LOCUS       NM_009086               4017 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus polymerase (RNA) I polypeptide B (Polr1b), mRNA.
ACCESSION   NM_009086
VERSION     NM_009086.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Liu,H., Huang,T., Li,M., Li,M., Zhang,C., Jiang,J., Yu,X., Yin,Y.,
            Zhang,F., Lu,G., Luo,M.C., Zhang,L.R., Li,J., Liu,K. and Chen,Z.J.
  TITLE     SCRE serves as a unique synaptonemal complex fastener and is
            essential for progression of meiosis prophase I in mice
  JOURNAL   Nucleic Acids Res 47 (11), 5670-5683 (2019)
   PUBMED   30949703
REFERENCE   3  (bases 1 to 4017)
  AUTHORS   Zhang,Q., Ji,S.Y., Busayavalasa,K. and Yu,C.
  TITLE     SPO16 binds SHOC1 to promote homologous recombination and
            crossing-over in meiotic prophase I
  JOURNAL   Sci Adv 5 (1), eaau9780 (2019)
   PUBMED   30746471
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4017)
  AUTHORS   Zhang,Q., Shao,J., Fan,H.Y. and Yu,C.
  TITLE     Evolutionarily-conserved MZIP2 is essential for crossover formation
            in mammalian meiosis
  JOURNAL   Commun Biol 1, 147 (2018)
   PUBMED   30272023
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4017)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4017)
  AUTHORS   Yuan,X., Zhou,Y., Casanova,E., Chai,M., Kiss,E., Grone,H.J.,
            Schutz,G. and Grummt,I.
  TITLE     Genetic inactivation of the transcription factor TIF-IA leads to
            nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis
  JOURNAL   Mol Cell 19 (1), 77-87 (2005)
   PUBMED   15989966
REFERENCE   7  (bases 1 to 4017)
  AUTHORS   Seither,P., Iben,S., Thiry,M. and Grummt,I.
  TITLE     PAF67, a novel protein that is associated with the
            initiation-competent form of RNA polymerase I
  JOURNAL   Biol Chem 382 (8), 1163-1170 (2001)
   PUBMED   11592397
REFERENCE   8  (bases 1 to 4017)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   9  (bases 1 to 4017)
  AUTHORS   Seither,P., Coy,J.F., Pouska,A. and Grummt,I.
  TITLE     Molecular cloning and characterization of the cDNA encoding the
            largest subunit of mouse RNA polymerase I
  JOURNAL   Mol Gen Genet 255 (2), 180-186 (1997)
   PUBMED   9236775
REFERENCE   10 (bases 1 to 4017)
  AUTHORS   Seither,P. and Grummt,I.
  TITLE     Molecular cloning of RPA2, the gene encoding the second largest
            subunit of mouse RNA polymerase I
  JOURNAL   Genomics 37 (1), 135-139 (1996)
   PUBMED   8921381
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB860575.1, AK143501.1 and U58280.1.
            
            On Mar 13, 2009 this sequence version replaced NM_009086.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U58280.1, AK144616.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                BB860575.1         7-70
            65-3997             AK143501.1         2-3934
            3998-4017           U58280.1           3959-3978
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.68 cM"
     gene            1..4017
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /note="polymerase (RNA) I polypeptide B"
                     /db_xref="GeneID:20017"
                     /db_xref="MGI:MGI:108014"
     exon            1..268
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..3499
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /EC_number="2.7.7.6"
                     /note="RNA polymerase 1-2, 128 kDa subunit; DNA-directed
                     RNA polymerase I 135kDa polypeptide; RNA polymerase I
                     subunit 2; DNA-directed RNA polymerase I 135 kDa
                     polypeptide"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase I subunit RPA2"
                     /protein_id="NP_033112.2"
                     /db_xref="CCDS:CCDS16720.1"
                     /db_xref="GeneID:20017"
                     /db_xref="MGI:MGI:108014"
                     /translation="MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVD
                     SFNYAALEGLSHAVQAIPPFEFAFKDERISLTIVDAVISPPSVPKGTICKDLNVYPAE
                     CRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLYNLPPRVLIEHH
                     EEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGVSMRCVREE
                     HSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDS
                     FFRNSVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICI
                     HLQSNTDKFYLLCLMTRKLFALARGECMDDNPDSLVNQEVLSPGQLFLMFLKEKMENW
                     LVSIKIVLDKRAQKANVSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFLQD
                     SGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLLPESWGFLCPVHTPDGAPC
                     GLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDTAPCRPYSDCYPVLLDGVMVGWV
                     DKDLAPEVADTLRRFKVLREKRIPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQ
                     NLELGREELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQS
                     PRNMYQCQMGKQTMGFPLLTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTN
                     AIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGEDNLVFGVKP
                     GDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVYYKSKENCVVDNIKVCS
                     NDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDIL
                     FNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYN
                     FYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRN
                     VQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSW
                     SAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVI"
     exon            269..436
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..583
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            584..716
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            717..853
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1077
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1249
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1421
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1703
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1837
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..2008
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2165
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2362
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2616
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..3998
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3977..3982
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /note="hexamer: AATAAA"
     polyA_site      3998
                     /gene="Polr1b"
                     /gene_synonym="128kDa; D630020H17Rik; RPA116; RPA135;
                     RPA2; Rpo1-2"
                     /note="major polyA site"
ORIGIN      
        1 ggggtagggt ggctgtgggg ttggtccggc cattccagcc agccacgagt agcgtcctag
       61 ctccggtctc ccgtccggga ctggcagaag catggatgtc gacggccggt ggcggaacct
      121 gcccagcggg cccagcctaa agcatttaac cgacccctcg tacgggattc ccccagagca
      181 gcaaaaggca gctttgcagg acctgacgcg ggcgcacgtg gactccttca actacgcggc
      241 gctggagggg ctgagccacg cggtgcaggc catacctccc tttgaatttg ctttcaaaga
      301 tgagcgcata tctcttacca ttgtggatgc tgtcatcagt ccaccctcag tgcccaaagg
      361 gaccatctgc aaagatctca acgtttatcc agctgagtgt cgtggccgaa agagcaccta
      421 ccgagggagg ctgacggctg atatcagctg ggccgtgaat ggagtcccca aagggatcat
      481 taaacagttt ctcggctatg ttcccatcat ggtgaagtcc aagctttgca acttatacaa
      541 ccttcctcct cgagtcctca ttgagcacca tgaggaggca gaggaaatgg gcggttattt
      601 tataatcaat ggcatagaaa aagtcatccg catgttgatt gtgccccgga gaaatttccc
      661 catcgccatg gtaaggccga aatggaaaag cagaggactc ggctacactc agtttggggt
      721 ctccatgcgc tgtgtgagag aagagcactc cgccgtgaac atgaacctgc actatgtgga
      781 gaacggcacg gttatgttaa actttattta ccgcaaagag ctctttttcc ttcctttggg
      841 atttgcactt aaggcactcg tgagcttttc tgattatcag attttccagg agcttatcaa
      901 gggcaaagag gaggactctt tctttaggaa ttctgtttct cagatgctgc ggattgtcat
      961 agaagagggc tgtcactcac agaagcaggt tcttaactat ctgggagaat gcttccgagt
     1021 gaagctcagc cttcctgatt ggtaccctaa cgtggaagct gccgagttcc tgcttaacca
     1081 gtgcatctgc atccacctgc agtccaacac tgacaagttc tacctgctct gcctcatgac
     1141 ccggaagctg ttcgctttag ccagagggga gtgcatggac gacaaccctg atagcctagt
     1201 gaaccaagag gtcctcagcc ctgggcagct cttcctgatg tttctgaagg aaaaaatgga
     1261 aaactggcta gtgtctatta aaatagtttt agataagagg gctcagaagg ccaatgtttc
     1321 cataaacaac gaaaatttga tgaagatttt tagtatggga acagagctaa caagaccatt
     1381 tgaatatctt cttgctactg ggaatctgcg ttctaaaaca ggtctcggct tcctgcaaga
     1441 ttctggcctg tgtgttgtgg ctgacaagct gaacttcctt cgctacctct cccatttccg
     1501 ctgtgtgcac agaggggctg cctttgccaa gatgaggacc accaccgtgc gcaggctgct
     1561 gccagaatcc tggggcttcc tctgccctgt gcacactcca gacggggcgc cttgtgggct
     1621 gttgaaccac ttgactgccg tgtgtgaggt tgttaccaag tttgtgtaca cggcatctat
     1681 tccagccttg ctctgtggct taggagtcac tcctgttgat acagcgccat gtcgaccgta
     1741 cagtgactgc taccctgtcc tgctggatgg cgtcatggtg ggctgggtgg ataaggacct
     1801 tgctcctgaa gttgcagaca ctctccgtcg attcaaggtg ttgagagaaa aaagaattcc
     1861 tccctggatg gaggtggccc tgattcccat gacaggaaaa ccaagtctgt acccaggtct
     1921 gttcctcttt accactccct gcaggctggt gaggcctgtg cagaacttgg aactgggcag
     1981 agaagagctc attggaacca tggaacagct cttcatgaac gtcgccatct ttgaggacga
     2041 ggtgtttggt ggcatctcca cacaccagga gctcttccct cacagcctgc tgagcgtgat
     2101 cgccaacttc atccccttct cggatcacaa ccagagtcct cggaacatgt accagtgcca
     2161 gatgggtaag cagaccatgg gctttccgct gctcacttac caaaaccgat cagataataa
     2221 actctatcga ctccaaaccc ctcagagccc tctagtgaga ccgtgcatgt atgatttcta
     2281 tgacatggac aactatccga tcgggacaaa cgccattgta gctgtgatct cctacactgg
     2341 ctatgatatg gaggacgcca tgattgtcaa caaggcttcc tgggaacgag gcttcgctca
     2401 tggaagcgtc tacaagtctg agttcataga cctctcagag aagtttaagc agggagagga
     2461 taacctggtg ttcggggtca agcctggtga ccctcgggtg atgcagaagc tggacgatga
     2521 tgggttgcca ttcataggcg caaagctgga gtatggcgac ccttactaca gctacctcaa
     2581 ccttaacact ggggaaggct tcgtggttta ctacaagagt aaagaaaact gtgttgtgga
     2641 caacatcaaa gtgtgtagta acgacatggg cagtgggaag ttcaagtgta tctgcatcac
     2701 agtccgcatc ccccggaacc caaccattgg agataagttt gccagccgac atggtcagaa
     2761 gggcattttg agcagattgt ggccagccga ggacatgcct ttcactgaga gtggcatgat
     2821 gccggacatt ctgtttaatc ctcacgggtt tccctcccgt atgaccatag gtatgttaat
     2881 cgagagcatg gctgggaagt ctgcagcttt gcacggtctc tgccatgatg ctacaccctt
     2941 catcttctcc gaggagaact ctgccttaga gtactttggt gagatgttaa aggctgctgg
     3001 ctacaacttc tacggcaccg agagactgta tagtggcatc agtgggatgg agctggaggc
     3061 tgacatcttc attggtgtgg tttattacca gcgcttacga cacatggtgt cagacaaatt
     3121 tcaagtcaga acaactggag ccagggacaa agtcaccaac cagcccctcg ggggacgaaa
     3181 tgttcagggt ggaatccgct ttggggagat ggagcgggat gctctgttgg cacacggtac
     3241 gtctttcctt cttcatgacc ggcttttcaa ctgctctgac cgttctgtcg ctcacatgtg
     3301 tgtagagtgt ggcagtttgc tttctccgct acttgagaag cctcctccgt cttggtctgc
     3361 gatgcgcaac agaaagtaca actgcactgt ctgtggccgc agtgacacca tcgacaccgt
     3421 ctctgtgccg tatgtcttcc ggtactttgt agctgagctg gcagccatga acatcaaagt
     3481 gaaactggat gtcatctgac ttgatccccg ccatctgagc taggagataa gaacaggagg
     3541 tgtcctgtat aacacaagtg agggtattat gggctcactc tgggtctata caagaatttc
     3601 agtactgaag ccaagaacag gcaggtgaaa attcaagact tctgagtctc tgtaacctac
     3661 tgaagttggt tttcatatct ttttcaagac agggtttctc tgtgtagccc tggctgtcct
     3721 ggaactcact ttgtagacca ggctggtctc aaactcagaa atccacctgc ctctgcctcc
     3781 tgagtgctgg gattaaaggc gtgcgccacc acgcccagcc tcatatcttt ttttaaaaaa
     3841 gttatttttg tgcctttaaa aaatgactta attgataata gatcatgcag ggcagggcgc
     3901 cttctttctg ggcccaggtt cactgctccc tgctttcagt agcagagggt gtctgttatc
     3961 cagagcaggc cagtacaata aacagaaaat ggaagccaaa aaaaaaaaaa aaaaaaa
//
