LOCUS       NM_008898               2527 bp    mRNA    linear   ROD 07-FEB-2024
DEFINITION  Mus musculus cytochrome p450 oxidoreductase (Por), mRNA.
ACCESSION   NM_008898
VERSION     NM_008898.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Yan,B., Ai,Y., Sun,Q., Ma,Y., Cao,Y., Wang,J., Zhang,Z. and Wang,X.
  TITLE     Membrane Damage during Ferroptosis Is Caused by Oxidation of
            Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1
  JOURNAL   Mol Cell 81 (2), 355-369 (2021)
   PUBMED   33321093
  REMARK    GeneRIF: Membrane Damage during Ferroptosis Is Caused by Oxidation
            of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Reed,L., Indra,R., Mrizova,I., Moserova,M., Schmeiser,H.H.,
            Wolf,C.R., Henderson,C.J., Stiborova,M., Phillips,D.H. and
            Arlt,V.M.
  TITLE     Application of hepatic cytochrome b5/P450 reductase null (HBRN)
            mice to study the role of cytochrome b5 in the cytochrome
            P450-mediated bioactivation of the anticancer drug ellipticine
  JOURNAL   Toxicol Appl Pharmacol 366, 64-74 (2019)
   PUBMED   30685480
  REMARK    GeneRIF: Hepatic cytochrome b5/P450 reductase contribute to the
            bioactivation of ellipticine in vitro, but not in vivo.
REFERENCE   3  (bases 1 to 2527)
  AUTHORS   Barzi,M., Pankowicz,F.P., Zorman,B., Liu,X., Legras,X., Yang,D.,
            Borowiak,M., Bissig-Choisat,B., Sumazin,P., Li,F. and Bissig,K.D.
  TITLE     A novel humanized mouse lacking murine P450 oxidoreductase for
            studying human drug metabolism
  JOURNAL   Nat Commun 8 (1), 39 (2017)
   PUBMED   28659616
  REMARK    Erratum:[Nat Commun. 2017 Oct 17;8(1):984. PMID: 29042563]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2527)
  AUTHORS   Rezende,F., Prior,K.K., Lowe,O., Wittig,I., Strecker,V., Moll,F.,
            Helfinger,V., Schnutgen,F., Kurrle,N., Wempe,F., Walter,M.,
            Zukunft,S., Luck,B., Fleming,I., Weissmann,N., Brandes,R.P. and
            Schroder,K.
  TITLE     Cytochrome P450 enzymes but not NADPH oxidases are the source of
            the NADPH-dependent lucigenin chemiluminescence in membrane assays
  JOURNAL   Free Radic Biol Med 102, 57-66 (2017)
   PUBMED   27863990
  REMARK    GeneRIF: Knockout of the cytochrome P450 reductase by CRISPR/Cas9
            technology (POR(-/-)) in HEK293 cells overexpressing Nox4 or Nox5
            did not interfere with ROS production in intact cells. However,
            POR(-/-) abolished the signal in NADPH-stimulated assays using
            membrane fractions from the very same cells. Moreover, membranes of
            rat smooth muscle cells treated with angiotensin II showed an
            increased NADPH-dependent signal with lucigen
REFERENCE   5  (bases 1 to 2527)
  AUTHORS   Wang,H., Fan,Z., Zhao,M., Li,J., Lu,M., Liu,W., Ying,H., Liu,M. and
            Yan,J.
  TITLE     Oscillating primary transcripts harbor miRNAs with circadian
            functions
  JOURNAL   Sci Rep 6, 21598 (2016)
   PUBMED   26898952
  REMARK    GeneRIF: we observed that miR-378 modulates the oscillation
            amplitudes of Cdkn1a in the control of cell cycle and Por in the
            regulation of oxidation reduction by forming partnership with
            different circadian transcription factors
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2527)
  AUTHORS   Keeney,D.S., Ikeda,Y., Waterman,M.R. and Parker,K.L.
  TITLE     Cholesterol side-chain cleavage cytochrome P450 gene expression in
            the primitive gut of the mouse embryo does not require
            steroidogenic factor 1
  JOURNAL   Mol Endocrinol 9 (8), 1091-1098 (1995)
   PUBMED   7476982
REFERENCE   7  (bases 1 to 2527)
  AUTHORS   Ohgiya,S., Goda,T., Ishizaki,K., Kamataki,T. and Shinriki,N.
  TITLE     Molecular cloning and sequence analysis of guinea-pig
            NADPH-cytochrome P-450 oxidoreductase [corrected]
  JOURNAL   Biochim Biophys Acta 1171 (1), 103-105 (1992)
   PUBMED   1420354
  REMARK    Erratum:[Biochim Biophys Acta. 1993 Sep 23;1174(3):313. PMID:
            8373812]
REFERENCE   8  (bases 1 to 2527)
  AUTHORS   Shephard,E.A., Phillips,I.R., Santisteban,I., West,L.F.,
            Palmer,C.N., Ashworth,A. and Povey,S.
  TITLE     Isolation of a human cytochrome P-450 reductase cDNA clone and
            localization of the corresponding gene to chromosome 7q11.2
  JOURNAL   Ann Hum Genet 53 (4), 291-301 (1989)
   PUBMED   2516426
REFERENCE   9  (bases 1 to 2527)
  AUTHORS   Simmons,D.L., Lalley,P.A. and Kasper,C.B.
  TITLE     Chromosomal assignments of genes coding for components of the
            mixed-function oxidase system in mice. Genetic localization of the
            cytochrome P-450PCN and P-450PB gene families and the
            nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes
  JOURNAL   J Biol Chem 260 (1), 515-521 (1985)
   PUBMED   3917435
REFERENCE   10 (bases 1 to 2527)
  AUTHORS   Gonzalez,F.J. and Kasper,C.B.
  TITLE     Cloning of DNA complementary to rat liver NADPH-cytochrome c
            (P-450) oxidoreductase and cytochrome P-450b mRNAs. Evidence that
            phenobarbital augments transcription of specific genes
  JOURNAL   J Biol Chem 257 (10), 5962-5968 (1982)
   PUBMED   6802825
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC083948.3.
            
            [WARNING] On Mar 15, 2024 this sequence was replaced by
            NM_008898.3.
            
            On May 5, 2015 this sequence version replaced NM_008898.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031463.1, D17571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AC083948.3         74183-74290
            109-300             AC083948.3         100910-101101
            301-349             AC083948.3         113700-113748
            350-478             AC083948.3         114437-114565
            479-628             AC083948.3         115267-115416
            629-753             AC083948.3         115882-116006
            754-843             AC083948.3         116131-116220
            844-942             AC083948.3         116601-116699
            943-1059            AC083948.3         117521-117637
            1060-1178           AC083948.3         117724-117842
            1179-1360           AC083948.3         118540-118721
            1361-1510           AC083948.3         118811-118960
            1511-1781           AC083948.3         119065-119335
            1782-1927           AC083948.3         119421-119566
            1928-2013           AC083948.3         119659-119744
            2014-2527           AC083948.3         119851-120364
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 75.34 cM"
     gene            1..2527
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /note="cytochrome p450 oxidoreductase"
                     /db_xref="GeneID:18984"
                     /db_xref="MGI:MGI:97744"
     exon            1..108
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            109..300
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..2158
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /EC_number="1.6.2.4"
                     /note="NADH cytochrome P450 oxydoreductase; P450
                     (cytochrome) oxidoreductase"
                     /codon_start=1
                     /product="NADPH--cytochrome P450 reductase"
                     /protein_id="NP_032924.1"
                     /db_xref="CCDS:CCDS39318.1"
                     /db_xref="GeneID:18984"
                     /db_xref="MGI:MGI:97744"
                     /translation="MGDSHEDTSATVPEAVAEEVSLFSTTDIVLFSLIVGVLTYWFIF
                     KKKKEEIPEFSKIQTTAPPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDA
                     HRYGMRGMSADPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETD
                     VDLTGVKFAVFGLGNKTYEHFNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFIT
                     WREQFWPAVCEFFGVEATGEESSIRQYELVVHEDMDTAKVYTGEMGRLKSYENQKPPF
                     DAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSTLVNQI
                     GEILGADLDVIMSLNNLDEESNKKHPFPCPTTYRTALTYYLDITNPPRTNVLYELAQY
                     ASEPSEQEHLHKMASSSGEGKELYLSWVVEARRHILAILQDYPSLRPPIDHLCELLPR
                     LQARYYSIASSSKVHPNSVHICAVAVEYEAKSGRVNKGVATSWLRTKEPAGENGRRAL
                     VPMFVRKSQFRLPFKPTTPVIMVGPGTGVAPFMGFIQERAWLREQGKEVGETLLYYGC
                     RRSDEDYLYREELARFHKDGALTQLNVAFSREQAHKVYVQHLLKRDKEHLWKLIHEGG
                     AHIYVCGDARNMAKDVQNTFYDIVAEFGPMEHTQAVDYVKKLMTKGRYSLDVWS"
     exon            301..349
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            350..478
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            479..628
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            629..753
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            754..843
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            844..942
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1059
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1178
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1360
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1510
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1781
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1927
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2013
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2527
                     /gene="Por"
                     /gene_synonym="4933424M13Rik; CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggccgtggt agcgcctcag tggtgcgggc ttgcgtccgg ccccagtgcc tcagagacct
       61 acaggaccgc gcgcggtgtg tgatctggtc ggtaccgagg agcgcaggtt gtgtcaccaa
      121 catgggggac tctcacgaag acaccagtgc cacagtgcct gaggcagtgg ctgaagaagt
      181 gtctctattc agcacaacgg acattgttct gttttctctc atcgtggggg tcctgaccta
      241 ctggttcatc tttaaaaaga agaaagaaga gataccggag ttcagcaaga tccagacaac
      301 ggccccacct gtcaaagaga gcagcttcgt ggaaaagatg aagaaaacgg gaaggaacat
      361 tattgtattc tatggctccc agacgggaac cgcggaggag tttgccaacc ggctgtccaa
      421 ggatgcccac cgctatggga tgcggggcat gtctgcagac cctgaagagt atgacttggc
      481 cgacctgagc agcctgcctg agatcgacaa gtccctggta gtcttctgca tggccacata
      541 cggagaaggc gaccccaccg acaacgcgca ggacttctat gattggctgc aggagactga
      601 cgtggacctc acgggtgtca agtttgctgt gtttggtctc gggaacaaga cctatgagca
      661 cttcaacgcc atgggcaagt atgtggacca gcggctggag cagcttggcg cccagcgaat
      721 ctttgagttg ggccttggtg atgacgacgg gaacttggaa gaggatttca tcacatggag
      781 ggagcagttc tggccagctg tgtgcgagtt cttcggggtg gaagccactg gggaggagtc
      841 gagcatccgc cagtacgagc tcgtggtcca cgaagacatg gacacagcca aggtgtacac
      901 gggtgagatg ggccgtctga agagctacga gaaccagaaa ccccccttcg atgccaagaa
      961 tccattcctg gctgctgtca ccacgaaccg gaagctgaac caaggcactg agaggcatct
     1021 aatgcacctg gaattggaca tctcagactc caagatcagg tatgaatctg gagatcacgt
     1081 ggctgtgtac ccagccaacg actccaccct ggtcaaccag attggggaga tcctgggggc
     1141 tgacctggat gtcatcatgt ctctaaacaa tctcgatgag gagtcgaata agaagcatcc
     1201 gttcccctgc cccaccacct accgcacggc cctcacctac tacctggaca tcactaaccc
     1261 gccacgaacc aacgtgctct acgagctggc ccagtacgcc tcagagccct cggagcagga
     1321 acacctgcac aagatggcgt cctcctccgg cgagggcaag gagctgtacc tgagctgggt
     1381 ggtggaggcc cggaggcaca tcctagccat tctccaagac tacccgtccc tgcggccacc
     1441 catcgaccac ctgtgcgagc tcctcccgag gctgcaggcc cgctactatt ccattgcctc
     1501 gtcgtctaag gtccacccca actccgtgca catctgcgcc gtggctgtgg agtatgaagc
     1561 gaagtctgga cgagtgaaca agggggtggc caccagctgg cttcggacca aggaaccagc
     1621 aggagagaat ggccgccggg ccctggtccc catgttcgtc cgcaagtccc agttccgctt
     1681 gcctttcaag cccaccacac ctgttatcat ggtgggcccc ggcactgggg ttgccccttt
     1741 catgggcttc atccaggagc gggcttggct tcgagagcaa ggcaaggagg tcggagagac
     1801 gctgctctac tacggctgcc ggcgctcgga tgaggactat ctgtaccgcg aggagctggc
     1861 gcgcttccac aaggacggcg ccctcacgca gcttaatgtg gccttttccc gtgagcaggc
     1921 ccacaaggtc tatgttcagc acctgctcaa gagggacaaa gagcacctgt ggaagctgat
     1981 ccacgaaggt ggtgcccaca tctatgtctg cggggatgct cgaaatatgg ccaaagatgt
     2041 gcagaacaca ttctatgaca tcgtggccga gtttgggccc atggagcaca cccaggctgt
     2101 ggactatgtt aagaagctca tgaccaaggg ccgctactcg ctggatgtat ggagctagga
     2161 gctgccgccc cccacccctc gctccctgta atcacgtcct taacttcctt ctgccgacct
     2221 ccacctctgg tggttcctgc cctgcctgga cacagggagg cccagggact gactcctggc
     2281 ctgagtgatg ccctcctggg cccttaggca gagcctggtc cattgtacca ggcagcctag
     2341 cccagcccag ggcacatggc aagagggact ggacccacct ttgggtgatg ggtgccttag
     2401 gtccccagca gctgtacaga aggggctctt ctctccacag agctggggtg cagccccaac
     2461 atgtgatttt gaatgagtgt aaataatttt aaataacctg gcccttggaa taaagttgtt
     2521 ttctgta
//
