LOCUS       NM_001195681            1274 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus POU class 2 homeobox associating factor 3 (Pou2af3),
            transcript variant 1, mRNA.
ACCESSION   NM_001195681 XM_196479 XM_924862
VERSION     NM_001195681.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160052.2.
            
            On Jul 26, 2022 this sequence version replaced NM_001195681.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861859.5138724.1,
                                           ERR3363658.4574701.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               AC160052.2         20334-20559
            227-321             AC160052.2         21209-21303
            322-376             AC160052.2         24964-25018
            377-606             AC160052.2         26342-26571
            607-1274            AC160052.2         27974-28641
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 28.02 cM"
     gene            1..1274
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /note="POU class 2 homeobox associating factor 3"
                     /db_xref="GeneID:100502940"
                     /db_xref="MGI:MGI:2685530"
     exon            1..226
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /inference="alignment:Splign:2.1.0"
     exon            227..321
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..981
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /note="COLCA2 homolog; colorectal cancer-associated
                     protein 2"
                     /codon_start=1
                     /product="POU Class 2 homeobox-associating factor 3"
                     /protein_id="NP_001182610.1"
                     /db_xref="CCDS:CCDS57676.1"
                     /db_xref="GeneID:100502940"
                     /db_xref="MGI:MGI:2685530"
                     /translation="MTVKELLQQRRAHQATSGANLSGSSGLHLPDTTMPSSAGLYFEP
                     EPTSSTPSYFQTREFSTCVSCEEIPSCLDQIFESYLQTDTLPEPLLNSAQIAPHYFPE
                     SCQVAPFCHNQSLIPGSPSDSSSLSGSFDCSYSPTQLPSYTPENYSSPPSLDSLHSSL
                     PEEGYFCQHWPSHPQYNHSSPATPSSVCYYASCEAEHLDALRTTEFFSYSGMDCADFA
                     PPVATTGDFYKRETSCDACYS"
     exon            322..376
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /inference="alignment:Splign:2.1.0"
     exon            377..606
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /inference="alignment:Splign:2.1.0"
     exon            607..1274
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1254..1259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /note="hexamer: AATAAA"
     polyA_site      1274
                     /gene="Pou2af3"
                     /gene_synonym="Colca2; Gm684; OCA-T2"
                     /note="major polyA site"
ORIGIN      
        1 gggatgccat tactccagag ctgcagctct tgctggagaa ctctccacag ctcagttctc
       61 caaaagcctg caccggaaag ggagggggtg ggaagagatg aagagagcga cagagtcgga
      121 gactagaggg aggaaggggg tgaggaagaa aagacatttt tgaaaaagag aaaggaccga
      181 caggagacta tttcccgatc ttggctcctt ttctggggtc agtgcaaaaa acctaaggtg
      241 tatcaaggcg tccgtgtgaa gatgaccgtg aaagagcttc tgcagcaaag aagggcacac
      301 caggccacct caggggcaaa cctgtcagga agcagcggtc tccaccttcc agacactacc
      361 atgccatctt ccgcaggact gtattttgag cctgaaccaa cgtcttccac acccagttat
      421 tttcaaactc gagaattttc cacctgtgtc tcttgtgaag aaattcctag ctgcctcgac
      481 cagatcttcg agtcctacct tcagacagac acactcccgg agcctttgct caactctgca
      541 cagattgctc cccactattt cccagagagc tgtcaagtgg cccctttctg ccacaaccag
      601 agcctgattc cagggtcgcc ttcggattct tccagtctct ctggctcctt tgattgcagt
      661 tactcaccaa ctcagctgcc ttcctacact ccagaaaatt acagctcgcc tccttctctg
      721 gattccctac actccagcct ccccgaagaa ggctacttct gccaacattg gccttctcac
      781 ccccagtaca accactcgtc cccagccacc ccctcctcgg tctgctacta tgcatcctgt
      841 gaggcagagc atctggatgc cctcagaacc acagagttct tctcctactc gggcatggac
      901 tgtgcggact ttgcccctcc tgtggccacc actggcgatt tctataagag ggagacaagc
      961 tgtgatgcct gctatagtta gtagaaacca tgatagaatg tgtcatgagt ctatctcttt
     1021 ctctactgca tcttgatgac acttcaacca ttcaaccctt tcacaaacta ccccacatat
     1081 ggtttacgtt tcaccatttt caatcacctg agagctagcg taggagtcaa cacaatgccc
     1141 tcaaacttgg tcttgtgaca gtcttatttt atacactcac tgtgaaaatt tttttgtatg
     1201 gctttttcca gtgccttcct tctcggtata acaaacaaac tcttttgtaa actaataaat
     1261 agctttgtat aata
//
