LOCUS       NM_029977               8569 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus polymerase (DNA directed), theta (Polq), transcript
            variant 1, mRNA.
ACCESSION   NM_029977 XM_148122
VERSION     NM_029977.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Wang,N., Zhang,S., Langfelder,P., Ramanathan,L., Gao,F.,
            Plascencia,M., Vaca,R., Gu,X., Deng,L., Dionisio,L.E., Vu,H.,
            Maciejewski,E., Ernst,J., Prasad,B.C., Vogt,T.F., Horvath,S.,
            Aaronson,J.S., Rosinski,J. and Yang,X.W.
  TITLE     Distinct mismatch-repair complex genes set neuronal CAG-repeat
            expansion rate to drive selective pathogenesis in HD mice
  JOURNAL   Cell (2025) In press
   PUBMED   39938516
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Shah,P., Hill,R., Dion,C., Clark,S.J., Abakir,A., Willems,J.,
            Arends,M.J., Garaycoechea,J.I., Leitch,H.G., Reik,W. and
            Crossan,G.P.
  TITLE     Primordial germ cell DNA demethylation and development require DNA
            translesion synthesis
  JOURNAL   Nat Commun 15 (1), 3734 (2024)
   PUBMED   38702312
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8569)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 8569)
  AUTHORS   Vekariya,U., Toma,M., Nieborowska-Skorska,M., Le,B.V., Caron,M.C.,
            Kukuyan,A.M., Sullivan-Reed,K., Podszywalow-Bartnicka,P.,
            Chitrala,K.N., Atkins,J., Drzewiecka,M., Feng,W., Chan,J.,
            Chatla,S., Golovine,K., Jelinek,J., Sliwinski,T., Ghosh,J.,
            Matlawska-Wasowska,K., Chandramouly,G., Nejati,R., Wasik,M.,
            Sykes,S.M., Piwocka,K., Hadzijusufovic,E., Valent,P.,
            Pomerantz,R.T., Morton,G., Childers,W., Zhao,H., Paietta,E.M.,
            Levine,R.L., Tallman,M.S., Fernandez,H.F., Litzow,M.R., Gupta,G.P.,
            Masson,J.Y. and Skorski,T.
  TITLE     DNA polymerase theta; protects leukemia cells from metabolically
            induced DNA damage
  JOURNAL   Blood 141 (19), 2372-2389 (2023)
   PUBMED   36580665
REFERENCE   5  (bases 1 to 8569)
  AUTHORS   Smolinska,A., Singer,K., Golchert,J., Smyczynska,U., Fendler,W.,
            Sendler,M., van den Brandt,J., Singer,S., Homuth,G., Lerch,M.M. and
            Moskwa,P.
  TITLE     DNA Polymerase Theta Plays a Critical Role in Pancreatic Cancer
            Development and Metastasis
  JOURNAL   Cancers (Basel) 14 (17), 4077 (2022)
   PUBMED   36077614
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8569)
  AUTHORS   Masuda,K., Ouchida,R., Takeuchi,A., Saito,T., Koseki,H.,
            Kawamura,K., Tagawa,M., Tokuhisa,T., Azuma,T. and O-Wang,J.
  TITLE     DNA polymerase theta contributes to the generation of C/G mutations
            during somatic hypermutation of Ig genes
  JOURNAL   Proc Natl Acad Sci U S A 102 (39), 13986-13991 (2005)
   PUBMED   16172387
  REMARK    GeneRIF: Polq-inactive mice exhibited a reduced level of serum IgM
            and IgG1.
REFERENCE   7  (bases 1 to 8569)
  AUTHORS   Shima,N., Munroe,R.J. and Schimenti,J.C.
  TITLE     The mouse genomic instability mutation chaos1 is an allele of Polq
            that exhibits genetic interaction with Atm
  JOURNAL   Mol Cell Biol 24 (23), 10381-10389 (2004)
   PUBMED   15542845
  REMARK    GeneRIF: Polq has a role in maintaining genomic integrity, which is
            probably distinctive from the major homologous recombination
            pathway regulated by ATM
REFERENCE   8  (bases 1 to 8569)
  AUTHORS   Kawamura,K., Bahar,R., Seimiya,M., Chiyo,M., Wada,A., Okada,S.,
            Hatano,M., Tokuhisa,T., Kimura,H., Watanabe,S., Honda,I.,
            Sakiyama,S., Tagawa,M. and O-Wang,J.
  TITLE     DNA polymerase theta is preferentially expressed in lymphoid
            tissues and upregulated in human cancers
  JOURNAL   Int J Cancer 109 (1), 9-16 (2004)
   PUBMED   14735462
  REMARK    GeneRIF: DNA Pol theta has a specialized function in lymphocytes
            and in tumor progression
REFERENCE   9  (bases 1 to 8569)
  AUTHORS   Marini,F., Kim,N., Schuffert,A. and Wood,R.D.
  TITLE     POLN, a nuclear PolA family DNA polymerase homologous to the DNA
            cross-link sensitivity protein Mus308
  JOURNAL   J Biol Chem 278 (34), 32014-32019 (2003)
   PUBMED   12794064
REFERENCE   10 (bases 1 to 8569)
  AUTHORS   Shima,N., Hartford,S.A., Duffy,T., Wilson,L.A., Schimenti,K.J. and
            Schimenti,J.C.
  TITLE     Phenotype-based identification of mouse chromosome instability
            mutants
  JOURNAL   Genetics 163 (3), 1031-1040 (2003)
   PUBMED   12663541
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY074936.1, AK139505.1 and CO041834.1.
            
            On Apr 15, 2009 this sequence version replaced NM_029977.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY074936.1, BC138360.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7839              AY074936.1         1-7839
            7840-8544           AK139505.1         993-1697
            8545-8569           CO041834.1         1-25                c
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 26.32 cM"
     gene            1..8569
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /note="polymerase (DNA directed), theta"
                     /db_xref="GeneID:77782"
                     /db_xref="MGI:MGI:2155399"
     exon            1..274
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..7749
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /EC_number="2.7.7.7"
                     /EC_number="2.7.7.49"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase eta; chromosome aberrations occurring
                     spontaneously protein 1; DNA polymerase theta"
                     /codon_start=1
                     /product="DNA polymerase theta isoform 1"
                     /protein_id="NP_084253.1"
                     /db_xref="CCDS:CCDS28158.1"
                     /db_xref="GeneID:77782"
                     /db_xref="MGI:MGI:2155399"
                     /translation="MSLPRRSRKRRRSSSGSDTFSGDGDSFVSPQLRCGPVLSPPPGL
                     GRGRRLTGTGTNKRRVSDDQIDQLLLANWGLPKAVLEKYHSFGVRKMFEWQAECLLLG
                     HVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQSLF
                     QEVGLKVDGYMGSTSPTGQFSSLDIAVCTIERANGLVNRLIEENKMDLLGMVVVDELH
                     MLGDSHRGYLLELLLTKICYVTRKSASHQAESASTLSNAVQIVGMSATLPNLQLVASW
                     LNAELYHTDFRPVPLLESIKIGNSIYDSSMKLVREFQPLLQVKGDEDHIVSLCYETIQ
                     DNHSVLIFCPSKKWCEKVADIIAREFYNLHHQPEGLVKSSEFPPVILDQKSLLEVMDQ
                     LKRSPSGLDSVLKNTVPWGVAFHHAGLTFEERDIIEGAFRQGFIRVLAATSTLSSGVN
                     LPARRVIIRTPIFSGQPLDILTYKQMVGRAGRKGVDTMGESILVCKNSEKSKGIALLQ
                     GSLEPVHSCLQRQGEVTASMIRAILEIIVGGVASTSQDMQTYAACTFLAAAIQEGKQG
                     MQRNQDDAQLGAIDACVTWLLENEFIQVAEPGDGTGGKVYHPTHLGSATLSSSLSPTD
                     TLDIFADLQRAMKGFVLENDLHIVYLVTPVFEDWISIDWYRFFCLWEKLPTSMKRVAE
                     LVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEIPLKDINQKYGC
                     NRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLIRVSL
                     LNAQRARFLYASGFLTVADLARADSAEVEVALKNSLPFKSARKAVDEEEEAAEERRSM
                     RTIWVTGKGLSAREAAALIVEEAKMILQQDLIEMGVRWDPKSPLSSSTHSRTSTSEVK
                     EHTFKSQTKSSHKRLASMGRNSIRASGSNDKPSPDAERGIDDCSEHADSLCKFQGNFE
                     PQTPSICTARKRTSLGINKEMLRKSLKEGKPSTKEVLQTFSSEKTRKTALSFSSEQVN
                     NTLPSGRDRKYQKKSWGSSPVRDSGMHRGDLQGQTMCTSALCEDSQKSLEEQNAEFRS
                     PGLFAKHLPSCAKEKCKKPSLPLQRQQACSRRSTESCAAVGHPAAGSSPAAARDRRGL
                     AARETEKGNEALTENGGESQLQDTYPVSQYLEYHSEKHTNTCTRQKTLTEGQAGSSYV
                     ARDSNDAAPIKCERMKLNSKDRDSNPCRQALGSYTGRTEALQSTAKLGQAGGQCENLL
                     NSSGVQGKTGAHATNRTEHSHASNPAFCDFGDSLDLDTQSEEIIEQMATENTMQGAKA
                     VVIMEEGSAMQNKCHSTPGDQHVPGAANTDHVDSKKVESVKANTEKNINRGAPVSLIF
                     HTQGENGACFKGNEHSVTDSQLNSFLQGFETQEIVKPIIPLAPQMRTPTGVEEESLPE
                     TSLNMSDSILFDSFGEDGFGQGQSPDIKANQPLLSEMTPNHFSNPPHPQEDPVMTPTV
                     SEPQGTQQQGVCLSGESIIFSDIDSAQVIEALDNMAAFHVQENCNSVALKTLEPSDSA
                     VLGNECPQGKLVRGDQNEGSPKPKLTETNQDNSFTWSGASFNLSPELQRILDKVSSPR
                     ENEKPKMIHVNLSSFEGNSKESHEREEINSDLGTVQRTSVFPSNEVKNRTEGLESKAR
                     HGGASSPLPRKESAAADDNGLIPPTPVPASASKVAFPEILGTSVKRQKASSALQPGES
                     CLFGSPSDNQNQDLSQELRDSLKDYDGSVADTSFFLQSQDGLLLTQASCSSESLAIID
                     VASDQILFQTFVKEWQCQKRFSISLACEKMTSSMSSKTATIGGKLKQVSLPQEATVED
                     AGFPVRGCDGAVVVGLAVCWGAKDAYYLSLQKEQKQSEISPSLAPPPLDATLTVKERM
                     ECLQSCLQKKSDRERSVVTYDFIQTYKVLLLSCGISLEPSYEDPKVACWLLDPDSKEP
                     TLHSIVTSFLPHELALLEGMETGPGIQSLGLNVNTEHSGRYRASVESVLIFNSMNQLN
                     SLLQKENLHDIFCKVEMPSQYCLALLELNGIGFSTAECESQKHVMQAKLDAIETQAYQ
                     LAGHSFSFTSADDIAQVLFLELKLPPNGEMKTQGSKKTLGSTRRGNESGRRMRLGRQF
                     STSKDILNKLKGLHPLPGLILEWRRISNAITKVVFPLQREKHLNPLLRMERIYPVSQS
                     HTATGRITFTEPNIQNVPRDFEIKMPTLVRESPPSQAPKGRFPMAIGQDKKVYGLHPG
                     HRTQMEEKASDRGVPFSVSMRHAFVPFPGGLILAADYSQLELRILAHLSRDCRLIQVL
                     NTGADVFRSIAAEWKMIEPDAVGDDLRQHAKQICYGIIYGMGAKSLGEQMGIKENDAA
                     SYIDSFKSRYKGINHFMRDTVKNCRKNGFVETILGRRRYLPGIKDDNPYHKAHAERQA
                     INTTVQGSAADIVKIATVNIQKQLETFRSTFKSHGHRESMLQNDRTGLLPKRKLKGMF
                     CPMRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMECAIKLSVKLKVKVKIGASWGE
                     LKDFDV"
     exon            275..454
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            455..585
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            586..742
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            743..851
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1071
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1219
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1366
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1579
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1716
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1921
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2064
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2258
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2383
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2627
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..5608
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5609..5752
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5753..5949
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5950..6190
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6191..6384
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6385..6522
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6523..6697
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6698..6821
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6822..6943
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            6944..7128
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            7129..7240
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            7241..7365
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            7366..7519
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            7520..7635
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            7636..8552
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8533..8538
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /note="hexamer: AATAAA"
     polyA_site      8552
                     /gene="Polq"
                     /gene_synonym="A430110D14Rik"
                     /note="major polyA site"
ORIGIN      
        1 gaagatggag gcgggaagat ggaggcggta gctgtcacta ggttggggtt ctcccgcgcg
       61 ggaggcctat gggactgtgt cgtgtcctga acagtcgaga ggagcttgtt tgcaatgagt
      121 cttccgcgcc ggagtaggaa acggcggcgt tcatcgtccg gctccgacac gttctcggga
      181 gatggtgata gctttgttag ccctcagctc cggtgtggac cggtgctgag tccacctccg
      241 gggctgggac gcggccggag gctcacaggg acaggaacaa acaagcgaag agtttctgat
      301 gaccagatag accagctgct gttggcaaat tggggacttc ctaaagcagt tctggagaaa
      361 taccatagtt ttggtgtaag aaagatgttc gaatggcagg cagaatgcct tttgcttggt
      421 cacgtcttgg aaggaaagaa tctagtttat tcagctccta caagtgctgg gaagactctt
      481 gttgcagagt tacttattct gaagcgggtt ttggaaacac ggaagaaagc gttgtttatt
      541 ttgccctttg tgtctgtggc taaagagaag aaatgctacc tccagagtct gtttcaggaa
      601 gtaggactaa aggtagatgg ctatatgggc agcacctcac caacgggaca gttctcctcc
      661 ttggacattg ctgtttgcac tattgagaga gccaatgggc tggtcaatcg cctcattgag
      721 gaaaataaga tggatctgtt aggaatggtg gtcgtggatg agctgcacat gctgggggac
      781 tctcaccgag ggtatctgct ggagctgttg ctgaccaaga tttgctacgt cactcgtaaa
      841 tcagcatcgc accaggcaga gtcagccagc actctgtcta atgctgtgca gattgttggc
      901 atgagtgcta ctcttcctaa tttgcagctt gtggcttcct ggctgaatgc tgaactttac
      961 cataccgact ttcgccctgt accacttttg gaatcaataa aaataggaaa ttccatatat
     1021 gattcttcaa tgaagcttgt gagagaattt caacccctgc tccaagtgaa gggagatgaa
     1081 gaccacattg ttagtttatg ctatgagacc attcaggata accattcagt attaatcttt
     1141 tgtccatcaa aaaaatggtg tgagaaggta gcagatatca ttgcccgaga gttttacaat
     1201 ctgcaccatc aacctgaggg gttggtaaaa tcatctgaat ttccaccagt gattctagac
     1261 caaaagagcc ttctggaagt gatggatcag ttaaaacgtt caccttcagg actagactct
     1321 gtgttgaaga atactgtgcc atggggagta gcgtttcatc atgcaggtct tacttttgag
     1381 gagagggata tcatcgaagg agccttccgt caaggtttca ttcgggtctt ggcagcaacg
     1441 tctactcttt cttctggagt gaatcttcct gctcgtcggg tgattattcg cactccaatt
     1501 ttcagtggtc agcctttaga tatcctcact tataagcaga tggttgggcg tgctggcagg
     1561 aaaggagtag acacaatggg tgaaagtatc ctagtttgta agaactctga gaaatccaag
     1621 ggcattgctc tgcttcaagg atctctggag cctgtgcaca gctgtctgca gagacaggga
     1681 gaagtcactg ctagcatgat acgagctatt ctggagataa tcgttggtgg ggtggccagt
     1741 acgtcacaag atatgcagac ttatgccgcc tgcaccttct tggctgctgc tatacaggaa
     1801 gggaagcaag ggatgcagag aaatcaagat gatgctcagc ttggagcgat tgatgcctgt
     1861 gtgacgtggc tgttagaaaa tgagttcatc caggtggcag agcccgggga cggcacagga
     1921 ggaaaggtgt atcacccaac acatcttgga tcagctactc tttcttcttc actgtctcca
     1981 actgataccc ttgatatttt tgctgacctc caaagagcaa tgaaaggctt tgttttagag
     2041 aatgaccttc atattgtcta cctggttaca cctgtgtttg aagattggat tagtattgat
     2101 tggtaccgat ttttctgttt atgggaaaaa ttaccaactt ctatgaaaag ggtggcagag
     2161 ctggttggag tggaggaagg gttcttggct cgatgtgtga aaggaaaagt agtagctaga
     2221 actgagagac agcatcggca gatggccatc cataaaaggt ttttcacaag tcttgtgtta
     2281 ctggatttaa tcagtgaaat tcccttaaag gacattaatc agaagtatgg atgtaatcgt
     2341 ggacagattc agtctttgca acagtcagct gctgtctatg caggaatgat tacggtgttt
     2401 tccaaccgcc tgggctggca caacatggaa ctactgcttt cccagtttca gaagcgtctt
     2461 acgtttggca tccagagaga gctgtgtgac ctcatccggg tgtccttact caacgcgcag
     2521 agagccaggt tcctgtatgc ctctggcttt ctcaccgtgg cagatcttgc cagagctgat
     2581 agtgcggagg tagaggtggc tctgaagaac tctttgcctt tcaaaagtgc ccggaaggca
     2641 gtggatgaag aagaggaagc agctgaagaa cggcggagta tgagaactat ttgggtgact
     2701 ggcaagggct tatctgcaag ggaagcagca gccttaatag tggaagaagc taaaatgatt
     2761 ctacagcagg acttaattga aatgggagta cggtgggatc cgaagtcacc cttgagttct
     2821 agcacacact cacggaccag cacgtcagaa gtaaaggaac acacgttcaa atcccagact
     2881 aagagttctc ataaaaggtt agcatcaatg ggcagaaata gcattagagc aagtgggtct
     2941 aatgacaagc cgtccccaga tgcagagcga ggcatagatg actgcagcga gcatgcagac
     3001 tctctctgta agttccaggg caatttcgag ccccagacac cctccatttg tacagcaaga
     3061 aaacggactt ctttaggtat aaacaaagaa atgcttcgaa agtctctgaa agaaggaaag
     3121 ccaagcacta aggaagttct tcaaactttc tcatcggaga aaacaagaaa aactgctttg
     3181 agttttagtt cagagcaagt gaacaacact ttaccatctg ggagagacag aaagtatcag
     3241 aaaaaatctt ggggcagtag tcctgtgcgt gactctggga tgcatagagg tgatttacag
     3301 ggacagacta tgtgtacatc tgctctatgt gaagactcac agaagtcctt agaggagcag
     3361 aatgcagaat ttagaagtcc agggctattt gcaaaacatc taccttcttg tgccaaagaa
     3421 aaatgcaaga agccctcact ccctttacaa aggcagcagg cttgctcaag gagaagcaca
     3481 gagagctgtg ctgctgtggg gcatcccgct gcagggtcgt cccctgctgc tgctagagac
     3541 agaagaggac tggctgctcg ggagacagaa aagggaaatg aggcgctcac ggagaatggt
     3601 ggagaaagcc agctccaaga cacctaccca gtcagccagt acctggaata ccacagtgag
     3661 aaacacacaa acacttgcac cagacagaag acattaacag agggacaagc tggtagtagt
     3721 tatgtggcca gagactcaaa tgatgctgca cccatcaaat gtgaacgtat gaagcttaat
     3781 agcaaagacc gcgactcaaa tccttgtcgt caggcattgg gaagttacac aggcaggact
     3841 gaggcacttc agtcaactgc aaaacttggc caagcaggag gccagtgtga aaatcttcta
     3901 aattcttctg gagtacaagg aaaaacaggt gctcatgcaa caaatagaac tgaacatagt
     3961 catgcttcta acccagcctt ctgtgacttt ggagatagct tagacttgga tactcagtca
     4021 gaggaaataa ttgagcagat ggcaactgaa aataccatgc aaggagcaaa agcagtagtg
     4081 ataatggaag agggttccgc catgcagaac aagtgtcaca gcactccagg tgaccagcat
     4141 gtcccaggag cagccaacac agaccatgtg gacagtaaga aggttgagtc tgtgaaagca
     4201 aacaccgaaa agaacatcaa tagaggcgct ccagtcagcc tcatcttcca tactcaaggg
     4261 gaaaatggcg cttgctttaa agggaatgag cattctgtta ctgattccca attaaacagt
     4321 tttcttcaag ggtttgaaac acaagaaata gtgaagccaa tcataccgct ggctcctcaa
     4381 atgagaactc ctactggtgt ggaggaagaa agtctgcctg aaaccagttt gaatatgagt
     4441 gatagtatac tatttgacag ttttggagaa gatggcttcg gacaaggaca gtcacctgat
     4501 ataaaggcaa atcagccact cctttctgaa atgacaccaa accatttcag caatcctcca
     4561 cacccacaag aagacccagt tatgacacca actgtgagtg agcctcaggg tactcagcag
     4621 caaggagttt gtctcagtgg tgaatctatt atattttcag acatagactc tgctcaggtc
     4681 attgaagcct tagacaatat ggctgcattt catgtgcaag agaactgtaa ctcagtagcc
     4741 cttaaaacat tagaacccag tgattctgca gtgctgggta atgagtgtcc ccaaggaaaa
     4801 ctggtcagag gagatcaaaa tgaagggtcc ccaaagccca aactcacaga gacaaatcaa
     4861 gataattcat tcacttggtc aggagcatca tttaacttaa gtccagaact gcaaaggatt
     4921 ttagataaag tatctagtcc tcgagaaaat gaaaagccca agatgataca tgtaaacttg
     4981 tcttcctttg aaggaaacag caaagagtca catgaaagag aggaaataaa ttcagatttg
     5041 gggacagttc agagaacttc agttttcccc agtaatgaag ttaaaaacag gactgaaggg
     5101 ctagagagca aagccaggca tggtggagcc tcatctccct tgcctcgtaa agaaagtgct
     5161 gcagctgatg acaatggcct cattcctcct acacccgttc ctgcttctgc ttctaaggta
     5221 gcctttccag agattctcgg aacatctgta aaacgtcaga aagccagcag tgctttacag
     5281 ccaggggaaa gttgtttatt tggctcacct tcagacaatc aaaaccagga tttgagtcaa
     5341 gagcttagag acagcctcaa ggactatgat ggttctgttg cagacacaag tttttttctg
     5401 cagtctcagg atggactgct gttaactcaa gcctcatgca gctcagagag tctggccata
     5461 attgacgtag cgagtgatca gattctcttt cagacatttg tgaaggagtg gcaatgccaa
     5521 aagcgctttt ccatctctct ggcttgtgaa aagatgacaa gttcaatgtc ttccaaaact
     5581 gctaccatag gtggaaagtt gaagcaagtt agcttgccac aggaagccac tgttgaagat
     5641 gctgggttcc ctgtgcgtgg ctgtgatggt gctgtggtag ttggactagc agtgtgctgg
     5701 ggtgcaaagg atgcctatta tttgtcactg cagaaggaac aaaagcagtc tgaaataagt
     5761 cccagtttgg ccccaccacc tctggacgca acactaactg tgaaggagag gatggagtgc
     5821 cttcagtcct gcttgcaaaa gaagtctgat cgagagcggt ctgttgtcac ctatgatttc
     5881 atccagacct ataaagtcct cctcctgtca tgtggcatct ccctggagcc cagttatgaa
     5941 gaccccaagg tggcatgctg gcttctcgac ccagattcta aggagccaac tcttcatagc
     6001 atagtgacca gttttctccc ccatgagcta gcactcctag aaggaatgga gacaggccca
     6061 ggaattcaga gcctggggct aaatgtgaac actgaacatt ctgggaggta cagagcatct
     6121 gtggagtctg ttctcatctt caactccatg aatcaactca actctttgtt gcagaaggaa
     6181 aaccttcatg atattttctg caaagtggag atgccctctc agtactgcct ggccttgcta
     6241 gaactaaatg gaattggctt tagtactgcg gagtgtgaaa gccagaagca tgttatgcaa
     6301 gccaagctgg acgccattga gacccaagcc tatcagctgg ctggccacag tttttccttt
     6361 acaagtgcag atgacatcgc acaggtttta tttttggaac taaagttacc accgaatgga
     6421 gagatgaaaa cgcaaggcag caaaaagact ctgggttcca ccagaagagg gaatgagagt
     6481 ggccgcagaa tgaggctggg aagacagttc agcactagta aggatatttt aaataaatta
     6541 aagggcttgc atcctttacc aggcctgata ctggaatgga gaagaatcag taatgctatt
     6601 accaaggtgg tcttccctct gcagcgggaa aagcacctga acccccttct cagaatggag
     6661 aggatctacc cggtgtcaca gtcacacacg gctacaggac ggataacctt tacagaaccc
     6721 aatatccaga atgtgccaag agattttgag attaaaatgc caacactagt aagggagagc
     6781 ccaccttctc aagctccaaa aggccggttc ccaatggcaa taggacaaga taagaaggtt
     6841 tatggcctgc accccgggca cagaacacag atggaagaga aggcctcaga tagaggggtg
     6901 ccattttcag tgagcatgcg ccatgccttt gtgcctttcc caggtgggct gatcctggct
     6961 gccgactact ctcagcttga actgaggatc ctggctcact tgtctcgtga ctgtcgtctc
     7021 attcaagtct taaatacggg agcggatgtg ttcaggagca ttgctgccga gtggaagatg
     7081 attgagcctg atgctgttgg tgacgatctg aggcagcacg caaagcagat ttgctatgga
     7141 attatatatg gaatgggagc taaatcttta ggagagcaga tgggcattaa agaaaatgat
     7201 gctgcttcct acattgactc tttcaagtcc agatataaag ggattaatca tttcatgagg
     7261 gacacagtga agaactgtag aaaaaatgga tttgttgaga caattttggg aagacgtaga
     7321 tatttgccag gaatcaaaga cgacaatcct tatcataaag cacacgctga acgtcaggcc
     7381 atcaacacaa cagtccaggg atcagctgct gatatagtca aaatagccac agttaatatt
     7441 cagaagcaat tagagacctt ccgttcaacc ttcaaatccc acggtcatcg ggaaagtatg
     7501 ctccagaatg accgaacagg attgttgccc aagaggaaac ttaaagggat gttttgtccc
     7561 atgagaggag gattcttcat ccttcaactt cacgacgagc ttttatatga agtggcagaa
     7621 gaggatgttg ttcaggtggc tcagattgtc aagaatgaaa tggaatgtgc tataaaactt
     7681 tctgtgaagc tgaaagtgaa agtgaaaata ggtgccagct ggggagagct gaaggacttt
     7741 gatgtgtgat ggtacagtta accagatccc cagagcagcc tggtagacag tcacttagaa
     7801 tgcatagagt ctgccctttg acctgctctt aaacaagcag gtctccagtg ggtttctaat
     7861 gtatacctca gtccagcccc tgtggtgtca caaggctttg aggggagatt tattagttat
     7921 cctcactctc aaagagtatc tatgaatttg cctttcataa tttattggag tgttaaacag
     7981 tctcagaatg tatgcttgaa aaatgcactt agcactttgg gttctcaact gtgtagaatg
     8041 tgaaggagtg gagctgctgc ctcagggcca tttttattcc tgtcaaggac tccagcactc
     8101 ataatgtaat cccccaagag ccacgggcag tgctcagctc cagtcattag agttatgcag
     8161 tttgtgtgat ctttttctac agtaaaaact tctgaagtag aattttaaaa tctcaaattg
     8221 aaaatctata agacagtaag ctgtgtgaaa ggcatctaga atgagttttc atgaactgtt
     8281 gccgcttgtt cttctcatcg gcggtccaac aaggaaggat ttagagcact cttaaaatga
     8341 gctgttcccc ataagaagtg tctgtcagct tagacattga ttgcacttga acctaacact
     8401 gccaggccag gaaaacgccc ctttgctagc tagcccctta gtgcctgtat ttgtggtatg
     8461 atatgaagag aattctcagg cttaaaacat tggatttctt tactcataac taatatatct
     8521 ttttgctgtt caaataaatt tatgggaatt taaaaaaaaa aaaaaaaaa
//
