LOCUS       NM_027423               4997 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus polymerase (RNA) III (DNA directed) polypeptide B
            (Polr3b), mRNA.
ACCESSION   NM_027423 XM_125795
VERSION     NM_027423.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Malik,Y., Kulaberoglu,Y., Anver,S., Javidnia,S., Borland,G.,
            Rivera,R., Cranwell,S., Medelbekova,D., Svermova,T., Thomson,J.,
            Broughton,S., von der Haar,T., Selman,C., Tullet,J.M.A. and Alic,N.
  TITLE     Disruption of tRNA biogenesis enhances proteostatic resilience,
            improves later-life health, and promotes longevity
  JOURNAL   PLoS Biol 22 (10), e3002853 (2024)
   PUBMED   39436952
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Borland,G., Wilkie,S.E., Thomson,J., Wang,Z., Tullet,J.M.A.,
            Alic,N. and Selman,C.
  TITLE     Polr3b heterozygosity in mice induces both beneficial and
            deleterious effects on health during ageing with no effect on
            lifespan
  JOURNAL   Aging Cell 23 (5), e14141 (2024)
   PUBMED   38465473
  REMARK    GeneRIF: Polr3b heterozygosity in mice induces both beneficial and
            deleterious effects on health during ageing with no effect on
            lifespan.
REFERENCE   3  (bases 1 to 4997)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4997)
  AUTHORS   Choquet,K., Pinard,M., Yang,S., Moir,R.D., Poitras,C.,
            Dicaire,M.J., Sgarioto,N., Lariviere,R., Kleinman,C.L.,
            Willis,I.M., Gauthier,M.S., Coulombe,B. and Brais,B.
  TITLE     The leukodystrophy mutation Polr3b R103H causes homozygote mouse
            embryonic lethality and impairs RNA polymerase III biogenesis
  JOURNAL   Mol Brain 12 (1), 59 (2019)
   PUBMED   31221184
  REMARK    GeneRIF: missense mutations in Polr3a and Polr3b can variably
            impair mouse development and Pol III function.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4997)
  AUTHORS   Ma,L., Piirainen,S., Kulesskaya,N., Rauvala,H. and Tian,L.
  TITLE     Association of brain immune genes with social behavior of inbred
            mouse strains
  JOURNAL   J Neuroinflammation 12, 75 (2015)
   PUBMED   25895500
  REMARK    GeneRIF: The Polr3b in the hippocampus in the prefrontal cortex of
            the DBA/2 J strain were up-regulated.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4997)
  AUTHORS   Yee,N.S., Gong,W., Huang,Y., Lorent,K., Dolan,A.C., Maraia,R.J. and
            Pack,M.
  TITLE     Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of
            Subunit Rpc11 and disrupts zebrafish digestive development
  JOURNAL   PLoS Biol 5 (11), e312 (2007)
   PUBMED   18044988
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK165886.1 and BC068143.1.
            
            On Jun 28, 2018 this sequence version replaced NM_027423.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068143.1, SRR1660825.184404.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               AK165886.1         6-144
            140-4997            BC068143.1         1-4858
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 41.58 cM"
     gene            1..4997
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /note="polymerase (RNA) III (DNA directed) polypeptide B"
                     /db_xref="GeneID:70428"
                     /db_xref="MGI:MGI:1917678"
     exon            1..241
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..3571
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /EC_number="2.7.7.6"
                     /note="RNA polymerase III subunit C2; DNA-directed RNA
                     polymerase III subunit B; DNA-directed RNA polymerase III
                     127.6 kDa polypeptide; RNA polymerase III subunit RPC2
                     homolog"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC2"
                     /protein_id="NP_081699.1"
                     /db_xref="CCDS:CCDS24081.1"
                     /db_xref="GeneID:70428"
                     /db_xref="MGI:MGI:1917678"
                     /translation="MDVLAEEFGSLTPEQLTAPIPTVEEKWRLLPAFLKVKGLVKQHI
                     DSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHEC
                     RLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNE
                     CPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAV
                     KQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQ
                     IFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIY
                     TAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVI
                     PKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALG
                     MMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMED
                     GPIIKLAGNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVSTFRLMRRAG
                     YINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDF
                     LHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPR
                     NTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNAT
                     VAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAA
                     TRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPI
                     TYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQE
                     DMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDV
                     CEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAV
                     LTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGY
                     SGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLAKYNE"
     exon            242..274
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            275..331
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            332..396
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            397..472
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            473..573
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            574..665
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..783
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            784..892
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1015
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1135
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1270
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1432
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1633
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1796
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1950
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2025
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2124
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2252
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2462
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2621
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2739
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2882
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..2986
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3153
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3267
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3441
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..4969
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4943..4948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /note="hexamer: AATAAA"
     polyA_site      4969
                     /gene="Polr3b"
                     /gene_synonym="2700078H01Rik; A330032P03Rik; C128; RPC2"
                     /note="major polyA site"
ORIGIN      
        1 ctccgcaggt taggccctga ccaggcggaa ccttttgaaa cgcctccccg taaacaagcg
       61 ccacggggac accggaggag aacgcacacc actctccgcg tgctacttgc ttggagccgc
      121 acgggcggat gtgaacattc tgccgacttc ttcctctacc gtgaatggaa tggatgttct
      181 agccgaggag ttcgggagcc tgactccgga gcagctgact gcgccgatcc cgactgtgga
      241 ggaaaaatgg aggctgcttc cagcattttt aaaggttaaa ggcctggtga aacagcacat
      301 agactccttt aactatttca ttaatgtaga gataaagaag ataatgaaag ccaatgaaaa
      361 ggttacaagt gatgctgacc ccatgtggta cttaaaatac cttaatatct atgtcggact
      421 tcctgatgtt gaagaaagct tcaatgtaac tagaccagtg tcccctcatg agtgccgtct
      481 gagggacatg acgtactccg ccccaatcac agtggacatt gagtataccc gaggcagcca
      541 gaggataatc cgcaacgcct tacccattgg cagaatgccc atcatgctcc gaagctcgaa
      601 ctgtgttcta acagggaaaa cgccggcaga gtttgccaaa ctgaatgagt gtccactaga
      661 cccaggtggc tacttcattg ttaaaggagt ggaaaaagtc attcttatcc aagagcagct
      721 gtccaagaac aggatcattg tggaggccga caggaaagga gccgttggag cctcagttac
      781 cagctccacc catgagaaga aaagcagaac caacatggcc gtgaaacagg gtcgattcta
      841 tctgaggcac aacaccctgt ctgaagatat ccccattgtc atcatattta aggccatggg
      901 tgtggagagt gaccaggaaa tcgtgcagat gattggaaca gaggagcacg tgatggctgc
      961 atttgggccc agcttggagg agtgccagaa agctcagatc ttcacccaga tgcaggcatt
     1021 gaagtatata gggaataaag taagaagaca aagaatgtgg ggaggtggac caaagaaaac
     1081 caaaatagaa gaagcaagag aactcctggc ttctaccatt ctgacccacg taccagtgaa
     1141 ggaattcaat ttccgagcta agtgtatcta cactgcagtg atggtgcgaa gggtgatcct
     1201 ggcccaagga gataacaagg tcgatgacag agactattat ggcaacaaac gactggaatt
     1261 ggcaggacag ctcttatctc ttctttttga agatttgttt aaaaagttta attcagaaat
     1321 gaagaagatt gcagaccaag tgattcctaa acagagagca gcccagtttg acgtcgttaa
     1381 acacatgcgc caagaccaga ttaccaacgg catggtcaat gccatctcca cgggaaattg
     1441 gtctctgaag agatttaaga tggaccgaca gggtgtgacc caggtgctgt ctcgcttgtc
     1501 atatatatct gcactcggca tgatgacaag aatctcttcc cagtttgaaa agacgaggaa
     1561 agtgagtggc cctcgttctc tgcagccgtc tcagtgggga atgctctgtc cctccgacac
     1621 acctgaaggg gaggcctgtg gtttggttaa gaacttggcc ctcatgacac atataacaac
     1681 tgacatggaa gatggaccca ttattaagtt agctgggaac ttgggagtag aagatgtgaa
     1741 tttattatgt ggagaagagc tctcttaccc aaatgtgttt cttgtcttcc tgaatggtaa
     1801 catccttggt gtcattcgag accataaaaa gcttgtcagc acattccggc tgatgcgaag
     1861 agcaggctat atcaatgaat ttgtttctat ctccacaaac cttacggatc gctgtgttta
     1921 tatttcctct gatggcgggc gcttgtgcag gccttacata attgttaaga aacaaaagcc
     1981 agctgtcaca aataagcaca tggaagagtt ggctcaaggc tacaggaatt ttgaagattt
     2041 cttacatgaa agtctagttg aatacttaga tgtgaatgaa gaaaatgact gtaacattgc
     2101 gctctacgaa catacaatta ataaagacac cacccacttg gagattgaac ccttcactct
     2161 gcttggtgtt tgtgccggcc tgatcccata ccctcatcac aaccagtctc caagaaacac
     2221 ctaccagtgt gctatgggca agcaagccat gggtaccatt ggctacaatc aacggaacag
     2281 aattgataca cttatgtatc tactagcata tccacaaaag cccatggtta aaacaaaaac
     2341 aatcgagttg atagattttg agaagttgcc ggctggccag aacgcaactg ttgccgtgat
     2401 gagctacagc ggctatgaca ttgaggacgc gctggttttg aacaaggcct ctctggacag
     2461 aggttttggg cgttgtcttg tgtataaaaa tgctaagtgc accttgaaac gatacaccaa
     2521 tcagaccttt gataaggtga tgggtcccat gttggatgct gctaccagaa aacccatctg
     2581 gcgacatgaa attttagatg cagatggcat ttgttctcca ggtgagaaag tggaaaacaa
     2641 acaggtgctt gtaaacaagt ccatgcccac agtgacacag attcctttgg aagggagcaa
     2701 cgtgccacag caaccacagt ataaagatgt gcctatcacg tacaaaggtg cgacagactc
     2761 ctacatcgag aaagtgatga tctcttccaa cgccgaggat gctttcctca tcaagatgct
     2821 cctgagacag acgcggcgcc cagagattgg agacaaattc agcagtcgcc atggacaaaa
     2881 gggtgtctgt ggcctgatcg tcccccagga agacatgccg ttctgtgatt ctggcatctg
     2941 tcccgacatc ataatgaacc cacacggctt cccatcaaga atgacggttg gaaagttaat
     3001 tgagctgctg gctggcaagg caggcgtgtt agacggcagg ttccactacg gcactgcctt
     3061 tggagggagt aaagtgaagg atgtgtgtga agatctcgtt cgccatggtt acaactacct
     3121 ggggaaggac tacgtcacct caggcatcac aggtgagccc ttagaagcat acatctattt
     3181 tggacctgtg tattatcaga aactgaaaca catggtgctg gataaaatgc acgcccgggc
     3241 ccgaggacca cgagctgtcc ttaccaggca gcccactgaa gggcggtcgc gcgatggtgg
     3301 cttgcgactt ggagaaatgg aacgcgactg tctaatcggc tatggagcga gtatgctttt
     3361 gctggagaga ctaatgatct caagtgatgc ctttgaggtt gacgtctgcg ggcagtgtgg
     3421 actcctggga tattctggct ggtgccacta ctgcaaatct tcgtgccacg tgtcctccct
     3481 ccgcatcccg tatgcctgca agctgctctt ccaggagtta cagtccatga acatcatccc
     3541 caggctgaaa ctggccaagt acaacgagta aggctgtgcg gtgctcacga gggtgcagtc
     3601 ggccgacggc ctgtacagcc tgaggcaggg ggctctcggt gacttctgtg ctcattctgc
     3661 ttcacggagg aggcagagga ggaggaggaa gaagaggaag aaggagagga ggaggaagag
     3721 aatgaaggag aggaggaaga ggagcagcag cagcagctgt ctgaggtcca gaggctggct
     3781 tgacaccctt gatctggagc ctgaagccat aggaggtgct gggccccgca cagcactgag
     3841 tggagtcaca cacagatgga cacaagctag agagagtacc aacgcctatt aattttgttg
     3901 tttgtttgtt tgtgttgagt gggagtctca ctctatccca ggtgggattt gaactcacct
     3961 caattctcct gcctttgtct cccacaagtg ctggaatcat ggatatgagc caagcatatc
     4021 tggtttgttt atgttattat gttttaaact gtacttgtta gaaaaacctt cttcctttaa
     4081 atttcctctg aggtcttcta gctaacagaa tcctgggttc tcatcagatg tggaggaggt
     4141 aaagaattgt ttgaattggg actgagaagt tagctcagtc aagggcctgt tgcataagta
     4201 tgacctgagt tcacccccca acaacccatg taagggctgg gtgtggcacc tagcacccct
     4261 gtaaaagtca gatactgagg gggtgggggt ggggagcaga cagactgacc cctggcgctt
     4321 tctgaccagc tggcataacc agtcagtgca attcaggttc aatgagggac ccctgtgtca
     4381 agggctagtg tgaacagtga ctgaggagga ccccagtgtc cctctggcct ccacacacac
     4441 atccaaagaa tgggtagatg tgtgggcaca acttttgctg aggtgggaag aagtaaactt
     4501 tctctcagag gacagcaagt gacatttgtg actctgaaag accagcagtc acagtctgct
     4561 gtcacattgt gagtcagagc ttgctcagac atcgatcttg atggaatgtg atctgtgaag
     4621 tctacctttt aagactctcc tcctacttct tttgaacctg caggatacct aggtctaatc
     4681 catgggcccc tggtcactca tttcctacct ttccattgct accagaaagt tagctgtcca
     4741 ctctctcagt ttgttcttaa ctgttgtgcc ctggctccag ataaatgttt atatggagaa
     4801 gtgttcagag agagagtgtg ccccagcagc attgagtgtg cggatgaggc tgaggaggga
     4861 gtgcgaggac tttgcagatc tcactccagg ctcatccgga tgagtcacga cttaaagact
     4921 caaccctgtt tgttttcttc acaataaaga tgtggcagtc agaacggtaa aaaaaaaaaa
     4981 aaaaaaaaaa aaaaaaa
//
