LOCUS       NM_020032               2371 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus polymerase (DNA directed), lambda (Poll), transcript
            variant 1, mRNA.
ACCESSION   NM_020032
VERSION     NM_020032.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   McCallum-Loudeac,J., Moody,E., Williams,J., Johnstone,G.,
            Sircombe,K.J., Clarkson,A.N. and Wilson,M.J.
  TITLE     Deletion of a conserved genomic region associated with adolescent
            idiopathic scoliosis leads to vertebral rotation in mice
  JOURNAL   Hum Mol Genet 33 (9), 787-801 (2024)
   PUBMED   38280229
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Cova,G., Glaser,J., Schopflin,R., Prada-Medina,C.A., Ali,S.,
            Franke,M., Falcone,R., Federer,M., Ponzi,E., Ficarella,R.,
            Novara,F., Wittler,L., Timmermann,B., Gentile,M., Zuffardi,O.,
            Spielmann,M. and Mundlos,S.
  TITLE     Combinatorial effects on gene expression at the Lbx1/Fgf8 locus
            resolve split-hand/foot malformation type 3
  JOURNAL   Nat Commun 14 (1), 1475 (2023)
   PUBMED   36928426
  REMARK    Erratum:[Nat Commun. 2023 May 18;14(1):2839. doi:
            10.1038/s41467-023-38736-7.. PMID: 37202388]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2371)
  AUTHORS   Thapar,U. and Demple,B.
  TITLE     Deployment of DNA polymerases beta and lambda in single-nucleotide
            and multinucleotide pathways of mammalian base excision DNA repair
  JOURNAL   DNA Repair (Amst) 76, 11-19 (2019)
   PUBMED   30763888
  REMARK    GeneRIF: DNA polymerases beta and lambda in single-nucleotide and
            multinucleotide pathways of mammalian base excision DNA repair
REFERENCE   4  (bases 1 to 2371)
  AUTHORS   Belousova,E.A. and Lavrik,O.I.
  TITLE     DNA polymerases beta and lambda and their roles in cell
  JOURNAL   DNA Repair (Amst) 29, 112-126 (2015)
   PUBMED   25715666
  REMARK    Review article
REFERENCE   5  (bases 1 to 2371)
  AUTHORS   Bebenek,K., Pedersen,L.C. and Kunkel,T.A.
  TITLE     Structure-function studies of DNA polymerase lambda
  JOURNAL   Biochemistry 53 (17), 2781-2792 (2014)
   PUBMED   24716527
  REMARK    Review article
REFERENCE   6  (bases 1 to 2371)
  AUTHORS   Velasco-Miguel,S., Richardson,J.A., Gerlach,V.L., Lai,W.C., Gao,T.,
            Russell,L.D., Hladik,C.L., White,C.L. and Friedberg,E.C.
  TITLE     Constitutive and regulated expression of the mouse Dinb (Polkappa)
            gene encoding DNA polymerase kappa
  JOURNAL   DNA Repair (Amst) 2 (1), 91-106 (2003)
   PUBMED   12509270
REFERENCE   7  (bases 1 to 2371)
  AUTHORS   Bertocci,B., De Smet,A., Flatter,E., Dahan,A., Bories,J.C.,
            Landreau,C., Weill,J.C. and Reynaud,C.A.
  TITLE     Cutting edge: DNA polymerases mu and lambda are dispensable for Ig
            gene hypermutation
  JOURNAL   J Immunol 168 (8), 3702-3706 (2002)
   PUBMED   11937519
  REMARK    GeneRIF: Pol lambda is not required for normal Ig gene
            hypermutation.
REFERENCE   8  (bases 1 to 2371)
  AUTHORS   Kobayashi,Y., Watanabe,M., Okada,Y., Sawa,H., Takai,H.,
            Nakanishi,M., Kawase,Y., Suzuki,H., Nagashima,K., Ikeda,K. and
            Motoyama,N.
  TITLE     Hydrocephalus, situs inversus, chronic sinusitis, and male
            infertility in DNA polymerase lambda-deficient mice: possible
            implication for the pathogenesis of immotile cilia syndrome
  JOURNAL   Mol Cell Biol 22 (8), 2769-2776 (2002)
   PUBMED   11909969
  REMARK    GeneRIF: Hydrocephalus, situs inversus, chronic sinusitis, and male
            infertility in DNA polymerase lambda-deficient mice: possible
            implication for the pathogenesis of immotile cilia syndrome.
REFERENCE   9  (bases 1 to 2371)
  AUTHORS   Aoufouchi,S., Flatter,E., Dahan,A., Faili,A., Bertocci,B.,
            Storck,S., Delbos,F., Cocea,L., Gupta,N., Weill,J.C. and
            Reynaud,C.A.
  TITLE     Two novel human and mouse DNA polymerases of the polX family
  JOURNAL   Nucleic Acids Res 28 (18), 3684-3693 (2000)
   PUBMED   10982892
REFERENCE   10 (bases 1 to 2371)
  AUTHORS   Garcia-Diaz,M., Dominguez,O., Lopez-Fernandez,L.A., de Lera,L.T.,
            Saniger,M.L., Ruiz,J.F., Parraga,M., Garcia-Ortiz,M.J.,
            Kirchhoff,T., del Mazo,J., Bernad,A. and Blanco,L.
  TITLE     DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA
            polymerase with a potential role in meiosis
  JOURNAL   J Mol Biol 301 (4), 851-867 (2000)
   PUBMED   10966791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY315476.1, BY270661.1, BG080424.2, AK003392.1 and BE949923.1.
            
            On Aug 26, 2016 this sequence version replaced NM_020032.2.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the longer isoform (1). Both variants 1 and
            2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ131889.1, BC004767.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                BY315476.1         2-28
            28-364              BY270661.1         1-337
            365-885             BG080424.2         1-521
            886-2354            AK003392.1         1-1469
            2355-2371           BE949923.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 38.75 cM"
     gene            1..2371
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /note="polymerase (DNA directed), lambda"
                     /db_xref="GeneID:56626"
                     /db_xref="MGI:MGI:1889000"
     exon            1..79
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            80..216
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1826
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase lambda; pol Lambda"
                     /codon_start=1
                     /product="DNA polymerase lambda isoform 1"
                     /protein_id="NP_064416.1"
                     /db_xref="CCDS:CCDS29861.1"
                     /db_xref="GeneID:56626"
                     /db_xref="MGI:MGI:1889000"
                     /translation="MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLR
                     AHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQL
                     PPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLP
                     RTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYP
                     TPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALG
                     YAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLE
                     LFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAE
                     IEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLR
                     QQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSA
                     HFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYR
                     EPAERDW"
     exon            217..511
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            512..671
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            672..989
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1163
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1292
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1461
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..2357
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2334..2339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2357
                     /gene="Poll"
                     /gene_synonym="1110003P06Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtattctggg aactgatttt tttttttttt tttcccctct accccttcct cccccaatcc
       61 gttttcattg gctcccatga aaggccagga tccagcgccc ttcaatggac cctcagggca
      121 tcgtgaaggc ctttcccaag cggaagaaaa gtcatgcaga tctatcttca aaagcacttg
      181 caaagattcc caaaagggag gtgggagaag ctagaggatg gctgagctcc ctgagagccc
      241 acattatgcc cgctggcatt ggacgtgccc gggctgaact ctttgagaag cagattatcc
      301 accatggcgg ccaggtgtgc tctgcccagg ccccaggagt cactcacatt gtggtggatg
      361 aagacatgga ctatgagcgg gctctccggc tcctcaggtt gccccagcta ccccctggtg
      421 cccagctggt gaagtccacc tggctgagct tgtgcctgca ggagggaagg ctgacagaca
      481 cggaaggatt cagccttccc atgcccaaga ggtccttgga tgaaccacag cccagcaagt
      541 caggccaaga tgcttctgct cctggcaccc agcgggattt acccaggacc accctttctc
      601 tttcccctcc tcacaccaga gctgtatctc ctcccccaac ggcagaaaag ccatcaagaa
      661 cccaagccca gctcagctca gaggatgaaa ccagcgatgg agaagggccc caggttagct
      721 cagcagatct gcaagccttg atcactgggc actaccccac tccccctgag gaagatggtg
      781 ggcctgaccc agcaccagaa gctctggata aatgggtctg tgcacagccc tcgagccaga
      841 aggcaactaa ctacaatctg cacatcacag agaagctgga agtgctggct aaagcctaca
      901 gtgtccaggg agacaagtgg agggccctgg gctatgcaaa ggccatcaat gctctcaaga
      961 gcttccacaa gcctgtcagc tcctaccagg aggcctgtag catcccggga attggcaagc
     1021 ggatggcgga gaaggtcatg gagatcctgg agagtgggca tctgcggaag ctagaccaca
     1081 tcagtgacag tgtgcctgtc ttggagctct tctccaacat ctggggagct gggacgaaga
     1141 ctgcccagat gtggtaccat cagggcttcc gaaacctaga agatctccaa agcctgggct
     1201 ccctgaccgc tcagcaggcc attggcttga agcactacga tgacttcctg gaccgcatgc
     1261 ccagggagga ggctgcggaa attgagcaga cggtccggat atcagcccag gccttcaacc
     1321 ctgggctgct gtgtgtggct tgtggctctt accgacgggg gaagatgacg tgcggggatg
     1381 tggatgtact cattactcac cccgatggcc gatcccaccg gggcatcttc agctgcctcc
     1441 ttgatagcct tcggcagcaa gggttcctca cagatgactt ggtgagccag gaggagaacg
     1501 gccaacagca gaaatacctg ggtgtgtgcc ggctcccagg gcccgggaag cgccaccggc
     1561 gactggacat catcgtggta ccctactgtg agtttgcctg tgccctgctc tacttcaccg
     1621 gctctgccca cttcaaccgg tccatgagag ctctggccaa gaccaagggc atgagcctgt
     1681 cggagcatgc gctcagtgca gctgtagtcc ggaacagcca aggtgtcaag gtggggcctg
     1741 gacaagtgct gcccaccccc acggagaagg acgtcttcaa gcacttaggc ctgccctacc
     1801 gggagccagc tgaacgggac tggtgaccta ggacttggag caaggggaac catgttgaat
     1861 gaactgactc atgtagccat ctcccaccct cttagctgtg cctcgctttg taggttacgc
     1921 ttgccctgag tgaccaagct aggagctttg ggcctgcggg gaagagccag tctgattctg
     1981 agagcccgca agctctctct cctggacagg atttatgctg ctgtcccctc tcctcccagt
     2041 gacaggtttt cacacggccc cttccctgtt caagcagaac aggtggctgg tttcaaggcg
     2101 gtttcctgca ctgaaggccc gagcttccct ttccacccca agactgatgt agctgtgtgg
     2161 ggtcagcagg ggctgcagga gagggaagca gctgtgggct cagcgtcttc cgtgagcctt
     2221 cacagtagag aaagccatgt ggattctctc acccgctcac ttcctgtgca gctggagctg
     2281 ctgggggtgg cctgtccaga ccatgagggt ggcttctaag ggccctgggg cctaataaag
     2341 tgcttctgac attcaaaaaa aaaaaaaaaa a
//
