITLE     Loss of PINK1 function decreases PP2A activity and promotes
            autophagy in dopaminergic cells and a murine model
  JOURNAL   Neurochem Int 59 (5), 572-581 (2011)
   PUBMED   21672589
  REMARK    GeneRIF: downregulation of protein phosphatase 2 activity in
            PINK1-silenced cells promotes the protective effect of autophagy
            through phosphorylation of Bcl-2
REFERENCE   4  (bases 1 to 1383)
  AUTHORS   Xing,Y., Igarashi,H., Wang,X. and Sakaguchi,N.
  TITLE     Protein phosphatase subunit G5PR is needed for inhibition of B cell
            receptor-induced apoptosis
  JOURNAL   J Exp Med 202 (5), 707-719 (2005)
   PUBMED   16129705
REFERENCE   5  (bases 1 to 1383)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA444705.1, AK143475.1 and AK090384.1.
            
            On Jun 11, 2014 this sequence version replaced NM_146141.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011417.1, SRR1660811.332823.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AA444705.1         1-30
            31-1212             AK143475.1         2-1183
            1213-1383           AK090384.1         1199-1369
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 61.71 cM"
     gene            1..1383
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /note="pyrophosphatase (inorganic) 2"
                     /db_xref="GeneID:74776"
                     /db_xref="MGI:MGI:1922026"
     exon            1..172
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1023
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /EC_number="3.6.1.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; inorganic pyrophosphatase 2, mitochondrial;
                     PPase 2; pyrophosphate phospho-hydrolase 2"
                     /codon_start=1
                     /product="inorganic pyrophosphatase 2, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_666253.1"
                     /db_xref="CCDS:CCDS17850.1"
                     /db_xref="GeneID:74776"
                     /db_xref="MGI:MGI:1922026"
                     /translation="MRALLPLLSVGRGWRVGAAARPPRRVMSLYRTEELGHPRSQDYR
                     LFFKHVAGHYISPFHDIPLKADCKEEHDIPRKKARNDEYENLFNMVVEIPRWTNAKME
                     IATEEPLNPIKQDIKNGKLRYTPNIFPHKGYIWNYGALPQTWEDPHLRDKSTDCCGDN
                     DPIDVCEIGSKVLSRGDVVHVKILGTLALIDQSETDWKIIAINVNDPEAEKFHDIDDV
                     KKFKPGYLEATLNWFRLYKVPDGKPENKFAFNGEFKNKAFALDVINSAHERWKEMVMK
                     KCDKGAISCVNVHICDSPFHCTMEEARSLVESVPTPSMNKESNVEEEVWHFLRN"
     exon            173..237
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            238..282
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            283..336
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            337..456
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            457..543
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            544..670
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            671..798
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            799..884
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            885..954
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            955..994
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1383
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1194..1199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /note="hexamer: AATAAA"
     regulatory      1206..1211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /note="hexamer: AATAAA"
     polyA_site      1217
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
                     /note="major polyA site"
     polyA_site      1226
                     /gene="Ppa2"
                     /gene_synonym="1110013G13Rik; Sid6306"
ORIGIN      
        1 cgaccctgcg gttctcgcag accgtccgcc atgcgcgcgc tgctgccgct gctgagcgtc
       61 ggccgagggt ggcgcgtggg ggccgccgcc cgccctccgc gccgcgtcat gtccctgtac
      121 cgcacggagg agctgggcca tccccgctcg caggactacc gcctgttctt caagcatgta
      181 gctggtcatt acatctcccc tttccacgac attcctctga aggcggactg taaagaggaa
      241 catgacattc ccaggaaaaa ggcacgaaat gatgaatatg agaatttgtt taatatggtt
      301 gtggaaatac ctcggtggac aaatgccaag atggagattg ccacagagga gccactcaat
      361 cccattaagc aagatataaa gaacggaaag ctacgctaca caccgaacat cttccctcac
      421 aagggctata tatggaatta cggggccctc cctcagacct gggaagatcc tcacctaaga
      481 gataagagca ccgactgctg tggggataat gatcccattg acgtctgtga aataggctca
      541 aaggttcttt cccgtggaga cgtggtccat gtgaagatcc ttgggacttt ggcacttatt
      601 gatcagagtg aaacagattg gaaaataatt gctatcaatg tgaatgatcc tgaggctgaa
      661 aaatttcatg atattgatga tgttaagaag ttcaaaccag gttacctgga agccactctc
      721 aattggttta gactctataa ggtaccagat ggaaaaccag aaaacaagtt tgcttttaat
      781 ggagaattca aaaacaaggc tttcgccctc gacgttatta actctgcaca tgaacgttgg
      841 aaagagatgg ttatgaagaa gtgtgacaag ggagccatca gttgtgtaaa tgtgcacatc
      901 tgcgatagcc ctttccactg cactatggag gaagcaagat cattggttga atcggttcca
      961 acaccttcga tgaataagga aagcaatgta gaggaagaag tgtggcactt cctccgcaac
     1021 tgatggggac acagggcttc tgcaccgaga tgtcaacctc tggggacacg gggcttctgc
     1081 actgacgtca gcctccaggg actcccgggg ttccttctcc tttagtgctc ttgccgggca
     1141 cctggatggc catctgatga cttcctgtca gacatcactg ttaaaacttt taaaataaac
     1201 ttgtaaataa atactaacat tttgaacttc tcttttatat atttattaat aagagaaaga
     1261 aacggtttca cttaatttcg tatttgaatc taaaggcatt gtttagctaa tgattccatt
     1321 tacttttgtg tctaattggt taaatgtata atcaggaatt taacaataaa atttttacca
     1381 att
//
