LOCUS       NM_025945               2049 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus polymerase (RNA) III (DNA directed) polypeptide D
            (Polr3d), transcript variant 1, mRNA.
ACCESSION   NM_025945
VERSION     NM_025945.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Renaud,M., Praz,V., Vieu,E., Florens,L., Washburn,M.P., l'Hote,P.
            and Hernandez,N.
  TITLE     Gene duplication and neofunctionalization: POLR3G and POLR3GL
  JOURNAL   Genome Res 24 (1), 37-51 (2014)
   PUBMED   24107381
REFERENCE   3  (bases 1 to 2049)
  AUTHORS   Canella,D., Bernasconi,D., Gilardi,F., LeMartelot,G.,
            Migliavacca,E., Praz,V., Cousin,P., Delorenzi,M. and Hernandez,N.
  CONSRTM   CycliX Consortium
  TITLE     A multiplicity of factors contributes to selective RNA polymerase
            III occupancy of a subset of RNA polymerase III genes in mouse
            liver
  JOURNAL   Genome Res 22 (4), 666-680 (2012)
   PUBMED   22287103
REFERENCE   4  (bases 1 to 2049)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   5  (bases 1 to 2049)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122268.5, CD553533.1, AK013165.1, AW491400.1 and AI326828.1.
            
            On Aug 20, 2009 this sequence version replaced NM_025945.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016102.1, AK156587.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AC122268.5         67777-67794         c
            19-438              CD553533.1         1-420
            439-1491            AK013165.1         324-1376
            1492-2045           AW491400.1         13-566              c
            2046-2049           AI326828.1         4-7                 c
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 36.32 cM"
     gene            1..2049
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /note="polymerase (RNA) III (DNA directed) polypeptide D"
                     /db_xref="GeneID:67065"
                     /db_xref="MGI:MGI:1914315"
     exon            1..139
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            140..309
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1341
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /EC_number="2.7.7.6"
                     /note="BN51 (BHK21) temperature sensitivity complementing;
                     DNA-directed RNA polymerase III subunit C4; DNA-directed
                     RNA polymerase III subunit D"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC4"
                     /protein_id="NP_080221.3"
                     /db_xref="CCDS:CCDS27253.1"
                     /db_xref="GeneID:67065"
                     /db_xref="MGI:MGI:1914315"
                     /translation="MSEGNAAGEPSNPGGPRPLLSGGRGLIGRRPAPPLTPGRLPSIR
                     SRDLTLGGVKKKTFTPNIISRKIKEEPKEEVTMKKEKRERDRDRQREGHGRGRGRPEV
                     IQSHSIFEQGPAEMMKKKGNWDKTVDMSDMGPSHIINIKKEKRETDEETKQILRMLEK
                     DDFIDDPGLKNDTRNMPVQLPLAHSGWLFKEESEEPEAKPFSAGPKEEDMEVDVPAVK
                     VKEEPRDEEEEAKVKAPPRAARKTPGLPKDVSVAELLRELSLMKDEELLFLQLPDTLP
                     GQPPTQDIKPVKTEVQGEDGQMVVIKQEKDREARLAENACTLADLTEGQVGKLLIRKS
                     GKVQLLLGKVTLDVTMGTTCSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESL
                     LDHKHR"
     exon            310..353
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            354..505
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            506..630
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            631..799
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            800..1065
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1218
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..2049
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /note="hexamer: AATAAA"
     polyA_site      2007
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /note="major polyA site"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /note="hexamer: AATAAA"
     polyA_site      2045
                     /gene="Polr3d"
                     /gene_synonym="2810426M17Rik; 44kDa; BN51T; RPC4; TSBN51"
                     /note="major polyA site"
ORIGIN      
        1 gatgtcactt ccgccccagg gtaagatggc agcgcccggt ccttgccacg cagacttccg
       61 ccttgcccgg agacccgcgg cccgtggttg ctctagtcgg aggtgaggtt gtgatactcg
      121 gcgcagagcc tccacgcagg aaccatgtcg gaaggaaacg cggcgggcga gcccagcaat
      181 cctggagggc cgcgacccct tctctctggg ggccgggggc tgatcggccg gcggcccgca
      241 cccccgctta cgcccggtcg ccttccctcc atccgctccc gggacctcac ccttggcgga
      301 gtcaagaaga aaaccttcac cccaaatatc atcagtcgga agatcaaaga agagccaaaa
      361 gaagaagtga ctatgaagaa ggaaaagcgt gaacgggaca gggaccgcca gcgtgagggt
      421 cacggacggg gacgtggccg cccggaagtg atccagtccc attctatctt cgagcagggt
      481 ccggcagaaa tgatgaaaaa aaaagggaac tgggataaga ccgtggacat gtccgacatg
      541 ggaccttctc atatcatcaa catcaaaaaa gagaagagag aaacagacga agagaccaag
      601 cagatcctgc gcatgctgga gaaggatgac ttcatcgatg acccggggct aaagaatgac
      661 acacggaaca tgccagtgca gctgcctctg gctcattcag ggtggctttt taaggaggag
      721 agtgaagagc cagaggctaa gcctttttcg gctggcccca aggaagaaga tatggaggtg
      781 gacgtgcctg ccgtcaaagt gaaagaggag ccacgagatg aggaagagga agccaaggtg
      841 aaagcccctc caagagcagc ccggaagact cctggcctcc caaaggacgt gtcggtggcc
      901 gagctgctca gagagctgag cctcatgaag gatgaggagc tcctgttcct tcagctgcct
      961 gacaccctcc ccgggcagcc acccactcag gacatcaagc ctgtcaagac agaggtgcag
     1021 ggcgaggatg gacagatggt agtcataaag caggaaaaag accgggaagc caggctagca
     1081 gagaatgctt gtaccttggc tgacctgaca gagggtcaag tgggcaaact gctcatccgt
     1141 aagtcaggga aggtgcaact cctcctgggc aaggtgactc tagatgtgac catgggaacg
     1201 acctgttctt tcctgcagga actggtgtct gtgggccttg gagacagcag aacaggggag
     1261 atgacagtcc tgggacatgt gaagcacaaa cttgtatgtt ctcctgattt tgaatctctc
     1321 ttggatcaca aacaccggtg agatggacag atggaggagc acggtgactg tgcccacgac
     1381 cgcacatgct ctcaaataca cgcagcccag aagggcctct ttagcccacc cgttgtagcc
     1441 tttggctgta gtcccagttt cccccgacta gggccatgct gcccctcgcc ccagctgtga
     1501 atgggacctc acacagcctg ggtatggaag gcgcatcctc atctctggga acgtgaccct
     1561 gctatttatt ttttatttat gtcttaatcc tcaactgact gcattctccc agagtagaat
     1621 ggggcaggct tctttttggg gagccgtggg ctacggggat aggaagggaa ggggctcagg
     1681 caggtaagaa agaacaggga tgcgatggaa ctgaagactt aaaactcagt cccagctccc
     1741 tggtttctag ctctgtgtcc ttaaactaca taatctttct gagcctcata ttcctcaccc
     1801 gacagacatg aggacaatac ctacctcgca gggttggtgt gagcagcaca gggaatgttg
     1861 tagatgggaa ctccttgcac aagactggat atacattata taaggggtat gtgtgtgttt
     1921 gtatacacat aacatacata tgtatgtata tatacacatg tacataacca taggcgctca
     1981 ataaatgtta gttttccttt cctttgagtc tatttccaaa taaattaact ttatacttgg
     2041 aagaagtaa
//
