LOCUS       NM_017401               2943 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus polymerase (DNA directed), mu (Polm), mRNA.
ACCESSION   NM_017401
VERSION     NM_017401.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Selva-Clemente,J., Marcos,P., Gonzalez-Fuentes,J.,
            Villaseca-Gonzalez,N., Lagartos-Donate,M.J., Insausti,R. and
            Arroyo-Jimenez,M.M.
  TITLE     Interneurons in the CA1 stratum oriens expressing alphaTTP may play
            a role in the delayed-ageing Pol mu mouse model
  JOURNAL   Mol Cell Neurosci 130, 103960 (2024)
   PUBMED   39179163
  REMARK    GeneRIF: Interneurons in the CA1 stratum oriens expressing alphaTTP
            may play a role in the delayed-ageing Pol mu mouse model.
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2943)
  AUTHORS   Alvarez-Lindo,N., Baleriola,J., de Los Rios,V., Suarez,T. and de la
            Rosa,E.J.
  TITLE     RAG-2 deficiency results in fewer phosphorylated histone H2AX foci,
            but increased retinal ganglion cell death and altered axonal growth
  JOURNAL   Sci Rep 9 (1), 18486 (2019)
   PUBMED   31811168
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2943)
  AUTHORS   Loc'h,J., Gerodimos,C.A., Rosario,S., Tekpinar,M., Lieber,M.R. and
            Delarue,M.
  TITLE     Structural evidence for an in trans base selection mechanism
            involving Loop1 in polymerase mu at an NHEJ double-strand break
            junction
  JOURNAL   J Biol Chem 294 (27), 10579-10595 (2019)
   PUBMED   31138645
  REMARK    GeneRIF: The atomic structure of an NHEJ junction with a Pol x
            construct that mimics Pol mu in a reconstituted system explained
            the distinctive properties of Pol mu compared with TdT. The
            structure suggested a mechanism of base selection relying on Loop1
            and taking instructions via the in trans templating base
            independently of the primer strand.
REFERENCE   5  (bases 1 to 2943)
  AUTHORS   Escudero,B., Herrero,D., Torres,Y., Canon,S., Molina,A.,
            Carmona,R.M., Suela,J., Blanco,L., Samper,E. and Bernad,A.
  TITLE     Polmu deficiency induces moderate shortening of P53-/- mouse
            lifespan and modifies tumor spectrum
  JOURNAL   DNA Repair (Amst) 54, 40-45 (2017)
   PUBMED   28460268
  REMARK    GeneRIF: A role for PolMU in the prevention of sarcomagenesis on a
            murine P53-deficient background was identified.
REFERENCE   6  (bases 1 to 2943)
  AUTHORS   Lucas,D., Lain de Lera,T., Gonzalez,M.A., Ruiz,J.F., Dominguez,O.,
            Casanova,J.C., Martinez-A,C., Blanco,L. and Bernad,A.
  TITLE     Polymerase mu is up-regulated during the T cell-dependent immune
            response and its deficiency alters developmental dynamics of spleen
            centroblasts
  JOURNAL   Eur J Immunol 35 (5), 1601-1611 (2005)
   PUBMED   15789338
REFERENCE   7  (bases 1 to 2943)
  AUTHORS   Bertocci,B., De Smet,A., Berek,C., Weill,J.C. and Reynaud,C.A.
  TITLE     Immunoglobulin kappa light chain gene rearrangement is impaired in
            mice deficient for DNA polymerase mu
  JOURNAL   Immunity 19 (2), 203-211 (2003)
   PUBMED   12932354
REFERENCE   8  (bases 1 to 2943)
  AUTHORS   Bertocci,B., De Smet,A., Flatter,E., Dahan,A., Bories,J.C.,
            Landreau,C., Weill,J.C. and Reynaud,C.A.
  TITLE     Cutting edge: DNA polymerases mu and lambda are dispensable for Ig
            gene hypermutation
  JOURNAL   J Immunol 168 (8), 3702-3706 (2002)
   PUBMED   11937519
  REMARK    GeneRIF: Pol mu is not required for normal Ig gene hypermutation.
REFERENCE   9  (bases 1 to 2943)
  AUTHORS   Aoufouchi,S., Flatter,E., Dahan,A., Faili,A., Bertocci,B.,
            Storck,S., Delbos,F., Cocea,L., Gupta,N., Weill,J.C. and
            Reynaud,C.A.
  TITLE     Two novel human and mouse DNA polymerases of the polX family
  JOURNAL   Nucleic Acids Res 28 (18), 3684-3693 (2000)
   PUBMED   10982892
REFERENCE   10 (bases 1 to 2943)
  AUTHORS   Dominguez,O., Ruiz,J.F., Lain de Lera,T., Garcia-Diaz,M.,
            Gonzalez,M.A., Kirchhoff,T., Martinez-A,C., Bernad,A. and Blanco,L.
  TITLE     DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA
            mutator in eukaryotic cells
  JOURNAL   EMBO J 19 (7), 1731-1742 (2000)
   PUBMED   10747040
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ130894.1, AF176098.1 and BC056996.1.
            
            On Apr 7, 2009 this sequence version replaced NM_017401.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC056213.1, AF176098.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-456               CJ130894.1         2-457
            457-1083            AF176098.1         230-856
            1084-2943           BC056996.1         2731-4590
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.87 cM"
     gene            1..2943
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /note="polymerase (DNA directed), mu"
                     /db_xref="GeneID:54125"
                     /db_xref="MGI:MGI:1860191"
     exon            1..456
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..1756
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /EC_number="2.7.7.7"
                     /note="DNA polymerase mu; DNA-directed DNA polymerase mu;
                     pol Mu; terminal transferase"
                     /codon_start=1
                     /product="DNA-directed DNA/RNA polymerase mu"
                     /protein_id="NP_059097.2"
                     /db_xref="CCDS:CCDS24405.1"
                     /db_xref="GeneID:54125"
                     /db_xref="MGI:MGI:1860191"
                     /translation="MLPKRRRVRAGSPHSAVASSTPPSVVRFPDVAIYLAEPRMGRSR
                     RAFLTRLARSKGFRVLDAYSSKVTHVVMEGTSAKEAICWQKNMDALPTGCPQPALLDI
                     SWFTESMAAGQPVPEEGRHHLEVAEPRKEPPVSASMPAYACQRPSPLTHHNTLLSEAL
                     ETLAEAAGFEANEGRLLSFSRAASVLKSLPCPVASLSQLHGLPYFGEHSTRVIQELLE
                     HGTCEEVKQVRCSERYQTMKLFTQVFGVGVKTANRWYQEGLRTLDELREQPQRLTQQQ
                     KAGLQYYQDLSTPVRRADAEALQQLIEAAVRQTLPGATVTLTGGFRRGKLQGHDVDFL
                     ITHPEEGQEVGLLPKVMSCLQSQGLVLYHQYHRSHLADSAHNLRQRSSTMDAFERSFC
                     ILGLPQPQQAALAGALPPCPTWKAVRVDLVVTPSSQFPFALLGWTGSQFFERELRRFS
                     RQEKGLWLNSHGLFDPEQKRVFHATSEEDVFRLLGLKYLPPEQRNA"
     exon            457..637
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            638..736
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            737..907
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            908..979
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1100
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1233
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1336
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1585
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1669
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..2925
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2902..2907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /note="hexamer: AATAAA"
     polyA_site      2925
                     /gene="Polm"
                     /gene_synonym="B230309I03Rik; Tdt-N"
                     /note="major polyA site"
ORIGIN      
        1 agtacgaggc gaagccccgc ctacgggccc aggctactct aaggtctggg aaaccactgg
       61 ggctttctat gagagtagtt caaataagaa tcctcgggcg ttatttgcat agaactgggg
      121 cttccgatgg gaagccaaaa gcatcctaga gggcgtctcg tttgcataga gcagacacca
      181 cttcctctct cctccttccg ccttctttca tgaccctgcc ttccttcgtt ctgcggcttc
      241 ctgtgcttcc agagttggtg cccagatgct tccgaagcga cggcgagtgc gagctgggtc
      301 cccacacagc gcggtagcct cttccacgcc gccctcggtg gtgcgcttcc cggatgtggc
      361 catctacctt gcggagccgc gaatgggccg cagccgccgg gccttcctca cccgcctggc
      421 gcggtccaaa ggcttccgcg tcctagatgc ttacagctca aaggtgacac atgtggtgat
      481 ggaagggacc tcagccaagg aggccatctg ctggcagaag aacatggatg ctctccccac
      541 aggctgccca cagccagctc tgctagatat tagctggttt acagaaagca tggcagctgg
      601 gcagcctgtc cctgaggagg gccggcacca cctggaggta gctgagccca ggaaggagcc
      661 cccagtctca gcgtcgatgc cagcttatgc ctgtcagcgc ccctcacctc tcacacacca
      721 taacactctc ctctcagagg ctctggagac gctagcggag gcagcgggct tcgaagccaa
      781 cgagggccgt cttctctcct tctccagagc ggcctcggtg ctcaagtccc tgccctgccc
      841 tgttgcatct ttgagccagc tgcatgggct gccctacttt ggagaacatt ccactagagt
      901 aatccaggag ctgctggagc atggaacatg tgaagaagtg aaacaagtcc gttgctcaga
      961 aaggtaccag accatgaagc tcttcaccca ggtcttcggg gttggggtga agactgccaa
     1021 ccggtggtac caggaaggac tacgaaccct ggatgagctc agagagcagc cccagagact
     1081 gacacagcag cagaaagcag ggctccaata ttaccaggac ctgagcactc cagttaggcg
     1141 agctgatgcc gaggctctgc agcagttgat agaggcagct gtgagacaga ccctgcccgg
     1201 agccactgtc acactgactg gcggtttccg aagggggaag ttacaaggcc atgatgtaga
     1261 cttccttatc acccacccag aggaggggca agaagtaggg ctgctgccca aagtgatgag
     1321 ctgcctacag agccagggac tcgtcctgta tcaccagtac caccgcagcc atttggcaga
     1381 ctcagcccac aacctgcggc agcggagctc caccatggat gcttttgaga ggagtttctg
     1441 catcttgggt ttgccacaac cccaacaggc agctttagcg ggggccctgc ctccctgccc
     1501 aacttggaaa gctgtgaggg tagatcttgt ggtcacgccc agcagccagt tcccctttgc
     1561 ccttctgggc tggactggct cccagttctt tgagcgggag ctacggcggt tcagccgtca
     1621 agagaagggg ctgtggctta acagccatgg gctgtttgat cctgagcaga agagagtttt
     1681 ccatgcaact tctgaggaag atgttttcag actcctgggc ctcaagtacc ttcctccaga
     1741 gcagagaaat gcctgaactg agaaatgcct gtgggctgcc acacccatcc atacaaaaat
     1801 gaggggctgc ctcttgcttg accaggaatg tttctagaca ggaacactgc caccccatgg
     1861 cctcacctgt gcaaatggag ttttcacctc ctgggacatt agtagagtgt gaccccgagt
     1921 caactcagct ttcaacctcc acacacctcg cagtcagggg cctttagcag ttcatacatc
     1981 acggacctat gtcagagcct gaagtcttgg ttctgggcca gctgtggttg ctgtgtctgc
     2041 tgcagagtac tccgttctgc gtgccagaaa agagagacct atgttcccgc cctgggcttg
     2101 atatgggaag tgtcattcca gcagtgaggt gttgtgcaga agtaagacgc ctgagtgcct
     2161 gctgcagtag gaggtggccc aggacagcaa cctccagtgc tggtgctgtt ggcaccacca
     2221 cccgaagaca tgggtacctg taccagactt cataagcagc tcatctccag aatcggcccc
     2281 acatgtgtcc tctctctgta gctaccaatc ccccgcattc cagcctaggc taagccgggc
     2341 ctgcctaccc acaatctcct gtgcagcctc agcccctgca gtaccttctg ggaaggctgg
     2401 tttcctagca cagagactgt agctcagcag catcagtggg cagcagcaga gcgctcttcc
     2461 ttcttatcct gagattgtga gctgcgtggt ccctggtcct gctcctctgc tctgccacgt
     2521 ctaactgccc tgcagcagtt tcgaacctgc aatccttatg caggcctgtg accctgcagt
     2581 cctcaggcag gccctgtgac tctggtgttt ctgttatctc ttccagtctc agctgtaccc
     2641 ctccagataa gccttccccc acttacccag ttcactcctg gtccctgttt cccagctctt
     2701 tgtgaccaac ttcttcacct tctctgggct ggaagcacta aagtgtcaca gtgtctttgt
     2761 atctccaagt gtcgagacct ataaagtccc tttccttcct gtacacttag tgcccactgt
     2821 ccctgcctcc ttcagggcct gacagatgcg ggcctcctag atcagtcagc agattaacaa
     2881 gtatttttaa aattaacatt caataaagtg gattattttt ttggaaaaaa aaaaaaaaaa
     2941 aaa
//
