LOCUS       NM_181857               2994 bp    mRNA    linear   ROD 05-AUG-2023
DEFINITION  Mus musculus DNA polymerase N (Poln), transcript variant 1, mRNA.
ACCESSION   NM_181857 XM_197865
VERSION     NM_181857.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Takata KI, Reh S, Yousefzadeh MJ, Zelazowski MJ, Bhetawal S, Trono
            D, Lowery MG, Sandoval M, Takata Y, Lu Y, Lin K, Shen J, Kusewitt
            DF, McBride KM, Cole F and Wood RD.
  TITLE     Analysis of DNA polymerase nu function in meiotic recombination,
            immunoglobulin class-switching, and DNA damage tolerance
  JOURNAL   PLoS Genet 13 (6), e1006818 (2017)
   PUBMED   28570559
  REMARK    GeneRIF: Inactivation of Poln in mouse embryonic fibroblasts did
            not alter cellular sensitivity to mitomycin C, cisplatin, or
            aldehydes.  Pol nu-defective mice had no alteration in DNA
            end-joining during immunoglobulin class-switching.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK,
            Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L,
            Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre
            JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A,
            Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ,
            Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall
            S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA,
            Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR,
            Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana
            S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, MacArthur DG,
            Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC,
            Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL,
            de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown
            SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bucan M and Murray
            SA.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   3  (bases 1 to 2994)
  AUTHORS   Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H,
            Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T,
            Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P,
            Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ,
            Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD,
            Flicek P, Skarnes WC, Mallon AM and Parkinson H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2994)
  AUTHORS   van der Laan R, Uringa EJ, Wassenaar E, Hoogerbrugge JW, Sleddens
            E, Odijk H, Roest HP, de Boer P, Hoeijmakers JH, Grootegoed JA and
            Baarends WM.
  TITLE     Ubiquitin ligase Rad18Sc localizes to the XY body and to other
            chromosomal regions that are unpaired and transcriptionally
            silenced during male meiotic prophase
  JOURNAL   J Cell Sci 117 (Pt 21), 5023-5033 (2004)
   PUBMED   15383616
REFERENCE   5  (bases 1 to 2994)
  AUTHORS   Marini F, Kim N, Schuffert A and Wood RD.
  TITLE     POLN, a nuclear PolA family DNA polymerase homologous to the DNA
            cross-link sensitivity protein Mus308
  JOURNAL   J Biol Chem 278 (34), 32014-32019 (2003)
   PUBMED   12794064
  REMARK    GeneRIF: identification as homolog to Mus308
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ083086.1, AK133063.1, AC107709.12 and BY585631.1.
            
            [WARNING] On Apr 1, 2024 this sequence was replaced by NM_181857.5.
            
            On Jan 31, 2014 this sequence version replaced NM_181857.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY135562.3, BC141101.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                CJ083086.1         2-82
            82-137              AK133063.1         2-57
            138-2211            AK133063.1         107-2180
            2212-2343           AC107709.12        150963-151094       c
            2344-2983           AK133063.1         2181-2820
            2984-2994           BY585631.1         382-392
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.83 cM"
     gene            1..2994
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /note="DNA polymerase N"
                     /db_xref="GeneID:272158"
                     /db_xref="MGI:MGI:2675617"
     exon            1..137
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            138..282
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..2744
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed DNA polymerase nu"
                     /codon_start=1
                     /product="DNA polymerase nu isoform 1"
                     /protein_id="NP_862905.1"
                     /db_xref="CCDS:CCDS39067.1"
                     /db_xref="GeneID:272158"
                     /db_xref="MGI:MGI:2675617"
                     /translation="MKMENYEACVGFDVCEIPLSAVAQKIMSAMRSGDFMDSRNEGES
                     TNTSKVAKKSSVHYSVLAEHEETQSLGTKNPESLITQTPRGSIELCPQPSITKLTCQL
                     SAGQVQNSISSLGLSSYLIPQCDQEASVLPNMEHKRQHFLKENIGKEDKDNSSLKRKY
                     ITCSKSSEKASKHTALEKDTDGTESWPNSRDTRALGERLCDVRYLGDLAKAQLMDALK
                     QAAALVVTLMYKDGSTQLSAKEALTCTVKGIVVLLKSHVGNSTLTLPAHGGALEKDFI
                     SEDHCVYIHTEHSPFWDPKQEAHSLFVRNILFWTLRCKCPVVCFNAKDFVRTVLQLYG
                     EDGSWKHVADFVGLDPRVAAWLIDPSDTAPSFEDLVAKHLEKSITVKPSSTFREASRN
                     TLSQNVFMNLKILYDLTMDLCSKLKAYGLWQLFCTLELPLIPILAVMENHKIPVDKEE
                     MERTSALLGARLKELEQEAHFVAGEQFLIMSNNQLREILFGKLKLHLLSQRKHLPRTG
                     LQNQLSTSEAMLNSLQDLHPLPKLILEYRQVHKIKSTFIDGLLAYMKKGSISSTWNQT
                     GTVTGRLSAKHPNIQGISKHPIKISKPWNFKGKEEETVTISPRTLFVSSEGHTFLAAD
                     FSQIELRILAHLSGDPELLKLFQESERDDVFSTLTSQWKDIPIERVTHMDREQTKKVV
                     YSVVYGAGKERLAACLGVTVLEATHFLERFLQKYKKIKDFAQTVIGQCHSAGYVTSIL
                     GRRRPLPRICAQDQQLRAQAERQAVNFVVQGSAADLCKLAMIRISTAVATSPTLTARL
                     VAQIHDELLFEVEDTQVPEFAALVRRIMESLQQVQTLELQLQVPLKVNLSVGRSWGHL
                     TPLQEILGSA"
     exon            283..362
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            363..863
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            864..1054
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1167
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1325
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1394
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1455
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1520
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1604
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1700
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1757
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1811
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1877
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1935
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2013
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2128
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2211
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2343
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2454
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2533
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2601
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2663
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
     exon            2664..2994
                     /gene="Poln"
                     /gene_synonym="POL4P"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttctccggga tacctcgccg cctgcgctga ctttacggat gttgtaatct gcgcgtgcgc
       61 gtcctggcac tcccgtgagg cactgcggcg cgcgcggttg gaggctggca gagagcagtg
      121 actgcggctt tcctgaggac tttatgaaaa tggaaaatta tgaggcatgt gtaggttttg
      181 atgtctgtga gatacccctc tctgctgttg ctcagaagat tatgtctgct atgcgttcag
      241 gtgatttcat ggattctagg aatgagggag agagtacaaa cacttcaaaa gtggcaaaga
      301 aatccagtgt tcattactca gtattagctg aacatgagga gactcaatca ctaggaacaa
      361 agaaccccga gtcattaata acccagacac caagaggatc cattgagctc tgtcctcagc
      421 cctccattac taagctcaca tgtcagctct ctgctggtca agtacagaac agcatcagct
      481 cattgggtct ttccagttat ttaattccac agtgtgatca ggaagcttca gttttaccga
      541 acatggagca taagagacag catttcctaa aggaaaatat aggcaaagaa gataaggaca
      601 actcgagtct taaaagaaaa tatattacat gtagtaaatc ctcagaaaaa gcaagtaaac
      661 acacagctct ggaaaaagat actgatggga ctgaatcctg gccaaactcc agggacacaa
      721 gagctttggg ggaaaggctg tgtgatgtta gatatttggg tgatttggcc aaagcccagc
      781 tgatggatgc tctcaagcag gcagcagccc tggtggtgac attgatgtac aaggatggtt
      841 ccacacagct gagcgccaag gaggctttaa cttgtactgt taaaggaatt gtagtgttac
      901 taaaaagcca tgtaggcaac agcactctga ctctcccagc ccatggtggt gctctggaaa
      961 aggacttcat atccgaagat cactgtgtct acatacatac ggagcattcc cctttctggg
     1021 acccaaagca agaagcacat agtctatttg tcaggaacat actgttttgg acactgaggt
     1081 gtaaatgtcc cgttgtttgt tttaatgcca aggactttgt gagaacagtg ctgcagctct
     1141 atggtgaaga tggcagttgg aagcatgttg ctgattttgt aggactagat cccagagttg
     1201 ctgcatggct catagacccc agtgacactg caccttcatt tgaagattta gtagcaaaac
     1261 acctagaaaa gtccatcaca gtcaaaccaa gcagcacatt cagagaagcc tcaagaaata
     1321 ctttgagtca gaatgtattt atgaatctga agatactcta cgaccttaca atggatcttt
     1381 gttctaagtt gaaggcttat ggcttatggc agctattttg cactttggag cttccattga
     1441 taccaatttt ggcagtgatg gaaaaccata agattccagt tgacaaagaa gaaatggaga
     1501 ggacatcggc actcctcggg gctcgcctca aggaattgga gcaagaagcc cattttgttg
     1561 caggagaaca gtttcttata atgagtaata atcaacttcg tgagatcctc tttggcaagt
     1621 tgaagctgca cttgttgagt cagaggaaac atcttcccag aactgggctg cagaaccagc
     1681 tgtccacatc agaagccatg ttaaattctc tacaagacct tcacccatta cccaagttaa
     1741 ttttggaata tagacaggtt cacaagatca agtcaacttt tatagatgga ttactagctt
     1801 atatgaaaaa gggctccatc tcttctacat ggaatcagac tggaactgtg acagggagac
     1861 tttcagccaa gcatccaaat atccagggta tctccaagca cccaattaag atttctaagc
     1921 cttggaattt taaaggtaaa gaagaggaga ctgtcaccat ctcaccaaga accctgtttg
     1981 tgtcctctga aggccacacc ttcctggcag cagacttttc acagattgaa ttgcgcattc
     2041 ttgcacattt atctggggat ccagagcttt tgaagttatt ccaggaatct gaaagagatg
     2101 atgtattttc taccctgact tcacagtgga aggacattcc catagagcgt gtgacacaca
     2161 tggacagaga acaaaccaag aaagtggtgt actccgtggt ctatggagct gggaaagagc
     2221 ggcttgctgc ttgccttgga gtcactgttt tggaagccac ccactttttg gagagatttt
     2281 tgcagaagta caagaaaatc aaggactttg cccagacagt tattggccag tgtcacagtg
     2341 caggctatgt gacatccatc ttgggtagaa ggaggcctct cccgaggatc tgtgcacagg
     2401 accagcagct tcgggcacaa gcagagcgac aggcagtaaa ctttgtggta caaggctcag
     2461 ccgcagacct ctgtaagcta gccatgattc gcatctctac tgcagtagcc acatccccga
     2521 ccttgacagc caggttagtt gcccaaattc atgatgagct gctgtttgaa gtagaagaca
     2581 ctcaggtccc tgagtttgca gctcttgtca gaaggatcat ggagtccttg caacaggtac
     2641 agaccctgga actgcagctg caggtgcccc taaaagtgaa cttgagtgtt ggccgctcat
     2701 ggggacacct gaccccactg caggagatcc tgggctcagc ctgacctcag ccacaatcca
     2761 gctccagtga tgaacctgtt ccctactcac atgaaggaca ttttttttca tcctatgttt
     2821 gtctgtatcc ctgggcaaca gtggaatgag agagtttgtt ttcaccaatc catcctacag
     2881 cctttccccg tattacagac ttgtactctg tgagggctaa actgatacag accacacaaa
     2941 acctttgtgg gacaactggg acacctgaag taaaagccta attagaagct gtct
//
