LOCUS       NM_011972               2479 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus polymerase (DNA directed), iota (Poli), transcript
            variant 2, mRNA.
ACCESSION   NM_011972
VERSION     NM_011972.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Cao,R., Liu,Y., Wei,K., Jin,N., Liang,Y., Ao,R., Pan,W., Wang,X.,
            Wang,X., Zhang,L. and Xie,J.
  TITLE     Genes related to neural tube defects and glioblastoma
  JOURNAL   Sci Rep 15 (1), 3777 (2025)
   PUBMED   39885289
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2479)
  AUTHORS   Gao,A., Zhang,M., Zhu,S.Q., Zou,S., Chen,H., Li,X., He,C., Zhou,L.,
            Mei,Y., Ding,W., Zhou,J., Zhou,Y. and Cao,Y.
  TITLE     DNA polymerase iota promotes EMT and metastasis of esophageal
            squamous cell carcinoma by interacting with USP7 to stabilize
            HIF-1alpha
  JOURNAL   Cell Death Dis 15 (2), 171 (2024)
   PUBMED   38402183
  REMARK    GeneRIF: DNA polymerase iota promotes EMT and metastasis of
            esophageal squamous cell carcinoma by interacting with USP7 to
            stabilize HIF-1alpha.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2479)
  AUTHORS   Kazachenko,K.Y., Miropolskaya,N.A., Gening,L.V., Tarantul,V.Z. and
            Makarova,A.V.
  TITLE     Alternative splicing at exon 2 results in the loss of the catalytic
            activity of mouse DNA polymerase iota in vitro
  JOURNAL   DNA Repair (Amst) 50, 77-82 (2017)
   PUBMED   28077248
  REMARK    GeneRIF: Exon 2 deletion abrogates both the DNA polymerase and dRP
            lyase activities of Pol iota in the presence of either Mg(2+) or
            Mn(2+) ions. Thus, 129-derived strains of mice express
            catalytically inactive alternatively spliced Pol iota variant.
REFERENCE   5  (bases 1 to 2479)
  AUTHORS   Frank,E.G., McDonald,J.P., Yang,W. and Woodgate,R.
  TITLE     Mouse DNA polymerase iota lacking the forty-two amino acids encoded
            by exon-2 is catalytically inactive in vitro
  JOURNAL   DNA Repair (Amst) 50, 71-76 (2017)
   PUBMED   28077247
  REMARK    GeneRIF: Mouse DNA polymerase iota lacking exon 2 (42 amino acids)
            is catalytically inactive in vitro.
REFERENCE   6  (bases 1 to 2479)
  AUTHORS   McIntyre,J., McLenigan,M.P., Frank,E.G., Dai,X., Yang,W., Wang,Y.
            and Woodgate,R.
  TITLE     Posttranslational Regulation of Human DNA Polymerase iota
  JOURNAL   J Biol Chem 290 (45), 27332-27344 (2015)
   PUBMED   26370087
REFERENCE   7  (bases 1 to 2479)
  AUTHORS   McDonald,J.P., Frank,E.G., Plosky,B.S., Rogozin,I.B., Masutani,C.,
            Hanaoka,F., Woodgate,R. and Gearhart,P.J.
  TITLE     129-derived strains of mice are deficient in DNA polymerase iota
            and have normal immunoglobulin hypermutation
  JOURNAL   J Exp Med 198 (4), 635-643 (2003)
   PUBMED   12925679
  REMARK    GeneRIF: Data suggest that either DNA polymerase iota does not
            participate in hypermutation, or its role is nonessential and can
            be readily assumed by another low-fidelity polymerase.
REFERENCE   8  (bases 1 to 2479)
  AUTHORS   Lee,G.H., Nishimori,H., Sasaki,Y., Matsushita,H., Kitagawa,T. and
            Tokino,T.
  TITLE     Analysis of lung tumorigenesis in chimeric mice indicates the
            Pulmonary adenoma resistance 2 (Par2) locus to operate in the
            tumor-initiation stage in a cell-autonomous manner: detection of
            polymorphisms in the Poli gene as a candidate for Par2
  JOURNAL   Oncogene 22 (15), 2374-2382 (2003)
   PUBMED   12700672
REFERENCE   9  (bases 1 to 2479)
  AUTHORS   Velasco-Miguel,S., Richardson,J.A., Gerlach,V.L., Lai,W.C., Gao,T.,
            Russell,L.D., Hladik,C.L., White,C.L. and Friedberg,E.C.
  TITLE     Constitutive and regulated expression of the mouse Dinb (Polkappa)
            gene encoding DNA polymerase kappa
  JOURNAL   DNA Repair (Amst) 2 (1), 91-106 (2003)
   PUBMED   12509270
REFERENCE   10 (bases 1 to 2479)
  AUTHORS   McDonald,J.P., Rapic-Otrin,V., Epstein,J.A., Broughton,B.C.,
            Wang,X., Lehmann,A.R., Wolgemuth,D.J. and Woodgate,R.
  TITLE     Novel human and mouse homologs of Saccharomyces cerevisiae DNA
            polymerase eta
  JOURNAL   Genomics 60 (1), 20-30 (1999)
   PUBMED   10458907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134831.3.
            
            On Mar 28, 2022 this sequence version replaced NM_011972.2.
            
            Transcript Variant: This variant (2) encodes two different
            proteins, isoform 2 (long) and isoform 2 (short). Variant 2 encodes
            the longest protein [isoform 2 (long)]. However, this full length
            (737 aa) form is a minor form. A downstream translation start site
            is more commonly used, resulting in a 717 aa protein [isoform 2
            (short)].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF151691.1, AK145244.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC134831.3         130779-130912       c
            135-260             AC134831.3         129293-129418       c
            261-425             AC134831.3         127148-127312       c
            426-578             AC134831.3         125967-126119       c
            579-815             AC134831.3         123754-123990       c
            816-994             AC134831.3         123293-123471       c
            995-1086            AC134831.3         122441-122532       c
            1087-1214           AC134831.3         117968-118095       c
            1215-1417           AC134831.3         117385-117587       c
            1418-2479           AC134831.3         109274-110335       c
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 44.48 cM"
     gene            1..2479
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="polymerase (DNA directed), iota"
                     /db_xref="GeneID:26447"
                     /db_xref="MGI:MGI:1347081"
     exon            1..134
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..2248
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /EC_number="2.7.7.7"
                     /note="isoform 2 (long) is encoded by transcript variant
                     2; RAD30 homolog B; DNA polymerase iota"
                     /codon_start=1
                     /product="DNA polymerase iota isoform 2 (long)"
                     /protein_id="NP_036102.2"
                     /db_xref="CCDS:CCDS29334.2"
                     /db_xref="GeneID:26447"
                     /db_xref="MGI:MGI:1347081"
                     /translation="MGVESEEEGGPAEEEDAPRAMEPLHAGAAGSSRAVCSQGPPTQI
                     SSSRVIVHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVR
                     DAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRL
                     QQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQLGLTGCAG
                     VAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGIN
                     SVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSE
                     VEAKAKIEELLSSLLTRVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLG
                     TGNHDSMPPLIDILMKLFRNMVNVKMPFHLTLMSVCFCNLKALSSAKKGPMDCYLTSL
                     STPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIESTGTGESPLDATCFPKEKDT
                     SDLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSCPLHASRGVLSFFS
                     TKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKD
                     EQTSQGPTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLE
                     SHQGLESRELDSAEEKLPFPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR"
     exon            135..260
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            261..425
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            426..578
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            579..815
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            816..994
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1086
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1214
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1417
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..2479
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2455..2460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="hexamer: AATAAA"
     polyA_site      2477
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="major polyA site"
ORIGIN      
        1 gcagcagctg cggtgcggtt ggcccgaagg gaagatgggg gtggagtcgg aggaggaagg
       61 cggccccgcg gaggaagaag acgctcctcg tgccatggag cccttgcacg cgggggcggc
      121 gggcagctcg cgggcagttt gcagtcaagg gccacctaca caaatctctt cgtcgagagt
      181 catagtccac gtagatctgg attgctttta tgcccaggtc gaaatgatct ccaacccaga
      241 attaaaggac agacctttag gtgtgcaaca gaaatacttg gtggttacct gcaactatga
      301 agccaggaaa ctgggtgtga ggaagcttat gaatgtgaga gatgcgaagg aaaagtgtcc
      361 tcagctggtc ctcgtgaacg gagaagacct gagccgctac agagagatgt cctataaggt
      421 cacagaattg ttggaggaat ttagtccagc tgttgagaga cttggatttg atgaaaattt
      481 tgtggatcta acagaaatgg tggaaaaaag actccagcag cttccaagtg aggaggttcc
      541 gtcagtgact gtgttcggcc atgtttacaa taaccagtct gtgaacctac acaacatcat
      601 gcatcggaga cttgtggttg gatcgcagat tgcagcagag atgcgggaag ccatgtataa
      661 tcagctcggg ctcacaggct gcgccggagt ggctcctaat aaactcttgg caaagttagt
      721 gtctggggtt tttaaaccaa atcagcagac ggtcttacta cctgaaagtt gtcaacatct
      781 cattcacagt ttgaaccaca taaaggaaat tcctggtatc ggctataaaa ctgccaagcg
      841 tctcgaagtt ctgggaatca atagtgttca tgatctccaa acctttccaa tcaaaacatt
      901 agaaaaagaa ttaggaattg caattgctca gcgtatccag cagctcagtt ttggagagga
      961 caagtctccc gtcacaccgt cagggccacc acagtccttt agtgaagaag atacgtttaa
     1021 aaagtgttcc tcagaagtgg aagctaaagc taagattgaa gaattacttt ccagcctttt
     1081 gaccagagta tgccaggatg gaaggaagcc ccatacagta agattagtca tccgtcggta
     1141 ctctgacaaa cactgtaatc gagagagtcg tcagtgccct atcccatccc acgtcattca
     1201 gaagttaggg acaggaaatc atgactccat gcctcccctg attgatatcc ttatgaaact
     1261 tttccgaaat atggtgaacg tgaagatgcc ctttcacctg actcttatga gcgtgtgctt
     1321 ctgcaacctg aaagccctga gcagtgctaa gaaagggcct atggactgct acctaacgtc
     1381 cctctcaacc cctgcctaca ccgacaagcg cgcctttaaa gtgaaggaca cccacacgga
     1441 agactctcac aaggagaaag aagcaaactg ggattgtcta ccaagtagaa gaatcgaaag
     1501 cacaggaacg ggggagtctc cgttggatgc cacctgtttt cctaaagaaa aagacacaag
     1561 tgacttgccc ctccaggcac tgccagaggg tgttgatcaa gaagtcttta agcaacttcc
     1621 agcagatatt caagaagaaa tcctttctgg aaaatctaga gaaaatctaa aagggaaagg
     1681 aagtttaagt tgtccgctgc atgcctctag aggagtattg tctttctttt ctacaaagca
     1741 aatgcaagcc agtcgcttaa gccccagaga tactgcgctc cctagcaagc gggtatcagc
     1801 tgcatctccc tgtgagccgg gaacgtcagg actgagcccc gggagcacct cccatccatc
     1861 gtgcggaaag gactgttcct attacataga cagccagtta aaggatgagc aaacgagtca
     1921 aggccctact gagtctcaag gatgccagtt ttccagcacg aaccctgctg tttctggttt
     1981 ccattccttt cctaatctgc agactgaaca gctcttctcc acacatcgca ctgtagacag
     2041 ccacaagcag acagccaccg cctctcacca gggactagaa agtcaccagg gactagaaag
     2101 cagagagctg gattctgcgg aagaaaagct tcctttccca ccagacattg atccgcaagt
     2161 tttctatgag cttccagaag aggtccaaaa ggaactgatg gcggaatggg agagagctgg
     2221 agctgcgcgc ccctcggcgc acagataagt ccagaaagcc agggagtatg agtccccagg
     2281 tcgccagggc ataaccaaat agaaatagtc agtggtgtgg aaatccgatg taagatgtaa
     2341 tagataaggc aaaagtgggc acaggaagga aattctagta caaagggaaa atagccataa
     2401 tgtggtaatg taaaaaatgc tatctcccta ttatttctgt tttatatcat tgtcaataaa
     2461 aggaatatca tggtcaaca
//
