LOCUS       NM_145822               2248 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus RNA polymerase I subunit G (Polr1g), mRNA.
ACCESSION   NM_145822
VERSION     NM_145822.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   McNamar,R., Freeman,E., Baylor,K.N., Fakhouri,A.M., Huang,S.,
            Knutson,B.A. and Rothblum,L.I.
  TITLE     PAF49: An RNA Polymerase I subunit essential for rDNA transcription
            and stabilization of PAF53
  JOURNAL   J Biol Chem 299 (8), 104951 (2023)
   PUBMED   37356716
  REMARK    GeneRIF: PAF49: An RNA Polymerase I subunit essential for rDNA
            transcription and stabilization of PAF53.
REFERENCE   3  (bases 1 to 2248)
  AUTHORS   Werber,M., Wittler,L., Timmermann,B., Grote,P. and Herrmann,B.G.
  TITLE     The tissue-specific transcriptomic landscape of the mid-gestational
            mouse embryo
  JOURNAL   Development 141 (11), 2325-2330 (2014)
   PUBMED   24803591
REFERENCE   4  (bases 1 to 2248)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2248)
  AUTHORS   Penrod,Y., Rothblum,K. and Rothblum,L.I.
  TITLE     Characterization of the interactions of mammalian RNA polymerase I
            associated proteins PAF53 and PAF49
  JOURNAL   Biochemistry 51 (33), 6519-6526 (2012)
   PUBMED   22849406
  REMARK    GeneRIF: yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric
            complexes interact through species-specific sequence elements that
            appear to assume similar structural motifs.
REFERENCE   6  (bases 1 to 2248)
  AUTHORS   Ushijima,R., Matsuyama,T., Nagata,I. and Yamamoto,K.
  TITLE     Nucleolar targeting of proteins by the tandem array of basic amino
            acid stretches identified in the RNA polymerase I-associated factor
            PAF49
  JOURNAL   Biochem Biophys Res Commun 369 (4), 1017-1021 (2008)
   PUBMED   18329376
  REMARK    GeneRIF: Serial deletion analysis revealed that amino acids 199-338
            were necessary for the nucleolar localization of PAF49.
REFERENCE   7  (bases 1 to 2248)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 2248)
  AUTHORS   Yamamoto,K., Yamamoto,M., Hanada,K., Nogi,Y., Matsuyama,T. and
            Muramatsu,M.
  TITLE     Multiple protein-protein interactions by RNA polymerase
            I-associated factor PAF49 and role of PAF49 in rRNA transcription
  JOURNAL   Mol Cell Biol 24 (14), 6338-6349 (2004)
   PUBMED   15226435
  REMARK    GeneRIF: These results strongly suggest that RNA polymerase I (Pol
            I)-associated factor PAF49/ASE-1 plays an important role in rRNA
            transcription.
REFERENCE   9  (bases 1 to 2248)
  AUTHORS   Uinuk-Ool,T., Mayer,W.E., Sato,A., Dongak,R., Cooper,M.D. and
            Klein,J.
  TITLE     Lamprey lymphocyte-like cells express homologs of genes involved in
            immunologically relevant activities of mammalian lymphocytes
  JOURNAL   Proc Natl Acad Sci U S A 99 (22), 14356-14361 (2002)
   PUBMED   12391333
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148988.5.
            
            On Feb 2, 2010 this sequence version replaced NM_145822.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.108132.1,
                                           SRR7345562.3970080.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AC148988.5         151343-151438       c
            97-238              AC148988.5         151045-151186       c
            239-2248            AC148988.5         147962-149971       c
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.62 cM"
     gene            1..2248
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /note="RNA polymerase I subunit G"
                     /db_xref="GeneID:70333"
                     /db_xref="MGI:MGI:1917583"
     exon            1..96
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1274
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /note="CD3 epsilon associated protein; antisense to Ercc1;
                     CD3E antigen, epsilon polypeptide associated protein; RNA
                     polymerase I-associated factor PAF49; A34.5;
                     CD3E-associated protein; anti-sense to ERCC-1 protein;
                     CD3-epsilon-associated protein; DNA-directed RNA
                     polymerase I subunit G"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase I subunit RPA34"
                     /protein_id="NP_665821.1"
                     /db_xref="CCDS:CCDS39798.1"
                     /db_xref="GeneID:70333"
                     /db_xref="MGI:MGI:1917583"
                     /translation="MAGTQACSATRFSCPPHFTEMSPDSEPSRFSLEALTGPDTELWL
                     IQAPADFAPQCLNGRRVPLSGSKTVKGKLDGKKHRYRVFTSSPQAREATLLASSSEAG
                     GRLTCAPAPSGSLRIMEGPQEYLLSRVPLQLIPTSLPPQIPAGLRPRFSAFGGSPPVT
                     GPGSASALRSPTSGKRKSTRKGTDASSDTQEAVNRHGAMEVKTALGNLGVSVKKRKRY
                     FMQEEMEAKTMEPVAELPVPSATSSKKRKKSKGTETSQVEHTEPVAQTEPPEGTFLFP
                     TKKRKRQKEADGTEEVDGIVADSQPQVIVEAQEETILLSPTKKRRKEKRQNLGMEAEM
                     GPPGVLMVTEHSEHGLQAEVALVSPKKTKKKKGKRVGETEAALPDDFEPQGALAPSKK
                     KERGARL"
     exon            97..238
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /inference="alignment:Splign:2.1.0"
     exon            239..2248
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1866..1871
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /note="hexamer: ATTAAA"
     polyA_site      1901
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
     regulatory      2229..2234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /note="hexamer: AATAAA"
     polyA_site      2248
                     /gene="Polr1g"
                     /gene_synonym="1190028F09; 2610103M17Rik; ASE-1; Ase1;
                     CAST; Cd3eap; PAF49"
                     /note="major polyA site"
ORIGIN      
        1 gcggctccac cccttggatc cacgtggtgg gcagcgttcc gcgcaggtcc tgcgtgggcc
       61 cctccggagc tggaatggcg ggtacccagg cctgcagtgc cactcggttc tcctgccctc
      121 cccacttcac ggagatgtcg ccagactcgg aaccctcccg tttctccttg gaggcgctta
      181 caggtccaga tacagaactg tggcttatcc aagcccctgc agacttcgcc ccacaatgtc
      241 tcaatgggcg gcgtgtacct ctttctggct ccaagactgt taagggcaaa ctggatggca
      301 agaagcaccg ataccgggta tttaccagca gtccccaggc ccgagaagcc accttgctcg
      361 catcttcatc agaggcaggt ggcagactca cctgcgcccc ggcccccagt gggagcttaa
      421 ggatcatgga gggtcctcaa gaatatcttc tctctcgggt ccccctgcag ctgatcccta
      481 caagtctgcc acctcagatc cctgctggcc tgcggcctcg cttctccgcc tttggcggca
      541 gccctcccgt cacggggcca ggttcagcct cagctctgag gtcaccaact tcagggaaga
      601 ggaaaagtac aagaaagggt acagatgcct cctcagacac tcaggaggct gtgaacaggc
      661 acggggccat ggaagtaaaa acagccttgg gtaacctagg agtgagtgtg aagaagagaa
      721 agaggtattt catgcaggag gagatggaag ccaagacaat ggagcccgtg gcagagctgc
      781 cagtcccatc tgccaccagc agcaagaaga ggaagaagtc caaggggaca gagacatccc
      841 aggtggagca cacagaacct gtggctcaaa cagagcctcc tgaggggacc ttcctgttcc
      901 ccaccaaaaa gaggaagagg cagaaggaag ctgatgggac agaggaagtg gatggtatag
      961 tggctgactc tcagccacag gtcattgtgg aagcccagga ggaaaccatc ctcctgtccc
     1021 ccacaaagaa gaggagaaag gaaaagagac agaatttggg gatggaggca gagatggggc
     1081 cccctggagt actcatggtg accgaacatt cagaacatgg gctccaggct gaagtagctc
     1141 ttgtgtcccc caagaagaca aagaagaaaa agggaaagcg ggtgggtgag acagaggctg
     1201 cacttccaga tgactttgag ccccaggggg ctctagcacc cagcaagaaa aaagagagag
     1261 gcgcacgcct ttaatcccag cactgggcag gcagaggcag gcagatttct gagttcgagg
     1321 ccagcctggt ctacaaagag ttctaggaca gccagggcta cacagagaaa ccctgtctca
     1381 aaaaaccaaa aaaaaaaaaa aaaaaagaaa gaaagaaaaa agagaggaca gaaagcaatg
     1441 gagcccgggt ctaaggtgat tgacccaaga caatccgagg aaccagagcc caaggccagt
     1501 caaagatcct ccaagaagaa aaagagggat caggaaactg aggtgcaaga caccattccc
     1561 caatgagaga tgtcttccac ggacaggaga agcagcagag gagacttgca caggagccgt
     1621 aataacaccc tggggaaggg gagaaggagg gactcgccag gctgaggtca gaaaccagct
     1681 gtatagacac tccctgcccc tcccccaagc aaacacgcag agccactgga gcctgggcat
     1741 ggaaaattct ttattgtgac actgggccta ggtggcagca ggtcatcgag gcactttgag
     1801 gaagggttcg tgtagtactt caaagagcct gcgggcctgg gggcaagggg agaagagaac
     1861 ctgtcattaa agctgtcgct ggcccgcctg tttggaatgg ggttctgcct cttattctcc
     1921 tggctgagga gcttcgggtg tctgggttct acatgaggct ggcccggagg tggatggaaa
     1981 tggacctaaa aactagtaca tcgctgggcg tggtggcgca cgcctttgat cccagcattc
     2041 gggaggcaaa ggcaggtgga tttcggagtt caaggccagc ctggtctacc cagaaaccct
     2101 gtctcaaaaa acaaaaacaa aaactagtac atcgctgggc ggtggtggtg cacacctttg
     2161 atcccagcac tcgggaagct gaggcaggag gatcggaagt gcaaggccag cctcagctac
     2221 attaagtaaa taaaacaaac caacaaaa
//
