LOCUS       NM_133931               2938 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus protection of telomeres 1A (Pot1a), mRNA.
ACCESSION   NM_133931
VERSION     NM_133931.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Nakashima,K., Kunisaki,Y., Hosokawa,K., Gotoh,K., Yao,H., Yuta,R.,
            Semba,Y., Nogami,J., Kikushige,Y., Stumpf,P.S., MacArthur,B.D.,
            Kang,D., Akashi,K., Ohga,S. and Arai,F.
  TITLE     POT1a deficiency in mesenchymal niches perturbs B-lymphopoiesis
  JOURNAL   Commun Biol 6 (1), 996 (2023)
   PUBMED   37773433
  REMARK    GeneRIF: POT1a deficiency in mesenchymal niches perturbs
            B-lymphopoiesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Martinez,P., Sanchez-Vazquez,R., Ferrara-Romeo,I., Serrano,R.,
            Flores,J.M. and Blasco,M.A.
  TITLE     A mouse model for Li-Fraumeni-Like Syndrome with cardiac
            angiosarcomas associated to POT1 mutations
  JOURNAL   PLoS Genet 18 (6), e1010260 (2022)
   PUBMED   35727838
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2938)
  AUTHORS   Mizuno,T., Hirabayashi,K., Miyazawa,S., Kobayashi,Y., Shoji,K.,
            Kobayashi,M., Hanaoka,F., Imamoto,N. and Torigoe,H.
  TITLE     The intrinsically disordered N-terminal region of mouse DNA
            polymerase alpha mediates its interaction with POT1a/b at telomeres
  JOURNAL   Genes Cells 26 (6), 360-380 (2021)
   PUBMED   33711210
  REMARK    GeneRIF: The intrinsically disordered N-terminal region of mouse
            DNA polymerase alpha mediates its interaction with POT1a/b at
            telomeres.
REFERENCE   4  (bases 1 to 2938)
  AUTHORS   Markiewicz-Potoczny,M., Lobanova,A., Loeb,A.M., Kirak,O.,
            Olbrich,T., Ruiz,S. and Lazzerini Denchi,E.
  TITLE     TRF2-mediated telomere protection is dispensable in pluripotent
            stem cells
  JOURNAL   Nature 589 (7840), 110-115 (2021)
   PUBMED   33239785
REFERENCE   5  (bases 1 to 2938)
  AUTHORS   Rai,R., Gu,P., Broton,C., Kumar-Sinha,C., Chen,Y. and Chang,S.
  TITLE     The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1
            Independently of MRN Function
  JOURNAL   Cell Rep 29 (11), 3708-3725 (2019)
   PUBMED   31825846
REFERENCE   6  (bases 1 to 2938)
  AUTHORS   He,H., Multani,A.S., Cosme-Blanco,W., Tahara,H., Ma,J., Pathak,S.,
            Deng,Y. and Chang,S.
  TITLE     POT1b protects telomeres from end-to-end chromosomal fusions and
            aberrant homologous recombination
  JOURNAL   EMBO J 25 (21), 5180-5190 (2006)
   PUBMED   17053789
  REMARK    GeneRIF: shRNA mediated depletion of endogenous Pot1b in Pot1a
            deficient cells resulted in increased chromosomal aberrations
REFERENCE   7  (bases 1 to 2938)
  AUTHORS   Hockemeyer,D., Daniels,J.P., Takai,H. and de Lange,T.
  TITLE     Recent expansion of the telomeric complex in rodents: Two distinct
            POT1 proteins protect mouse telomeres
  JOURNAL   Cell 126 (1), 63-77 (2006)
   PUBMED   16839877
  REMARK    GeneRIF: We conclude that mouse telomeres require two distinct POT1
            proteins whereas human telomeres have one.
REFERENCE   8  (bases 1 to 2938)
  AUTHORS   Wu,L., Multani,A.S., He,H., Cosme-Blanco,W., Deng,Y., Deng,J.M.,
            Bachilo,O., Pathak,S., Tahara,H., Bailey,S.M., Deng,Y.,
            Behringer,R.R. and Chang,S.
  TITLE     Pot1 deficiency initiates DNA damage checkpoint activation and
            aberrant homologous recombination at telomeres
  JOURNAL   Cell 126 (1), 49-62 (2006)
   PUBMED   16839876
  REMARK    GeneRIF: Our results suggest that POT1a is crucial for the
            maintenance of both telomere integrity and overall genomic
            stability.
REFERENCE   9  (bases 1 to 2938)
  AUTHORS   Liu,D., O'Connor,M.S., Qin,J. and Songyang,Z.
  TITLE     Telosome, a mammalian telomere-associated complex formed by
            multiple telomeric proteins
  JOURNAL   J Biol Chem 279 (49), 51338-51342 (2004)
   PUBMED   15383534
REFERENCE   10 (bases 1 to 2938)
  AUTHORS   Baumann,P., Podell,E. and Cech,T.R.
  TITLE     Human Pot1 (protection of telomeres) protein: cytolocalization,
            gene structure, and alternative splicing
  JOURNAL   Mol Cell Biol 22 (22), 8079-8087 (2002)
   PUBMED   12391173
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158683.2 and AC153888.3.
            
            On May 29, 2018 this sequence version replaced NM_133931.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK040864.1, SRR7652917.360864.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC158683.2         95836-95879         c
            45-111              AC158683.2         92536-92602         c
            112-338             AC158683.2         86945-87171         c
            339-385             AC158683.2         84670-84716         c
            386-500             AC158683.2         81221-81335         c
            501-631             AC158683.2         65500-65630         c
            632-922             AC158683.2         62186-62476         c
            923-1078            AC158683.2         58180-58335         c
            1079-1251           AC158683.2         50913-51085         c
            1252-1337           AC158683.2         45479-45564         c
            1338-1400           AC158683.2         43886-43948         c
            1401-1557           AC158683.2         42824-42980         c
            1558-1763           AC158683.2         39810-40015         c
            1764-1899           AC158683.2         39021-39156         c
            1900-1988           AC153888.3         178467-178555       c
            1989-2080           AC153888.3         176708-176799       c
            2081-2186           AC153888.3         174308-174413       c
            2187-2938           AC153888.3         172359-173110       c
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.65 cM"
     gene            1..2938
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /note="protection of telomeres 1A"
                     /db_xref="GeneID:101185"
                     /db_xref="MGI:MGI:2141503"
     exon            1..44
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            45..111
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            112..338
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            339..385
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..2299
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /note="POT1-like telomere end-binding protein"
                     /codon_start=1
                     /product="protection of telomeres protein 1"
                     /protein_id="NP_598692.1"
                     /db_xref="CCDS:CCDS39442.1"
                     /db_xref="GeneID:101185"
                     /db_xref="MGI:MGI:2141503"
                     /translation="MSLVSTAPYTYTPLNLLKEGTIANVYGVVKFFKPPYVSKGTDYC
                     SVVTIVDQTNVKLTCMLFSGNYEALPIIYKVGDIVRFHRLKIQVYKNELQGINCSGFA
                     SLTFEGTVGMPVTARTSSKVFSFTPQDQKMVEALRVWASKHISASSTLVQLCDAQPMQ
                     YYDLTCQLLGKAQVDSTAFLLKVWDGTQTVLPSWRVSTQDLTFEGDLSHIERLQSLVV
                     DILVYDNHVQVARSIEVGCFLRLYSLHTKLQPGNSETSSSESLRLEFHLHGGTSYGRG
                     IRVLPDTSPCVDQLKKALEGANLPVTETSTGICQSENGDSSALSNSGSGAVSPYEEER
                     CQQVSATILTNHQHLEKTPLCAILTQKAPQQYRVRAKLRSYLPRRLSQSVKLLCPKCH
                     SVQEVPHGDSLDKILQDAATEAPDIKLKATSLYYSKVWTTEDQGGRQVAVHFVKNNGI
                     LPASSECLILIEGGRLCEVSKLSSKFHSVMPVRSGPESLELLTLSAPFLIQGKVHHYG
                     CKQCSSLKPIQNLNSRFHKGPWTPSSVAEALGVVPLQYVFVMVFTLDDGTGVLEAYLK
                     DSEHFFKIPASEVLTDDDLQRSLETIMDMICPPGIKVDAYPWLECLLKSYNVTIGTER
                     RICYQIFDTTVAENVV"
     exon            386..500
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            501..631
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            632..922
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1078
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1251
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1337
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1400
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1557
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1763
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1899
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1988
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2080
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2186
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2938
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2917..2922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /note="hexamer: AATAAA"
     polyA_site      2938
                     /gene="Pot1a"
                     /gene_synonym="1500031H18Rik; Pot1"
                     /note="major polyA site"
ORIGIN      
        1 acaggcgcct aggtccagac tttagttgtc cgcgggtttt gctgggaaaa ctcagttgct
       61 ccttaatgct ttgtggaagt caagcagctg ttttgaagat tatactttaa gaggctggat
      121 tgtcctaaca aggagcatct gcatgctaca gaggctgaaa ccccatagct gttcagccaa
      181 tctgtttgat gcctcagcag tctctatctt cggttgtgga aagcctacag aatttctgca
      241 gaacctttgg aagactcaag aagctggcat ctgatgtcag taaagtatag cagcagcagc
      301 agcagtgggg ctgatgcact caccagcagg aatgaaagat ttatgataaa tctgtgagag
      361 gatttttcat caaacaatgt ctttggtttc aacagctccc tatacatata cacccctgaa
      421 tctactcaag gaaggtacaa ttgccaatgt ctatggtgtt gtgaaattct ttaagcctcc
      481 gtatgttagc aaaggaactg attattgctc agttgtaaca attgtggacc agacaaatgt
      541 gaagttaacc tgtatgctct ttagtggaaa ctatgaagcc ctccccatca tttataaagt
      601 tggagacatt gttcgcttcc acaggctgaa gatccaagtg tataaaaatg agcttcaggg
      661 aatcaactgc tctggcttcg cctctttgac atttgaaggg actgtaggga tgcctgtcac
      721 agcacgcact tccagcaaag tttttagctt cactcctcag gaccaaaaaa tggtagaagc
      781 tttgcgggtt tgggcatcca agcacatctc ggcgtcttca actttagtgc agctgtgcga
      841 tgcgcagccc atgcagtatt acgacctgac ctgtcagctc ctgggtaaag cacaagtgga
      901 ttccacagca tttcttctaa aggtatggga tggaacccag actgtgcttc cctcctggag
      961 agtctccaca caagatctaa cttttgaagg tgacttgagt cacatcgaac ggctacagag
     1021 tctggtggta gacattctag tctacgataa ccacgttcaa gtggcgagat ccatcgaggt
     1081 tggatgcttt ctaagactct atagcctcca tacaaaactt cagcctggaa attcagaaac
     1141 cagcagttct gaatcattaa ggctggagtt ccatctccac ggagggacca gttatggtcg
     1201 gggcattagg gttttgccag atacaagccc ttgtgtggat cagctgaaaa aagctttaga
     1261 aggtgcaaat ttgccagtca ctgagacctc aactggtatc tgtcaatcag aaaatgggga
     1321 cagctctgca ctatcaaact caggatcagg tgcagtatca ccttatgaag aagaacgatg
     1381 tcaacaagtc tctgctacaa tacttaccaa tcaccaacat ttagagaaaa caccactgtg
     1441 tgccatcttg acgcaaaaag ctcctcaaca atatcgagtc cgcgccaaac tgagatccta
     1501 tttgcccagg agactttccc agtctgttaa acttctctgt cctaaatgtc attcagtaca
     1561 agaagttcca catggggaca gtttggataa aattttgcaa gatgctgcaa ctgaagctcc
     1621 agacatcaag ctgaaagcga catcattgta ttattcaaaa gtctggacca ctgaagacca
     1681 gggcggacgg caggtcgctg ttcattttgt gaaaaataat ggcattctcc ctgcttcaag
     1741 tgaatgtcta attttgatag aaggaggaag gctctgcgaa gtctctaaac tgtccagcaa
     1801 gttccacagt gtgatgcctg tgcggtccgg ccctgagagc ctggagctcc ttaccctttc
     1861 agccccgttc ctcatacagg gaaaggtgca tcactatgga tgtaaacagt gctcaagctt
     1921 gaaacctata caaaatctaa actcccgttt tcataaagga ccgtggactc cttcctctgt
     1981 ggcagaagcc ctgggcgttg tgccgctgca gtatgtgttt gttatggttt tcactctgga
     2041 tgacgggaca ggggtgttgg aagcttacct caaggattct gaacactttt tcaagatccc
     2101 agcatctgaa gtcctcactg atgacgacct tcagagaagt ctagaaacga tcatggatat
     2161 gatatgtccc ccaggaataa aagttgatgc atacccatgg ctagagtgcc tcctcaagtc
     2221 ctacaatgtc accatcggaa cagagcgacg aatttgctac cagatttttg atactacagt
     2281 tgcagaaaat gttgtctagt actgccattg agtgcagtgt gtatgggatt ttacagtttt
     2341 agaaaagatc atgactcttt gagattccca gaagacattt tgctattgtg aaaaagtgtt
     2401 ttatcctttt tcactatagt ttagtaattt gtacagaaaa atattttatt gttatttatg
     2461 ctgtcttgtg aacatatcaa aaatcccttg ttgctcatca tgtcgtggga ggtacaaaat
     2521 gaggcagcac tggttgggga gaaccaggct ttcttggctc tcatttataa aacactggga
     2581 gctgatttta cttctgtgct gtttgatgtt tgggtttttg ttgttgttgt tgttgtttgt
     2641 tttgttttgt tttttatacc gaagaatctg cttagcaact tctgaacgga ttctccctct
     2701 gcatccacac atgagtatca ggttaactgc gtgccggttg aacagatgct tgtctcggtg
     2761 agcctggctg tactgatgta ctttcagaag acgtatggtt tatttgggtg ctctggtcgc
     2821 ccactttagc gtaagtgtag ctctgtgaac cgaagagtat gcctgacctt cagcatttct
     2881 ctacaacatt agaagttgct tcattgccca ccagcgaata aagtgatgag atttatga
//
