LOCUS       NM_025298               4203 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus polymerase (RNA) III (DNA directed) polypeptide E
            (Polr3e), transcript variant 1, mRNA.
ACCESSION   NM_025298
VERSION     NM_025298.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Yeganeh,M., Praz,V., Cousin,P. and Hernandez,N.
  TITLE     Transcriptional interference by RNA polymerase III affects
            expression of the Polr3e gene
  JOURNAL   Genes Dev 31 (4), 413-421 (2017)
   PUBMED   28289142
  REMARK    GeneRIF: a mammalian interspersed repeat (MIR) nested in antisense
            orientation within the first intron of the Polr3e gene, affects
            Polr3e expression through transcriptional interference
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Riccomagno,M.M., Sun,L.O., Brady,C.M., Alexandropoulos,K., Seo,S.,
            Kurokawa,M. and Kolodkin,A.L.
  TITLE     Cas adaptor proteins organize the retinal ganglion cell layer
            downstream of integrin signaling
  JOURNAL   Neuron 81 (4), 779-786 (2014)
   PUBMED   24559672
REFERENCE   3  (bases 1 to 4203)
  AUTHORS   Danzl,N.M., Donlin,L.T. and Alexandropoulos,K.
  TITLE     Regulation of medullary thymic epithelial cell differentiation and
            function by the signaling protein Sin
  JOURNAL   J Exp Med 207 (5), 999-1013 (2010)
   PUBMED   20404100
  REMARK    GeneRIF: Results identify Sin as a novel regulator of medullary
            thymic epithelial cell development and t cell tolerance.
REFERENCE   4  (bases 1 to 4203)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   5  (bases 1 to 4203)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 4203)
  AUTHORS   Dong,Z. and Bell,L.R.
  TITLE     SIN, a novel Drosophila protein that associates with the RNA
            binding protein sex-lethal
  JOURNAL   Gene 237 (2), 421-428 (1999)
   PUBMED   10521666
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122248.5.
            
            On Mar 28, 2020 this sequence version replaced NM_025298.3.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037637.2, AK146279.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-133               AC122248.5         89149-89281
            134-203             AC122248.5         94270-94339
            204-254             AC122248.5         95593-95643
            255-332             AC122248.5         98315-98392
            333-448             AC122248.5         99259-99374
            449-531             AC122248.5         99799-99881
            532-639             AC122248.5         102250-102357
            640-689             AC122248.5         102632-102681
            690-809             AC122248.5         103505-103624
            810-895             AC122248.5         104678-104763
            896-940             AC122248.5         105057-105101
            941-1032            AC122248.5         105204-105295
            1033-1153           AC122248.5         106144-106264
            1154-1235           AC122248.5         108974-109055
            1236-1295           AC122248.5         110367-110426
            1296-1453           AC122248.5         110510-110667
            1454-1515           AC122248.5         111120-111181
            1516-2039           AC122248.5         111673-112196
            2040-2117           AC122248.5         113861-113938
            2118-2243           AC122248.5         115918-116043
            2244-4203           AC122248.5         116847-118806
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 65.01 cM"
     gene            1..4203
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="polymerase (RNA) III (DNA directed) polypeptide E"
                     /db_xref="GeneID:26939"
                     /db_xref="MGI:MGI:1349452"
     exon            1..133
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            134..203
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..2300
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     polymerase III polypeptide E; DNA-directed RNA polymerase
                     III subunit RPC5; RNA polymerase III subunit 5; RNA
                     polymerase III subunit C5; sex-lethal interactor homolog;
                     sxl interactor"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC5
                     isoform 1"
                     /protein_id="NP_079574.2"
                     /db_xref="CCDS:CCDS21797.1"
                     /db_xref="GeneID:26939"
                     /db_xref="MGI:MGI:1349452"
                     /translation="MANEEDDPVIQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPH
                     LSAKIKPKQQKVELEMAIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTF
                     CSSQTTSNTARYAAALYRQGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGD
                     SSQDEAEEDVKQITVRFSRPESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGMRDSRS
                     EHERQYLLCQGSSGVENTELVKSPSEYLMMLMPPSPEEEKDKPVAPSNVLSMAQLRTL
                     PLADQIKVLMKNVKVMPFANLMSLLGPSVDSVAVLRGIQKVAMLVQGNWVVKSDILYP
                     KDSSSPHSGMPAEVLCRGRDFVMWKFTQSRWVVRKEVAAVTKLCAEDVKDFLEHMAVV
                     RINKGWEFLLPYDLEFIKKHPDVVQRQHMLWSGIQAKLEKVYNLVKETMPKKPDGQSA
                     PVGLVSGEQRVQTAKTKAQQNHAFLERELQRRKEQMRAATVLPSVQIKEEPLSEEEAD
                     GAELEAEEEEPMDTAPSTCLSTKLANGLPAGRAVGGDSLNGHPVPGCASNPVACELKA
                     FVEATFQRQFVLTLSELKRLFNLHLAGLPPGHILFSGVSDRMLQDTVLAAGCKQILVP
                     FPPQTAASPDEQKVFALWESGDMSDQHRQVLLEIFSKNYRVRRNLIQSRLTQECGEEL
                     SKQEVDKVLKDCCVSCGGMWYLKGTVQS"
     exon            204..254
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            255..332
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            333..448
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            449..531
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            532..639
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            640..689
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            690..809
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            810..895
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            896..940
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1032
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1153
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1235
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1295
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1453
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1515
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..2039
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2117
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2243
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..4203
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2570..2575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="hexamer: AATAAA"
     polyA_site      2610
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
     regulatory      2999..3004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="hexamer: AATAAA"
     polyA_site      3017
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="major polyA site"
     regulatory      4179..4184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
                     /note="hexamer: AATAAA"
     polyA_site      4203
                     /gene="Polr3e"
                     /gene_synonym="RPC5; Sin"
ORIGIN      
        1 ccccccacgt gtccggcgga gtttctccag caggaacatg gccgctgcct gatagaagac
       61 ccggccgccg ccgtctctgc agcccgcggg tccctgggcc gttccgccgc ccgcgcgcgg
      121 ccccggcgga gagatcgagt tgaggactgt gaggtgggct ccctagtatg gccaatgaag
      181 aagatgaccc agtcatacag gagatcgatg tgtacttggc taaaagtctg gcagagaagc
      241 tgtatctctt tcagtatcct gtgcgtcctg cttcaatgac ctatgatgac attccacatc
      301 tctcagccaa gatcaagccc aaacagcaga aggtagagct tgagatggcc atcgacaccc
      361 tgaatcccaa ctactgccgc agcaaagggg agcagatcgc actgaacgtg gacggtgcct
      421 gtgctgacga gactagcaca tattcctcaa agctgatgga caagcagacc ttctgctcct
      481 cccagaccac cagcaacaca gctcgttatg ccgctgcact ctacaggcaa ggtgagctcc
      541 acctgacacc tttacatggc atcctacagc ttcggcccag cttctcctac cttgataagg
      601 cagatgccaa gcaccgagag agggaggcag ccaatgaggc aggggattct tctcaggacg
      661 aggcagaaga agacgttaag cagatcacgg tacggttctc caggccagag tccgagcagg
      721 ctcgccagcg ccgcgtgcag tcctatgagt tcttgcagaa gaagcatgca gaagaaccct
      781 gggtccacct gcactactat ggcatgaggg acagccgctc agagcatgag cgccagtacc
      841 tgctgtgtca gggatccagc ggggtagaga acacggagct cgtcaagtca cccagtgaat
      901 acctcatgat gctcatgccg cccagcccag aggaggagaa ggacaagcct gtggctccca
      961 gcaatgtctt atctatggcc cagctgcgta cattgcctct ggctgatcag attaaggtcc
     1021 tgatgaagaa cgtgaaagtc atgcccttcg ctaatctcat gagcctcctg ggcccctctg
     1081 tggactctgt ggctgttcta cgtggcatcc agaaggtggc gatgttagtc caaggaaact
     1141 gggtggtaaa gagtgacatc ctgtatccca aggattcttc cagccctcac agtggcatgc
     1201 ctgctgaggt gctctgcagg ggccgagact ttgttatgtg gaagttcaca cagagccgat
     1261 gggtggtaag aaaggaggtg gcagcagtga ccaaactctg cgctgaggat gtgaaggact
     1321 ttctggagca catggctgta gtgagaatca acaaaggctg ggagttctta ctgccttacg
     1381 acctggagtt tatcaagaag catccagatg tggtccagcg gcagcacatg ctgtggtcgg
     1441 gcatccaggc caaattagaa aaagtctata atctcgtaaa agaaaccatg ccaaagaagc
     1501 cggatggaca atcagcgcct gttggtctgg tctctgggga acagcgggtc caaacagcca
     1561 aaaccaaggc ccagcagaac catgccttct tggagcgtga actgcagcga cggaaggagc
     1621 agatgcgggc agccacagtc ctgcccagcg tgcagatcaa ggaggaaccc ctgagtgagg
     1681 aggaagccga tggggctgag ctggaggctg aggaagagga gcccatggac actgcaccca
     1741 gcacttgcct cagcaccaag ttggccaatg ggctgcctgc tgggcgggca gtgggtgggg
     1801 acagcctaaa tggacaccca gttccaggct gtgccagcaa cccggttgcc tgtgaactga
     1861 aggcttttgt ggaagccacc tttcagagac agtttgtgct cacgctgagc gaactcaagc
     1921 gcctcttcaa cctgcacctg gcaggcctgc ccccgggcca tatactcttc agtggcgtct
     1981 cagaccgaat gctacaggat acggtgctgg ccgctggctg caagcagata ctggtgcctt
     2041 ttcccccaca gactgctgct tcccctgatg agcagaaggt atttgccctc tgggagtctg
     2101 gagacatgag tgaccagcat cggcaggttt tgcttgaaat attttccaaa aattaccgag
     2161 tacgtcggaa cttgatccag tctcggctga ctcaagaatg tggagaagag ctaagcaaac
     2221 aggaggtgga taaagtgcta aaggactgtt gtgtaagctg tggtggcatg tggtacctta
     2281 aggggacagt acaatcctga caataggagc aaactgctaa ctcgacgaat ccaagcccag
     2341 ggaaggatgg cagagccaca cgtagtcttg acttgcctca agacacagga agaggatctg
     2401 cctacctggt ggcctgtggc gctgcctggc ccagaggaca gaggggctca tgctcagcca
     2461 gtggcagggt cagcgtaagt gagagcgctc ccagcagaga ccagcgggcc ttctttgcag
     2521 tacaatttga aattcctgat gtattttgct tattgggttt cattctcata ataaagagag
     2581 tgtacactga catgggcagg attacgaaaa tcatggttta atattttcct cttttaaatg
     2641 aatgccaaca tagttaaaga tatgtttaca agatgaagag aaccttaagg tttttaatat
     2701 ttaaaatgcc tagaagccaa taattagtct tagagtttct ctctgctaag actcctgcca
     2761 attttattaa accaaattga acattttact gttgggtttc tgaggccact ttacctttat
     2821 agcagcctgc tttctgtagc ggtctcaact gtttacacaa accatagcaa aacattagat
     2881 taactccatc ttctcctaat gtttgcataa aggtgcaaac taaaccaggt aagtgtggaa
     2941 cagtatataa gcgaggttat cactttgatg taaaaaaatc tattttgtgt atttttaaaa
     3001 taaatgctaa cactaaactg gcgtcatagt gctgctgtcc tcagatagtt gcaggaagaa
     3061 aatgtgctct gtgcttccaa agcgttcagt actaaatgtc ctctgtgctt ccaaagcgtt
     3121 cagtactaaa tgtcctctgt gcttccaaag cgttcagtac taaatgtcct ctgtgcttcc
     3181 aaagcgttca gtactaaatg tcctctgtgc ttccaaagcg ttcagtacta aatgtcctct
     3241 gtgcttccaa agcgttcagt gctaaatgtc ctctgtgctt ccaaagcgtt cagtactaaa
     3301 tgtcctctgt gcttccaaag cgttcagtac taaatgtcct ctgtgcttcc aaagcgttca
     3361 gtactaaatg tcctctgtgc ttccaaagcg ttcagtgcta aatgtcctct gtgcttccaa
     3421 agcgttcagt actaaatgtc ctctgtgctt ccaaagcgtt cagtactaaa tgtcctctgt
     3481 gcttccaaag cgttcagtgc taaatgtcct ctgtgcttcc aaagcgttca gtactaaatg
     3541 tcctctgtgc ttccaaagcg ttcagtacta aatgtcctct gtgcttccaa agcgttcagt
     3601 actaaatgtc ctctgtgctt ccaaagcgtt cagtactaaa tgtcctctgt gcttccaaag
     3661 cgttcagtac taaatgtcct ctgtgcttcc aaagcgttca gtgctaaatg tcctctgtgc
     3721 ttccaaagcg ttcagtacta aatgtcctct gtgcttccaa agcgttcagt actaaatgtc
     3781 ctctgtgctt ccaaagcgtt cagtactaaa tgtcctctgt gcttccaaag cgttcagtac
     3841 taaatgtgct ctgtgcttcc aaagcgttca gtgctaaaga gagaaggggg ccatcacacc
     3901 gggtcatgtc catctgttgc tcaccttgac cttgtaagat tggtctgata gactggtctg
     3961 ctcacattgc tcaggccacc acacacatag ggactgagtg gccttagctg tgctgcagac
     4021 acggaagcgg aaggaaagaa ggatgggaag tgaacaccaa cttcttaaga agggaagtga
     4081 gctagcgcag caaactcctt ctgtgggagt tacatgaaaa tagtttttaa aaatagatat
     4141 taatgtactt cagacccaaa tatttaaaat caaaatgtaa taaagttttt ttttttaaac
     4201 taa
//
