LOCUS       NM_023127                494 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Mus musculus polymerase (RNA) II (DNA directed) polypeptide K
            (Polr2k), transcript variant 2, mRNA.
ACCESSION   NM_023127
VERSION     NM_023127.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   2  (bases 1 to 494)
  AUTHORS   Rother,F., Shmidt,T., Popova,E., Krivokharchenko,A., Hugel,S.,
            Vilianovich,L., Ridders,M., Tenner,K., Alenina,N., Kohler,M.,
            Hartmann,E. and Bader,M.
  TITLE     Importin alpha7 is essential for zygotic genome activation and
            early mouse development
  JOURNAL   PLoS One 6 (3), e18310 (2011)
   PUBMED   21479251
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 494)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   4  (bases 1 to 494)
  AUTHORS   Penkowa,M., Camats,J., Giralt,M., Molinero,A., Hernandez,J.,
            Carrasco,J., Campbell,I.L. and Hidalgo,J.
  TITLE     Metallothionein-I overexpression alters brain inflammation and
            stimulates brain repair in transgenic mice with astrocyte-targeted
            interleukin-6 expression
  JOURNAL   Glia 42 (3), 287-306 (2003)
   PUBMED   12673834
  REMARK    GeneRIF: Metallothionein-I was upregulated by a cryolesion to a
            higher extent in GFAP-IL-6 transgenic mice, suggesting that it
            could be related to the neuroprotection afforded by the transgenic
            expression of IL-6.
REFERENCE   5  (bases 1 to 494)
  AUTHORS   Remondelli,P. and Leone,A.
  TITLE     Interactions of the zinc-regulated factor (ZiRF1) with the mouse
            metallothionein Ia promoter
  JOURNAL   Biochem J 323 (Pt 1) (Pt 1), 79-85 (1997)
   PUBMED   9173905
REFERENCE   6  (bases 1 to 494)
  AUTHORS   Xu,C.
  TITLE     cDNA cloning of a mouse factor that activates transcription from a
            metal response element of the mouse metallothionein-I gene in yeast
  JOURNAL   DNA Cell Biol 12 (6), 517-525 (1993)
   PUBMED   8329119
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CR516049.1, AK003023.1, AK008136.1 and BY646564.1.
            
            On Oct 23, 2013 this sequence version replaced NM_023127.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks
            part of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (b) has a shorter N-terminus, compared to isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR516049.1, CO797967.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                CR516049.1         462-482             c
            22-425              AK003023.1         4-407
            426-489             AK008136.1         515-578
            490-494             BY646564.1         416-420
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 14.46 cM"
     gene            1..494
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="polymerase (RNA) II (DNA directed) polypeptide K"
                     /db_xref="GeneID:17749"
                     /db_xref="MGI:MGI:102725"
     exon            1..72
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     exon            73..142
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..258
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /EC_number="2.7.7.6"
                     /note="isoform b is encoded by transcript variant 2;
                     DNA-directed RNA polymerases I, II, and III subunit
                     RPABC4; metallothionein-I gene transcription activator"
                     /codon_start=1
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit RPABC4 isoform b"
                     /protein_id="NP_075616.2"
                     /db_xref="CCDS:CCDS56980.1"
                     /db_xref="GeneID:17749"
                     /db_xref="MGI:MGI:102725"
                     /translation="MDAQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIM
                     YKKRTKRLVVFDAR"
     exon            143..235
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     exon            236..494
                     /gene="Polr2k"
                     /gene_synonym="ABC10-alpha; MafY; Mt1a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagttggc gaggcagcgc cggaaaaagc ttttgcgtgc tcagagaagg gacgcttctt
       61 ccagctgtgg aggcactaac aatggacgct cagaaagatg ttcaaccacc aaagcagcag
      121 ccaatgatat atatttgtgg agagtgtcac accgaaaatg agataaagtc cagggatccc
      181 atcagatgca gagaatgtgg atacagaata atgtacaaga aaaggactaa aagattggtg
      241 gtttttgatg ctcgatgaaa tgtgggagtt cagaagtatc ttcattcata catttgcctc
      301 attgatgttt ctctttaatg tatagcattt ggcttagttt acttaatgaa aacaactgta
      361 tgcctatgat ttcatttaga aatacaattg tattttaata ctttgtataa aggaaatttt
      421 ggttcaatat atggcttaat ttattgcttt tgtaatccgt cacacagtga agattaaaag
      481 tgtttcctca aacc
//
